Restriction map for
pF1KB0993
(Linear) (Six-base) MAPSORT of: KIBB0993.seq Check: 4755 from: 1 to: 834
KIBB0993.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 616 834
Size: 196 420 218
Fragments arranged by size:
420 218 196
ApaI G_GGCC'C
Cuts at: 0 94 834
Size: 94 740
ApoI r'AATT_y
Cuts at: 0 571 834
Size: 571 263
AvaI C'yCGr_G
Cuts at: 0 93 772 834
Size: 93 679 62
Fragments arranged by size:
679 93 62
BanII G_rGCy'C
Cuts at: 0 20 94 833 834
Size: 20 74 739 1
Fragments arranged by size:
739 74 20 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 25 566 834
Size: 25 541 268
Fragments arranged by size:
541 268 25
BbvCI CC'TCA_GC
Cuts at: 0 110 834
Size: 110 724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 448 482 834
Size: 448 34 352
Fragments arranged by size:
448 352 34
Bme1580I G_kGCm'C
Cuts at: 0 94 834
Size: 94 740
BmrI ACTGGGnnnn_n'
Cuts at: 0 132 834
Size: 132 702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 834
Size: 275 559
Bpu10I CC'TnA_GC
Cuts at: 0 14 110 834
Size: 14 96 724
Fragments arranged by size:
724 96 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 775 834
Size: 469 306 59
Fragments arranged by size:
469 306 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 376 755 834
Size: 295 81 379 79
Fragments arranged by size:
379 295 81 79
BsaAI yAC'GTr
Cuts at: 0 591 834
Size: 591 243
BsaBI GATnn'nnATC
Cuts at: 0 436 834
Size: 436 398
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 834
Size: 163 30 641
Fragments arranged by size:
641 163 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 535 790 834
Size: 127 408 255 44
Fragments arranged by size:
408 255 127 44
BseYI C'CCAG_C
Cuts at: 0 87 136 744 766 834
Size: 87 49 608 22 68
Fragments arranged by size:
608 87 68 49 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 834
Size: 319 515
BsiHKAI G_wGCw'C
Cuts at: 0 833 834
Size: 833 1
Bsp1286I G_dGCh'C
Cuts at: 0 20 94 833 834
Size: 20 74 739 1
Fragments arranged by size:
739 74 20 1
BsrFI r'CCGG_y
Cuts at: 0 75 196 349 834
Size: 75 121 153 485
Fragments arranged by size:
485 153 121 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 834
Size: 618 216
BtsI GCAGTG_nn'
Cuts at: 0 499 634 834
Size: 499 135 200
Fragments arranged by size:
499 200 135
ClaI AT'CG_AT
Cuts at: 0 360 834
Size: 360 474
EarI CTCTTCn'nnn_
Cuts at: 0 506 679 834
Size: 506 173 155
Fragments arranged by size:
506 173 155
EcoICRI GAG'CTC
Cuts at: 0 831 834
Size: 831 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 275 616 834
Size: 196 79 341 218
Fragments arranged by size:
341 218 196 79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 834
Size: 337 497
EcoRI G'AATT_C
Cuts at: 0 571 834
Size: 571 263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 201 294 834
Size: 201 93 540
Fragments arranged by size:
540 201 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 163 195 616 648 834
Size: 7 32 124 32 421 32 186
Fragments arranged by size:
421 186 124 32 32 32 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 834
Size: 254 580
MspA1I CmG'CkG
Cuts at: 0 29 655 834
Size: 29 626 179
Fragments arranged by size:
626 179 29
NaeI GCC'GGC
Cuts at: 0 77 834
Size: 77 757
NgoMIV G'CCGG_C
Cuts at: 0 75 834
Size: 75 759
NspI r_CATG'y
Cuts at: 0 378 834
Size: 378 456
PasI CC'CwG_GG
Cuts at: 0 660 834
Size: 660 174
PfoI T'CCnGG_A
Cuts at: 0 236 320 834
Size: 236 84 514
Fragments arranged by size:
514 236 84
PmlI CAC'GTG
Cuts at: 0 591 834
Size: 591 243
PspOMI G'GGCC_C
Cuts at: 0 90 834
Size: 90 744
PstI C_TGCA'G
Cuts at: 0 471 834
Size: 471 363
PvuII CAG'CTG
Cuts at: 0 655 834
Size: 655 179
SacI G_AGCT'C
Cuts at: 0 833 834
Size: 833 1
SapI GCTCTTCn'nnn_
Cuts at: 0 679 834
Size: 679 155
SexAI A'CCwGG_T
Cuts at: 0 404 834
Size: 404 430
SfcI C'TryA_G
Cuts at: 0 467 834
Size: 467 367
SmlI C'TyrA_G
Cuts at: 0 484 754 834
Size: 484 270 80
Fragments arranged by size:
484 270 80
StuI AGG'CCT
Cuts at: 0 250 834
Size: 250 584
StyI C'CwwG_G
Cuts at: 0 206 368 698 834
Size: 206 162 330 136
Fragments arranged by size:
330 206 162 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 736 834
Size: 736 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 250 834
Size: 250 584
Enzymes that do cut:
AcuI ApaI ApoI AvaI BanII BbsI BbvCI BcgI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrFI BstXI
BtsI ClaI EarI EcoICRI Eco57MI EcoNI EcoRI FalI
Hin4I MmeI MspA1I NaeI NgoMIV NspI PasI PfoI
PmlI PspOMI PstI PvuII SacI SapI SexAI SfcI
SmlI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoO109I EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI