Restriction map for
pF1KB1123
(Linear) (Six-base) MAPSORT of: KIBB1123.seq Check: 9243 from: 1 to: 7647
KIBB1123.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1419 1882 2502 7647
Size: 1419 463 620 5145
Fragments arranged by size:
5145 1419 620 463
AatII G_ACGT'C
Cuts at: 0 5865 7647
Size: 5865 1782
AccI GT'mk_AC
Cuts at: 0 178 5944 7647
Size: 178 5766 1703
Fragments arranged by size:
5766 1703 178
Acc65I G'GTAC_C
Cuts at: 0 5897 7647
Size: 5897 1750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2347 2635 3395 3895 4073 6004 6214 7647
Size: 2347 288 760 500 178 1931 210 1433
Fragments arranged by size:
2347 1931 1433 760 500 288 210 178
AfeI AGC'GCT
Cuts at: 0 2044 3404 3622 7647
Size: 2044 1360 218 4025
Fragments arranged by size:
4025 2044 1360 218
AflIII A'CryG_T
Cuts at: 0 5108 5990 7647
Size: 5108 882 1657
Fragments arranged by size:
5108 1657 882
AhdI GACnn_n'nnGTC
Cuts at: 0 3528 7647
Size: 3528 4119
AleI CACnn'nnGTG
Cuts at: 0 1066 1555 4683 7647
Size: 1066 489 3128 2964
Fragments arranged by size:
3128 2964 1066 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 89 998 1477 4159 4701 4992 6820 7647
Size: 89 909 479 2682 542 291 1828 827
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3019 3051 4193 4225 7647
Size: 3019 32 1142 32 3422
Fragments arranged by size:
3422 3019 1142 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 879 1999 2362 2967 3045 5226 6192 7647
Size: 879 1120 363 605 78 2181 966 1455
Fragments arranged by size:
2181 1455 1120 966 879 605 363 78
ApaI G_GGCC'C
Cuts at: 0 3915 4111 6184 7647
Size: 3915 196 2073 1463
Fragments arranged by size:
3915 2073 1463 196
ApoI r'AATT_y
Cuts at: 0 436 1105 1777 2607 3028 3118 3196 3566
Size: 436 669 672 830 421 90 78 370
Cuts at: 3566 4084 7367 7647
Size: 518 3283 280
Fragments arranged by size:
3283 830 672 669 518 436 421 370
280 90 78
AseI AT'TA_AT
Cuts at: 0 2335 4256 7647
Size: 2335 1921 3391
Fragments arranged by size:
3391 2335 1921
AvaI C'yCGr_G
Cuts at: 0 1469 2726 4450 4610 4825 6699 7100 7647
Size: 1469 1257 1724 160 215 1874 401 547
Fragments arranged by size:
1874 1724 1469 1257 547 401 215 160
BamHI G'GATC_C
Cuts at: 0 779 2682 3681 7647
Size: 779 1903 999 3966
Fragments arranged by size:
3966 1903 999 779
BanI G'GyrC_C
Cuts at: 0 2273 3310 5643 5897 7634 7647
Size: 2273 1037 2333 254 1737 13
Fragments arranged by size:
2333 2273 1737 1037 254 13
BanII G_rGCy'C
Cuts at: 0 131 456 1520 2570 3499 3915 4010 4111
Size: 131 325 1064 1050 929 416 95 101
Cuts at: 4111 6158 6184 7647
Size: 2047 26 1463
Fragments arranged by size:
2047 1463 1064 1050 929 416 325 131
101 95 26
BbeI G_GCGC'C
Cuts at: 0 3314 7647
Size: 3314 4333
BbsI GAAGACnn'nnnn_
Cuts at: 0 1437 2623 3234 3524 4949 7647
Size: 1437 1186 611 290 1425 2698
Fragments arranged by size:
2698 1437 1425 1186 611 290
BbvCI CC'TCA_GC
Cuts at: 0 5819 6347 6658 7647
Size: 5819 528 311 989
Fragments arranged by size:
5819 989 528 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 663 697 1959 1993 7647
Size: 663 34 1262 34 5654
Fragments arranged by size:
5654 1262 663 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3649 5697 7647
Size: 3649 2048 1950
Fragments arranged by size:
3649 2048 1950
BclI T'GATC_A
Cuts at: 0 1110 2381 7647
Size: 1110 1271 5266
Fragments arranged by size:
5266 1271 1110
BfrBI ATG'CAT
Cuts at: 0 3249 3730 7647
Size: 3249 481 3917
Fragments arranged by size:
3917 3249 481
BglI GCCn_nnn'nGGC
Cuts at: 0 1664 4381 4534 4543 4979 5464 5500 6710
Size: 1664 2717 153 9 436 485 36 1210
Cuts at: 6710 7178 7647
Size: 468 469
Fragments arranged by size:
2717 1664 1210 485 469 468 436 153
36 9
BglII A'GATC_T
Cuts at: 0 903 4850 6570 7647
Size: 903 3947 1720 1077
Fragments arranged by size:
3947 1720 1077 903
BlpI GC'TnA_GC
Cuts at: 0 1966 3859 7647
Size: 1966 1893 3788
Fragments arranged by size:
3788 1966 1893
Bme1580I G_kGCm'C
Cuts at: 0 2262 2276 3915 4111 5124 6184 7639 7647
Size: 2262 14 1639 196 1013 1060 1455 8
Fragments arranged by size:
2262 1639 1455 1060 1013 196 14 8
BmgBI CAC'GTC
Cuts at: 0 3777 7647
Size: 3777 3870
BmrI ACTGGGnnnn_n'
Cuts at: 0 352 1040 1224 7647
Size: 352 688 184 6423
Fragments arranged by size:
6423 688 352 184
BmtI G_CTAG'C
Cuts at: 0 275 7647
Size: 275 7372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7537 7647
Size: 7537 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1534 2467 3886 4225 4348 4816 5683
Size: 115 1419 933 1419 339 123 468 867
Cuts at: 5683 6172 7305 7647
Size: 489 1133 342
Fragments arranged by size:
1419 1419 1133 933 867 489 468 342
339 123 115
Bpu10I CC'TnA_GC
Cuts at: 0 1261 1570 3709 5819 6347 6658 7647
Size: 1261 309 2139 2110 528 311 989
Fragments arranged by size:
2139 2110 1261 989 528 311 309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2614 4945 7647
Size: 2614 2331 2702
Fragments arranged by size:
2702 2614 2331
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 2286 2873 3828 4513 7647
Size: 733 1553 587 955 685 3134
Fragments arranged by size:
3134 1553 955 733 685 587
BsaAI yAC'GTr
Cuts at: 0 432 7647
Size: 432 7215
BsaBI GATnn'nnATC
Cuts at: 0 2200 2869 7647
Size: 2200 669 4778
Fragments arranged by size:
4778 2200 669
BsaHI Gr'CG_yC
Cuts at: 0 1436 2029 3311 5862 7647
Size: 1436 593 1282 2551 1785
Fragments arranged by size:
2551 1785 1436 1282 593
BsaWI w'CCGG_w
Cuts at: 0 10 6816 7647
Size: 10 6806 831
Fragments arranged by size:
6806 831 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1063 1093 2266 2296 3439 3469 4654 4684
Size: 1063 30 1173 30 1143 30 1185 30
Cuts at: 4684 7647
Size: 2963
Fragments arranged by size:
2963 1185 1173 1143 1063 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 331 1114 3691 4813 6055 7647
Size: 187 144 783 2577 1122 1242 1592
Fragments arranged by size:
2577 1592 1242 1122 783 187 144
BseYI C'CCAG_C
Cuts at: 0 465 662 971 999 2268 2841 3045 3215
Size: 465 197 309 28 1269 573 204 170
Cuts at: 3215 3549 3908 4146 4431 5183 6713 6883 6922
Size: 334 359 238 285 752 1530 170 39
Cuts at: 6922 7647
Size: 725
Fragments arranged by size:
1530 1269 752 725 573 465 359 334
309 285 238 204 197 170 170 39
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2121 2980 3757 4342 5212 6273 6721 7045
Size: 2121 859 777 585 870 1061 448 324
Cuts at: 7045 7647
Size: 602
Fragments arranged by size:
2121 1061 870 859 777 602 585 448
324
BsiEI CG_ry'CG
Cuts at: 0 4289 5349 5504 7647
Size: 4289 1060 155 2143
Fragments arranged by size:
4289 2143 1060 155
BsiHKAI G_wGCw'C
Cuts at: 0 131 1562 1844 2228 2570 3151 3635 4010
Size: 131 1431 282 384 342 581 484 375
Cuts at: 4010 5302 5345 5566 6002 7229 7647
Size: 1292 43 221 436 1227 418
Fragments arranged by size:
1431 1292 1227 581 484 436 418 384
375 342 282 221 131 43
BsmI GAATG_Cn'
Cuts at: 0 303 1201 6455 7647
Size: 303 898 5254 1192
Fragments arranged by size:
5254 1192 898 303
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3846 5048 5869 7445 7647
Size: 3846 1202 821 1576 202
Fragments arranged by size:
3846 1576 1202 821 202
Bsp1286I G_dGCh'C
Cuts at: 0 131 456 1520 1562 1844 2228 2262 2276
Size: 131 325 1064 42 282 384 34 14
Cuts at: 2276 2570 3151 3499 3635 3915 4010 4111 5124
Size: 294 581 348 136 280 95 101 1013
Cuts at: 5124 5302 5345 5566 6002 6158 6184 7229 7639
Size: 178 43 221 436 156 26 1045 410
Cuts at: 7639 7647
Size: 8
Fragments arranged by size:
1064 1045 1013 581 436 410 384 348
325 294 282 280 221 178 156 136
131 101 95 43 42 34 26 14
8
BspEI T'CCGG_A
Cuts at: 0 6816 7647
Size: 6816 831
BspHI T'CATG_A
Cuts at: 0 3110 3128 4938 7542 7647
Size: 3110 18 1810 2604 105
Fragments arranged by size:
3110 2604 1810 105 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 1419 1882 2502 3218 3338 4715 7647
Size: 24 1395 463 620 716 120 1377 2932
Fragments arranged by size:
2932 1395 1377 716 620 463 120 24
BsrBI CCG'CTC
Cuts at: 0 1381 7647
Size: 1381 6266
BsrDI GCAATG_nn'
Cuts at: 0 57 486 1430 1481 4736 5825 7647
Size: 57 429 944 51 3255 1089 1822
Fragments arranged by size:
3255 1822 1089 944 429 57 51
BsrFI r'CCGG_y
Cuts at: 0 1076 7647
Size: 1076 6571
BsrGI T'GTAC_A
Cuts at: 0 963 5636 7647
Size: 963 4673 2011
Fragments arranged by size:
4673 2011 963
BssSI C'ACGA_G
Cuts at: 0 4360 5684 7647
Size: 4360 1324 1963
Fragments arranged by size:
4360 1963 1324
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2967 3339 3705 4445 5226 7647
Size: 2967 372 366 740 781 2421
Fragments arranged by size:
2967 2421 781 740 372 366
BstBI TT'CG_AA
Cuts at: 0 3480 5393 7647
Size: 3480 1913 2254
Fragments arranged by size:
3480 2254 1913
BstEII G'GTnAC_C
Cuts at: 0 5085 7647
Size: 5085 2562
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1068 1680 1734 3307 3519 5853 7158 7647
Size: 1068 612 54 1573 212 2334 1305 489
Fragments arranged by size:
2334 1573 1305 1068 612 489 212 54
BstYI r'GATC_y
Cuts at: 0 779 903 1850 1995 2117 2352 2682 2870
Size: 779 124 947 145 122 235 330 188
Cuts at: 2870 3681 3945 4331 4850 5613 6570 6773 7055
Size: 811 264 386 519 763 957 203 282
Cuts at: 7055 7647
Size: 592
Fragments arranged by size:
957 947 811 779 763 592 519 386
330 282 264 235 203 188 145 124
122
Bsu36I CC'TnA_GG
Cuts at: 0 1721 5081 7647
Size: 1721 3360 2566
Fragments arranged by size:
3360 2566 1721
BtgI C'CryG_G
Cuts at: 0 146 401 783 2852 2906 3518 4766 5498
Size: 146 255 382 2069 54 612 1248 732
Cuts at: 5498 7647
Size: 2149
Fragments arranged by size:
2149 2069 1248 732 612 382 255 146
54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1745 3872 4032 5178 5447 5570 6778 7647
Size: 1745 2127 160 1146 269 123 1208 869
Fragments arranged by size:
2127 1745 1208 1146 869 269 160 123
BtsI GCAGTG_nn'
Cuts at: 0 1535 1550 1562 1949 3382 4492 4651 5387
Size: 1535 15 12 387 1433 1110 159 736
Cuts at: 5387 5486 5507 5519 6733 7647
Size: 99 21 12 1214 914
Fragments arranged by size:
1535 1433 1214 1110 914 736 387 159
99 21 15 12 12
ClaI AT'CG_AT
Cuts at: 0 5132 7647
Size: 5132 2515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 291 326 1412 1447 7647
Size: 291 35 1086 35 6200
Fragments arranged by size:
6200 1086 291 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2700 7206 7647
Size: 2700 4506 441
Fragments arranged by size:
4506 2700 441
DrdI GACnn_nn'nnGTC
Cuts at: 0 361 1441 7647
Size: 361 1080 6206
Fragments arranged by size:
6206 1080 361
EaeI y'GGCC_r
Cuts at: 0 263 390 524 587 746 1914 2129 2501
Size: 263 127 134 63 159 1168 215 372
Cuts at: 2501 2543 3717 3938 4286 5501 5746 6056 6626
Size: 42 1174 221 348 1215 245 310 570
Cuts at: 6626 6896 7244 7353 7647
Size: 270 348 109 294
Fragments arranged by size:
1215 1174 1168 570 372 348 348 310
294 270 263 245 221 215 159 134
127 109 63 42
EagI C'GGCC_G
Cuts at: 0 4286 5501 7647
Size: 4286 1215 2146
Fragments arranged by size:
4286 2146 1215
EarI CTCTTCn'nnn_
Cuts at: 0 3064 3668 3955 4672 5110 6089 6607 6650
Size: 3064 604 287 717 438 979 518 43
Cuts at: 6650 7647
Size: 997
Fragments arranged by size:
3064 997 979 717 604 518 438 287
43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2083 2293 4834 7647
Size: 2083 210 2541 2813
Fragments arranged by size:
2813 2541 2083 210
EcoICRI GAG'CTC
Cuts at: 0 129 2568 4008 7647
Size: 129 2439 1440 3639
Fragments arranged by size:
3639 2439 1440 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1534 2347 2467 2635 3395 3886 3895
Size: 115 1419 813 120 168 760 491 9
Cuts at: 3895 4073 4225 4348 4816 5683 6004 6172 6214
Size: 178 152 123 468 867 321 168 42
Cuts at: 6214 7305 7647
Size: 1091 342
Fragments arranged by size:
1419 1091 867 813 760 491 468 342
321 178 168 168 152 123 120 115
42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1720 2062 2971 3796 3919 6094 7647
Size: 1720 342 909 825 123 2175 1553
Fragments arranged by size:
2175 1720 1553 909 825 342 123
EcoO109I rG'GnC_Cy
Cuts at: 0 597 1037 1677 3757 3912 4107 4108 4219
Size: 597 440 640 2080 155 195 1 111
Cuts at: 4219 4662 4704 5910 6180 6181 6285 6303 6402
Size: 443 42 1206 270 1 104 18 99
Cuts at: 6402 6503 7473 7647
Size: 101 970 174
Fragments arranged by size:
2080 1206 970 640 597 443 440 270
195 174 155 111 104 101 99 42
18 1 1
EcoRI G'AATT_C
Cuts at: 0 3118 4084 7647
Size: 3118 966 3563
Fragments arranged by size:
3563 3118 966
EcoRV GAT'ATC
Cuts at: 0 1500 7647
Size: 1500 6147
FspI TGC'GCA
Cuts at: 0 2191 7647
Size: 2191 5456
HaeII r_GCGC'y
Cuts at: 0 2046 3314 3406 3624 3812 5333 7647
Size: 2046 1268 92 218 188 1521 2314
Fragments arranged by size:
2314 2046 1521 1268 218 188 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1980 2012 2636 2668 3439 3471 3514 3546
Size: 1980 32 624 32 771 32 43 32
Cuts at: 3546 3593 3625 3871 3903 4654 4686 4734 4766
Size: 47 32 246 32 751 32 48 32
Cuts at: 4766 4823 4826 4855 4858 5602 5634 6044 6076
Size: 57 3 29 3 744 32 410 32
Cuts at: 6076 6625 6657 6662 6694 7330 7362 7647
Size: 549 32 5 32 636 32 285
Fragments arranged by size:
1980 771 751 744 636 624 549 410
285 246 57 48 47 43 32 32
32 32 32 32 32 32 32 32
32 32 32 29 5 3 3
HincII GTy'rAC
Cuts at: 0 2808 4474 6615 7148 7647
Size: 2808 1666 2141 533 499
Fragments arranged by size:
2808 2141 1666 533 499
HpaI GTT'AAC
Cuts at: 0 6615 7647
Size: 6615 1032
KasI G'GCGC_C
Cuts at: 0 3310 7647
Size: 3310 4337
KpnI G_GTAC'C
Cuts at: 0 5901 7647
Size: 5901 1746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1230 2201 6348 7049 7588 7647
Size: 1230 971 4147 701 539 59
Fragments arranged by size:
4147 1230 971 701 539 59
MscI TGG'CCA
Cuts at: 0 265 526 748 1916 2131 2503 2545 3719
Size: 265 261 222 1168 215 372 42 1174
Cuts at: 3719 3940 5748 6058 6628 6898 7355 7647
Size: 221 1808 310 570 270 457 292
Fragments arranged by size:
1808 1174 1168 570 457 372 310 292
270 265 261 222 221 215 42
MslI CAynn'nnrTG
Cuts at: 0 283 370 583 1066 1459 1555 1922 2476
Size: 283 87 213 483 393 96 367 554
Cuts at: 2476 2656 2710 3109 3517 3577 4600 4683 6031
Size: 180 54 399 408 60 1023 83 1348
Cuts at: 6031 6982 7207 7246 7647
Size: 951 225 39 401
Fragments arranged by size:
1348 1023 951 554 483 408 401 399
393 367 283 225 213 180 96 87
83 60 54 39
MspA1I CmG'CkG
Cuts at: 0 34 424 502 879 1294 1692 2845 3045
Size: 34 390 78 377 415 398 1153 200
Cuts at: 3045 3219 3303 3549 4285 4555 5274 5354 5462
Size: 174 84 246 736 270 719 80 108
Cuts at: 5462 5592 6342 6883 7647
Size: 130 750 541 764
Fragments arranged by size:
1153 764 750 736 719 541 415 398
390 377 270 246 200 174 130 108
84 80 78 34
NarI GG'CG_CC
Cuts at: 0 3311 7647
Size: 3311 4336
NcoI C'CATG_G
Cuts at: 0 146 401 783 2852 2906 3518 4766 7647
Size: 146 255 382 2069 54 612 1248 2881
Fragments arranged by size:
2881 2069 1248 612 382 255 146 54
NdeI CA'TA_TG
Cuts at: 0 4176 4758 6429 7647
Size: 4176 582 1671 1218
Fragments arranged by size:
4176 1671 1218 582
NheI G'CTAG_C
Cuts at: 0 271 7647
Size: 271 7376
NsiI A_TGCA'T
Cuts at: 0 3251 3732 7647
Size: 3251 481 3915
Fragments arranged by size:
3915 3251 481
NspI r_CATG'y
Cuts at: 0 499 1464 1671 2268 2481 3234 4443 4785
Size: 499 965 207 597 213 753 1209 342
Cuts at: 4785 5112 5151 5191 5374 5994 6141 6166 6868
Size: 327 39 40 183 620 147 25 702
Cuts at: 6868 7199 7647
Size: 331 448
Fragments arranged by size:
1209 965 753 702 620 597 499 448
342 331 327 213 207 183 147 40
39 25
PasI CC'CwG_GG
Cuts at: 0 1308 2529 2943 7647
Size: 1308 1221 414 4704
Fragments arranged by size:
4704 1308 1221 414
PciI A'CATG_T
Cuts at: 0 5108 5990 7647
Size: 5108 882 1657
Fragments arranged by size:
5108 1657 882
PflMI CCAn_nnn'nTGG
Cuts at: 0 1308 1692 3045 6065 7647
Size: 1308 384 1353 3020 1582
Fragments arranged by size:
3020 1582 1353 1308 384
PfoI T'CCnGG_A
Cuts at: 0 2120 2483 3023 3457 5699 6749 7647
Size: 2120 363 540 434 2242 1050 898
Fragments arranged by size:
2242 2120 1050 898 540 434 363
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 912 944 7647
Size: 912 32 6703
Fragments arranged by size:
6703 912 32
PpuMI rG'GwC_Cy
Cuts at: 0 597 1037 3757 4662 4704 6285 6303 6402
Size: 597 440 2720 905 42 1581 18 99
Cuts at: 6402 7647
Size: 1245
Fragments arranged by size:
2720 1581 1245 905 597 440 99 42
18
PshAI GACnn'nnGTC
Cuts at: 0 5891 7647
Size: 5891 1756
PspOMI G'GGCC_C
Cuts at: 0 3911 4107 6180 7647
Size: 3911 196 2073 1467
Fragments arranged by size:
3911 2073 1467 196
PspXI vC'TCGA_Gb
Cuts at: 0 1469 4450 7647
Size: 1469 2981 3197
Fragments arranged by size:
3197 2981 1469
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6942 6974 7647
Size: 6942 32 673
Fragments arranged by size:
6942 673 32
PstI C_TGCA'G
Cuts at: 0 68 1557 3643 4554 4871 5321 5597 7647
Size: 68 1489 2086 911 317 450 276 2050
Fragments arranged by size:
2086 2050 1489 911 450 317 276 68
PvuII CAG'CTG
Cuts at: 0 502 879 1692 2845 3045 3219 3303 3549
Size: 502 377 813 1153 200 174 84 246
Cuts at: 3549 4555 5274 5354 6342 6883 7647
Size: 1006 719 80 988 541 764
Fragments arranged by size:
1153 1006 988 813 764 719 541 502
377 246 200 174 84 80
SacI G_AGCT'C
Cuts at: 0 131 2570 4010 7647
Size: 131 2439 1440 3637
Fragments arranged by size:
3637 2439 1440 131
SanDI GG'GwC_CC
Cuts at: 0 597 7647
Size: 597 7050
SapI GCTCTTCn'nnn_
Cuts at: 0 3064 6607 7647
Size: 3064 3543 1040
Fragments arranged by size:
3543 3064 1040
ScaI AGT'ACT
Cuts at: 0 895 5323 7647
Size: 895 4428 2324
Fragments arranged by size:
4428 2324 895
SexAI A'CCwGG_T
Cuts at: 0 2105 2276 4342 7647
Size: 2105 171 2066 3305
Fragments arranged by size:
3305 2105 2066 171
SfcI C'TryA_G
Cuts at: 0 64 626 1553 2571 2784 3639 4267 4550
Size: 64 562 927 1018 213 855 628 283
Cuts at: 4550 4867 5281 5317 5325 5593 5806 6756 7185
Size: 317 414 36 8 268 213 950 429
Cuts at: 7185 7647
Size: 462
Fragments arranged by size:
1018 950 927 855 628 562 462 429
414 317 283 268 213 213 64 36
8
SfoI GGC'GCC
Cuts at: 0 3312 7647
Size: 3312 4335
SmaI CCC'GGG
Cuts at: 0 2728 7647
Size: 2728 4919
SmlI C'TyrA_G
Cuts at: 0 1469 2593 4450 4960 7647
Size: 1469 1124 1857 510 2687
Fragments arranged by size:
2687 1857 1469 1124 510
SpeI A'CTAG_T
Cuts at: 0 2791 7647
Size: 2791 4856
SphI G_CATG'C
Cuts at: 0 1464 1671 2268 4443 5191 5374 6166 6868
Size: 1464 207 597 2175 748 183 792 702
Cuts at: 6868 7647
Size: 779
Fragments arranged by size:
2175 1464 792 779 748 702 597 207
183
StuI AGG'CCT
Cuts at: 0 1022 2083 3802 3925 4456 5365 5764 6089
Size: 1022 1061 1719 123 531 909 399 325
Cuts at: 6089 6217 7004 7172 7647
Size: 128 787 168 475
Fragments arranged by size:
1719 1061 1022 909 787 531 475 399
325 168 128 123
StyI C'CwwG_G
Cuts at: 0 122 146 380 401 743 783 986 1017
Size: 122 24 234 21 342 40 203 31
Cuts at: 1017 1673 2852 2906 3518 3720 4457 4673 4766
Size: 656 1179 54 612 202 737 216 93
Cuts at: 4766 5852 7647
Size: 1086 1795
Fragments arranged by size:
1795 1179 1086 737 656 612 342 234
216 203 202 122 93 54 40 31
24 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 1116 1676 4138 4633 6307 6959 7034
Size: 457 659 560 2462 495 1674 652 75
Cuts at: 7034 7647
Size: 613
Fragments arranged by size:
2462 1674 659 652 613 560 495 457
75
TatI w'GTAC_w
Cuts at: 0 893 963 4811 5321 5636 6110 6260 7647
Size: 893 70 3848 510 315 474 150 1387
Fragments arranged by size:
3848 1387 893 510 474 315 150 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1063 1095 1883 1915 2266 2298 2525 2557
Size: 1063 32 788 32 351 32 227 32
Cuts at: 2557 3041 3073 4771 4803 7647
Size: 484 32 1698 32 2844
Fragments arranged by size:
2844 1698 1063 788 484 351 227 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3429 7237 7647
Size: 3429 3808 410
Fragments arranged by size:
3808 3429 410
XbaI T'CTAG_A
Cuts at: 0 4236 7647
Size: 4236 3411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1527 1909 2139 2511 4707 5853 7032 7624
Size: 1527 382 230 372 2196 1146 1179 592
Cuts at: 7624 7647
Size: 23
Fragments arranged by size:
2196 1527 1179 1146 592 382 372 230
23
XhoI C'TCGA_G
Cuts at: 0 1469 4450 7647
Size: 1469 2981 3197
Fragments arranged by size:
3197 2981 1469
XmaI C'CCGG_G
Cuts at: 0 2726 7647
Size: 2726 4921
XmnI GAAnn'nnTTC
Cuts at: 0 7433 7647
Size: 7433 214
ZraI GAC'GTC
Cuts at: 0 5863 7647
Size: 5863 1784
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AseI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HpaI KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NsiI NspI PasI PciI PflMI PfoI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI ApaLI AscI AsiSI AvrII BaeI
BsiWI BssHII BstZ17I DraI FalI FseI FspAI HindIII
MfeI MluI NaeI NgoMIV NotI NruI PacI PmeI
PmlI PsiI PvuI RsrII SacII SalI SbfI SfiI
SgfI SgrAI SnaBI SrfI SspI SwaI