Restriction map for
pF1KB1434
(Linear) (Six-base) MAPSORT of: KIBB1434.seq Check: 287 from: 1 to: 6306
KIBB1434.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1712 3245 6306
Size: 1712 1533 3061
Fragments arranged by size:
3061 1712 1533
AatII G_ACGT'C
Cuts at: 0 1411 6306
Size: 1411 4895
AccI GT'mk_AC
Cuts at: 0 2069 6306
Size: 2069 4237
Acc65I G'GTAC_C
Cuts at: 0 745 6306
Size: 745 5561
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 375 772 1089 1750 3040 4140 4781
Size: 253 122 397 317 661 1290 1100 641
Cuts at: 4781 5318 6306
Size: 537 988
Fragments arranged by size:
1290 1100 988 661 641 537 397 317
253 122
AfeI AGC'GCT
Cuts at: 0 3066 3976 6306
Size: 3066 910 2330
Fragments arranged by size:
3066 2330 910
AflIII A'CryG_T
Cuts at: 0 3134 5228 6306
Size: 3134 2094 1078
Fragments arranged by size:
3134 2094 1078
AhdI GACnn_n'nnGTC
Cuts at: 0 734 5367 6306
Size: 734 4633 939
Fragments arranged by size:
4633 939 734
AleI CACnn'nnGTG
Cuts at: 0 5227 6306
Size: 5227 1079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4486 4691 5408 5905 6306
Size: 4486 205 717 497 401
Fragments arranged by size:
4486 717 497 401 205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3724 3756 6306
Size: 3724 32 2550
Fragments arranged by size:
3724 2550 32
AlwNI CAG_nnn'CTG
Cuts at: 0 398 1594 2728 2910 3048 5046 6306
Size: 398 1196 1134 182 138 1998 1260
Fragments arranged by size:
1998 1260 1196 1134 398 182 138
ApaI G_GGCC'C
Cuts at: 0 2266 4043 6306
Size: 2266 1777 2263
Fragments arranged by size:
2266 2263 1777
ApaLI G'TGCA_C
Cuts at: 0 1498 6306
Size: 1498 4808
ApoI r'AATT_y
Cuts at: 0 1930 3787 4225 6213 6306
Size: 1930 1857 438 1988 93
Fragments arranged by size:
1988 1930 1857 438 93
AseI AT'TA_AT
Cuts at: 0 2129 6306
Size: 2129 4177
AvaI C'yCGr_G
Cuts at: 0 17 2626 4737 5711 6306
Size: 17 2609 2111 974 595
Fragments arranged by size:
2609 2111 974 595 17
BamHI G'GATC_C
Cuts at: 0 435 1268 6306
Size: 435 833 5038
Fragments arranged by size:
5038 833 435
BanI G'GyrC_C
Cuts at: 0 367 745 1724 3213 3481 4836 5468 6306
Size: 367 378 979 1489 268 1355 632 838
Fragments arranged by size:
1489 1355 979 838 632 378 367 268
BanII G_rGCy'C
Cuts at: 0 1241 1288 1907 2266 2434 2591 4043 4435
Size: 1241 47 619 359 168 157 1452 392
Cuts at: 4435 4817 6306
Size: 382 1489
Fragments arranged by size:
1489 1452 1241 619 392 382 359 168
157 47
BbeI G_GCGC'C
Cuts at: 0 4840 6306
Size: 4840 1466
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 3028 4211 5080 6306
Size: 310 2718 1183 869 1226
Fragments arranged by size:
2718 1226 1183 869 310
BbvCI CC'TCA_GC
Cuts at: 0 3557 6247 6306
Size: 3557 2690 59
Fragments arranged by size:
3557 2690 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 859 893 1342 1376 4786 4820 6306
Size: 859 34 449 34 3410 34 1486
Fragments arranged by size:
3410 1486 859 449 34 34 34
BclI T'GATC_A
Cuts at: 0 6005 6306
Size: 6005 301
BglI GCCn_nnn'nGGC
Cuts at: 0 2275 6306
Size: 2275 4031
BglII A'GATC_T
Cuts at: 0 3953 4130 4585 5522 6306
Size: 3953 177 455 937 784
Fragments arranged by size:
3953 937 784 455 177
BlpI GC'TnA_GC
Cuts at: 0 1282 2360 2576 3373 4816 6306
Size: 1282 1078 216 797 1443 1490
Fragments arranged by size:
1490 1443 1282 1078 797 216
Bme1580I G_kGCm'C
Cuts at: 0 372 1502 2266 3216 4043 6306
Size: 372 1130 764 950 827 2263
Fragments arranged by size:
2263 1130 950 827 764 372
BmrI ACTGGGnnnn_n'
Cuts at: 0 812 1073 1540 1747 3276 6306
Size: 812 261 467 207 1529 3030
Fragments arranged by size:
3030 1529 812 467 261 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 388 3975 4667 6306
Size: 388 3587 692 1639
Fragments arranged by size:
3587 1639 692 388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 848 900 1225 1231 2245 2518 2716
Size: 54 794 52 325 6 1014 273 198
Cuts at: 2716 2911 3024 3178 3945 4602 4662 4793 4857
Size: 195 113 154 767 657 60 131 64
Cuts at: 4857 6306
Size: 1449
Fragments arranged by size:
1449 1014 794 767 657 325 273 198
195 154 131 113 64 60 54 52
6
Bpu10I CC'TnA_GC
Cuts at: 0 2433 3557 6247 6306
Size: 2433 1124 2690 59
Fragments arranged by size:
2690 2433 1124 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1899 4166 6306
Size: 1899 2267 2140
Fragments arranged by size:
2267 2140 1899
BsaI GGTCTCn'nnnn_
Cuts at: 0 129 6306
Size: 129 6177
BsaAI yAC'GTr
Cuts at: 0 3137 4026 6306
Size: 3137 889 2280
Fragments arranged by size:
3137 2280 889
BsaBI GATnn'nnATC
Cuts at: 0 565 2729 6306
Size: 565 2164 3577
Fragments arranged by size:
3577 2164 565
BsaHI Gr'CG_yC
Cuts at: 0 1408 3107 4799 4837 4943 6306
Size: 1408 1699 1692 38 106 1363
Fragments arranged by size:
1699 1692 1408 1363 106 38
BsaWI w'CCGG_w
Cuts at: 0 1265 1996 3116 3656 4938 6306
Size: 1265 731 1120 540 1282 1368
Fragments arranged by size:
1368 1282 1265 1120 731 540
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 688 718 3719 3749 4417 4447 5692 5722
Size: 688 30 3001 30 668 30 1245 30
Cuts at: 5722 6306
Size: 584
Fragments arranged by size:
3001 1245 688 668 584 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 629 1036 2668 2896 2899 5164 6306
Size: 601 28 407 1632 228 3 2265 1142
Fragments arranged by size:
2265 1632 1142 601 407 228 28 3
BseYI C'CCAG_C
Cuts at: 0 715 1977 2217 2506 3284 3644 3698 4436
Size: 715 1262 240 289 778 360 54 738
Cuts at: 4436 4832 5039 5175 5329 5721 5966 6306
Size: 396 207 136 154 392 245 340
Fragments arranged by size:
1262 778 738 715 396 392 360 340
289 245 240 207 154 136 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1382 2233 2309 3158 3928 4426 4687
Size: 151 1231 851 76 849 770 498 261
Cuts at: 4687 4993 5329 5514 5688 6306
Size: 306 336 185 174 618
Fragments arranged by size:
1231 851 849 770 618 498 336 306
261 185 174 151 76
BsiEI CG_ry'CG
Cuts at: 0 6101 6306
Size: 6101 205
BsiHKAI G_wGCw'C
Cuts at: 0 1241 1502 2171 2330 2591 4817 6306
Size: 1241 261 669 159 261 2226 1489
Fragments arranged by size:
2226 1489 1241 669 261 261 159
BsiWI C'GTAC_G
Cuts at: 0 3137 6306
Size: 3137 3169
BsmI GAATG_Cn'
Cuts at: 0 1317 1629 6306
Size: 1317 312 4677
Fragments arranged by size:
4677 1317 312
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3676 4510 4726 5549 6108 6306
Size: 3676 834 216 823 559 198
Fragments arranged by size:
3676 834 823 559 216 198
Bsp1286I G_dGCh'C
Cuts at: 0 372 1241 1288 1502 1907 2171 2266 2330
Size: 372 869 47 214 405 264 95 64
Cuts at: 2330 2434 2591 3216 4043 4435 4817 6306
Size: 104 157 625 827 392 382 1489
Fragments arranged by size:
1489 869 827 625 405 392 382 372
264 214 157 104 95 64 47
BspEI T'CCGG_A
Cuts at: 0 3656 4938 6306
Size: 3656 1282 1368
Fragments arranged by size:
3656 1368 1282
BspHI T'CATG_A
Cuts at: 0 2189 4217 6221 6306
Size: 2189 2028 2004 85
Fragments arranged by size:
2189 2028 2004 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 1712 2618 3245 5333 6306
Size: 465 1247 906 627 2088 973
Fragments arranged by size:
2088 1247 973 906 627 465
BsrBI CCG'CTC
Cuts at: 0 119 704 2895 3008 5474 6306
Size: 119 585 2191 113 2466 832
Fragments arranged by size:
2466 2191 832 585 119 113
BsrDI GCAATG_nn'
Cuts at: 0 1605 1865 2109 3599 4119 6306
Size: 1605 260 244 1490 520 2187
Fragments arranged by size:
2187 1605 1490 520 260 244
BsrFI r'CCGG_y
Cuts at: 0 1790 2690 4019 5564 6306
Size: 1790 900 1329 1545 742
Fragments arranged by size:
1790 1545 1329 900 742
BssSI C'ACGA_G
Cuts at: 0 1224 6306
Size: 1224 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 0 235 480 5574 6306
Size: 235 245 5094 732
Fragments arranged by size:
5094 732 245 235
BstBI TT'CG_AA
Cuts at: 0 678 6306
Size: 678 5628
BstEII G'GTnAC_C
Cuts at: 0 2565 4779 6306
Size: 2565 2214 1527
Fragments arranged by size:
2565 2214 1527
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1065 2453 3417 5352 6306
Size: 1065 1388 964 1935 954
Fragments arranged by size:
1935 1388 1065 964 954
BstYI r'GATC_y
Cuts at: 0 197 435 832 1193 1268 1916 2724 3525
Size: 197 238 397 361 75 648 808 801
Cuts at: 3525 3953 4130 4585 5522 6306
Size: 428 177 455 937 784
Fragments arranged by size:
937 808 801 784 648 455 428 397
361 238 197 177 75
BstZ17I GTA'TAC
Cuts at: 0 2070 6306
Size: 2070 4236
Bsu36I CC'TnA_GG
Cuts at: 0 4981 5339 6306
Size: 4981 358 967
Fragments arranged by size:
4981 967 358
BtgI C'CryG_G
Cuts at: 0 192 2097 3302 4841 6306
Size: 192 1905 1205 1539 1465
Fragments arranged by size:
1905 1539 1465 1205 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1686 2130 5445 6306
Size: 1686 444 3315 861
Fragments arranged by size:
3315 1686 861 444
BtsI GCAGTG_nn'
Cuts at: 0 236 774 2129 2292 2518 2806 4139 4621
Size: 236 538 1355 163 226 288 1333 482
Cuts at: 4621 5663 6306
Size: 1042 643
Fragments arranged by size:
1355 1333 1042 643 538 482 288 236
226 163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4128 4163 6306
Size: 4128 35 2143
Fragments arranged by size:
4128 2143 35
DraIII CAC_nnn'GTG
Cuts at: 0 1506 2175 3181 6306
Size: 1506 669 1006 3125
Fragments arranged by size:
3125 1506 1006 669
DrdI GACnn_nn'nnGTC
Cuts at: 0 1531 4321 6100 6306
Size: 1531 2790 1779 206
Fragments arranged by size:
2790 1779 1531 206
EaeI y'GGCC_r
Cuts at: 0 1775 2138 2150 2443 3299 3305 4031 4487
Size: 1775 363 12 293 856 6 726 456
Cuts at: 4487 4574 5411 5514 5660 5828 6150 6306
Size: 87 837 103 146 168 322 156
Fragments arranged by size:
1775 856 837 726 456 363 322 293
168 156 146 103 87 12 6
EarI CTCTTCn'nnn_
Cuts at: 0 596 656 748 1660 2038 2386 4957 5090
Size: 596 60 92 912 378 348 2571 133
Cuts at: 5090 5605 6306
Size: 515 701
Fragments arranged by size:
2571 912 701 596 515 378 348 133
92 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 41 47 53 2869 6306
Size: 41 6 6 2816 3437
Fragments arranged by size:
3437 2816 41 6 6
EcoICRI GAG'CTC
Cuts at: 0 1239 2589 4815 6306
Size: 1239 1350 2226 1491
Fragments arranged by size:
2226 1491 1350 1239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 253 375 772 848 900 1089 1225
Size: 54 199 122 397 76 52 189 136
Cuts at: 1225 1231 1750 2245 2518 2716 2911 3024 3040
Size: 6 519 495 273 198 195 113 16
Cuts at: 3040 3178 3945 4140 4602 4662 4781 4793 4857
Size: 138 767 195 462 60 119 12 64
Cuts at: 4857 5318 6306
Size: 461 988
Fragments arranged by size:
988 767 519 495 462 461 397 273
199 198 195 195 189 138 136 122
119 113 76 64 60 54 52 16
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3196 4181 6306
Size: 3196 985 2125
Fragments arranged by size:
3196 2125 985
EcoO109I rG'GnC_Cy
Cuts at: 0 1167 2262 2263 3649 3761 4040 4977 5375
Size: 1167 1095 1 1386 112 279 937 398
Cuts at: 5375 6306
Size: 931
Fragments arranged by size:
1386 1167 1095 937 931 398 279 112
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 550 2508 2673 5628 6306
Size: 550 1958 165 2955 678
Fragments arranged by size:
2955 1958 678 550 165
FspI TGC'GCA
Cuts at: 0 3436 6306
Size: 3436 2870
HaeII r_GCGC'y
Cuts at: 0 77 3068 3384 3978 4840 6306
Size: 77 2991 316 594 862 1466
Fragments arranged by size:
2991 1466 862 594 316 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 688 720 752 1437 1469 4488 4520 5353
Size: 688 32 32 685 32 3019 32 833
Cuts at: 5353 5385 5692 5724 6306
Size: 32 307 32 582
Fragments arranged by size:
3019 833 688 685 582 307 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 0 1640 3850 6306
Size: 1640 2210 2456
Fragments arranged by size:
2456 2210 1640
HindIII A'AGCT_T
Cuts at: 0 163 6306
Size: 163 6143
KasI G'GCGC_C
Cuts at: 0 4836 6306
Size: 4836 1470
KpnI G_GTAC'C
Cuts at: 0 749 6306
Size: 749 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1811 2180 3434 4712 6104 6306
Size: 1811 369 1254 1278 1392 202
Fragments arranged by size:
1811 1392 1278 1254 369 202
MscI TGG'CCA
Cuts at: 0 1777 2140 2445 3301 3307 4489 4576 5413
Size: 1777 363 305 856 6 1182 87 837
Cuts at: 5413 5662 6306
Size: 249 644
Fragments arranged by size:
1777 1182 856 837 644 363 305 249
87 6
MslI CAynn'nnrTG
Cuts at: 0 1870 3775 4085 5227 6052 6118 6306
Size: 1870 1905 310 1142 825 66 188
Fragments arranged by size:
1905 1870 1142 825 310 188 66
MspA1I CmG'CkG
Cuts at: 0 1977 2217 2575 3372 3912 4160 4379 4440
Size: 1977 240 358 797 540 248 219 61
Cuts at: 4440 4527 4671 5196 5619 5721 6306
Size: 87 144 525 423 102 585
Fragments arranged by size:
1977 797 585 540 525 423 358 248
240 219 144 102 87 61
NaeI GCC'GGC
Cuts at: 0 5566 6306
Size: 5566 740
NarI GG'CG_CC
Cuts at: 0 4837 6306
Size: 4837 1469
NcoI C'CATG_G
Cuts at: 0 192 3302 6306
Size: 192 3110 3004
Fragments arranged by size:
3110 3004 192
NgoMIV G'CCGG_C
Cuts at: 0 5564 6306
Size: 5564 742
NruI TCG'CGA
Cuts at: 0 573 4124 6306
Size: 573 3551 2182
Fragments arranged by size:
3551 2182 573
NspI r_CATG'y
Cuts at: 0 177 487 810 4084 4386 4413 4881 5577
Size: 177 310 323 3274 302 27 468 696
Cuts at: 5577 5748 6306
Size: 171 558
Fragments arranged by size:
3274 696 558 468 323 310 302 177
171 27
PasI CC'CwG_GG
Cuts at: 0 4062 5671 6306
Size: 4062 1609 635
Fragments arranged by size:
4062 1609 635
PflMI CCAn_nnn'nTGG
Cuts at: 0 510 3209 5046 6306
Size: 510 2699 1837 1260
Fragments arranged by size:
2699 1837 1260 510
PfoI T'CCnGG_A
Cuts at: 0 656 3194 3521 5132 6306
Size: 656 2538 327 1611 1174
Fragments arranged by size:
2538 1611 1174 656 327
PmlI CAC'GTG
Cuts at: 0 4026 6306
Size: 4026 2280
PpuMI rG'GwC_Cy
Cuts at: 0 1167 3649 5375 6306
Size: 1167 2482 1726 931
Fragments arranged by size:
2482 1726 1167 931
PshAI GACnn'nnGTC
Cuts at: 0 3548 6306
Size: 3548 2758
PspOMI G'GGCC_C
Cuts at: 0 2262 4039 6306
Size: 2262 1777 2267
Fragments arranged by size:
2267 2262 1777
PstI C_TGCA'G
Cuts at: 0 1400 1460 1723 1916 3044 3242 3937 3974
Size: 1400 60 263 193 1128 198 695 37
Cuts at: 3974 5588 5861 6306
Size: 1614 273 445
Fragments arranged by size:
1614 1400 1128 695 445 273 263 198
193 60 37
PvuII CAG'CTG
Cuts at: 0 1977 2217 3372 4160 4440 4671 5619 5721
Size: 1977 240 1155 788 280 231 948 102
Cuts at: 5721 6306
Size: 585
Fragments arranged by size:
1977 1155 948 788 585 280 240 231
102
RsrII CG'GwC_CG
Cuts at: 0 2525 6306
Size: 2525 3781
SacI G_AGCT'C
Cuts at: 0 1241 2591 4817 6306
Size: 1241 1350 2226 1489
Fragments arranged by size:
2226 1489 1350 1241
SanDI GG'GwC_CC
Cuts at: 0 3649 5375 6306
Size: 3649 1726 931
Fragments arranged by size:
3649 1726 931
SapI GCTCTTCn'nnn_
Cuts at: 0 656 1660 2386 6306
Size: 656 1004 726 3920
Fragments arranged by size:
3920 1004 726 656
SbfI CC_TGCA'GG
Cuts at: 0 3242 6306
Size: 3242 3064
ScaI AGT'ACT
Cuts at: 0 895 2306 3931 6283 6306
Size: 895 1411 1625 2352 23
Fragments arranged by size:
2352 1625 1411 895 23
SexAI A'CCwGG_T
Cuts at: 0 1001 2171 6306
Size: 1001 1170 4135
Fragments arranged by size:
4135 1170 1001
SfcI C'TryA_G
Cuts at: 0 429 1396 1456 1719 1912 1921 2103 3040
Size: 429 967 60 263 193 9 182 937
Cuts at: 3040 3238 3933 3970 5584 5835 5857 6306
Size: 198 695 37 1614 251 22 449
Fragments arranged by size:
1614 967 937 695 449 429 263 251
198 193 182 60 37 22 9
SfoI GGC'GCC
Cuts at: 0 4838 6306
Size: 4838 1468
SmaI CCC'GGG
Cuts at: 0 19 4739 5713 6306
Size: 19 4720 974 593
Fragments arranged by size:
4720 974 593 19
SmlI C'TyrA_G
Cuts at: 0 1878 4181 6306
Size: 1878 2303 2125
Fragments arranged by size:
2303 2125 1878
SphI G_CATG'C
Cuts at: 0 810 4084 6306
Size: 810 3274 2222
Fragments arranged by size:
3274 2222 810
StuI AGG'CCT
Cuts at: 0 508 4859 6306
Size: 508 4351 1447
Fragments arranged by size:
4351 1447 508
StyI C'CwwG_G
Cuts at: 0 146 192 509 1856 2870 3302 4004 4347
Size: 146 46 317 1347 1014 432 702 343
Cuts at: 4347 6306
Size: 1959
Fragments arranged by size:
1959 1347 1014 702 432 343 317 146
46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2361 4360 4866 5663 6306
Size: 2361 1999 506 797 643
Fragments arranged by size:
2361 1999 797 643 506
TatI w'GTAC_w
Cuts at: 0 893 1154 2304 2699 3929 4592 6281 6306
Size: 893 261 1150 395 1230 663 1689 25
Fragments arranged by size:
1689 1230 1150 893 663 395 261 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1730 1762 2408 2440 3719 3751 6306
Size: 1730 32 646 32 1279 32 2555
Fragments arranged by size:
2555 1730 1279 646 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1512 2563 3048 6097 6306
Size: 1512 1051 485 3049 209
Fragments arranged by size:
3049 1512 1051 485 209
XbaI T'CTAG_A
Cuts at: 0 5088 6306
Size: 5088 1218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 329 1591 5547 5923 6306
Size: 329 1262 3956 376 383
Fragments arranged by size:
3956 1262 383 376 329
XmaI C'CCGG_G
Cuts at: 0 17 4737 5711 6306
Size: 17 4720 974 595
Fragments arranged by size:
4720 974 595 17
XmnI GAAnn'nnTTC
Cuts at: 0 674 684 983 2032 2380 3166 5611 6306
Size: 674 10 299 1049 348 786 2445 695
Fragments arranged by size:
2445 1049 786 695 674 348 299 10
ZraI GAC'GTC
Cuts at: 0 1409 6306
Size: 1409 4897
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII RsrII
SacI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AscI AsiSI AvrII BaeI BciVI
BfrBI BmgBI BmtI BsrGI BssHII ClaI DraI EagI
EcoRI EcoRV FseI FspAI HpaI MfeI MluI NdeI
NheI NotI NsiI PacI PciI PmeI PpiI PsiI
PspXI PsrI PvuI SacII SalI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XhoI