Restriction map for
pF1KB0987
(Linear) (Six-base) MAPSORT of: KIBB0987.seq Check: 4308 from: 1 to: 774
KIBB0987.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 449 774
Size: 449 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 379 682 739 774
Size: 82 297 303 57 35
Fragments arranged by size:
303 297 82 57 35
AflIII A'CryG_T
Cuts at: 0 296 774
Size: 296 478
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 162 194 774
Size: 162 32 580
Fragments arranged by size:
580 162 32
AlwNI CAG_nnn'CTG
Cuts at: 0 142 242 435 534 774
Size: 142 100 193 99 240
Fragments arranged by size:
240 193 142 100 99
BanI G'GyrC_C
Cuts at: 0 514 774
Size: 514 260
BbsI GAAGACnn'nnnn_
Cuts at: 0 141 774
Size: 141 633
BbvCI CC'TCA_GC
Cuts at: 0 135 331 524 774
Size: 135 196 193 250
Fragments arranged by size:
250 196 193 135
BlpI GC'TnA_GC
Cuts at: 0 410 774
Size: 410 364
Bme1580I G_kGCm'C
Cuts at: 0 519 774
Size: 519 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 717 774
Size: 717 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 774
Size: 150 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 565 570 774
Size: 490 75 5 204
Fragments arranged by size:
490 204 75 5
Bpu10I CC'TnA_GC
Cuts at: 0 135 331 524 774
Size: 135 196 193 250
Fragments arranged by size:
250 196 193 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 774
Size: 388 386
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 199 262 774
Size: 43 156 63 512
Fragments arranged by size:
512 156 63 43
BseYI C'CCAG_C
Cuts at: 0 475 510 534 774
Size: 475 35 24 240
Fragments arranged by size:
475 240 35 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 774
Size: 255 519
BsiHKAI G_wGCw'C
Cuts at: 0 79 551 696 774
Size: 79 472 145 78
Fragments arranged by size:
472 145 79 78
BsmI GAATG_Cn'
Cuts at: 0 280 672 774
Size: 280 392 102
Fragments arranged by size:
392 280 102
Bsp1286I G_dGCh'C
Cuts at: 0 79 519 551 696 774
Size: 79 440 32 145 78
Fragments arranged by size:
440 145 79 78 32
BspHI T'CATG_A
Cuts at: 0 306 774
Size: 306 468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 774
Size: 227 547
BsrDI GCAATG_nn'
Cuts at: 0 383 774
Size: 383 391
BstAPI GCAn_nnn'nTGC
Cuts at: 0 83 435 774
Size: 83 352 339
Fragments arranged by size:
352 339 83
BtgI C'CryG_G
Cuts at: 0 453 774
Size: 453 321
BtsI GCAGTG_nn'
Cuts at: 0 535 774
Size: 535 239
EaeI y'GGCC_r
Cuts at: 0 566 635 774
Size: 566 69 139
Fragments arranged by size:
566 139 69
EarI CTCTTCn'nnn_
Cuts at: 0 100 220 774
Size: 100 120 554
Fragments arranged by size:
554 120 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 379 490 565 570 682 739 774
Size: 82 297 111 75 5 112 57 35
Fragments arranged by size:
297 112 111 82 75 57 35 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 774
Size: 64 710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 774
Size: 707 67
MspA1I CmG'CkG
Cuts at: 0 399 510 543 774
Size: 399 111 33 231
Fragments arranged by size:
399 231 111 33
NspI r_CATG'y
Cuts at: 0 124 300 555 774
Size: 124 176 255 219
Fragments arranged by size:
255 219 176 124
PciI A'CATG_T
Cuts at: 0 296 774
Size: 296 478
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 774
Size: 468 306
PstI C_TGCA'G
Cuts at: 0 431 440 774
Size: 431 9 334
Fragments arranged by size:
431 334 9
PvuII CAG'CTG
Cuts at: 0 510 543 774
Size: 510 33 231
Fragments arranged by size:
510 231 33
SfcI C'TryA_G
Cuts at: 0 427 436 774
Size: 427 9 338
Fragments arranged by size:
427 338 9
SmlI C'TyrA_G
Cuts at: 0 403 774
Size: 403 371
StuI AGG'CCT
Cuts at: 0 133 774
Size: 133 641
StyI C'CwwG_G
Cuts at: 0 638 774
Size: 638 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 774
Size: 502 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 507 774
Size: 507 267
XmnI GAAnn'nnTTC
Cuts at: 0 749 774
Size: 749 25
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI BanI BbsI BbvCI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstAPI BtgI BtsI EaeI EarI Eco57MI EcoNI MmeI
MspA1I NspI PciI PflMI PstI PvuII SfcI SmlI
StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmaI ZraI