Restriction map for
pF1KSDB1316
(Linear) (Six-base) MAPSORT of: KIBB1316.seq Check: 1524 from: 1 to: 2586
KIBB1316.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1531 2586
Size: 1531 1055
AatII G_ACGT'C
Cuts at: 0 532 2543 2586
Size: 532 2011 43
Fragments arranged by size:
2011 532 43
AccI GT'mk_AC
Cuts at: 0 692 2586
Size: 692 1894
Acc65I G'GTAC_C
Cuts at: 0 1083 2586
Size: 1083 1503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 2386 2575 2586
Size: 931 1455 189 11
Fragments arranged by size:
1455 931 189 11
AflII C'TTAA_G
Cuts at: 0 2182 2586
Size: 2182 404
AflIII A'CryG_T
Cuts at: 0 371 2586
Size: 371 2215
AleI CACnn'nnGTG
Cuts at: 0 709 1039 2011 2586
Size: 709 330 972 575
Fragments arranged by size:
972 709 575 330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 783 2586
Size: 783 1803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 406 438 2586
Size: 406 32 2148
Fragments arranged by size:
2148 406 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1290 1425 1872 2364 2586
Size: 1290 135 447 492 222
Fragments arranged by size:
1290 492 447 222 135
ApaLI G'TGCA_C
Cuts at: 0 9 109 975 2586
Size: 9 100 866 1611
Fragments arranged by size:
1611 866 100 9
ApoI r'AATT_y
Cuts at: 0 570 1152 2296 2586
Size: 570 582 1144 290
Fragments arranged by size:
1144 582 570 290
AvaI C'yCGr_G
Cuts at: 0 197 903 1360 1476 1748 2586
Size: 197 706 457 116 272 838
Fragments arranged by size:
838 706 457 272 197 116
BanI G'GyrC_C
Cuts at: 0 193 915 1083 1519 2586
Size: 193 722 168 436 1067
Fragments arranged by size:
1067 722 436 193 168
BanII G_rGCy'C
Cuts at: 0 1341 1570 1612 1795 2586
Size: 1341 229 42 183 791
Fragments arranged by size:
1341 791 229 183 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 278 858 879 999 1427 1896 2030
Size: 201 77 580 21 120 428 469 134
Cuts at: 2030 2586
Size: 556
Fragments arranged by size:
580 556 469 428 201 134 120 77
21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 821 855 2500 2534 2586
Size: 821 34 1645 34 52
Fragments arranged by size:
1645 821 52 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2266 2586
Size: 2266 320
BglI GCCn_nnn'nGGC
Cuts at: 0 202 674 1498 2062 2586
Size: 202 472 824 564 524
Fragments arranged by size:
824 564 524 472 202
BlpI GC'TnA_GC
Cuts at: 0 163 2586
Size: 163 2423
Bme1580I G_kGCm'C
Cuts at: 0 13 113 198 979 1660 2586
Size: 13 100 85 781 681 926
Fragments arranged by size:
926 781 681 100 85 13
BmgBI CAC'GTC
Cuts at: 0 645 1926 2586
Size: 645 1281 660
Fragments arranged by size:
1281 660 645
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1026 2586
Size: 1026 1560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 1584 2586
Size: 1121 463 1002
Fragments arranged by size:
1121 1002 463
Bpu10I CC'TnA_GC
Cuts at: 0 895 2586
Size: 895 1691
BsaI GGTCTCn'nnnn_
Cuts at: 0 270 1381 2440 2586
Size: 270 1111 1059 146
Fragments arranged by size:
1111 1059 270 146
BsaAI yAC'GTr
Cuts at: 0 372 2586
Size: 372 2214
BsaBI GATnn'nnATC
Cuts at: 0 2137 2586
Size: 2137 449
BsaHI Gr'CG_yC
Cuts at: 0 529 2540 2586
Size: 529 2011 46
Fragments arranged by size:
2011 529 46
BsaWI w'CCGG_w
Cuts at: 0 1277 2586
Size: 1277 1309
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 712 742 1129 1159 2076 2106 2586
Size: 712 30 387 30 917 30 480
Fragments arranged by size:
917 712 480 387 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1015 1399 1894 2061 2194 2586
Size: 1015 384 495 167 133 392
Fragments arranged by size:
1015 495 392 384 167 133
BseYI C'CCAG_C
Cuts at: 0 477 1391 1612 2052 2153 2399 2586
Size: 477 914 221 440 101 246 187
Fragments arranged by size:
914 477 440 246 221 187 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 311 1327 1579 2080 2586
Size: 139 172 1016 252 501 506
Fragments arranged by size:
1016 506 501 252 172 139
BsiHKAI G_wGCw'C
Cuts at: 0 13 101 113 896 979 1036 1341 1367
Size: 13 88 12 783 83 57 305 26
Cuts at: 1367 2417 2586
Size: 1050 169
Fragments arranged by size:
1050 783 305 169 88 83 57 26
13 12
BsmI GAATG_Cn'
Cuts at: 0 388 2586
Size: 388 2198
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2365 2586
Size: 2365 221
Bsp1286I G_dGCh'C
Cuts at: 0 13 101 113 198 896 979 1036 1341
Size: 13 88 12 85 698 83 57 305
Cuts at: 1341 1367 1570 1612 1660 1795 2417 2586
Size: 26 203 42 48 135 622 169
Fragments arranged by size:
698 622 305 203 169 135 88 85
83 57 48 42 26 13 12
BspHI T'CATG_A
Cuts at: 0 126 2586
Size: 126 2460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1531 1591 2285 2586
Size: 1531 60 694 301
Fragments arranged by size:
1531 694 301 60
BsrBI CCG'CTC
Cuts at: 0 1640 2303 2586
Size: 1640 663 283
Fragments arranged by size:
1640 663 283
BsrFI r'CCGG_y
Cuts at: 0 396 1682 2110 2437 2586
Size: 396 1286 428 327 149
Fragments arranged by size:
1286 428 396 327 149
BsrGI T'GTAC_A
Cuts at: 0 185 2586
Size: 185 2401
BstAPI GCAn_nnn'nTGC
Cuts at: 0 117 289 2120 2586
Size: 117 172 1831 466
Fragments arranged by size:
1831 466 172 117
BstEII G'GTnAC_C
Cuts at: 0 1209 2586
Size: 1209 1377
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2016 2407 2586
Size: 2016 391 179
Fragments arranged by size:
2016 391 179
Bsu36I CC'TnA_GG
Cuts at: 0 790 2586
Size: 790 1796
BtgI C'CryG_G
Cuts at: 0 751 1014 1093 2480 2586
Size: 751 263 79 1387 106
Fragments arranged by size:
1387 751 263 106 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 76 1658 2586
Size: 76 1582 928
Fragments arranged by size:
1582 928 76
BtsI GCAGTG_nn'
Cuts at: 0 50 977 2586
Size: 50 927 1609
Fragments arranged by size:
1609 927 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1686 1721 2586
Size: 1686 35 865
Fragments arranged by size:
1686 865 35
DraIII CAC_nnn'GTG
Cuts at: 0 117 1061 2586
Size: 117 944 1525
Fragments arranged by size:
1525 944 117
EaeI y'GGCC_r
Cuts at: 0 847 2586
Size: 847 1739
EarI CTCTTCn'nnn_
Cuts at: 0 940 1402 2221 2227 2242 2586
Size: 940 462 819 6 15 344
Fragments arranged by size:
940 819 462 344 15 6
EcoICRI GAG'CTC
Cuts at: 0 1339 2586
Size: 1339 1247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1121 1584 2386 2575 2586
Size: 931 190 463 802 189 11
Fragments arranged by size:
931 802 463 190 189 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1876 2586
Size: 1876 710
EcoO109I rG'GnC_Cy
Cuts at: 0 794 899 1287 1491 1548 2586
Size: 794 105 388 204 57 1038
Fragments arranged by size:
1038 794 388 204 105 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 2586
Size: 198 2388
HaeII r_GCGC'y
Cuts at: 0 359 2586
Size: 359 2227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1094 1126 2586
Size: 1094 32 1460
Fragments arranged by size:
1460 1094 32
KpnI G_GTAC'C
Cuts at: 0 1087 2586
Size: 1087 1499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1763 2586
Size: 1763 823
MslI CAynn'nnrTG
Cuts at: 0 337 709 1039 2011 2586
Size: 337 372 330 972 575
Fragments arranged by size:
972 575 372 337 330
MspA1I CmG'CkG
Cuts at: 0 857 948 1095 1497 1714 2586
Size: 857 91 147 402 217 872
Fragments arranged by size:
872 857 402 217 147 91
NaeI GCC'GGC
Cuts at: 0 398 2586
Size: 398 2188
NgoMIV G'CCGG_C
Cuts at: 0 396 2586
Size: 396 2190
NspI r_CATG'y
Cuts at: 0 1029 2586
Size: 1029 1557
PflMI CCAn_nnn'nTGG
Cuts at: 0 1269 1290 2586
Size: 1269 21 1296
Fragments arranged by size:
1296 1269 21
PfoI T'CCnGG_A
Cuts at: 0 1400 1928 2586
Size: 1400 528 658
Fragments arranged by size:
1400 658 528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 2542 2574 2586
Size: 718 32 1792 32 12
Fragments arranged by size:
1792 718 32 32 12
PpuMI rG'GwC_Cy
Cuts at: 0 794 899 1548 2586
Size: 794 105 649 1038
Fragments arranged by size:
1038 794 649 105
PspXI vC'TCGA_Gb
Cuts at: 0 1360 2586
Size: 1360 1226
PstI C_TGCA'G
Cuts at: 0 347 1175 2586
Size: 347 828 1411
Fragments arranged by size:
1411 828 347
PvuII CAG'CTG
Cuts at: 0 948 2586
Size: 948 1638
SacI G_AGCT'C
Cuts at: 0 1341 2586
Size: 1341 1245
SacII CC_GC'GG
Cuts at: 0 1096 2586
Size: 1096 1490
SanDI GG'GwC_CC
Cuts at: 0 1548 2586
Size: 1548 1038
SapI GCTCTTCn'nnn_
Cuts at: 0 1402 2586
Size: 1402 1184
SexAI A'CCwGG_T
Cuts at: 0 113 2586
Size: 113 2473
SfcI C'TryA_G
Cuts at: 0 343 1171 2586
Size: 343 828 1415
Fragments arranged by size:
1415 828 343
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1498 2586
Size: 1498 1088
SmaI CCC'GGG
Cuts at: 0 199 905 1750 2586
Size: 199 706 845 836
Fragments arranged by size:
845 836 706 199
SmlI C'TyrA_G
Cuts at: 0 1360 2182 2586
Size: 1360 822 404
Fragments arranged by size:
1360 822 404
StyI C'CwwG_G
Cuts at: 0 610 2031 2586
Size: 610 1421 555
Fragments arranged by size:
1421 610 555
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 90 1053 1276 1618 2044 2464 2586
Size: 90 963 223 342 426 420 122
Fragments arranged by size:
963 426 420 342 223 122 90
TatI w'GTAC_w
Cuts at: 0 185 1022 2586
Size: 185 837 1564
Fragments arranged by size:
1564 837 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2076 2108 2586
Size: 2076 32 478
Fragments arranged by size:
2076 478 32
Tth111I GACn'n_nGTC
Cuts at: 0 688 2586
Size: 688 1898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1291 1926 2013 2016 2427 2586
Size: 1291 635 87 3 411 159
Fragments arranged by size:
1291 635 411 159 87 3
XhoI C'TCGA_G
Cuts at: 0 1360 2586
Size: 1360 1226
XmaI C'CCGG_G
Cuts at: 0 197 903 1748 2586
Size: 197 706 845 838
Fragments arranged by size:
845 838 706 197
XmnI GAAnn'nnTTC
Cuts at: 0 573 2586
Size: 573 2013
ZraI GAC'GTC
Cuts at: 0 530 2541 2586
Size: 530 2011 45
Fragments arranged by size:
2011 530 45
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AvaI BanI BanII
BbsI BcgI BciVI BglI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI
MslI MspA1I NaeI NgoMIV NspI PflMI PfoI PpiI
PpuMI PspXI PstI PvuII SacI SacII SanDI SapI
SexAI SfcI SfiI SmaI SmlI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglII
BmrI BmtI BpuEI BsiEI BsiWI BspEI BsrDI BssHII
BssSI BstBI BstYI BstZ17I ClaI DraI DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PshAI PsiI PspOMI PsrI PvuI RsrII SalI
SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI