Restriction map for
pF1KB0999
(Linear) (Six-base) MAPSORT of: KIBB0999.seq Check: 2486 from: 1 to: 7350
KIBB0999.orf
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 257 2767 4871 5249 5775 6075 6347 7350
Size: 257 2510 2104 378 526 300 272 1003
Fragments arranged by size:
2510 2104 1003 526 378 300 272 257
AatII G_ACGT'C
Cuts at: 0 110 3734 4783 6661 7350
Size: 110 3624 1049 1878 689
Fragments arranged by size:
3624 1878 1049 689 110
AccI GT'mk_AC
Cuts at: 0 4783 5807 7350
Size: 4783 1024 1543
Fragments arranged by size:
4783 1543 1024
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 598 734 2032 2183 2420 2908 2917
Size: 416 182 136 1298 151 237 488 9
Cuts at: 2917 3498 4258 4264 4567 5310 6822 7350
Size: 581 760 6 303 743 1512 528
Fragments arranged by size:
1512 1298 760 743 581 528 488 416
303 237 182 151 136 9 6
AfeI AGC'GCT
Cuts at: 0 901 7350
Size: 901 6449
AflIII A'CryG_T
Cuts at: 0 1289 7350
Size: 1289 6061
AgeI A'CCGG_T
Cuts at: 0 438 7350
Size: 438 6912
AleI CACnn'nnGTG
Cuts at: 0 30 3670 5482 5689 6440 7350
Size: 30 3640 1812 207 751 910
Fragments arranged by size:
3640 1812 910 751 207 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 694 3407 3688 7350
Size: 694 2713 281 3662
Fragments arranged by size:
3662 2713 694 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 874 906 6119 6151 7350
Size: 874 32 5213 32 1199
Fragments arranged by size:
5213 1199 874 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1390 2221 3147 4227 4963 6129 7350
Size: 1390 831 926 1080 736 1166 1221
Fragments arranged by size:
1390 1221 1166 1080 926 831 736
ApaI G_GGCC'C
Cuts at: 0 749 2300 3463 4234 7268 7350
Size: 749 1551 1163 771 3034 82
Fragments arranged by size:
3034 1551 1163 771 749 82
ApaLI G'TGCA_C
Cuts at: 0 2595 4483 7096 7350
Size: 2595 1888 2613 254
Fragments arranged by size:
2613 2595 1888 254
AvaI C'yCGr_G
Cuts at: 0 220 277 622 2724 2852 2954 3056 3376
Size: 220 57 345 2102 128 102 102 320
Cuts at: 3376 3777 4333 4511 4575 4825 5207 5852 5876
Size: 401 556 178 64 250 382 645 24
Cuts at: 5876 7053 7350
Size: 1177 297
Fragments arranged by size:
2102 1177 645 556 401 382 345 320
297 250 220 178 128 102 102 64
57 24
BamHI G'GATC_C
Cuts at: 0 7 4097 7350
Size: 7 4090 3253
Fragments arranged by size:
4090 3253 7
BanI G'GyrC_C
Cuts at: 0 486 1463 2190 2320 2728 3078 3391 3507
Size: 486 977 727 130 408 350 313 116
Cuts at: 3507 3579 3672 3745 4384 4597 4665 5269 5791
Size: 72 93 73 639 213 68 604 522
Cuts at: 5791 6060 6470 6595 7350
Size: 269 410 125 755
Fragments arranged by size:
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408 350 313 269 213 130 125 116
93 73 72 68
BanII G_rGCy'C
Cuts at: 0 50 116 245 749 1927 2300 2387 2415
Size: 50 66 129 504 1178 373 87 28
Cuts at: 2415 2582 2725 2951 3076 3463 3628 4234 4372
Size: 167 143 226 125 387 165 606 138
Cuts at: 4372 4585 4642 4718 5699 5828 5936 5959 5966
Size: 213 57 76 981 129 108 23 7
Cuts at: 5966 6188 6205 6233 6254 6785 6799 7268 7304
Size: 222 17 28 21 531 14 469 36
Cuts at: 7304 7350
Size: 46
Fragments arranged by size:
1178 981 606 531 504 469 387 373
226 222 213 167 165 143 138 129
129 125 108 87 76 66 57 50
46 36 28 28 23 21 17 14
7
BbeI G_GCGC'C
Cuts at: 0 1467 2324 2732 4601 6064 7350
Size: 1467 857 408 1869 1463 1286
Fragments arranged by size:
1869 1467 1463 1286 857 408
BbsI GAAGACnn'nnnn_
Cuts at: 0 404 1387 1634 1651 1734 7350
Size: 404 983 247 17 83 5616
Fragments arranged by size:
5616 983 404 247 83 17
BbvCI CC'TCA_GC
Cuts at: 0 2543 2648 3035 3240 3993 5152 7350
Size: 2543 105 387 205 753 1159 2198
Fragments arranged by size:
2543 2198 1159 753 387 205 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2062 2096 2420 2454 2734 2768 4849 4883
Size: 2062 34 324 34 280 34 2081 34
Cuts at: 4883 7350
Size: 2467
Fragments arranged by size:
2467 2081 2062 324 280 34 34 34
34
BclI T'GATC_A
Cuts at: 0 3209 7199 7350
Size: 3209 3990 151
Fragments arranged by size:
3990 3209 151
BglI GCCn_nnn'nGGC
Cuts at: 0 186 425 495 1070 1810 2650 2665 3059
Size: 186 239 70 575 740 840 15 394
Cuts at: 3059 3595 3977 5786 5871 7350
Size: 536 382 1809 85 1479
Fragments arranged by size:
1809 1479 840 740 575 536 394 382
239 186 85 70 15
BglII A'GATC_T
Cuts at: 0 566 1991 6959 7350
Size: 566 1425 4968 391
Fragments arranged by size:
4968 1425 566 391
BlpI GC'TnA_GC
Cuts at: 0 6487 7350
Size: 6487 863
Bme1580I G_kGCm'C
Cuts at: 0 749 2195 2300 2599 3197 3463 3584 3677
Size: 749 1446 105 299 598 266 121 93
Cuts at: 3677 3748 4171 4234 4387 4487 4518 4668 4826
Size: 71 423 63 153 100 31 150 158
Cuts at: 4826 5258 6600 6731 7100 7268 7350
Size: 432 1342 131 369 168 82
Fragments arranged by size:
1446 1342 749 598 432 423 369 299
266 168 158 153 150 131 121 105
100 93 82 71 63 31
BmgBI CAC'GTC
Cuts at: 0 32 3426 3825 3852 5396 5471 7350
Size: 32 3394 399 27 1544 75 1879
Fragments arranged by size:
3394 1879 1544 399 75 32 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 73 2500 3879 5642 5794 6203 6557 7350
Size: 73 2427 1379 1763 152 409 354 793
Fragments arranged by size:
2427 1763 1379 793 409 354 152 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 282 1830 5955 6876 7350
Size: 282 1548 4125 921 474
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 499 619 1174 1393 2733 5080 5523
Size: 139 360 120 555 219 1340 2347 443
Cuts at: 5523 5620 5950 6289 6367 6626 6791 7350
Size: 97 330 339 78 259 165 559
Fragments arranged by size:
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259 219 165 139 120 97 78
Bpu10I CC'TnA_GC
Cuts at: 0 473 2543 2648 3035 3240 3915 3993 4616
Size: 473 2070 105 387 205 675 78 623
Cuts at: 4616 5152 7350
Size: 536 2198
Fragments arranged by size:
2198 2070 675 623 536 473 387 205
105 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1621 2656 4345 4378 7350
Size: 1621 1035 1689 33 2972
Fragments arranged by size:
2972 1689 1621 1035 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 1306 1882 2482 4015 5411 5499 5637 6644
Size: 1306 576 600 1533 1396 88 138 1007
Cuts at: 6644 7017 7350
Size: 373 333
Fragments arranged by size:
1533 1396 1306 1007 600 576 373 333
138 88
BsaAI yAC'GTr
Cuts at: 0 4113 7350
Size: 4113 3237
BsaHI Gr'CG_yC
Cuts at: 0 107 1464 2119 2321 2729 3029 3598 3731
Size: 107 1357 655 202 408 300 569 133
Cuts at: 3731 4508 4598 4780 4786 6061 6658 7126 7350
Size: 777 90 182 6 1275 597 468 224
Fragments arranged by size:
1357 1275 777 655 597 569 468 408
300 224 202 182 133 107 90 6
BsaWI w'CCGG_w
Cuts at: 0 438 701 2358 2407 3564 6705 6917 6982
Size: 438 263 1657 49 1157 3141 212 65
Cuts at: 6982 7350
Size: 368
Fragments arranged by size:
3141 1657 1157 438 368 263 212 65
49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 763 793 874 904 2731 2761
Size: 133 30 600 30 81 30 1827 30
Cuts at: 2761 3523 3553 3688 3718 4429 4459 4912 4942
Size: 762 30 135 30 711 30 453 30
Cuts at: 4942 5329 5359 5496 5526 6119 6149 7232 7262
Size: 387 30 137 30 593 30 1083 30
Cuts at: 7262 7350
Size: 88
Fragments arranged by size:
1827 1083 762 711 600 593 453 387
137 135 133 88 81 30 30 30
30 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 616 619 870 1567 1609 1612 1624
Size: 523 93 3 251 697 42 3 12
Cuts at: 1624 1792 2089 2122 2155 2158 2170 2249 2444
Size: 168 297 33 33 3 12 79 195
Cuts at: 2444 2488 2497 2500 2527 2536 2560 2590 2626
Size: 44 9 3 27 9 24 30 36
Cuts at: 2626 2821 3342 3981 4330 4552 5724 5931 7080
Size: 195 521 639 349 222 1172 207 1149
Cuts at: 7080 7312 7350
Size: 232 38
Fragments arranged by size:
1172 1149 697 639 523 521 349 297
251 232 222 207 195 195 168 93
79 44 42 38 36 33 33 30
27 24 12 12 9 9 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 182 282 451 1793 2214 2307 2824 2988
Size: 182 100 169 1342 421 93 517 164
Cuts at: 2988 3120 3367 3556 3661 4620 5135 5274 5318
Size: 132 247 189 105 959 515 139 44
Cuts at: 5318 5698 5913 5947 6431 6784 7024 7350
Size: 380 215 34 484 353 240 326
Fragments arranged by size:
1342 959 517 515 484 421 380 353
326 247 240 215 189 182 169 164
139 132 105 100 93 44 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 700 704 2432 2786 7350
Size: 103 597 4 1728 354 4564
Fragments arranged by size:
4564 1728 597 354 103 4
BsiEI CG_ry'CG
Cuts at: 0 358 1094 1893 2660 3798 3938 4357 4467
Size: 358 736 799 767 1138 140 419 110
Cuts at: 4467 6346 7350
Size: 1879 1004
Fragments arranged by size:
1879 1138 1004 799 767 736 419 358
140 110
BsiHKAI G_wGCw'C
Cuts at: 0 245 1109 2599 2725 3892 4487 4832 4862
Size: 245 864 1490 126 1167 595 345 30
Cuts at: 4862 5252 5492 6205 6254 7100 7350
Size: 390 240 713 49 846 250
Fragments arranged by size:
1490 1167 864 846 713 595 390 345
250 245 240 126 49 30
BsiWI C'GTAC_G
Cuts at: 0 4440 7350
Size: 4440 2910
BsmI GAATG_Cn'
Cuts at: 0 2083 2209 3656 7350
Size: 2083 126 1447 3694
Fragments arranged by size:
3694 2083 1447 126
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1443 3925 7350
Size: 1443 2482 3425
Fragments arranged by size:
3425 2482 1443
Bsp1286I G_dGCh'C
Cuts at: 0 50 116 245 749 1109 1927 2195 2300
Size: 50 66 129 504 360 818 268 105
Cuts at: 2300 2387 2415 2582 2599 2725 2951 3076 3197
Size: 87 28 167 17 126 226 125 121
Cuts at: 3197 3463 3584 3628 3677 3748 3892 4171 4234
Size: 266 121 44 49 71 144 279 63
Cuts at: 4234 4372 4387 4487 4518 4585 4642 4668 4718
Size: 138 15 100 31 67 57 26 50
Cuts at: 4718 4826 4832 4862 5252 5258 5492 5699 5828
Size: 108 6 30 390 6 234 207 129
Cuts at: 5828 5936 5959 5966 6188 6205 6233 6254 6600
Size: 108 23 7 222 17 28 21 346
Cuts at: 6600 6731 6785 6799 7100 7268 7304 7350
Size: 131 54 14 301 168 36 46
Fragments arranged by size:
818 504 390 360 346 301 279 268
266 234 226 222 207 168 167 144
138 131 129 129 126 125 121 121
108 108 105 100 87 71 67 66
63 57 54 50 50 49 46 44
36 31 30 28 28 26 23 21
17 17 15 14 7 6 6
BspHI T'CATG_A
Cuts at: 0 798 1283 7350
Size: 798 485 6067
Fragments arranged by size:
6067 798 485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 257 281 2767 3020 4142 4871 4982 5249
Size: 257 24 2486 253 1122 729 111 267
Cuts at: 5249 5775 6075 6347 7159 7350
Size: 526 300 272 812 191
Fragments arranged by size:
2486 1122 812 729 526 300 272 267
257 253 191 111 24
BsrBI CCG'CTC
Cuts at: 0 1751 2132 2699 2908 4292 4359 4392 5409
Size: 1751 381 567 209 1384 67 33 1017
Cuts at: 5409 5433 5451 5871 5921 7131 7350
Size: 24 18 420 50 1210 219
Fragments arranged by size:
1751 1384 1210 1017 567 420 381 219
209 67 50 33 24 18
BsrDI GCAATG_nn'
Cuts at: 0 4586 7350
Size: 4586 2764
BsrFI r'CCGG_y
Cuts at: 0 438 2058 2619 2804 4248 4405 4553 4675
Size: 438 1620 561 185 1444 157 148 122
Cuts at: 4675 5066 5887 6057 7073 7350
Size: 391 821 170 1016 277
Fragments arranged by size:
1620 1444 1016 821 561 438 391 277
185 170 157 148 122
BsrGI T'GTAC_A
Cuts at: 0 767 1265 3737 7350
Size: 767 498 2472 3613
Fragments arranged by size:
3613 2472 767 498
BssHII G'CGCG_C
Cuts at: 0 4591 7350
Size: 4591 2759
BssSI C'ACGA_G
Cuts at: 0 1108 4688 4790 5251 7350
Size: 1108 3580 102 461 2099
Fragments arranged by size:
3580 2099 1108 461 102
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1533 3012 4672 4963 5106 7350
Size: 1533 1479 1660 291 143 2244
Fragments arranged by size:
2244 1660 1533 1479 291 143
BstEII G'GTnAC_C
Cuts at: 0 2931 3265 6148 6406 6420 7350
Size: 2931 334 2883 258 14 930
Fragments arranged by size:
2931 2883 930 334 258 14
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3504 5130 5286 5484 6243 6792 7350
Size: 3504 1626 156 198 759 549 558
Fragments arranged by size:
3504 1626 759 558 549 198 156
BstYI r'GATC_y
Cuts at: 0 7 391 566 1016 1991 4097 4304 6959
Size: 7 384 175 450 975 2106 207 2655
Cuts at: 6959 7350
Size: 391
Fragments arranged by size:
2655 2106 975 450 391 384 207 175
7
Bsu36I CC'TnA_GG
Cuts at: 0 1659 5716 7350
Size: 1659 4057 1634
Fragments arranged by size:
4057 1659 1634
BtgI C'CryG_G
Cuts at: 0 374 493 551 1467 1817 2093 2537 2654
Size: 374 119 58 916 350 276 444 117
Cuts at: 2654 3470 3488 3544 3699 3714 3792 4077 4271
Size: 816 18 56 155 15 78 285 194
Cuts at: 4271 4351 4630 4659 4938 5034 5129 5285 5708
Size: 80 279 29 279 96 95 156 423
Cuts at: 5708 6153 6591 6606 6866 7253 7350
Size: 445 438 15 260 387 97
Fragments arranged by size:
916 816 445 444 438 423 387 374
350 285 279 279 276 260 194 156
155 119 117 97 96 95 80 78
58 56 29 18 15 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 838 1893 2423 5988 7350
Size: 838 1055 530 3565 1362
Fragments arranged by size:
3565 1362 1055 838 530
BtsI GCAGTG_nn'
Cuts at: 0 1080 1948 2214 2561 2769 3271 3293 5775
Size: 1080 868 266 347 208 502 22 2482
Cuts at: 5775 7309 7350
Size: 1534 41
Fragments arranged by size:
2482 1534 1080 868 502 347 266 208
41 22
ClaI AT'CG_AT
Cuts at: 0 1127 7350
Size: 1127 6223
DraIII CAC_nnn'GTG
Cuts at: 0 1113 6565 7350
Size: 1113 5452 785
Fragments arranged by size:
5452 1113 785
DrdI GACnn_nn'nnGTC
Cuts at: 0 1279 7350
Size: 1279 6071
EaeI y'GGCC_r
Cuts at: 0 253 371 1091 1175 1247 1890 1904 2066
Size: 253 118 720 84 72 643 14 162
Cuts at: 2066 2621 2657 3795 3935 4268 4354 4464 4502
Size: 555 36 1138 140 333 86 110 38
Cuts at: 4502 4555 4677 5120 5863 5889 5991 6343 7075
Size: 53 122 443 743 26 102 352 732
Cuts at: 7075 7350
Size: 275
Fragments arranged by size:
1138 743 732 720 643 555 443 352
333 275 253 162 140 122 118 110
102 86 84 72 53 38 36 26
14
EagI C'GGCC_G
Cuts at: 0 1091 1890 2657 3795 3935 4354 4464 6343
Size: 1091 799 767 1138 140 419 110 1879
Cuts at: 6343 7350
Size: 1007
Fragments arranged by size:
1879 1138 1091 1007 799 767 419 140
110
EarI CTCTTCn'nnn_
Cuts at: 0 1966 4543 6242 7350
Size: 1966 2577 1699 1108
Fragments arranged by size:
2577 1966 1699 1108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 204 1600 3653 4166 6541 6852 7350
Size: 204 1396 2053 513 2375 311 498
Fragments arranged by size:
2375 2053 1396 513 498 311 204
EcoICRI GAG'CTC
Cuts at: 0 243 2723 6203 6252 7350
Size: 243 2480 3480 49 1098
Fragments arranged by size:
3480 2480 1098 243 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 416 499 598 619 734 1174 1393
Size: 139 277 83 99 21 115 440 219
Cuts at: 1393 2032 2183 2420 2733 2908 2917 3498 4258
Size: 639 151 237 313 175 9 581 760
Cuts at: 4258 4264 4567 5080 5310 5523 5620 5950 6289
Size: 6 303 513 230 213 97 330 339
Cuts at: 6289 6367 6626 6791 6822 7350
Size: 78 259 165 31 528
Fragments arranged by size:
760 639 581 528 513 440 339 330
313 303 277 259 237 230 219 213
175 165 151 139 115 99 97 83
78 31 21 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 1636 3739 3814 4204 4732 7350
Size: 406 1230 2103 75 390 528 2618
Fragments arranged by size:
2618 2103 1230 528 406 390 75
EcoO109I rG'GnC_Cy
Cuts at: 0 194 745 746 3156 3450 3460 3919 4197
Size: 194 551 1 2410 294 10 459 278
Cuts at: 4197 4224 4231 4260 5157 5203 5847 6298 7157
Size: 27 7 29 897 46 644 451 859
Cuts at: 7157 7265 7350
Size: 108 85
Fragments arranged by size:
2410 897 859 644 551 459 451 294
278 194 108 85 46 29 27 10
7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 858 1332 7350
Size: 858 474 6018
Fragments arranged by size:
6018 858 474
FseI GG_CCGG'CC
Cuts at: 0 2623 7350
Size: 2623 4727
HaeII r_GCGC'y
Cuts at: 0 321 903 1467 1805 2059 2324 2503 2671
Size: 321 582 564 338 254 265 179 168
Cuts at: 2671 2732 3689 4601 5059 6064 7350
Size: 61 957 912 458 1005 1286
Fragments arranged by size:
1286 1005 957 912 582 564 458 338
321 265 254 179 168 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 846 878 1120 1152 1642 1674
Size: 133 32 681 32 242 32 490 32
Cuts at: 1674 2731 2763 3523 3555 4429 4461 4912 4944
Size: 1057 32 760 32 874 32 451 32
Cuts at: 4944 5201 5233 7144 7176 7350
Size: 257 32 1911 32 174
Fragments arranged by size:
1911 1057 874 760 681 490 451 257
242 174 133 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2226 4784 7350
Size: 2226 2558 2566
Fragments arranged by size:
2566 2558 2226
HindIII A'AGCT_T
Cuts at: 0 6307 7350
Size: 6307 1043
KasI G'GCGC_C
Cuts at: 0 1463 2320 2728 4597 6060 7350
Size: 1463 857 408 1869 1463 1290
Fragments arranged by size:
1869 1463 1463 1290 857 408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 3356 5392 7350
Size: 806 2550 2036 1958
Fragments arranged by size:
2550 2036 1958 806
MscI TGG'CCA
Cuts at: 0 373 4270 5993 7350
Size: 373 3897 1723 1357
Fragments arranged by size:
3897 1723 1357 373
MslI CAynn'nnrTG
Cuts at: 0 30 1231 1261 1288 3670 3877 5128 5284
Size: 30 1201 30 27 2382 207 1251 156
Cuts at: 5284 5482 5588 5668 5689 6440 7350
Size: 198 106 80 21 751 910
Fragments arranged by size:
2382 1251 1201 910 751 207 198 156
106 80 30 30 27 21
MspA1I CmG'CkG
Cuts at: 0 186 597 765 1068 1173 1469 2539 2647
Size: 186 411 168 303 105 296 1070 108
Cuts at: 2647 2895 3518 3537 3701 3727 3794 4079 4353
Size: 248 623 19 164 26 67 285 274
Cuts at: 4353 4491 4632 4940 4963 5036 5528 5537 5546
Size: 138 141 308 23 73 492 9 9
Cuts at: 5546 5689 6348 6378 6414 6533 6753 7255 7262
Size: 143 659 30 36 119 220 502 7
Cuts at: 7262 7350
Size: 88
Fragments arranged by size:
1070 659 623 502 492 411 308 303
296 285 274 248 220 186 168 164
143 141 138 119 108 105 88 73
67 36 30 26 23 19 9 9
7
NaeI GCC'GGC
Cuts at: 0 2060 2621 4250 4555 4677 6059 7350
Size: 2060 561 1629 305 122 1382 1291
Fragments arranged by size:
2060 1629 1382 1291 561 305 122
NarI GG'CG_CC
Cuts at: 0 1464 2321 2729 4598 6061 7350
Size: 1464 857 408 1869 1463 1289
Fragments arranged by size:
1869 1464 1463 1289 857 408
NcoI C'CATG_G
Cuts at: 0 551 1817 3470 3488 5129 5285 6591 6866
Size: 551 1266 1653 18 1641 156 1306 275
Cuts at: 6866 7350
Size: 484
Fragments arranged by size:
1653 1641 1306 1266 551 484 275 156
18
NgoMIV G'CCGG_C
Cuts at: 0 2058 2619 4248 4553 4675 6057 7350
Size: 2058 561 1629 305 122 1382 1293
Fragments arranged by size:
2058 1629 1382 1293 561 305 122
NotI GC'GGCC_GC
Cuts at: 0 4354 6343 7350
Size: 4354 1989 1007
Fragments arranged by size:
4354 1989 1007
NspI r_CATG'y
Cuts at: 0 1050 1293 2176 3229 3445 3876 7350
Size: 1050 243 883 1053 216 431 3474
Fragments arranged by size:
3474 1053 1050 883 431 243 216
PasI CC'CwG_GG
Cuts at: 0 2316 3279 4227 6394 6523 7350
Size: 2316 963 948 2167 129 827
Fragments arranged by size:
2316 2167 963 948 827 129
PciI A'CATG_T
Cuts at: 0 1289 7350
Size: 1289 6061
PflMI CCAn_nnn'nTGG
Cuts at: 0 525 915 6129 6417 7350
Size: 525 390 5214 288 933
Fragments arranged by size:
5214 933 525 390 288
PfoI T'CCnGG_A
Cuts at: 0 116 1319 1370 1880 6122 6542 6733 7350
Size: 116 1203 51 510 4242 420 191 617
Fragments arranged by size:
4242 1203 617 510 420 191 116 51
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 874 906 991 1023 6119 6151
Size: 286 32 556 32 85 32 5096 32
Cuts at: 6151 7350
Size: 1199
Fragments arranged by size:
5096 1199 556 286 85 32 32 32
32
PpuMI rG'GwC_Cy
Cuts at: 0 3156 3919 4197 5203 7350
Size: 3156 763 278 1006 2147
Fragments arranged by size:
3156 2147 1006 763 278
PshAI GACnn'nnGTC
Cuts at: 0 6661 7350
Size: 6661 689
PspOMI G'GGCC_C
Cuts at: 0 745 2296 3459 4230 7264 7350
Size: 745 1551 1163 771 3034 86
Fragments arranged by size:
3034 1551 1163 771 745 86
PstI C_TGCA'G
Cuts at: 0 1040 2764 3017 4733 7214 7350
Size: 1040 1724 253 1716 2481 136
Fragments arranged by size:
2481 1724 1716 1040 253 136
PvuII CAG'CTG
Cuts at: 0 597 1173 2895 3537 3727 6533 7350
Size: 597 576 1722 642 190 2806 817
Fragments arranged by size:
2806 1722 817 642 597 576 190
SacI G_AGCT'C
Cuts at: 0 245 2725 6205 6254 7350
Size: 245 2480 3480 49 1096
Fragments arranged by size:
3480 2480 1096 245 49
SacII CC_GC'GG
Cuts at: 0 1470 2540 3702 3795 4080 4354 4633 4941
Size: 1470 1070 1162 93 285 274 279 308
Cuts at: 4941 5037 7256 7350
Size: 96 2219 94
Fragments arranged by size:
2219 1470 1162 1070 308 285 279 274
96 94 93
SalI G'TCGA_C
Cuts at: 0 4782 7350
Size: 4782 2568
SanDI GG'GwC_CC
Cuts at: 0 3156 5203 7350
Size: 3156 2047 2147
Fragments arranged by size:
3156 2147 2047
SapI GCTCTTCn'nnn_
Cuts at: 0 4543 6242 7350
Size: 4543 1699 1108
Fragments arranged by size:
4543 1699 1108
SbfI CC_TGCA'GG
Cuts at: 0 4733 7350
Size: 4733 2617
ScaI AGT'ACT
Cuts at: 0 994 7350
Size: 994 6356
SexAI A'CCwGG_T
Cuts at: 0 3261 5508 7350
Size: 3261 2247 1842
Fragments arranged by size:
3261 2247 1842
SfcI C'TryA_G
Cuts at: 0 1036 2760 3013 4729 6165 7210 7350
Size: 1036 1724 253 1716 1436 1045 140
Fragments arranged by size:
1724 1716 1436 1045 1036 253 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2650 5871 7350
Size: 2650 3221 1479
Fragments arranged by size:
3221 2650 1479
SfoI GGC'GCC
Cuts at: 0 1465 2322 2730 4599 6062 7350
Size: 1465 857 408 1869 1463 1288
Fragments arranged by size:
1869 1465 1463 1288 857 408
SgrAI Cr'CCGG_yG
Cuts at: 0 2058 4405 6057 7350
Size: 2058 2347 1652 1293
Fragments arranged by size:
2347 2058 1652 1293
SmaI CCC'GGG
Cuts at: 0 222 2854 2956 3058 4513 5854 7055 7350
Size: 222 2632 102 102 1455 1341 1201 295
Fragments arranged by size:
2632 1455 1341 1201 295 222 102 102
SmlI C'TyrA_G
Cuts at: 0 1636 2671 3376 4360 4393 7350
Size: 1636 1035 705 984 33 2957
Fragments arranged by size:
2957 1636 1035 984 705 33
SphI G_CATG'C
Cuts at: 0 1050 3229 3445 7350
Size: 1050 2179 216 3905
Fragments arranged by size:
3905 2179 1050 216
SrfI GCCC'GGGC
Cuts at: 0 4513 7350
Size: 4513 2837
StuI AGG'CCT
Cuts at: 0 740 922 3217 4210 7350
Size: 740 182 2295 993 3140
Fragments arranged by size:
3140 2295 993 740 182
StyI C'CwwG_G
Cuts at: 0 407 503 551 971 1817 2276 2468 2624
Size: 407 96 48 420 846 459 192 156
Cuts at: 2624 2818 3402 3446 3470 3488 3815 3893 5129
Size: 194 584 44 24 18 327 78 1236
Cuts at: 5129 5285 5840 6591 6866 7007 7350
Size: 156 555 751 275 141 343
Fragments arranged by size:
1236 846 751 584 555 459 420 407
343 327 275 194 192 156 156 141
96 78 48 44 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 554 2361 3284 3336 3402 3727 4094 4219
Size: 554 1807 923 52 66 325 367 125
Cuts at: 4219 4784 4850 5511 5788 6222 6491 6576 6651
Size: 565 66 661 277 434 269 85 75
Cuts at: 6651 6658 6865 6925 7350
Size: 7 207 60 425
Fragments arranged by size:
1807 923 661 565 554 434 425 367
325 277 269 207 125 85 75 66
66 60 52 7
TatI w'GTAC_w
Cuts at: 0 767 845 992 1265 3737 7350
Size: 767 78 147 273 2472 3613
Fragments arranged by size:
3613 2472 767 273 147 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 3841 3873 4082 4114 5329 5361
Size: 55 32 3754 32 209 32 1215 32
Cuts at: 5361 7232 7264 7350
Size: 1871 32 86
Fragments arranged by size:
3754 1871 1215 209 86 55 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1935 7350
Size: 1935 5415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 1072 1914 3148 3453 5481 6126 7350
Size: 18 1054 842 1234 305 2028 645 1224
Fragments arranged by size:
2028 1234 1224 1054 842 645 305 18
XhoI C'TCGA_G
Cuts at: 0 3376 7350
Size: 3376 3974
XmaI C'CCGG_G
Cuts at: 0 220 2852 2954 3056 4511 5852 7053 7350
Size: 220 2632 102 102 1455 1341 1201 297
Fragments arranged by size:
2632 1455 1341 1201 297 220 102 102
XmnI GAAnn'nnTTC
Cuts at: 0 1006 7350
Size: 1006 6344
ZraI GAC'GTC
Cuts at: 0 108 3732 4781 6659 7350
Size: 108 3624 1049 1878 691
Fragments arranged by size:
3624 1878 1049 691 108
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AhdI ApoI AscI AseI AsiSI
AvrII BaeI BciVI BfrBI BmtI BsaBI BspEI BstBI
BstZ17I CspCI DraI EcoRI EcoRV FspI FspAI HpaI
KpnI MfeI MluI NdeI NheI NruI NsiI PacI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SgfI
SnaBI SpeI SspI SwaI XbaI