(Linear) (Six-base) MAPSORT of: KIBB0995.seq  Check: 4565  from: 1  to: 852



KIBB0995.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     852 
   Size:        497     355
 
AflIII A'CryG_T

Cuts at:      0      91     852 
   Size:         91     761
 
ApaLI G'TGCA_C

Cuts at:      0     204     267     852 
   Size:        204      63     585

  Fragments arranged by size: 

                 585     204      63
 
ApoI r'AATT_y

Cuts at:      0     349     852 
   Size:        349     503
 
BanI G'GyrC_C

Cuts at:      0     700     852 
   Size:        700     152
 
BglII A'GATC_T

Cuts at:      0     582     852 
   Size:        582     270
 
Bme1580I G_kGCm'C

Cuts at:      0     208     271     852 
   Size:        208      63     581

  Fragments arranged by size: 

                 581     208      63
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     312     852 
   Size:        312     540
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     697     852 
   Size:        697     155
 
BsaWI w'CCGG_w

Cuts at:      0     832     852 
   Size:        832      20
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     277     307     852 
   Size:        277      30     545

  Fragments arranged by size: 

                 545     277      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     285     852 
   Size:        285     567
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     852 
   Size:         15     837
 
BsiHKAI G_wGCw'C

Cuts at:      0     208     271     295     852 
   Size:        208      63      24     557

  Fragments arranged by size: 

                 557     208      63      24
 
BsmI GAATG_Cn'

Cuts at:      0     133     748     852 
   Size:        133     615     104

  Fragments arranged by size: 

                 615     133     104
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     605     852 
   Size:        605     247
 
Bsp1286I G_dGCh'C

Cuts at:      0     208     271     295     852 
   Size:        208      63      24     557

  Fragments arranged by size: 

                 557     208      63      24
 
BspEI T'CCGG_A

Cuts at:      0     832     852 
   Size:        832      20
 
BsrFI r'CCGG_y

Cuts at:      0     176     852 
   Size:        176     676
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     212     275     635     852 
   Size:        212      63     360     217

  Fragments arranged by size: 

                 360     217     212      63
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     826     852 
   Size:        826      26
 
BstYI r'GATC_y

Cuts at:      0     159     582     852 
   Size:        159     423     270

  Fragments arranged by size: 

                 423     270     159
 
BtsI GCAGTG_nn'

Cuts at:      0     309     852 
   Size:        309     543
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     420     455     852 
   Size:        420      35     397

  Fragments arranged by size: 

                 420     397      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     275     852 
   Size:        275     577
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     852 
   Size:        497     355
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     828     852 
   Size:        828      24
 
HaeII r_GCGC'y

Cuts at:      0     261     852 
   Size:        261     591
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     852 
   Size:         26     826
 
MfeI C'AATT_G

Cuts at:      0     140     852 
   Size:        140     712
 
MslI CAynn'nnrTG

Cuts at:      0     185     650     852 
   Size:        185     465     202

  Fragments arranged by size: 

                 465     202     185
 
NspI r_CATG'y

Cuts at:      0      95     852 
   Size:         95     757
 
PciI A'CATG_T

Cuts at:      0      91     852 
   Size:         91     761
 
SfcI C'TryA_G

Cuts at:      0     407     573     852 
   Size:        407     166     279

  Fragments arranged by size: 

                 407     279     166
 
StyI C'CwwG_G

Cuts at:      0     248     773     852 
   Size:        248     525      79

  Fragments arranged by size: 

                 525     248      79
 
TatI w'GTAC_w

Cuts at:      0      60     852 
   Size:         60     792
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI     BanI    BglII Bme1580I     BplI
     BsaI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BsrFI   BstAPI    BstXI    BstYI     BtsI    CspCI
   DraIII  Eco57MI     FalI    HaeII    Hin4I     MfeI     MslI     NspI
     PciI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BseYI    BsiEI    BsiWI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI