(Linear) (Six-base) MAPSORT of: KIBB0979.seq Check: 5926 from: 1 to: 690
KIBB0979.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 647 690
Size: 392 255 43
Fragments arranged by size:
392 255 43
AflIII A'CryG_T
Cuts at: 0 66 690
Size: 66 624
ApoI r'AATT_y
Cuts at: 0 11 109 294 690
Size: 11 98 185 396
Fragments arranged by size:
396 185 98 11
BanII G_rGCy'C
Cuts at: 0 364 690
Size: 364 326
BglI GCCn_nnn'nGGC
Cuts at: 0 684 690
Size: 684 6
BlpI GC'TnA_GC
Cuts at: 0 128 690
Size: 128 562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 108 690
Size: 108 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 690
Size: 608 82
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 472 690
Size: 469 3 218
Fragments arranged by size:
469 218 3
BsiHKAI G_wGCw'C
Cuts at: 0 364 510 690
Size: 364 146 180
Fragments arranged by size:
364 180 146
Bsp1286I G_dGCh'C
Cuts at: 0 364 510 690
Size: 364 146 180
Fragments arranged by size:
364 180 146
BspHI T'CATG_A
Cuts at: 0 290 690
Size: 290 400
BtsI GCAGTG_nn'
Cuts at: 0 270 690
Size: 270 420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 246 281 690
Size: 246 35 409
Fragments arranged by size:
409 246 35
EaeI y'GGCC_r
Cuts at: 0 162 690
Size: 162 528
EcoICRI GAG'CTC
Cuts at: 0 362 690
Size: 362 328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 608 647 690
Size: 392 216 39 43
Fragments arranged by size:
392 216 43 39
EcoRV GAT'ATC
Cuts at: 0 327 690
Size: 327 363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 130 690
Size: 130 560
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 690
Size: 373 32 285
Fragments arranged by size:
373 285 32
MscI TGG'CCA
Cuts at: 0 164 690
Size: 164 526
MspA1I CmG'CkG
Cuts at: 0 444 690
Size: 444 246
NspI r_CATG'y
Cuts at: 0 70 690
Size: 70 620
PciI A'CATG_T
Cuts at: 0 66 690
Size: 66 624
PsiI TTA'TAA
Cuts at: 0 320 690
Size: 320 370
PvuII CAG'CTG
Cuts at: 0 444 690
Size: 444 246
SacI G_AGCT'C
Cuts at: 0 364 690
Size: 364 326
SfcI C'TryA_G
Cuts at: 0 577 690
Size: 577 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 594 636 690
Size: 463 131 42 54
Fragments arranged by size:
463 131 54 42
XmnI GAAnn'nnTTC
Cuts at: 0 110 690
Size: 110 580
Enzymes that do cut:
AcuI AflIII ApoI BanII BglI BlpI BplI BpmI
BseRI BsiHKAI Bsp1286I BspHI BtsI CspCI EaeI EcoICRI
Eco57MI EcoRV FalI Hin4I MscI MspA1I NspI PciI
PsiI PvuII SacI SfcI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I
BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI DraI DraIII DrdI EagI EarI
EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI