Restriction map for
pF1KB1311
(Linear) (Six-base) MAPSORT of: KIBB1311.seq Check: 5897 from: 1 to: 4152
KIBB1311.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 701 4152
Size: 701 3451
AccI GT'mk_AC
Cuts at: 0 3227 4152
Size: 3227 925
Acc65I G'GTAC_C
Cuts at: 0 1074 1307 2854 3167 4152
Size: 1074 233 1547 313 985
Fragments arranged by size:
1547 1074 985 313 233
AclI AA'CG_TT
Cuts at: 0 3233 4152
Size: 3233 919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 1276 1843 2421 3240 3246 3337 3992
Size: 585 691 567 578 819 6 91 655
Cuts at: 3992 4152
Size: 160
Fragments arranged by size:
819 691 655 585 578 567 160 91
6
AfeI AGC'GCT
Cuts at: 0 853 2140 2929 3047 3598 4152
Size: 853 1287 789 118 551 554
Fragments arranged by size:
1287 853 789 554 551 118
AflII C'TTAA_G
Cuts at: 0 2764 4152
Size: 2764 1388
AflIII A'CryG_T
Cuts at: 0 292 590 1259 1609 1640 2942 2944 3386
Size: 292 298 669 350 31 1302 2 442
Cuts at: 3386 4152
Size: 766
Fragments arranged by size:
1302 766 669 442 350 298 292 31
2
AleI CACnn'nnGTG
Cuts at: 0 751 4152
Size: 751 3401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1540 3040 4152
Size: 1540 1500 1112
Fragments arranged by size:
1540 1500 1112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2347 2379 4152
Size: 2347 32 1773
Fragments arranged by size:
2347 1773 32
AlwNI CAG_nnn'CTG
Cuts at: 0 4015 4099 4131 4152
Size: 4015 84 32 21
Fragments arranged by size:
4015 84 32 21
ApaI G_GGCC'C
Cuts at: 0 2485 4152
Size: 2485 1667
ApoI r'AATT_y
Cuts at: 0 825 1341 2346 4152
Size: 825 516 1005 1806
Fragments arranged by size:
1806 1005 825 516
AvaI C'yCGr_G
Cuts at: 0 673 1901 2454 2534 3120 3163 4106 4152
Size: 673 1228 553 80 586 43 943 46
Fragments arranged by size:
1228 943 673 586 553 80 46 43
BamHI G'GATC_C
Cuts at: 0 198 242 2132 4152
Size: 198 44 1890 2020
Fragments arranged by size:
2020 1890 198 44
BanI G'GyrC_C
Cuts at: 0 115 649 922 1019 1074 1307 2498 2624
Size: 115 534 273 97 55 233 1191 126
Cuts at: 2624 2854 3016 3167 3373 3777 4152
Size: 230 162 151 206 404 375
Fragments arranged by size:
1191 534 404 375 273 233 230 206
162 151 126 115 97 55
BanII G_rGCy'C
Cuts at: 0 268 2485 3940 3959 4152
Size: 268 2217 1455 19 193
Fragments arranged by size:
2217 1455 268 193 19
BbeI G_GCGC'C
Cuts at: 0 119 653 926 1023 2502 4152
Size: 119 534 273 97 1479 1650
Fragments arranged by size:
1650 1479 534 273 119 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 622 2454 2538 4152
Size: 622 1832 84 1614
Fragments arranged by size:
1832 1614 622 84
BbvCI CC'TCA_GC
Cuts at: 0 1592 3707 4062 4152
Size: 1592 2115 355 90
Fragments arranged by size:
2115 1592 355 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 12 46 1466 1500 3907 3941 4152
Size: 12 34 1420 34 2407 34 211
Fragments arranged by size:
2407 1420 211 34 34 34 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 2274 4152
Size: 2274 1878
BfrBI ATG'CAT
Cuts at: 0 6 1960 4152
Size: 6 1954 2192
Fragments arranged by size:
2192 1954 6
BglI GCCn_nnn'nGGC
Cuts at: 0 497 3198 4152
Size: 497 2701 954
Fragments arranged by size:
2701 954 497
BlpI GC'TnA_GC
Cuts at: 0 1240 3082 3698 4152
Size: 1240 1842 616 454
Fragments arranged by size:
1842 1240 616 454
Bme1580I G_kGCm'C
Cuts at: 0 356 449 690 1124 1983 2485 2886 3019
Size: 356 93 241 434 859 502 401 133
Cuts at: 3019 3209 3376 4152
Size: 190 167 776
Fragments arranged by size:
859 776 502 434 401 356 241 190
167 133 93
BmgBI CAC'GTC
Cuts at: 0 591 3763 4152
Size: 591 3172 389
Fragments arranged by size:
3172 591 389
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 2121 2205 2301 4152
Size: 294 1827 84 96 1851
Fragments arranged by size:
1851 1827 294 96 84
BmtI G_CTAG'C
Cuts at: 0 3706 4152
Size: 3706 446
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2874 3267 4152
Size: 2874 393 885
Fragments arranged by size:
2874 885 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 700 2848 4040 4070 4152
Size: 199 501 2148 1192 30 82
Fragments arranged by size:
2148 1192 501 199 82 30
Bpu10I CC'TnA_GC
Cuts at: 0 1592 3707 3785 4062 4152
Size: 1592 2115 78 277 90
Fragments arranged by size:
2115 1592 277 90 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1513 2811 3646 4152
Size: 1513 1298 835 506
Fragments arranged by size:
1513 1298 835 506
BsaI GGTCTCn'nnnn_
Cuts at: 0 45 1413 1714 3255 4152
Size: 45 1368 301 1541 897
Fragments arranged by size:
1541 1368 897 301 45
BsaAI yAC'GTr
Cuts at: 0 666 1227 2945 3387 4152
Size: 666 561 1718 442 765
Fragments arranged by size:
1718 765 666 561 442
BsaBI GATnn'nnATC
Cuts at: 0 203 904 4152
Size: 203 701 3248
Fragments arranged by size:
3248 701 203
BsaHI Gr'CG_yC
Cuts at: 0 116 650 923 1020 2499 2772 4152
Size: 116 534 273 97 1479 273 1380
Fragments arranged by size:
1479 1380 534 273 273 116 97
BsaWI w'CCGG_w
Cuts at: 0 239 1427 2159 3455 4152
Size: 239 1188 732 1296 697
Fragments arranged by size:
1296 1188 732 697 239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 902 932 1474 1504 2433 2463 3233 3263
Size: 902 30 542 30 929 30 770 30
Cuts at: 3263 4026 4056 4129 4152
Size: 763 30 73 23
Fragments arranged by size:
929 902 770 763 542 73 30 30
30 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 111 631 2110 3259 4150 4152
Size: 94 17 520 1479 1149 891 2
Fragments arranged by size:
1479 1149 891 520 94 17 2
BseYI C'CCAG_C
Cuts at: 0 56 1218 1303 1997 2123 3369 3882 4068
Size: 56 1162 85 694 126 1246 513 186
Cuts at: 4068 4074 4092 4152
Size: 6 18 60
Fragments arranged by size:
1246 1162 694 513 186 126 85 60
56 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 394 622 685 868 1650 2366 2853
Size: 148 246 228 63 183 782 716 487
Cuts at: 2853 4152
Size: 1299
Fragments arranged by size:
1299 782 716 487 246 228 183 148
63
BsiEI CG_ry'CG
Cuts at: 0 318 1281 2343 4152
Size: 318 963 1062 1809
Fragments arranged by size:
1809 1062 963 318
BsiHKAI G_wGCw'C
Cuts at: 0 3332 3908 4152
Size: 3332 576 244
Fragments arranged by size:
3332 576 244
BsmI GAATG_Cn'
Cuts at: 0 1504 1962 4152
Size: 1504 458 2190
Fragments arranged by size:
2190 1504 458
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2763 4152
Size: 2763 1389
Bsp1286I G_dGCh'C
Cuts at: 0 268 356 449 690 1124 1983 2485 2886
Size: 268 88 93 241 434 859 502 401
Cuts at: 2886 3019 3209 3332 3376 3908 3940 3959 4152
Size: 133 190 123 44 532 32 19 193
Fragments arranged by size:
859 532 502 434 401 268 241 193
190 133 123 93 88 44 32 19
BspEI T'CCGG_A
Cuts at: 0 1427 4152
Size: 1427 2725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 416 701 1242 1732 4152
Size: 416 285 541 490 2420
Fragments arranged by size:
2420 541 490 416 285
BsrBI CCG'CTC
Cuts at: 0 1277 1926 2291 4152
Size: 1277 649 365 1861
Fragments arranged by size:
1861 1277 649 365
BsrFI r'CCGG_y
Cuts at: 0 1571 2678 3086 4152
Size: 1571 1107 408 1066
Fragments arranged by size:
1571 1107 1066 408
BssHII G'CGCG_C
Cuts at: 0 427 4152
Size: 427 3725
BssSI C'ACGA_G
Cuts at: 0 563 1333 3970 4152
Size: 563 770 2637 182
Fragments arranged by size:
2637 770 563 182
BstAPI GCAn_nnn'nTGC
Cuts at: 0 795 4152
Size: 795 3357
BstEII G'GTnAC_C
Cuts at: 0 182 3211 4152
Size: 182 3029 941
Fragments arranged by size:
3029 941 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2995 4152
Size: 2995 1157
BstYI r'GATC_y
Cuts at: 0 198 242 2132 2752 3590 4127 4152
Size: 198 44 1890 620 838 537 25
Fragments arranged by size:
1890 838 620 537 198 44 25
Bsu36I CC'TnA_GG
Cuts at: 0 481 2317 4152
Size: 481 1836 1835
Fragments arranged by size:
1836 1835 481
BtgI C'CryG_G
Cuts at: 0 35 187 251 960 1137 1509 2907 3156
Size: 35 152 64 709 177 372 1398 249
Cuts at: 3156 3216 4152
Size: 60 936
Fragments arranged by size:
1398 936 709 372 249 177 152 64
60 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 833 984 1251 3910 4152
Size: 833 151 267 2659 242
Fragments arranged by size:
2659 833 267 242 151
BtsI GCAGTG_nn'
Cuts at: 0 422 1818 2051 2704 4152
Size: 422 1396 233 653 1448
Fragments arranged by size:
1448 1396 653 422 233
DraIII CAC_nnn'GTG
Cuts at: 0 795 1138 1338 4152
Size: 795 343 200 2814
Fragments arranged by size:
2814 795 343 200
EaeI y'GGCC_r
Cuts at: 0 254 813 1010 1140 1278 1927 2085 3088
Size: 254 559 197 130 138 649 158 1003
Cuts at: 3088 3420 4152
Size: 332 732
Fragments arranged by size:
1003 732 649 559 332 254 197 158
138 130
EagI C'GGCC_G
Cuts at: 0 1278 4152
Size: 1278 2874
EarI CTCTTCn'nnn_
Cuts at: 0 22 3469 3913 4152
Size: 22 3447 444 239
Fragments arranged by size:
3447 444 239 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 779 3264 4152
Size: 779 2485 888
Fragments arranged by size:
2485 888 779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 585 700 1276 1843 2421 2848 3240
Size: 199 386 115 576 567 578 427 392
Cuts at: 3240 3246 3337 3992 4040 4070 4152
Size: 6 91 655 48 30 82
Fragments arranged by size:
655 578 576 567 427 392 386 199
115 91 82 48 30 6
EcoO109I rG'GnC_Cy
Cuts at: 0 91 1099 1176 1436 1596 2441 2481 2482
Size: 91 1008 77 260 160 845 40 1
Cuts at: 2482 3347 3711 4012 4023 4152
Size: 865 364 301 11 129
Fragments arranged by size:
1008 865 845 364 301 260 160 129
91 77 40 11 1
EcoRV GAT'ATC
Cuts at: 0 1848 3364 4152
Size: 1848 1516 788
Fragments arranged by size:
1848 1516 788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3687 4152
Size: 3687 465
HaeII r_GCGC'y
Cuts at: 0 119 653 855 926 1023 2142 2502 2931
Size: 119 534 202 71 97 1119 360 429
Cuts at: 2931 3049 3600 4152
Size: 118 551 552
Fragments arranged by size:
1119 552 551 534 429 360 202 119
118 97 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 532 564 1474 1506 2433 2465
Size: 183 32 317 32 910 32 927 32
Cuts at: 2465 3233 3265 4129 4152
Size: 768 32 864 23
Fragments arranged by size:
927 910 864 768 317 183 32 32
32 32 32 23
HincII GTy'rAC
Cuts at: 0 996 1257 1543 1551 4152
Size: 996 261 286 8 2601
Fragments arranged by size:
2601 996 286 261 8
HindIII A'AGCT_T
Cuts at: 0 1288 4152
Size: 1288 2864
KasI G'GCGC_C
Cuts at: 0 115 649 922 1019 2498 4152
Size: 115 534 273 97 1479 1654
Fragments arranged by size:
1654 1479 534 273 115 97
KpnI G_GTAC'C
Cuts at: 0 1078 1311 2858 3171 4152
Size: 1078 233 1547 313 981
Fragments arranged by size:
1547 1078 981 313 233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1517 3509 4152
Size: 1517 1992 643
Fragments arranged by size:
1992 1517 643
MscI TGG'CCA
Cuts at: 0 256 3422 4152
Size: 256 3166 730
Fragments arranged by size:
3166 730 256
MslI CAynn'nnrTG
Cuts at: 0 751 898 2722 3022 3325 3472 3950 4152
Size: 751 147 1824 300 303 147 478 202
Fragments arranged by size:
1824 751 478 303 300 202 147 147
MspA1I CmG'CkG
Cuts at: 0 37 329 962 2918 3357 3369 4152
Size: 37 292 633 1956 439 12 783
Fragments arranged by size:
1956 783 633 439 292 37 12
NaeI GCC'GGC
Cuts at: 0 1573 3088 4152
Size: 1573 1515 1064
Fragments arranged by size:
1573 1515 1064
NarI GG'CG_CC
Cuts at: 0 116 650 923 1020 2499 4152
Size: 116 534 273 97 1479 1653
Fragments arranged by size:
1653 1479 534 273 116 97
NcoI C'CATG_G
Cuts at: 0 1509 2907 3156 4152
Size: 1509 1398 249 996
Fragments arranged by size:
1509 1398 996 249
NgoMIV G'CCGG_C
Cuts at: 0 1571 3086 4152
Size: 1571 1515 1066
Fragments arranged by size:
1571 1515 1066
NheI G'CTAG_C
Cuts at: 0 3702 4152
Size: 3702 450
NsiI A_TGCA'T
Cuts at: 0 8 1962 4152
Size: 8 1954 2190
Fragments arranged by size:
2190 1954 8
NspI r_CATG'y
Cuts at: 0 303 1613 1644 3081 4152
Size: 303 1310 31 1437 1071
Fragments arranged by size:
1437 1310 1071 303 31
PasI CC'CwG_GG
Cuts at: 0 111 654 2684 4152
Size: 111 543 2030 1468
Fragments arranged by size:
2030 1468 543 111
PciI A'CATG_T
Cuts at: 0 1609 1640 4152
Size: 1609 31 2512
Fragments arranged by size:
2512 1609 31
PflMI CCAn_nnn'nTGG
Cuts at: 0 193 1138 1478 2418 4099 4131 4152
Size: 193 945 340 940 1681 32 21
Fragments arranged by size:
1681 945 940 340 193 32 21
PfoI T'CCnGG_A
Cuts at: 0 1027 2350 4130 4152
Size: 1027 1323 1780 22
Fragments arranged by size:
1780 1323 1027 22
PmlI CAC'GTG
Cuts at: 0 1227 2945 4152
Size: 1227 1718 1207
Fragments arranged by size:
1718 1227 1207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2293 2325 3487 3519 4152
Size: 2293 32 1162 32 633
Fragments arranged by size:
2293 1162 633 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 91 1176 1436 1596 2441 3347 4023 4152
Size: 91 1085 260 160 845 906 676 129
Fragments arranged by size:
1085 906 845 676 260 160 129 91
PshAI GACnn'nnGTC
Cuts at: 0 3574 4152
Size: 3574 578
PspOMI G'GGCC_C
Cuts at: 0 2481 4152
Size: 2481 1671
PspXI vC'TCGA_Gb
Cuts at: 0 673 1901 4152
Size: 673 1228 2251
Fragments arranged by size:
2251 1228 673
PstI C_TGCA'G
Cuts at: 0 2058 2711 2760 3060 4152
Size: 2058 653 49 300 1092
Fragments arranged by size:
2058 1092 653 300 49
PvuI CG_AT'CG
Cuts at: 0 2343 4152
Size: 2343 1809
PvuII CAG'CTG
Cuts at: 0 329 3357 3369 4152
Size: 329 3028 12 783
Fragments arranged by size:
3028 783 329 12
SacII CC_GC'GG
Cuts at: 0 38 963 4152
Size: 38 925 3189
Fragments arranged by size:
3189 925 38
SanDI GG'GwC_CC
Cuts at: 0 91 3347 4152
Size: 91 3256 805
Fragments arranged by size:
3256 805 91
SapI GCTCTTCn'nnn_
Cuts at: 0 22 3913 4152
Size: 22 3891 239
Fragments arranged by size:
3891 239 22
ScaI AGT'ACT
Cuts at: 0 1951 4152
Size: 1951 2201
SexAI A'CCwGG_T
Cuts at: 0 738 2627 4152
Size: 738 1889 1525
Fragments arranged by size:
1889 1525 738
SfcI C'TryA_G
Cuts at: 0 129 2054 2070 2707 2756 2931 3037 3056
Size: 129 1925 16 637 49 175 106 19
Cuts at: 3056 4034 4152
Size: 978 118
Fragments arranged by size:
1925 978 637 175 129 118 106 49
19 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3198 4152
Size: 3198 954
SfoI GGC'GCC
Cuts at: 0 117 651 924 1021 2500 4152
Size: 117 534 273 97 1479 1652
Fragments arranged by size:
1652 1479 534 273 117 97
SmaI CCC'GGG
Cuts at: 0 2536 3122 3165 4108 4152
Size: 2536 586 43 943 44
Fragments arranged by size:
2536 943 586 44 43
SmlI C'TyrA_G
Cuts at: 0 673 1528 1901 2764 2790 3661 4152
Size: 673 855 373 863 26 871 491
Fragments arranged by size:
871 863 855 673 491 373 26
StuI AGG'CCT
Cuts at: 0 2322 3544 4152
Size: 2322 1222 608
Fragments arranged by size:
2322 1222 608
StyI C'CwwG_G
Cuts at: 0 1509 2907 3156 3539 3959 4152
Size: 1509 1398 249 383 420 193
Fragments arranged by size:
1509 1398 420 383 249 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 332 628 821 2877 3430 3552 4152
Size: 332 296 193 2056 553 122 600
Fragments arranged by size:
2056 600 553 332 296 193 122
TatI w'GTAC_w
Cuts at: 0 1838 1949 2183 2586 4152
Size: 1838 111 234 403 1566
Fragments arranged by size:
1838 1566 403 234 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 902 934 1785 1817 2375 2407 3887 3919
Size: 902 32 851 32 558 32 1480 32
Cuts at: 3919 4152
Size: 233
Fragments arranged by size:
1480 902 851 558 233 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 286 740 4152
Size: 286 454 3412
Fragments arranged by size:
3412 454 286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1475 2415 2995 4152
Size: 1475 940 580 1157
Fragments arranged by size:
1475 1157 940 580
XhoI C'TCGA_G
Cuts at: 0 673 1901 4152
Size: 673 1228 2251
Fragments arranged by size:
2251 1228 673
XmaI C'CCGG_G
Cuts at: 0 2534 3120 3163 4106 4152
Size: 2534 586 43 943 46
Fragments arranged by size:
2534 943 586 46 43
XmnI GAAnn'nnTTC
Cuts at: 0 3463 4152
Size: 3463 689
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII SacII SanDI SapI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AgeI AhdI ApaLI AscI AseI AsiSI AvrII
BaeI BclI BglII BsiWI BspHI BsrDI BsrGI BstBI
BstZ17I ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI
FseI FspI FspAI HpaI MfeI MluI NdeI NotI
NruI PacI PmeI PsiI PsrI RsrII SacI SalI
SbfI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI ZraI