Restriction map for
pF1KB0982
(Linear) (Six-base) MAPSORT of: KIBB0982.seq Check: 9696 from: 1 to: 1341
KIBB0982.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 572 1065 1341
Size: 572 493 276
Fragments arranged by size:
572 493 276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 911 1140 1341
Size: 806 105 229 201
Fragments arranged by size:
806 229 201 105
AfeI AGC'GCT
Cuts at: 0 1093 1341
Size: 1093 248
AleI CACnn'nnGTG
Cuts at: 0 1102 1341
Size: 1102 239
AlwNI CAG_nnn'CTG
Cuts at: 0 144 1341
Size: 144 1197
ApaI G_GGCC'C
Cuts at: 0 466 1341
Size: 466 875
ApaLI G'TGCA_C
Cuts at: 0 301 1341
Size: 301 1040
AvaI C'yCGr_G
Cuts at: 0 800 989 1082 1341
Size: 800 189 93 259
Fragments arranged by size:
800 259 189 93
BamHI G'GATC_C
Cuts at: 0 1307 1341
Size: 1307 34
BanI G'GyrC_C
Cuts at: 0 479 1197 1341
Size: 479 718 144
Fragments arranged by size:
718 479 144
BanII G_rGCy'C
Cuts at: 0 466 988 1341
Size: 466 522 353
Fragments arranged by size:
522 466 353
BbeI G_GCGC'C
Cuts at: 0 483 1341
Size: 483 858
BbvCI CC'TCA_GC
Cuts at: 0 690 951 1341
Size: 690 261 390
Fragments arranged by size:
690 390 261
BglI GCCn_nnn'nGGC
Cuts at: 0 37 46 477 718 1341
Size: 37 9 431 241 623
Fragments arranged by size:
623 431 241 37 9
Bme1580I G_kGCm'C
Cuts at: 0 305 466 1202 1341
Size: 305 161 736 139
Fragments arranged by size:
736 305 161 139
BmgBI CAC'GTC
Cuts at: 0 276 1341
Size: 276 1065
BmrI ACTGGGnnnn_n'
Cuts at: 0 342 480 767 803 809 1341
Size: 342 138 287 36 6 532
Fragments arranged by size:
532 342 287 138 36 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 1341
Size: 378 963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 510 860 1341
Size: 373 137 350 481
Fragments arranged by size:
481 373 350 137
Bpu10I CC'TnA_GC
Cuts at: 0 690 951 1341
Size: 690 261 390
Fragments arranged by size:
690 390 261
BsaI GGTCTCn'nnnn_
Cuts at: 0 772 1341
Size: 772 569
BsaAI yAC'GTr
Cuts at: 0 248 1009 1341
Size: 248 761 332
Fragments arranged by size:
761 332 248
BsaHI Gr'CG_yC
Cuts at: 0 480 1062 1341
Size: 480 582 279
Fragments arranged by size:
582 480 279
BsaWI w'CCGG_w
Cuts at: 0 1301 1341
Size: 1301 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 496 526 901 931 1341
Size: 4 30 462 30 375 30 410
Fragments arranged by size:
462 410 375 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 910 913 916 919 922 1341
Size: 784 126 3 3 3 3 419
Fragments arranged by size:
784 419 126 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 222 467 1341
Size: 222 245 874
Fragments arranged by size:
874 245 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 270 1341
Size: 46 224 1071
Fragments arranged by size:
1071 224 46
BsiHKAI G_wGCw'C
Cuts at: 0 281 305 1022 1136 1341
Size: 281 24 717 114 205
Fragments arranged by size:
717 281 205 114 24
Bsp1286I G_dGCh'C
Cuts at: 0 281 305 466 988 1022 1136 1202 1341
Size: 281 24 161 522 34 114 66 139
Fragments arranged by size:
522 281 161 139 114 66 34 24
BspEI T'CCGG_A
Cuts at: 0 1301 1341
Size: 1301 40
BspHI T'CATG_A
Cuts at: 0 560 1341
Size: 560 781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 584 1341
Size: 584 757
BsrBI CCG'CTC
Cuts at: 0 122 1341
Size: 122 1219
BssHII G'CGCG_C
Cuts at: 0 129 347 1341
Size: 129 218 994
Fragments arranged by size:
994 218 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 251 389 1341
Size: 251 138 952
Fragments arranged by size:
952 251 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 818 1341
Size: 321 497 523
Fragments arranged by size:
523 497 321
BstYI r'GATC_y
Cuts at: 0 535 1307 1341
Size: 535 772 34
Fragments arranged by size:
772 535 34
BtgI C'CryG_G
Cuts at: 0 672 1203 1321 1341
Size: 672 531 118 20
Fragments arranged by size:
672 531 118 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 399 1341
Size: 399 942
BtsI GCAGTG_nn'
Cuts at: 0 938 1060 1188 1341
Size: 938 122 128 153
Fragments arranged by size:
938 153 128 122
DraIII CAC_nnn'GTG
Cuts at: 0 251 407 1341
Size: 251 156 934
Fragments arranged by size:
934 251 156
EaeI y'GGCC_r
Cuts at: 0 38 50 161 182 209 998 1011 1341
Size: 38 12 111 21 27 789 13 330
Fragments arranged by size:
789 330 111 38 27 21 13 12
EarI CTCTTCn'nnn_
Cuts at: 0 286 887 908 1216 1341
Size: 286 601 21 308 125
Fragments arranged by size:
601 308 286 125 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1279 1341
Size: 1279 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 510 806 860 911 1140 1341
Size: 373 137 296 54 51 229 201
Fragments arranged by size:
373 296 229 201 137 54 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1302 1341
Size: 1302 39
EcoO109I rG'GnC_Cy
Cuts at: 0 12 462 463 616 634 797 1341
Size: 12 450 1 153 18 163 544
Fragments arranged by size:
544 450 163 153 18 12 1
FspI TGC'GCA
Cuts at: 0 580 1341
Size: 580 761
HaeII r_GCGC'y
Cuts at: 0 271 483 1095 1341
Size: 271 212 612 246
Fragments arranged by size:
612 271 246 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 1341
Size: 4 32 1305
Fragments arranged by size:
1305 32 4
HincII GTy'rAC
Cuts at: 0 113 738 1066 1341
Size: 113 625 328 275
Fragments arranged by size:
625 328 275 113
KasI G'GCGC_C
Cuts at: 0 479 1341
Size: 479 862
MscI TGG'CCA
Cuts at: 0 184 211 1000 1013 1341
Size: 184 27 789 13 328
Fragments arranged by size:
789 328 184 27 13
MslI CAynn'nnrTG
Cuts at: 0 178 1102 1341
Size: 178 924 239
Fragments arranged by size:
924 239 178
MspA1I CmG'CkG
Cuts at: 0 37 969 1077 1341
Size: 37 932 108 264
Fragments arranged by size:
932 264 108 37
NarI GG'CG_CC
Cuts at: 0 480 1341
Size: 480 861
NcoI C'CATG_G
Cuts at: 0 672 1203 1341
Size: 672 531 138
Fragments arranged by size:
672 531 138
PasI CC'CwG_GG
Cuts at: 0 620 1341
Size: 620 721
PmlI CAC'GTG
Cuts at: 0 248 1341
Size: 248 1093
PpuMI rG'GwC_Cy
Cuts at: 0 12 616 797 1341
Size: 12 604 181 544
Fragments arranged by size:
604 544 181 12
PspOMI G'GGCC_C
Cuts at: 0 462 1341
Size: 462 879
PstI C_TGCA'G
Cuts at: 0 974 1341
Size: 974 367
PvuII CAG'CTG
Cuts at: 0 1077 1341
Size: 1077 264
SalI G'TCGA_C
Cuts at: 0 1064 1341
Size: 1064 277
SanDI GG'GwC_CC
Cuts at: 0 797 1341
Size: 797 544
SapI GCTCTTCn'nnn_
Cuts at: 0 286 1341
Size: 286 1055
SfcI C'TryA_G
Cuts at: 0 970 1043 1161 1341
Size: 970 73 118 180
Fragments arranged by size:
970 180 118 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 37 1341
Size: 37 1304
SfoI GGC'GCC
Cuts at: 0 481 1341
Size: 481 860
SmaI CCC'GGG
Cuts at: 0 802 991 1084 1341
Size: 802 189 93 257
Fragments arranged by size:
802 257 189 93
StuI AGG'CCT
Cuts at: 0 508 1341
Size: 508 833
StyI C'CwwG_G
Cuts at: 0 626 672 1203 1341
Size: 626 46 531 138
Fragments arranged by size:
626 531 138 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 828 1027 1341
Size: 828 199 314
Fragments arranged by size:
828 314 199
TatI w'GTAC_w
Cuts at: 0 356 1341
Size: 356 985
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 901 933 1341
Size: 736 32 133 32 408
Fragments arranged by size:
736 408 133 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1341
Size: 219 1122
XmaI C'CCGG_G
Cuts at: 0 800 989 1082 1341
Size: 800 189 93 259
Fragments arranged by size:
800 259 189 93
XmnI GAAnn'nnTTC
Cuts at: 0 145 1093 1341
Size: 145 948 248
Fragments arranged by size:
948 248 145
Enzymes that do cut:
AccI AcuI AfeI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbvCI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BssHII BstAPI BstXI BstYI BtgI BtgZI
BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII KasI MscI MslI MspA1I
NarI NcoI PasI PmlI PpuMI PspOMI PstI PvuII
SalI SanDI SapI SfcI SfiI SfoI SmaI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BpuEI BsaBI BsiEI BsiWI BsmI BsmBI BsrDI BsrFI
BsrGI BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SbfI ScaI SexAI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI