Restriction map for
pF1KB0971
(Linear) (Six-base) MAPSORT of: KIBB0971.seq Check: 4510 from: 1 to: 627
KIBB0971.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 19 627
Size: 19 608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 627
Size: 547 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 627
Size: 51 576
AlwNI CAG_nnn'CTG
Cuts at: 0 111 627
Size: 111 516
ApaI G_GGCC'C
Cuts at: 0 544 627
Size: 544 83
ApaLI G'TGCA_C
Cuts at: 0 220 548 627
Size: 220 328 79
Fragments arranged by size:
328 220 79
AvaI C'yCGr_G
Cuts at: 0 61 86 627
Size: 61 25 541
Fragments arranged by size:
541 61 25
BanI G'GyrC_C
Cuts at: 0 57 69 627
Size: 57 12 558
Fragments arranged by size:
558 57 12
BanII G_rGCy'C
Cuts at: 0 7 544 627
Size: 7 537 83
Fragments arranged by size:
537 83 7
BbeI G_GCGC'C
Cuts at: 0 61 73 627
Size: 61 12 554
Fragments arranged by size:
554 61 12
BbvCI CC'TCA_GC
Cuts at: 0 104 627
Size: 104 523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 138 172 627
Size: 138 34 455
Fragments arranged by size:
455 138 34
BglI GCCn_nnn'nGGC
Cuts at: 0 46 91 627
Size: 46 45 536
Fragments arranged by size:
536 46 45
Bme1580I G_kGCm'C
Cuts at: 0 224 544 552 627
Size: 224 320 8 75
Fragments arranged by size:
320 224 75 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 199 627
Size: 199 428
Bpu10I CC'TnA_GC
Cuts at: 0 104 627
Size: 104 523
BsaHI Gr'CG_yC
Cuts at: 0 58 70 627
Size: 58 12 557
Fragments arranged by size:
557 58 12
BsaWI w'CCGG_w
Cuts at: 0 251 328 627
Size: 251 77 299
Fragments arranged by size:
299 251 77
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 627
Size: 79 548
BseYI C'CCAG_C
Cuts at: 0 467 533 627
Size: 467 66 94
Fragments arranged by size:
467 94 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 220 627
Size: 97 123 407
Fragments arranged by size:
407 123 97
BsiHKAI G_wGCw'C
Cuts at: 0 224 552 627
Size: 224 328 75
Fragments arranged by size:
328 224 75
BsmI GAATG_Cn'
Cuts at: 0 457 627
Size: 457 170
Bsp1286I G_dGCh'C
Cuts at: 0 7 224 544 552 627
Size: 7 217 320 8 75
Fragments arranged by size:
320 217 75 8 7
BspEI T'CCGG_A
Cuts at: 0 251 627
Size: 251 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 19 627
Size: 19 608
BstXI CCAn_nnnn'nTGG
Cuts at: 0 309 627
Size: 309 318
BtsI GCAGTG_nn'
Cuts at: 0 281 627
Size: 281 346
EaeI y'GGCC_r
Cuts at: 0 367 627
Size: 367 260
EarI CTCTTCn'nnn_
Cuts at: 0 47 627
Size: 47 580
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 627
Size: 109 518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 627
Size: 547 80
EcoO109I rG'GnC_Cy
Cuts at: 0 540 541 627
Size: 540 1 86
Fragments arranged by size:
540 86 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 501 627
Size: 501 126
FspI TGC'GCA
Cuts at: 0 148 627
Size: 148 479
FspAI rTGC'GCAy
Cuts at: 0 148 627
Size: 148 479
HaeII r_GCGC'y
Cuts at: 0 61 73 627
Size: 61 12 554
Fragments arranged by size:
554 61 12
KasI G'GCGC_C
Cuts at: 0 57 69 627
Size: 57 12 558
Fragments arranged by size:
558 57 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 627
Size: 38 589
MspA1I CmG'CkG
Cuts at: 0 108 525 627
Size: 108 417 102
Fragments arranged by size:
417 108 102
NarI GG'CG_CC
Cuts at: 0 58 70 627
Size: 58 12 557
Fragments arranged by size:
557 58 12
NdeI CA'TA_TG
Cuts at: 0 506 627
Size: 506 121
NspI r_CATG'y
Cuts at: 0 387 627
Size: 387 240
PfoI T'CCnGG_A
Cuts at: 0 611 627
Size: 611 16
PspOMI G'GGCC_C
Cuts at: 0 540 627
Size: 540 87
PvuII CAG'CTG
Cuts at: 0 108 627
Size: 108 519
SexAI A'CCwGG_T
Cuts at: 0 333 627
Size: 333 294
SfoI GGC'GCC
Cuts at: 0 59 71 627
Size: 59 12 556
Fragments arranged by size:
556 59 12
SmaI CCC'GGG
Cuts at: 0 88 627
Size: 88 539
SphI G_CATG'C
Cuts at: 0 387 627
Size: 387 240
SrfI GCCC'GGGC
Cuts at: 0 88 627
Size: 88 539
StyI C'CwwG_G
Cuts at: 0 41 302 446 627
Size: 41 261 144 181
Fragments arranged by size:
261 181 144 41
XbaI T'CTAG_A
Cuts at: 0 432 627
Size: 432 195
XmaI C'CCGG_G
Cuts at: 0 86 627
Size: 86 541
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbvCI BcgI BglI Bme1580I BmrI Bpu10I
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BstXI BtsI EaeI EarI EciI Eco57MI
EcoO109I FalI FspI FspAI HaeII KasI MmeI MspA1I
NarI NdeI NspI PfoI PspOMI PvuII SexAI SfoI
SmaI SphI SrfI StyI XbaI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaXI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FseI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MscI MslI NaeI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmlI SnaBI SpeI SspI StuI SwaI TaqII TatI
TstI Tth111I XcmI XhoI XmnI ZraI