Restriction map for
pF1KB1326
(Linear) (Six-base) MAPSORT of: KIBB1326.seq Check: 6361 from: 1 to: 2826
KIBB1326.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1431 2388 2652 2826
Size: 1431 957 264 174
Fragments arranged by size:
1431 957 264 174
AatII G_ACGT'C
Cuts at: 0 272 2826
Size: 272 2554
Acc65I G'GTAC_C
Cuts at: 0 1982 2826
Size: 1982 844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 750 919 1216 1696 2826
Size: 322 428 169 297 480 1130
Fragments arranged by size:
1130 480 428 322 297 169
AfeI AGC'GCT
Cuts at: 0 840 2826
Size: 840 1986
AflIII A'CryG_T
Cuts at: 0 1235 2826
Size: 1235 1591
AleI CACnn'nnGTG
Cuts at: 0 328 1585 2656 2826
Size: 328 1257 1071 170
Fragments arranged by size:
1257 1071 328 170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 97 1570 2826
Size: 97 1473 1256
Fragments arranged by size:
1473 1256 97
AlwNI CAG_nnn'CTG
Cuts at: 0 500 1398 2716 2826
Size: 500 898 1318 110
Fragments arranged by size:
1318 898 500 110
ApaI G_GGCC'C
Cuts at: 0 27 163 1005 1185 2826
Size: 27 136 842 180 1641
Fragments arranged by size:
1641 842 180 136 27
ApaLI G'TGCA_C
Cuts at: 0 321 2728 2826
Size: 321 2407 98
Fragments arranged by size:
2407 321 98
AvaI C'yCGr_G
Cuts at: 0 19 108 133 156 377 576 694 1015
Size: 19 89 25 23 221 199 118 321
Cuts at: 1015 1129 1321 1534 1882 2469 2691 2786 2826
Size: 114 192 213 348 587 222 95 40
Fragments arranged by size:
587 348 321 222 221 213 199 192
118 114 95 89 40 25 23 19
AvrII C'CTAG_G
Cuts at: 0 2821 2826
Size: 2821 5
BamHI G'GATC_C
Cuts at: 0 314 2826
Size: 314 2512
BanI G'GyrC_C
Cuts at: 0 333 429 524 826 970 1905 1963 1982
Size: 333 96 95 302 144 935 58 19
Cuts at: 1982 2826
Size: 844
Fragments arranged by size:
935 844 333 302 144 96 95 58
19
BanII G_rGCy'C
Cuts at: 0 20 27 91 156 163 386 1005 1173
Size: 20 7 64 65 7 223 619 168
Cuts at: 1173 1185 1889 2207 2470 2491 2678 2826
Size: 12 704 318 263 21 187 148
Fragments arranged by size:
704 619 318 263 223 187 168 148
65 64 21 20 12 7 7
BbeI G_GCGC'C
Cuts at: 0 830 1967 2826
Size: 830 1137 859
Fragments arranged by size:
1137 859 830
BbsI GAAGACnn'nnnn_
Cuts at: 0 610 1845 2826
Size: 610 1235 981
Fragments arranged by size:
1235 981 610
BbvCI CC'TCA_GC
Cuts at: 0 2737 2826
Size: 2737 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1976 2010 2229 2263 2826
Size: 1976 34 219 34 563
Fragments arranged by size:
1976 563 219 34 34
BclI T'GATC_A
Cuts at: 0 2702 2826
Size: 2702 124
BglI GCCn_nnn'nGGC
Cuts at: 0 1802 2167 2826
Size: 1802 365 659
Fragments arranged by size:
1802 659 365
BglII A'GATC_T
Cuts at: 0 902 1199 2826
Size: 902 297 1627
Fragments arranged by size:
1627 902 297
BlpI GC'TnA_GC
Cuts at: 0 2260 2826
Size: 2260 566
Bme1580I G_kGCm'C
Cuts at: 0 27 163 325 485 527 1005 1185 1910
Size: 27 136 162 160 42 478 180 725
Cuts at: 1910 2032 2699 2732 2826
Size: 122 667 33 94
Fragments arranged by size:
725 667 478 180 162 160 136 122
94 42 33 27
BmgBI CAC'GTC
Cuts at: 0 608 624 1365 1494 2826
Size: 608 16 741 129 1332
Fragments arranged by size:
1332 741 608 129 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 2420 2826
Size: 2420 406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1831 2656 2826
Size: 1831 825 170
Fragments arranged by size:
1831 825 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1006 1054 1303 1990 2560 2826
Size: 445 561 48 249 687 570 266
Fragments arranged by size:
687 570 561 445 266 249 48
Bpu10I CC'TnA_GC
Cuts at: 0 90 2737 2826
Size: 90 2647 89
Fragments arranged by size:
2647 90 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1783 2629 2826
Size: 1201 582 846 197
Fragments arranged by size:
1201 846 582 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 1802 2826
Size: 1802 1024
BsaAI yAC'GTr
Cuts at: 0 309 1236 1464 2337 2826
Size: 309 927 228 873 489
Fragments arranged by size:
927 873 489 309 228
BsaHI Gr'CG_yC
Cuts at: 0 146 269 711 827 1073 1964 2123 2561
Size: 146 123 442 116 246 891 159 438
Cuts at: 2561 2826
Size: 265
Fragments arranged by size:
891 442 438 265 246 159 146 123
116
BsaWI w'CCGG_w
Cuts at: 0 188 876 1826 2599 2606 2826
Size: 188 688 950 773 7 220
Fragments arranged by size:
950 773 688 220 188 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 422 452 1591 1621 1882 1912
Size: 214 30 178 30 1139 30 261 30
Cuts at: 1912 1966 1996 2077 2107 2826
Size: 54 30 81 30 719
Fragments arranged by size:
1139 719 261 214 178 81 54 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 606 991 1129 1552 1599 1987 2305 2632
Size: 606 385 138 423 47 388 318 327
Cuts at: 2632 2775 2826
Size: 143 51
Fragments arranged by size:
606 423 388 385 327 318 143 138
51 47
BseYI C'CCAG_C
Cuts at: 0 36 84 337 1081 1093 1952 2060 2458
Size: 36 48 253 744 12 859 108 398
Cuts at: 2458 2462 2826
Size: 4 364
Fragments arranged by size:
859 744 398 364 253 108 48 36
12 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1717 2198 2239 2302 2362 2680 2826
Size: 445 1272 481 41 63 60 318 146
Fragments arranged by size:
1272 481 445 318 146 63 60 41
BsiEI CG_ry'CG
Cuts at: 0 14 1305 2826
Size: 14 1291 1521
Fragments arranged by size:
1521 1291 14
BsiHKAI G_wGCw'C
Cuts at: 0 325 1264 1721 2132 2732 2826
Size: 325 939 457 411 600 94
Fragments arranged by size:
939 600 457 411 325 94
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1500 2826
Size: 1500 1326
Bsp1286I G_dGCh'C
Cuts at: 0 20 27 91 156 163 325 386 485
Size: 20 7 64 65 7 162 61 99
Cuts at: 485 527 1005 1173 1185 1264 1721 1889 1910
Size: 42 478 168 12 79 457 168 21
Cuts at: 1910 2032 2132 2207 2470 2491 2678 2699 2732
Size: 122 100 75 263 21 187 21 33
Cuts at: 2732 2826
Size: 94
Fragments arranged by size:
478 457 263 187 168 168 162 122
100 99 94 79 75 65 64 61
42 33 21 21 21 20 12 7
7
BspEI T'CCGG_A
Cuts at: 0 188 876 1826 2599 2606 2826
Size: 188 688 950 773 7 220
Fragments arranged by size:
950 773 688 220 188 7
BspHI T'CATG_A
Cuts at: 0 288 2826
Size: 288 2538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 578 1431 1736 1820 2388 2652 2826
Size: 578 853 305 84 568 264 174
Fragments arranged by size:
853 578 568 305 264 174 84
BsrBI CCG'CTC
Cuts at: 0 138 1271 1659 2406 2826
Size: 138 1133 388 747 420
Fragments arranged by size:
1133 747 420 388 138
BsrFI r'CCGG_y
Cuts at: 0 253 432 631 967 1043 1358 1406 1697
Size: 253 179 199 336 76 315 48 291
Cuts at: 1697 2826
Size: 1129
Fragments arranged by size:
1129 336 315 291 253 199 179 76
48
BsrGI T'GTAC_A
Cuts at: 0 1664 2567 2826
Size: 1664 903 259
Fragments arranged by size:
1664 903 259
BssHII G'CGCG_C
Cuts at: 0 101 103 583 2826
Size: 101 2 480 2243
Fragments arranged by size:
2243 480 101 2
BssSI C'ACGA_G
Cuts at: 0 324 2164 2826
Size: 324 1840 662
Fragments arranged by size:
1840 662 324
BstAPI GCAn_nnn'nTGC
Cuts at: 0 500 2826
Size: 500 2326
BstXI CCAn_nnnn'nTGG
Cuts at: 0 520 1157 2154 2170 2826
Size: 520 637 997 16 656
Fragments arranged by size:
997 656 637 520 16
BstYI r'GATC_y
Cuts at: 0 314 902 1199 2387 2826
Size: 314 588 297 1188 439
Fragments arranged by size:
1188 588 439 314 297
Bsu36I CC'TnA_GG
Cuts at: 0 2723 2826
Size: 2723 103
BtgI C'CryG_G
Cuts at: 0 680 800 845 1177 1583 2826
Size: 680 120 45 332 406 1243
Fragments arranged by size:
1243 680 406 332 120 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1363 1952 2826
Size: 1363 589 874
Fragments arranged by size:
1363 874 589
BtsI GCAGTG_nn'
Cuts at: 0 363 441 501 994 1706 2181 2826
Size: 363 78 60 493 712 475 645
Fragments arranged by size:
712 645 493 475 363 78 60
EaeI y'GGCC_r
Cuts at: 0 11 251 629 965 1026 1302 2012 2826
Size: 11 240 378 336 61 276 710 814
Fragments arranged by size:
814 710 378 336 276 240 61 11
EagI C'GGCC_G
Cuts at: 0 11 1302 2826
Size: 11 1291 1524
Fragments arranged by size:
1524 1291 11
EarI CTCTTCn'nnn_
Cuts at: 0 1252 1883 2282 2826
Size: 1252 631 399 544
Fragments arranged by size:
1252 631 544 399
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 931 2806 2826
Size: 931 1875 20
Fragments arranged by size:
1875 931 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 445 750 919 1006 1054 1216 1303
Size: 322 123 305 169 87 48 162 87
Cuts at: 1303 1696 1990 2560 2826
Size: 393 294 570 266
Fragments arranged by size:
570 393 322 305 294 266 169 162
123 87 87 48
EcoO109I rG'GnC_Cy
Cuts at: 0 23 130 160 420 1020 1191 1287 1873
Size: 23 107 30 260 600 171 96 586
Cuts at: 1873 2085 2688 2826
Size: 212 603 138
Fragments arranged by size:
603 600 586 260 212 171 138 107
96 30 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 231 2826
Size: 231 2595
HaeII r_GCGC'y
Cuts at: 0 439 688 830 842 1967 2826
Size: 439 249 142 12 1125 859
Fragments arranged by size:
1125 859 439 249 142 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2077 2109 2826
Size: 2077 32 717
Fragments arranged by size:
2077 717 32
KasI G'GCGC_C
Cuts at: 0 826 1963 2826
Size: 826 1137 863
Fragments arranged by size:
1137 863 826
KpnI G_GTAC'C
Cuts at: 0 1986 2826
Size: 1986 840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1610 2516 2826
Size: 257 1353 906 310
Fragments arranged by size:
1353 906 310 257
MscI TGG'CCA
Cuts at: 0 1028 2014 2826
Size: 1028 986 812
Fragments arranged by size:
1028 986 812
MslI CAynn'nnrTG
Cuts at: 0 328 518 1585 1687 2656 2826
Size: 328 190 1067 102 969 170
Fragments arranged by size:
1067 969 328 190 170 102
MspA1I CmG'CkG
Cuts at: 0 166 682 802 1179 1296 1335 1371 1757
Size: 166 516 120 377 117 39 36 386
Cuts at: 1757 2060 2078 2141 2462 2713 2826
Size: 303 18 63 321 251 113
Fragments arranged by size:
516 386 377 321 303 251 166 120
117 113 63 39 36 18
NaeI GCC'GGC
Cuts at: 0 255 633 1045 1360 1699 2826
Size: 255 378 412 315 339 1127
Fragments arranged by size:
1127 412 378 339 315 255
NarI GG'CG_CC
Cuts at: 0 827 1964 2826
Size: 827 1137 862
Fragments arranged by size:
1137 862 827
NgoMIV G'CCGG_C
Cuts at: 0 253 631 1043 1358 1697 2826
Size: 253 378 412 315 339 1129
Fragments arranged by size:
1129 412 378 339 315 253
NspI r_CATG'y
Cuts at: 0 1671 2736 2826
Size: 1671 1065 90
Fragments arranged by size:
1671 1065 90
PasI CC'CwG_GG
Cuts at: 0 2025 2616 2678 2826
Size: 2025 591 62 148
Fragments arranged by size:
2025 591 148 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 1338 2826
Size: 1338 1488
PfoI T'CCnGG_A
Cuts at: 0 422 883 2826
Size: 422 461 1943
Fragments arranged by size:
1943 461 422
PmlI CAC'GTG
Cuts at: 0 309 2826
Size: 309 2517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 2074 2106 2826
Size: 559 32 1483 32 720
Fragments arranged by size:
1483 720 559 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 130 420 1020 1191 1287 1873 2085 2826
Size: 130 290 600 171 96 586 212 741
Fragments arranged by size:
741 600 586 290 212 171 130 96
PshAI GACnn'nnGTC
Cuts at: 0 1757 2045 2560 2826
Size: 1757 288 515 266
Fragments arranged by size:
1757 515 288 266
PspOMI G'GGCC_C
Cuts at: 0 23 159 1001 1181 2826
Size: 23 136 842 180 1645
Fragments arranged by size:
1645 842 180 136 23
PstI C_TGCA'G
Cuts at: 0 76 496 601 1822 2354 2399 2826
Size: 76 420 105 1221 532 45 427
Fragments arranged by size:
1221 532 427 420 105 76 45
PvuII CAG'CTG
Cuts at: 0 1335 1757 2462 2713 2826
Size: 1335 422 705 251 113
Fragments arranged by size:
1335 705 422 251 113
SacII CC_GC'GG
Cuts at: 0 683 803 1180 2826
Size: 683 120 377 1646
Fragments arranged by size:
1646 683 377 120
SanDI GG'GwC_CC
Cuts at: 0 130 2826
Size: 130 2696
SapI GCTCTTCn'nnn_
Cuts at: 0 1252 2826
Size: 1252 1574
SexAI A'CCwGG_T
Cuts at: 0 2503 2826
Size: 2503 323
SfcI C'TryA_G
Cuts at: 0 72 356 492 597 1411 1818 2073 2350
Size: 72 284 136 105 814 407 255 277
Cuts at: 2350 2395 2826
Size: 45 431
Fragments arranged by size:
814 431 407 284 277 255 136 105
72 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1802 2826
Size: 1802 1024
SfoI GGC'GCC
Cuts at: 0 828 1965 2826
Size: 828 1137 861
Fragments arranged by size:
1137 861 828
SgrAI Cr'CCGG_yG
Cuts at: 0 432 2826
Size: 432 2394
SmaI CCC'GGG
Cuts at: 0 21 110 158 379 1131 1884 2693 2788
Size: 21 89 48 221 752 753 809 95
Cuts at: 2788 2826
Size: 38
Fragments arranged by size:
809 753 752 221 95 89 48 38
21
SmlI C'TyrA_G
Cuts at: 0 1216 1798 2644 2826
Size: 1216 582 846 182
Fragments arranged by size:
1216 846 582 182
SrfI GCCC'GGGC
Cuts at: 0 2788 2826
Size: 2788 38
StuI AGG'CCT
Cuts at: 0 1066 1156 1796 2438 2826
Size: 1066 90 640 642 388
Fragments arranged by size:
1066 642 640 388 90
StyI C'CwwG_G
Cuts at: 0 1614 2043 2821 2826
Size: 1614 429 778 5
Fragments arranged by size:
1614 778 429 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 351 485 556 1944 2826
Size: 351 134 71 1388 882
Fragments arranged by size:
1388 882 351 134 71
TatI w'GTAC_w
Cuts at: 0 1541 1664 2567 2826
Size: 1541 123 903 259
Fragments arranged by size:
1541 903 259 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 422 454 1591 1623 1882 1914
Size: 214 32 176 32 1137 32 259 32
Cuts at: 1914 2826
Size: 912
Fragments arranged by size:
1137 912 259 214 176 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1581 2022 2584 2826
Size: 1581 441 562 242
Fragments arranged by size:
1581 562 441 242
XmaI C'CCGG_G
Cuts at: 0 19 108 156 377 1129 1882 2691 2786
Size: 19 89 48 221 752 753 809 95
Cuts at: 2786 2826
Size: 40
Fragments arranged by size:
809 753 752 221 95 89 48 40
19
ZraI GAC'GTC
Cuts at: 0 270 2826
Size: 270 2556
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I
FalI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacII
SanDI SapI SexAI SfcI SfiI SfoI SgrAI SmaI
SmlI SrfI StuI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AloI ApoI AscI
AseI AsiSI BaeI BciVI BfrBI BmtI BsaBI BsiWI
BsmI BsrDI BstBI BstEII BstZ17I ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PsiI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SgfI SnaBI SpeI SphI SspI SwaI Tth111I XbaI
XhoI XmnI