Restriction map for
pF1KSDB1480
(Linear) (Six-base) MAPSORT of: KIBB1480.seq Check: 2356 from: 1 to: 2958
KIBB1480.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1863 2958
Size: 1863 1095
AatII G_ACGT'C
Cuts at: 0 2831 2958
Size: 2831 127
AccI GT'mk_AC
Cuts at: 0 1070 2958
Size: 1070 1888
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1939 2958
Size: 911 1028 1019
Fragments arranged by size:
1028 1019 911
AflIII A'CryG_T
Cuts at: 0 188 2958
Size: 188 2770
AhdI GACnn_n'nnGTC
Cuts at: 0 2832 2958
Size: 2832 126
AleI CACnn'nnGTG
Cuts at: 0 484 2080 2958
Size: 484 1596 878
Fragments arranged by size:
1596 878 484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2719 2958
Size: 2719 239
AlwNI CAG_nnn'CTG
Cuts at: 0 88 2958
Size: 88 2870
AvaI C'yCGr_G
Cuts at: 0 665 1098 2553 2958
Size: 665 433 1455 405
Fragments arranged by size:
1455 665 433 405
BanI G'GyrC_C
Cuts at: 0 236 244 610 744 796 1108 1501 2679
Size: 236 8 366 134 52 312 393 1178
Cuts at: 2679 2958
Size: 279
Fragments arranged by size:
1178 393 366 312 279 236 134 52
8
BanII G_rGCy'C
Cuts at: 0 1255 1433 2300 2958
Size: 1255 178 867 658
Fragments arranged by size:
1255 867 658 178
BbeI G_GCGC'C
Cuts at: 0 240 248 614 1505 2683 2958
Size: 240 8 366 891 1178 275
Fragments arranged by size:
1178 891 366 275 240 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 567 1506 1650 2958
Size: 567 939 144 1308
Fragments arranged by size:
1308 939 567 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 660 694 1289 1323 2238 2272 2509 2543
Size: 660 34 595 34 915 34 237 34
Cuts at: 2543 2544 2578 2958
Size: 1 34 380
Fragments arranged by size:
915 660 595 380 237 34 34 34
34 34 1
BciVI GTATCCnnnnn_n'
Cuts at: 0 408 2920 2958
Size: 408 2512 38
Fragments arranged by size:
2512 408 38
BclI T'GATC_A
Cuts at: 0 2087 2958
Size: 2087 871
BfrBI ATG'CAT
Cuts at: 0 1326 2958
Size: 1326 1632
BglI GCCn_nnn'nGGC
Cuts at: 0 753 920 1157 2958
Size: 753 167 237 1801
Fragments arranged by size:
1801 753 237 167
BglII A'GATC_T
Cuts at: 0 1781 1940 2958
Size: 1781 159 1018
Fragments arranged by size:
1781 1018 159
BlpI GC'TnA_GC
Cuts at: 0 92 2958
Size: 92 2866
Bme1580I G_kGCm'C
Cuts at: 0 592 749 1530 2349 2958
Size: 592 157 781 819 609
Fragments arranged by size:
819 781 609 592 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 1200 2490 2958
Size: 1200 1290 468
Fragments arranged by size:
1290 1200 468
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 456 1081 2130 2958
Size: 456 625 1049 828
Fragments arranged by size:
1049 828 625 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 2074 2785 2958
Size: 1045 1029 711 173
Fragments arranged by size:
1045 1029 711 173
Bpu10I CC'TnA_GC
Cuts at: 0 2002 2958
Size: 2002 956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1948 2958
Size: 1948 1010
BsaI GGTCTCn'nnnn_
Cuts at: 0 319 1003 2958
Size: 319 684 1955
Fragments arranged by size:
1955 684 319
BsaAI yAC'GTr
Cuts at: 0 173 261 2958
Size: 173 88 2697
Fragments arranged by size:
2697 173 88
BsaBI GATnn'nnATC
Cuts at: 0 1786 2089 2365 2958
Size: 1786 303 276 593
Fragments arranged by size:
1786 593 303 276
BsaHI Gr'CG_yC
Cuts at: 0 237 245 611 1502 2680 2828 2958
Size: 237 8 366 891 1178 148 130
Fragments arranged by size:
1178 891 366 237 148 130 8
BsaWI w'CCGG_w
Cuts at: 0 1048 2399 2958
Size: 1048 1351 559
Fragments arranged by size:
1351 1048 559
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 2443 2473 2958
Size: 256 30 2157 30 485
Fragments arranged by size:
2157 485 256 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1629 2679 2958
Size: 1629 1050 279
Fragments arranged by size:
1629 1050 279
BseYI C'CCAG_C
Cuts at: 0 15 96 301 1370 2958
Size: 15 81 205 1069 1588
Fragments arranged by size:
1588 1069 205 81 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1098 1504 2548 2958
Size: 945 153 406 1044 410
Fragments arranged by size:
1044 945 410 406 153
BsiEI CG_ry'CG
Cuts at: 0 1646 2958
Size: 1646 1312
BsiHKAI G_wGCw'C
Cuts at: 0 649 1433 2077 2958
Size: 649 784 644 881
Fragments arranged by size:
881 784 649 644
Bsp1286I G_dGCh'C
Cuts at: 0 592 649 749 1255 1433 1530 2077 2300
Size: 592 57 100 506 178 97 547 223
Cuts at: 2300 2349 2958
Size: 49 609
Fragments arranged by size:
609 592 547 506 223 178 100 97
57 49
BspEI T'CCGG_A
Cuts at: 0 1048 2958
Size: 1048 1910
BspHI T'CATG_A
Cuts at: 0 467 2958
Size: 467 2491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1199 1863 2711 2958
Size: 1199 664 848 247
Fragments arranged by size:
1199 848 664 247
BsrBI CCG'CTC
Cuts at: 0 2472 2958
Size: 2472 486
BsrDI GCAATG_nn'
Cuts at: 0 419 924 1664 2958
Size: 419 505 740 1294
Fragments arranged by size:
1294 740 505 419
BsrFI r'CCGG_y
Cuts at: 0 1498 2606 2809 2958
Size: 1498 1108 203 149
Fragments arranged by size:
1498 1108 203 149
BsrGI T'GTAC_A
Cuts at: 0 1134 2958
Size: 1134 1824
BstAPI GCAn_nnn'nTGC
Cuts at: 0 803 1121 2958
Size: 803 318 1837
Fragments arranged by size:
1837 803 318
BstEII G'GTnAC_C
Cuts at: 0 1476 2958
Size: 1476 1482
BstXI CCAn_nnnn'nTGG
Cuts at: 0 125 390 541 1387 2349 2778 2958
Size: 125 265 151 846 962 429 180
Fragments arranged by size:
962 846 429 265 180 151 125
BstYI r'GATC_y
Cuts at: 0 1220 1781 1940 2360 2958
Size: 1220 561 159 420 598
Fragments arranged by size:
1220 598 561 420 159
BtgI C'CryG_G
Cuts at: 0 53 240 400 1343 1535 2543 2675 2958
Size: 53 187 160 943 192 1008 132 283
Fragments arranged by size:
1008 943 283 192 187 160 132 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 546 635 663 1665 2172 2958
Size: 546 89 28 1002 507 786
Fragments arranged by size:
1002 786 546 507 89 28
BtsI GCAGTG_nn'
Cuts at: 0 1319 1829 1914 2958
Size: 1319 510 85 1044
Fragments arranged by size:
1319 1044 510 85
ClaI AT'CG_AT
Cuts at: 0 1790 2958
Size: 1790 1168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1074 1109 2958
Size: 1074 35 1849
Fragments arranged by size:
1849 1074 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1965 2958
Size: 1965 993
EaeI y'GGCC_r
Cuts at: 0 365 459 1643 1758 1911 1949 2414 2619
Size: 365 94 1184 115 153 38 465 205
Cuts at: 2619 2883 2958
Size: 264 75
Fragments arranged by size:
1184 465 365 264 205 153 115 94
75 38
EagI C'GGCC_G
Cuts at: 0 1643 2958
Size: 1643 1315
EarI CTCTTCn'nnn_
Cuts at: 0 125 686 2958
Size: 125 561 2272
Fragments arranged by size:
2272 561 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2369 2958
Size: 2369 589
EcoICRI GAG'CTC
Cuts at: 0 1431 2958
Size: 1431 1527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1045 1939 2074 2785 2958
Size: 911 134 894 135 711 173
Fragments arranged by size:
911 894 711 173 135 134
EcoNI CCTnn'n_nnAGG
Cuts at: 0 119 2958
Size: 119 2839
EcoO109I rG'GnC_Cy
Cuts at: 0 1062 1488 1809 2958
Size: 1062 426 321 1149
Fragments arranged by size:
1149 1062 426 321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 345 1587 2814 2958
Size: 345 1242 1227 144
Fragments arranged by size:
1242 1227 345 144
HaeII r_GCGC'y
Cuts at: 0 240 248 614 759 1505 1554 1992 2561
Size: 240 8 366 145 746 49 438 569
Cuts at: 2561 2683 2958
Size: 122 275
Fragments arranged by size:
746 569 438 366 275 240 145 122
49 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 346 378 381 413 634 666
Size: 96 32 218 32 3 32 221 32
Cuts at: 666 2308 2340 2443 2475 2640 2672 2818 2850
Size: 1642 32 103 32 165 32 146 32
Cuts at: 2850 2958
Size: 108
Fragments arranged by size:
1642 221 218 165 146 108 103 96
32 32 32 32 32 32 32 32
3
HincII GTy'rAC
Cuts at: 0 2262 2631 2958
Size: 2262 369 327
Fragments arranged by size:
2262 369 327
KasI G'GCGC_C
Cuts at: 0 236 244 610 1501 2679 2958
Size: 236 8 366 891 1178 279
Fragments arranged by size:
1178 891 366 279 236 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2267 2958
Size: 2267 691
MscI TGG'CCA
Cuts at: 0 1760 1913 1951 2885 2958
Size: 1760 153 38 934 73
Fragments arranged by size:
1760 934 153 73 38
MslI CAynn'nnrTG
Cuts at: 0 123 259 484 565 1669 2080 2443 2776
Size: 123 136 225 81 1104 411 363 333
Cuts at: 2776 2958
Size: 182
Fragments arranged by size:
1104 411 363 333 225 182 136 123
81
MspA1I CmG'CkG
Cuts at: 0 42 515 674 1121 1559 1648 2166 2194
Size: 42 473 159 447 438 89 518 28
Cuts at: 2194 2372 2709 2958
Size: 178 337 249
Fragments arranged by size:
518 473 447 438 337 249 178 159
89 42 28
NaeI GCC'GGC
Cuts at: 0 1500 2811 2958
Size: 1500 1311 147
Fragments arranged by size:
1500 1311 147
NarI GG'CG_CC
Cuts at: 0 237 245 611 1502 2680 2958
Size: 237 8 366 891 1178 278
Fragments arranged by size:
1178 891 366 278 237 8
NcoI C'CATG_G
Cuts at: 0 400 2543 2675 2958
Size: 400 2143 132 283
Fragments arranged by size:
2143 400 283 132
NgoMIV G'CCGG_C
Cuts at: 0 1498 2809 2958
Size: 1498 1311 149
Fragments arranged by size:
1498 1311 149
NsiI A_TGCA'T
Cuts at: 0 1328 2958
Size: 1328 1630
NspI r_CATG'y
Cuts at: 0 555 765 957 2178 2958
Size: 555 210 192 1221 780
Fragments arranged by size:
1221 780 555 210 192
PasI CC'CwG_GG
Cuts at: 0 2349 2958
Size: 2349 609
PflMI CCAn_nnn'nTGG
Cuts at: 0 2169 2372 2958
Size: 2169 203 586
Fragments arranged by size:
2169 586 203
PfoI T'CCnGG_A
Cuts at: 0 533 620 1397 1610 2958
Size: 533 87 777 213 1348
Fragments arranged by size:
1348 777 533 213 87
PmlI CAC'GTG
Cuts at: 0 261 2958
Size: 261 2697
PpuMI rG'GwC_Cy
Cuts at: 0 1062 1809 2958
Size: 1062 747 1149
Fragments arranged by size:
1149 1062 747
PshAI GACnn'nnGTC
Cuts at: 0 504 2958
Size: 504 2454
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2389 2421 2958
Size: 2389 32 537
Fragments arranged by size:
2389 537 32
PstI C_TGCA'G
Cuts at: 0 292 1415 1909 2958
Size: 292 1123 494 1049
Fragments arranged by size:
1123 1049 494 292
PvuII CAG'CTG
Cuts at: 0 674 2166 2194 2958
Size: 674 1492 28 764
Fragments arranged by size:
1492 764 674 28
SacI G_AGCT'C
Cuts at: 0 1433 2958
Size: 1433 1525
ScaI AGT'ACT
Cuts at: 0 1417 2958
Size: 1417 1541
SexAI A'CCwGG_T
Cuts at: 0 176 2270 2958
Size: 176 2094 688
Fragments arranged by size:
2094 688 176
SfcI C'TryA_G
Cuts at: 0 80 288 927 1411 1554 1905 2958
Size: 80 208 639 484 143 351 1053
Fragments arranged by size:
1053 639 484 351 208 143 80
SfoI GGC'GCC
Cuts at: 0 238 246 612 1503 2681 2958
Size: 238 8 366 891 1178 277
Fragments arranged by size:
1178 891 366 277 238 8
SmaI CCC'GGG
Cuts at: 0 667 2958
Size: 667 2291
SmlI C'TyrA_G
Cuts at: 0 1963 2958
Size: 1963 995
SphI G_CATG'C
Cuts at: 0 2178 2958
Size: 2178 780
StuI AGG'CCT
Cuts at: 0 853 1160 2958
Size: 853 307 1798
Fragments arranged by size:
1798 853 307
StyI C'CwwG_G
Cuts at: 0 400 840 1838 2543 2675 2958
Size: 400 440 998 705 132 283
Fragments arranged by size:
998 705 440 400 283 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 393 445 1180 1219 2958
Size: 393 52 735 39 1739
Fragments arranged by size:
1739 735 393 52 39
TatI w'GTAC_w
Cuts at: 0 1134 1208 1415 1439 1568 1727 2786 2958
Size: 1134 74 207 24 129 159 1059 172
Fragments arranged by size:
1134 1059 207 172 159 129 74 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 721 753 2958
Size: 721 32 2205
Fragments arranged by size:
2205 721 32
Tth111I GACn'n_nGTC
Cuts at: 0 1066 2958
Size: 1066 1892
XbaI T'CTAG_A
Cuts at: 0 2310 2958
Size: 2310 648
XmaI C'CCGG_G
Cuts at: 0 665 2958
Size: 665 2293
ZraI GAC'GTC
Cuts at: 0 2829 2958
Size: 2829 129
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AlwNI AvaI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PflMI PfoI PmlI PpuMI PshAI PsrI PstI PvuII
SacI ScaI SexAI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BmgBI BmtI BsiWI BsmI BsmBI BssHII BssSI BstBI
BstZ17I Bsu36I DraI DraIII EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI PacI PciI PmeI PpiI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XcmI XhoI XmnI