Restriction map for
pF1KB0994
(Linear) (Six-base) MAPSORT of: KIBB0994.seq Check: 7774 from: 1 to: 1383
KIBB0994.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 726 1383
Size: 726 657
AccI GT'mk_AC
Cuts at: 0 845 1383
Size: 845 538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1383
Size: 699 684
AfeI AGC'GCT
Cuts at: 0 1348 1383
Size: 1348 35
AflIII A'CryG_T
Cuts at: 0 38 204 1383
Size: 38 166 1179
Fragments arranged by size:
1179 166 38
AlwNI CAG_nnn'CTG
Cuts at: 0 247 789 1383
Size: 247 542 594
Fragments arranged by size:
594 542 247
ApaI G_GGCC'C
Cuts at: 0 514 1109 1160 1383
Size: 514 595 51 223
Fragments arranged by size:
595 514 223 51
ApaLI G'TGCA_C
Cuts at: 0 520 1383
Size: 520 863
BanI G'GyrC_C
Cuts at: 0 266 1383
Size: 266 1117
BanII G_rGCy'C
Cuts at: 0 368 514 614 1040 1109 1160 1340 1383
Size: 368 146 100 426 69 51 180 43
Fragments arranged by size:
426 368 180 146 100 69 51 43
BbvCI CC'TCA_GC
Cuts at: 0 1127 1210 1383
Size: 1127 83 173
Fragments arranged by size:
1127 173 83
BclI T'GATC_A
Cuts at: 0 464 1383
Size: 464 919
BglI GCCn_nnn'nGGC
Cuts at: 0 1123 1383
Size: 1123 260
Bme1580I G_kGCm'C
Cuts at: 0 271 487 514 524 1109 1160 1383
Size: 271 216 27 10 585 51 223
Fragments arranged by size:
585 271 223 216 51 27 10
BmgBI CAC'GTC
Cuts at: 0 205 480 1383
Size: 205 275 903
Fragments arranged by size:
903 275 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 497 517 1383
Size: 497 20 866
Fragments arranged by size:
866 497 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 400 570 784 1000 1303 1318 1324
Size: 6 394 170 214 216 303 15 6
Cuts at: 1324 1381 1383
Size: 57 2
Fragments arranged by size:
394 303 216 214 170 57 15 6
6 2
Bpu10I CC'TnA_GC
Cuts at: 0 1127 1210 1383
Size: 1127 83 173
Fragments arranged by size:
1127 173 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1383
Size: 1159 224
BsaAI yAC'GTr
Cuts at: 0 39 1188 1383
Size: 39 1149 195
Fragments arranged by size:
1149 195 39
BsaWI w'CCGG_w
Cuts at: 0 875 1383
Size: 875 508
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 904 934 1072 1102 1383
Size: 760 30 114 30 138 30 281
Fragments arranged by size:
760 281 138 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 781 1144 1153 1315 1318 1378 1383
Size: 781 363 9 162 3 60 5
Fragments arranged by size:
781 363 162 60 9 5 3
BseYI C'CCAG_C
Cuts at: 0 944 1137 1153 1273 1383
Size: 944 193 16 120 110
Fragments arranged by size:
944 193 120 110 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 467 695 895 1003 1351 1383
Size: 294 173 228 200 108 348 32
Fragments arranged by size:
348 294 228 200 173 108 32
BsiHKAI G_wGCw'C
Cuts at: 0 524 1340 1383
Size: 524 816 43
Fragments arranged by size:
816 524 43
BsmI GAATG_Cn'
Cuts at: 0 222 1383
Size: 222 1161
BsmBI CGTCTCn'nnnn_
Cuts at: 0 93 1383
Size: 93 1290
Bsp1286I G_dGCh'C
Cuts at: 0 271 368 487 514 524 614 1040 1109
Size: 271 97 119 27 10 90 426 69
Cuts at: 1109 1160 1340 1383
Size: 51 180 43
Fragments arranged by size:
426 271 180 119 97 90 69 51
43 27 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 439 726 1383
Size: 439 287 657
Fragments arranged by size:
657 439 287
BsrBI CCG'CTC
Cuts at: 0 20 732 1026 1383
Size: 20 712 294 357
Fragments arranged by size:
712 357 294 20
BsrDI GCAATG_nn'
Cuts at: 0 287 1040 1383
Size: 287 753 343
Fragments arranged by size:
753 343 287
BsrFI r'CCGG_y
Cuts at: 0 5 51 1383
Size: 5 46 1332
Fragments arranged by size:
1332 46 5
BssSI C'ACGA_G
Cuts at: 0 652 1021 1383
Size: 652 369 362
Fragments arranged by size:
652 369 362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 43 399 1383
Size: 43 356 984
Fragments arranged by size:
984 356 43
BstYI r'GATC_y
Cuts at: 0 329 698 1383
Size: 329 369 685
Fragments arranged by size:
685 369 329
BtgI C'CryG_G
Cuts at: 0 224 707 1325 1383
Size: 224 483 618 58
Fragments arranged by size:
618 483 224 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 243 1383
Size: 243 1140
BtsI GCAGTG_nn'
Cuts at: 0 683 1383
Size: 683 700
DrdI GACnn_nn'nnGTC
Cuts at: 0 313 1383
Size: 313 1070
EaeI y'GGCC_r
Cuts at: 0 53 234 494 1383
Size: 53 181 260 889
Fragments arranged by size:
889 260 181 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1383
Size: 22 1361
EcoICRI GAG'CTC
Cuts at: 0 1338 1383
Size: 1338 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 400 570 699 784 1000 1303 1318
Size: 6 394 170 129 85 216 303 15
Cuts at: 1318 1324 1381 1383
Size: 6 57 2
Fragments arranged by size:
394 303 216 170 129 85 57 15
6 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1075 1383
Size: 1075 308
EcoO109I rG'GnC_Cy
Cuts at: 0 170 342 659 900 1083 1105 1157 1224
Size: 170 172 317 241 183 22 52 67
Cuts at: 1224 1383
Size: 159
Fragments arranged by size:
317 241 183 172 170 159 67 52
22
FspI TGC'GCA
Cuts at: 0 601 1383
Size: 601 782
FspAI rTGC'GCAy
Cuts at: 0 601 1383
Size: 601 782
HaeII r_GCGC'y
Cuts at: 0 745 1350 1383
Size: 745 605 33
Fragments arranged by size:
745 605 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1041 1073 1273 1305 1383
Size: 760 32 249 32 200 32 78
Fragments arranged by size:
760 249 200 78 32 32 32
HincII GTy'rAC
Cuts at: 0 126 1218 1383
Size: 126 1092 165
Fragments arranged by size:
1092 165 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1046 1383
Size: 668 378 337
Fragments arranged by size:
668 378 337
MscI TGG'CCA
Cuts at: 0 236 1383
Size: 236 1147
MslI CAynn'nnrTG
Cuts at: 0 316 1045 1051 1383
Size: 316 729 6 332
Fragments arranged by size:
729 332 316 6
MspA1I CmG'CkG
Cuts at: 0 161 774 803 948 1383
Size: 161 613 29 145 435
Fragments arranged by size:
613 435 161 145 29
NaeI GCC'GGC
Cuts at: 0 7 53 1383
Size: 7 46 1330
Fragments arranged by size:
1330 46 7
NgoMIV G'CCGG_C
Cuts at: 0 5 51 1383
Size: 5 46 1332
Fragments arranged by size:
1332 46 5
NspI r_CATG'y
Cuts at: 0 966 1383
Size: 966 417
PasI CC'CwG_GG
Cuts at: 0 189 1383
Size: 189 1194
PfoI T'CCnGG_A
Cuts at: 0 1076 1383
Size: 1076 307
PmlI CAC'GTG
Cuts at: 0 39 1383
Size: 39 1344
PpuMI rG'GwC_Cy
Cuts at: 0 659 1083 1383
Size: 659 424 300
Fragments arranged by size:
659 424 300
PspOMI G'GGCC_C
Cuts at: 0 510 1105 1156 1383
Size: 510 595 51 227
Fragments arranged by size:
595 510 227 51
PstI C_TGCA'G
Cuts at: 0 785 1383
Size: 785 598
SacI G_AGCT'C
Cuts at: 0 1340 1383
Size: 1340 43
SanDI GG'GwC_CC
Cuts at: 0 659 1383
Size: 659 724
SbfI CC_TGCA'GG
Cuts at: 0 785 1383
Size: 785 598
SfcI C'TryA_G
Cuts at: 0 537 781 1383
Size: 537 244 602
Fragments arranged by size:
602 537 244
SmlI C'TyrA_G
Cuts at: 0 1174 1383
Size: 1174 209
SnaBI TAC'GTA
Cuts at: 0 1188 1383
Size: 1188 195
StuI AGG'CCT
Cuts at: 0 566 1383
Size: 566 817
StyI C'CwwG_G
Cuts at: 0 56 935 1383
Size: 56 879 448
Fragments arranged by size:
879 448 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 539 1110 1383
Size: 539 571 273
Fragments arranged by size:
571 539 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 904 936 1383
Size: 904 32 447
Fragments arranged by size:
904 447 32
Tth111I GACn'n_nGTC
Cuts at: 0 283 1383
Size: 283 1100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 975 1383
Size: 504 471 408
Fragments arranged by size:
504 471 408
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI ApaLI
BanI BanII BbvCI BclI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PasI PfoI PmlI PpuMI PspOMI PstI
SacI SanDI SbfI SfcI SmlI SnaBI StuI StyI
TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BfrBI BglII BlpI
BmtI BplI BsaI BsaBI BsaHI BsiEI BsiWI BspEI
BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII EagI EarI EcoRI EcoRV
FalI FseI HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
SspI SwaI TatI XbaI XhoI XmaI XmnI ZraI