Restriction map for
pF1KB0972
(Linear) (Six-base) MAPSORT of: KIBB0972.seq Check: 9332 from: 1 to: 1311
KIBB0972.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 586 854 1161 1311
Size: 586 268 307 150
Fragments arranged by size:
586 307 268 150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1311
Size: 1007 304
AflIII A'CryG_T
Cuts at: 0 20 1311
Size: 20 1291
AhdI GACnn_n'nnGTC
Cuts at: 0 954 1182 1311
Size: 954 228 129
Fragments arranged by size:
954 228 129
AlwNI CAG_nnn'CTG
Cuts at: 0 335 807 1044 1233 1311
Size: 335 472 237 189 78
Fragments arranged by size:
472 335 237 189 78
ApoI r'AATT_y
Cuts at: 0 142 154 410 874 1311
Size: 142 12 256 464 437
Fragments arranged by size:
464 437 256 142 12
BamHI G'GATC_C
Cuts at: 0 1206 1311
Size: 1206 105
BanII G_rGCy'C
Cuts at: 0 81 821 1311
Size: 81 740 490
Fragments arranged by size:
740 490 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 445 1119 1311
Size: 445 674 192
Fragments arranged by size:
674 445 192
BbvCI CC'TCA_GC
Cuts at: 0 1054 1311
Size: 1054 257
BciVI GTATCCnnnnn_n'
Cuts at: 0 35 1311
Size: 35 1276
BglI GCCn_nnn'nGGC
Cuts at: 0 49 1311
Size: 49 1262
BglII A'GATC_T
Cuts at: 0 1301 1311
Size: 1301 10
BlpI GC'TnA_GC
Cuts at: 0 737 815 1311
Size: 737 78 496
Fragments arranged by size:
737 496 78
Bme1580I G_kGCm'C
Cuts at: 0 323 1311
Size: 323 988
BmrI ACTGGGnnnn_n'
Cuts at: 0 588 636 1311
Size: 588 48 675
Fragments arranged by size:
675 588 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 248 1136 1311
Size: 57 191 888 175
Fragments arranged by size:
888 191 175 57
Bpu10I CC'TnA_GC
Cuts at: 0 80 1054 1311
Size: 80 974 257
Fragments arranged by size:
974 257 80
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 1216 1311
Size: 661 555 95
Fragments arranged by size:
661 555 95
BsaAI yAC'GTr
Cuts at: 0 23 1311
Size: 23 1288
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1109 1139 1311
Size: 1109 30 172
Fragments arranged by size:
1109 172 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 763 793 796 1234 1311
Size: 259 504 30 3 438 77
Fragments arranged by size:
504 438 259 77 30 3
BseYI C'CCAG_C
Cuts at: 0 7 174 324 1311
Size: 7 167 150 987
Fragments arranged by size:
987 167 150 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 480 1311
Size: 307 173 831
Fragments arranged by size:
831 307 173
BsiHKAI G_wGCw'C
Cuts at: 0 95 938 1311
Size: 95 843 373
Fragments arranged by size:
843 373 95
BsmI GAATG_Cn'
Cuts at: 0 393 1068 1311
Size: 393 675 243
Fragments arranged by size:
675 393 243
Bsp1286I G_dGCh'C
Cuts at: 0 81 95 323 821 938 1311
Size: 81 14 228 498 117 373
Fragments arranged by size:
498 373 228 117 81 14
BspHI T'CATG_A
Cuts at: 0 1007 1311
Size: 1007 304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 510 586 854 1161 1311
Size: 510 76 268 307 150
Fragments arranged by size:
510 307 268 150 76
BsrBI CCG'CTC
Cuts at: 0 741 1311
Size: 741 570
BsrDI GCAATG_nn'
Cuts at: 0 221 246 1311
Size: 221 25 1065
Fragments arranged by size:
1065 221 25
BsrFI r'CCGG_y
Cuts at: 0 163 1311
Size: 163 1148
BsrGI T'GTAC_A
Cuts at: 0 17 1311
Size: 17 1294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 929 1311
Size: 929 382
BstYI r'GATC_y
Cuts at: 0 1206 1301 1311
Size: 1206 95 10
Fragments arranged by size:
1206 95 10
Bsu36I CC'TnA_GG
Cuts at: 0 776 1311
Size: 776 535
BtgI C'CryG_G
Cuts at: 0 691 1311
Size: 691 620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 42 1311
Size: 42 1269
BtsI GCAGTG_nn'
Cuts at: 0 295 916 1311
Size: 295 621 395
Fragments arranged by size:
621 395 295
EaeI y'GGCC_r
Cuts at: 0 120 1311
Size: 120 1191
EarI CTCTTCn'nnn_
Cuts at: 0 710 1311
Size: 710 601
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 529 1212 1311
Size: 529 683 99
Fragments arranged by size:
683 529 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 248 1007 1136 1311
Size: 57 191 759 129 175
Fragments arranged by size:
759 191 175 129 57
EcoO109I rG'GnC_Cy
Cuts at: 0 11 41 171 831 1262 1311
Size: 11 30 130 660 431 49
Fragments arranged by size:
660 431 130 49 30 11
EcoRI G'AATT_C
Cuts at: 0 410 1311
Size: 410 901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 852 876 1083 1311
Size: 852 24 207 228
Fragments arranged by size:
852 228 207 24
HaeII r_GCGC'y
Cuts at: 0 808 1311
Size: 808 503
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 940 972 1002 1034 1109 1141
Size: 892 32 16 32 30 32 75 32
Cuts at: 1141 1168 1200 1311
Size: 27 32 111
Fragments arranged by size:
892 111 75 32 32 32 32 32
30 27 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1311
Size: 848 463
MscI TGG'CCA
Cuts at: 0 122 1311
Size: 122 1189
MslI CAynn'nnrTG
Cuts at: 0 1030 1191 1311
Size: 1030 161 120
Fragments arranged by size:
1030 161 120
MspA1I CmG'CkG
Cuts at: 0 74 566 1155 1311
Size: 74 492 589 156
Fragments arranged by size:
589 492 156 74
NaeI GCC'GGC
Cuts at: 0 165 1311
Size: 165 1146
NgoMIV G'CCGG_C
Cuts at: 0 163 1311
Size: 163 1148
NspI r_CATG'y
Cuts at: 0 1276 1311
Size: 1276 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 335 566 1044 1233 1275 1311
Size: 335 231 478 189 42 36
Fragments arranged by size:
478 335 231 189 42 36
PpuMI rG'GwC_Cy
Cuts at: 0 11 171 831 1311
Size: 11 160 660 480
Fragments arranged by size:
660 480 160 11
SanDI GG'GwC_CC
Cuts at: 0 11 1311
Size: 11 1300
ScaI AGT'ACT
Cuts at: 0 261 889 1246 1311
Size: 261 628 357 65
Fragments arranged by size:
628 357 261 65
StuI AGG'CCT
Cuts at: 0 547 1311
Size: 547 764
StyI C'CwwG_G
Cuts at: 0 350 821 827 1311
Size: 350 471 6 484
Fragments arranged by size:
484 471 350 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 327 1311
Size: 282 45 984
Fragments arranged by size:
984 282 45
TatI w'GTAC_w
Cuts at: 0 17 68 259 887 1244 1311
Size: 17 51 191 628 357 67
Fragments arranged by size:
628 357 191 67 51 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 188 220 1311
Size: 95 32 61 32 1091
Fragments arranged by size:
1091 95 61 32 32
XbaI T'CTAG_A
Cuts at: 0 525 1311
Size: 525 786
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 115 332 1311
Size: 115 217 979
Fragments arranged by size:
979 217 115
XmnI GAAnn'nnTTC
Cuts at: 0 1066 1311
Size: 1066 245
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApoI BamHI BanII
BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NgoMIV NspI PflMI
PpuMI SanDI ScaI StuI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BcgI BclI BfrBI BmgBI
BmtI BplI BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BssHII BssSI BstAPI BstBI BstEII BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XhoI XmaI
ZraI