(Linear) (Six-base) MAPSORT of: KIBB0977.seq Check: 5267 from: 1 to: 564 KIBB0977.orf With 182 enzymes: SgfI * October 7, 2009 14:15 .. AvaI C'yCGr_G Cuts at: 0 337 564 Size: 337 227 AvrII C'CTAG_G Cuts at: 0 31 564 Size: 31 533 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 64 98 564 Size: 64 34 466 Fragments arranged by size: 466 64 34 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 564 Size: 295 269 BsaI GGTCTCn'nnnn_ Cuts at: 0 21 564 Size: 21 543 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2 564 Size: 2 562 Bsu36I CC'TnA_GG Cuts at: 0 423 548 564 Size: 423 125 16 Fragments arranged by size: 423 125 16 BtgI C'CryG_G Cuts at: 0 267 564 Size: 267 297 DraIII CAC_nnn'GTG Cuts at: 0 501 564 Size: 501 63 EarI CTCTTCn'nnn_ Cuts at: 0 148 527 564 Size: 148 379 37 Fragments arranged by size: 379 148 37 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 564 Size: 295 269 HincII GTy'rAC Cuts at: 0 453 564 Size: 453 111 HpaI GTT'AAC Cuts at: 0 453 564 Size: 453 111 MslI CAynn'nnrTG Cuts at: 0 142 564 Size: 142 422 MspA1I CmG'CkG Cuts at: 0 414 564 Size: 414 150 NspI r_CATG'y Cuts at: 0 487 564 Size: 487 77 PvuII CAG'CTG Cuts at: 0 414 564 Size: 414 150 StuI AGG'CCT Cuts at: 0 70 564 Size: 70 494 StyI C'CwwG_G Cuts at: 0 31 564 Size: 31 533 XmnI GAAnn'nnTTC Cuts at: 0 363 564 Size: 363 201 Enzymes that do cut: AvaI AvrII BcgI BpmI BsaI BsmBI Bsu36I BtgI DraIII EarI Eco57MI HincII HpaI MslI MspA1I NspI PvuII StuI StyI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HindIII KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI