Restriction map for
pF1KB0997
(Linear) (Six-base) MAPSORT of: KIBB0997.seq Check: 4633 from: 1 to: 819
KIBB0997.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 819
Size: 434 385
ApoI r'AATT_y
Cuts at: 0 185 819
Size: 185 634
AvaI C'yCGr_G
Cuts at: 0 52 76 375 819
Size: 52 24 299 444
Fragments arranged by size:
444 299 52 24
BanI G'GyrC_C
Cuts at: 0 801 819
Size: 801 18
BanII G_rGCy'C
Cuts at: 0 52 548 819
Size: 52 496 271
Fragments arranged by size:
496 271 52
BglII A'GATC_T
Cuts at: 0 774 819
Size: 774 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 511 819
Size: 511 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 755 819
Size: 312 443 64
Fragments arranged by size:
443 312 64
BsaWI w'CCGG_w
Cuts at: 0 230 819
Size: 230 589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 285 315 819
Size: 285 30 504
Fragments arranged by size:
504 285 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 756 759 819
Size: 756 3 60
Fragments arranged by size:
756 60 3
BseYI C'CCAG_C
Cuts at: 0 598 610 819
Size: 598 12 209
Fragments arranged by size:
598 209 12
BsiHKAI G_wGCw'C
Cuts at: 0 92 548 819
Size: 92 456 271
Fragments arranged by size:
456 271 92
BsmBI CGTCTCn'nnnn_
Cuts at: 0 232 819
Size: 232 587
Bsp1286I G_dGCh'C
Cuts at: 0 52 92 548 819
Size: 52 40 456 271
Fragments arranged by size:
456 271 52 40
BspEI T'CCGG_A
Cuts at: 0 230 819
Size: 230 589
BspHI T'CATG_A
Cuts at: 0 218 819
Size: 218 601
BsrDI GCAATG_nn'
Cuts at: 0 144 819
Size: 144 675
BsrFI r'CCGG_y
Cuts at: 0 780 819
Size: 780 39
BssSI C'ACGA_G
Cuts at: 0 270 819
Size: 270 549
BstYI r'GATC_y
Cuts at: 0 774 819
Size: 774 45
Bsu36I CC'TnA_GG
Cuts at: 0 605 819
Size: 605 214
BtgI C'CryG_G
Cuts at: 0 43 319 819
Size: 43 276 500
Fragments arranged by size:
500 276 43
BtsI GCAGTG_nn'
Cuts at: 0 575 819
Size: 575 244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 342 377 819
Size: 342 35 442
Fragments arranged by size:
442 342 35
EaeI y'GGCC_r
Cuts at: 0 692 819
Size: 692 127
EcoICRI GAG'CTC
Cuts at: 0 546 819
Size: 546 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 434 755 819
Size: 312 122 321 64
Fragments arranged by size:
321 312 122 64
EcoO109I rG'GnC_Cy
Cuts at: 0 80 315 819
Size: 80 235 504
Fragments arranged by size:
504 235 80
EcoRV GAT'ATC
Cuts at: 0 456 819
Size: 456 363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 206 819
Size: 206 613
HaeII r_GCGC'y
Cuts at: 0 507 819
Size: 507 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 327 359 430 462 819
Size: 327 32 71 32 357
Fragments arranged by size:
357 327 71 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 819
Size: 218 601
MscI TGG'CCA
Cuts at: 0 694 819
Size: 694 125
MspA1I CmG'CkG
Cuts at: 0 45 598 819
Size: 45 553 221
Fragments arranged by size:
553 221 45
NcoI C'CATG_G
Cuts at: 0 319 819
Size: 319 500
NspI r_CATG'y
Cuts at: 0 370 819
Size: 370 449
PflMI CCAn_nnn'nTGG
Cuts at: 0 139 591 819
Size: 139 452 228
Fragments arranged by size:
452 228 139
PfoI T'CCnGG_A
Cuts at: 0 289 819
Size: 289 530
PpuMI rG'GwC_Cy
Cuts at: 0 80 315 819
Size: 80 235 504
Fragments arranged by size:
504 235 80
PvuII CAG'CTG
Cuts at: 0 598 819
Size: 598 221
SacI G_AGCT'C
Cuts at: 0 548 819
Size: 548 271
SacII CC_GC'GG
Cuts at: 0 46 819
Size: 46 773
SanDI GG'GwC_CC
Cuts at: 0 80 819
Size: 80 739
SmaI CCC'GGG
Cuts at: 0 78 819
Size: 78 741
StuI AGG'CCT
Cuts at: 0 433 819
Size: 433 386
StyI C'CwwG_G
Cuts at: 0 319 819
Size: 319 500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 285 317 819
Size: 285 32 502
Fragments arranged by size:
502 285 32
XmaI C'CCGG_G
Cuts at: 0 76 819
Size: 76 743
XmnI GAAnn'nnTTC
Cuts at: 0 189 819
Size: 189 630
Enzymes that do cut:
AcuI ApoI AvaI BanI BanII BglII BmrI BpmI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BsrDI BsrFI BssSI BstYI Bsu36I BtgI BtsI
CspCI EaeI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I MmeI MscI MspA1I NcoI NspI PflMI PfoI
PpuMI PvuII SacI SacII SanDI SmaI StuI StyI
TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsgI BsiEI BsiWI BsmI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
ZraI