(Linear) (Six-base) MAPSORT of: KIBB1483.seq Check: 7893 from: 1 to: 3594 KIBB1483.orf With 182 enzymes: SgfI * October 24, 2006 15:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1545 1966 2292 3594 Size: 1545 421 326 1302 Fragments arranged by size: 1545 1302 421 326 AatII G_ACGT'C Cuts at: 0 1421 3589 3594 Size: 1421 2168 5 Fragments arranged by size: 2168 1421 5 AccI GT'mk_AC Cuts at: 0 1169 2744 3594 Size: 1169 1575 850 Fragments arranged by size: 1575 1169 850 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1624 2599 3594 Size: 1624 975 995 Fragments arranged by size: 1624 995 975 AflIII A'CryG_T Cuts at: 0 2618 3594 Size: 2618 976 AleI CACnn'nnGTG Cuts at: 0 1126 2392 2968 3594 Size: 1126 1266 576 626 Fragments arranged by size: 1266 1126 626 576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1651 2986 3594 Size: 1651 1335 608 Fragments arranged by size: 1651 1335 608 AlwNI CAG_nnn'CTG Cuts at: 0 2389 3249 3594 Size: 2389 860 345 Fragments arranged by size: 2389 860 345 ApaLI G'TGCA_C Cuts at: 0 75 1158 3594 Size: 75 1083 2436 Fragments arranged by size: 2436 1083 75 ApoI r'AATT_y Cuts at: 0 239 571 2494 3379 3594 Size: 239 332 1923 885 215 Fragments arranged by size: 1923 885 332 239 215 AvaI C'yCGr_G Cuts at: 0 1231 2078 2134 3462 3594 Size: 1231 847 56 1328 132 Fragments arranged by size: 1328 1231 847 132 56 BamHI G'GATC_C Cuts at: 0 2159 3272 3594 Size: 2159 1113 322 Fragments arranged by size: 2159 1113 322 BanI G'GyrC_C Cuts at: 0 173 208 1540 2551 2977 3594 Size: 173 35 1332 1011 426 617 Fragments arranged by size: 1332 1011 617 426 173 35 BanII G_rGCy'C Cuts at: 0 92 802 1241 1622 1853 1871 3089 3191 Size: 92 710 439 381 231 18 1218 102 Cuts at: 3191 3469 3568 3594 Size: 278 99 26 Fragments arranged by size: 1218 710 439 381 278 231 102 99 92 26 18 BbeI G_GCGC'C Cuts at: 0 177 1544 2555 3594 Size: 177 1367 1011 1039 Fragments arranged by size: 1367 1039 1011 177 BbsI GAAGACnn'nnnn_ Cuts at: 0 1797 3594 Size: 1797 1797 BbvCI CC'TCA_GC Cuts at: 0 2671 3594 Size: 2671 923 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 382 416 2323 2357 3594 Size: 382 34 1907 34 1237 Fragments arranged by size: 1907 1237 382 34 34 BclI T'GATC_A Cuts at: 0 1091 1478 3594 Size: 1091 387 2116 Fragments arranged by size: 2116 1091 387 BglI GCCn_nnn'nGGC Cuts at: 0 62 1236 1586 2138 2404 2632 3594 Size: 62 1174 350 552 266 228 962 Fragments arranged by size: 1174 962 552 350 266 228 62 BglII A'GATC_T Cuts at: 0 2204 3594 Size: 2204 1390 Bme1580I G_kGCm'C Cuts at: 0 79 118 211 1162 1232 2980 3594 Size: 79 39 93 951 70 1748 614 Fragments arranged by size: 1748 951 614 93 79 70 39 BmgBI CAC'GTC Cuts at: 0 315 2361 2394 3201 3594 Size: 315 2046 33 807 393 Fragments arranged by size: 2046 807 393 315 33 BmrI ACTGGGnnnn_n' Cuts at: 0 1932 3594 Size: 1932 1662 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 111 3594 Size: 111 3483 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 352 537 746 2691 3594 Size: 200 152 185 209 1945 903 Fragments arranged by size: 1945 903 209 200 185 152 Bpu10I CC'TnA_GC Cuts at: 0 801 2671 3594 Size: 801 1870 923 Fragments arranged by size: 1870 923 801 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 685 934 1744 2332 3115 3124 3436 3594 Size: 685 249 810 588 783 9 312 158 Fragments arranged by size: 810 783 685 588 312 249 158 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 1321 2653 3594 Size: 1321 1332 941 Fragments arranged by size: 1332 1321 941 BsaAI yAC'GTr Cuts at: 0 789 1173 3594 Size: 789 384 2421 Fragments arranged by size: 2421 789 384 BsaBI GATnn'nnATC Cuts at: 0 2998 3594 Size: 2998 596 BsaHI Gr'CG_yC Cuts at: 0 174 1418 1541 2552 3586 3594 Size: 174 1244 123 1011 1034 8 Fragments arranged by size: 1244 1034 1011 174 123 8 BsaWI w'CCGG_w Cuts at: 0 2945 3383 3594 Size: 2945 438 211 Fragments arranged by size: 2945 438 211 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 13 43 1432 1462 1636 1666 2473 2503 Size: 13 30 1389 30 174 30 807 30 Cuts at: 2503 3074 3104 3594 Size: 571 30 490 Fragments arranged by size: 1389 807 571 490 174 30 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 100 463 652 1657 2226 3184 3196 3199 Size: 100 363 189 1005 569 958 12 3 Cuts at: 3199 3220 3594 Size: 21 374 Fragments arranged by size: 1005 958 569 374 363 189 100 21 12 3 BseYI C'CCAG_C Cuts at: 0 1444 1584 1903 2973 3398 3594 Size: 1444 140 319 1070 425 196 Fragments arranged by size: 1444 1070 425 319 196 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1069 1195 2327 2608 2946 3025 3594 Size: 1069 126 1132 281 338 79 569 Fragments arranged by size: 1132 1069 569 338 281 126 79 BsiEI CG_ry'CG Cuts at: 0 1821 2688 3527 3594 Size: 1821 867 839 67 Fragments arranged by size: 1821 867 839 67 BsiHKAI G_wGCw'C Cuts at: 0 79 92 1106 1162 1696 1760 3089 3191 Size: 79 13 1014 56 534 64 1329 102 Cuts at: 3191 3594 Size: 403 Fragments arranged by size: 1329 1014 534 403 102 79 64 56 13 BsiWI C'GTAC_G Cuts at: 0 2681 3594 Size: 2681 913 BsmI GAATG_Cn' Cuts at: 0 2826 3594 Size: 2826 768 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3577 3594 Size: 3577 17 Bsp1286I G_dGCh'C Cuts at: 0 79 92 118 211 802 1106 1162 1232 Size: 79 13 26 93 591 304 56 70 Cuts at: 1232 1241 1622 1696 1760 1853 1871 2980 3089 Size: 9 381 74 64 93 18 1109 109 Cuts at: 3089 3191 3469 3568 3594 Size: 102 278 99 26 Fragments arranged by size: 1109 591 381 304 278 109 102 99 93 93 79 74 70 64 56 26 26 18 13 9 BspEI T'CCGG_A Cuts at: 0 2945 3383 3594 Size: 2945 438 211 Fragments arranged by size: 2945 438 211 BspHI T'CATG_A Cuts at: 0 2873 3594 Size: 2873 721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 436 1545 1966 2292 3594 Size: 436 1109 421 326 1302 Fragments arranged by size: 1302 1109 436 421 326 BsrBI CCG'CTC Cuts at: 0 95 214 2700 3225 3594 Size: 95 119 2486 525 369 Fragments arranged by size: 2486 525 369 119 95 BsrDI GCAATG_nn' Cuts at: 0 699 1344 2931 3594 Size: 699 645 1587 663 Fragments arranged by size: 1587 699 663 645 BsrFI r'CCGG_y Cuts at: 0 170 3594 Size: 170 3424 BsrGI T'GTAC_A Cuts at: 0 1652 1661 1730 3594 Size: 1652 9 69 1864 Fragments arranged by size: 1864 1652 69 9 BssSI C'ACGA_G Cuts at: 0 292 1799 3594 Size: 292 1507 1795 Fragments arranged by size: 1795 1507 292 BstAPI GCAn_nnn'nTGC Cuts at: 0 1880 3594 Size: 1880 1714 BstEII G'GTnAC_C Cuts at: 0 655 3594 Size: 655 2939 BstXI CCAn_nnnn'nTGG Cuts at: 0 1263 1467 2973 3594 Size: 1263 204 1506 621 Fragments arranged by size: 1506 1263 621 204 BstYI r'GATC_y Cuts at: 0 605 1433 2159 2204 2909 3173 3245 3272 Size: 605 828 726 45 705 264 72 27 Cuts at: 3272 3284 3594 Size: 12 310 Fragments arranged by size: 828 726 705 605 310 264 72 45 27 12 BtgI C'CryG_G Cuts at: 0 57 578 752 932 1144 1331 1466 1853 Size: 57 521 174 180 212 187 135 387 Cuts at: 1853 2372 2654 2913 3594 Size: 519 282 259 681 Fragments arranged by size: 681 521 519 387 282 259 212 187 180 174 135 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1473 1877 2070 2364 3090 3594 Size: 1473 404 193 294 726 504 Fragments arranged by size: 1473 726 504 404 294 193 BtsI GCAGTG_nn' Cuts at: 0 2462 3037 3594 Size: 2462 575 557 Fragments arranged by size: 2462 575 557 EaeI y'GGCC_r Cuts at: 0 591 1247 1993 2209 2309 2396 2528 2639 Size: 591 656 746 216 100 87 132 111 Cuts at: 2639 2685 3594 Size: 46 909 Fragments arranged by size: 909 746 656 591 216 132 111 100 87 46 EagI C'GGCC_G Cuts at: 0 2685 3594 Size: 2685 909 EarI CTCTTCn'nnn_ Cuts at: 0 685 875 881 2890 3555 3594 Size: 685 190 6 2009 665 39 Fragments arranged by size: 2009 685 665 190 39 6 EcoICRI GAG'CTC Cuts at: 0 90 3087 3189 3594 Size: 90 2997 102 405 Fragments arranged by size: 2997 405 102 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 352 537 746 1624 2599 2691 3594 Size: 200 152 185 209 878 975 92 903 Fragments arranged by size: 975 903 878 209 200 185 152 92 EcoNI CCTnn'n_nnAGG Cuts at: 0 194 1606 3142 3594 Size: 194 1412 1536 452 Fragments arranged by size: 1536 1412 452 194 EcoO109I rG'GnC_Cy Cuts at: 0 540 762 1983 2001 3594 Size: 540 222 1221 18 1593 Fragments arranged by size: 1593 1221 540 222 18 EcoRI G'AATT_C Cuts at: 0 3379 3594 Size: 3379 215 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1083 3594 Size: 1083 2511 FspI TGC'GCA Cuts at: 0 745 1627 1681 3594 Size: 745 882 54 1913 Fragments arranged by size: 1913 882 745 54 HaeII r_GCGC'y Cuts at: 0 177 307 1505 1544 2555 3500 3594 Size: 177 130 1198 39 1011 945 94 Fragments arranged by size: 1198 1011 945 177 130 94 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 13 45 700 732 1368 1400 1432 1464 Size: 13 32 655 32 636 32 32 32 Cuts at: 1464 2473 2505 3594 Size: 1009 32 1089 Fragments arranged by size: 1089 1009 655 636 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 0 1134 2520 3594 Size: 1134 1386 1074 Fragments arranged by size: 1386 1134 1074 KasI G'GCGC_C Cuts at: 0 173 1540 2551 3594 Size: 173 1367 1011 1043 Fragments arranged by size: 1367 1043 1011 173 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 430 3594 Size: 430 3164 MscI TGG'CCA Cuts at: 0 593 2211 2398 2641 3594 Size: 593 1618 187 243 953 Fragments arranged by size: 1618 953 593 243 187 MslI CAynn'nnrTG Cuts at: 0 937 1087 1099 1126 1204 1316 1393 1966 Size: 937 150 12 27 78 112 77 573 Cuts at: 1966 2392 2968 3594 Size: 426 576 626 Fragments arranged by size: 937 626 576 573 426 150 112 78 77 27 12 MspA1I CmG'CkG Cuts at: 0 2028 2298 2807 3029 3045 3594 Size: 2028 270 509 222 16 549 Fragments arranged by size: 2028 549 509 270 222 16 NaeI GCC'GGC Cuts at: 0 172 3594 Size: 172 3422 NarI GG'CG_CC Cuts at: 0 174 1541 2552 3594 Size: 174 1367 1011 1042 Fragments arranged by size: 1367 1042 1011 174 NcoI C'CATG_G Cuts at: 0 57 932 1331 1466 1853 2372 3594 Size: 57 875 399 135 387 519 1222 Fragments arranged by size: 1222 875 519 399 387 135 57 NgoMIV G'CCGG_C Cuts at: 0 170 3594 Size: 170 3424 NspI r_CATG'y Cuts at: 0 999 1731 3594 Size: 999 732 1863 Fragments arranged by size: 1863 999 732 PasI CC'CwG_GG Cuts at: 0 354 411 3594 Size: 354 57 3183 Fragments arranged by size: 3183 354 57 PflMI CCAn_nnn'nTGG Cuts at: 0 792 3579 3594 Size: 792 2787 15 Fragments arranged by size: 2787 792 15 PfoI T'CCnGG_A Cuts at: 0 329 2707 3176 3594 Size: 329 2378 469 418 Fragments arranged by size: 2378 469 418 329 PmlI CAC'GTG Cuts at: 0 789 3594 Size: 789 2805 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 517 549 2776 2808 3594 Size: 517 32 2227 32 786 Fragments arranged by size: 2227 786 517 32 32 PpuMI rG'GwC_Cy Cuts at: 0 762 1983 2001 3594 Size: 762 1221 18 1593 Fragments arranged by size: 1593 1221 762 18 PspXI vC'TCGA_Gb Cuts at: 0 2134 3462 3594 Size: 2134 1328 132 Fragments arranged by size: 2134 1328 132 PstI C_TGCA'G Cuts at: 0 290 311 548 1025 3044 3594 Size: 290 21 237 477 2019 550 Fragments arranged by size: 2019 550 477 290 237 21 PvuII CAG'CTG Cuts at: 0 3029 3045 3594 Size: 3029 16 549 Fragments arranged by size: 3029 549 16 RsrII CG'GwC_CG Cuts at: 0 2043 3594 Size: 2043 1551 SacI G_AGCT'C Cuts at: 0 92 3089 3191 3594 Size: 92 2997 102 403 Fragments arranged by size: 2997 403 102 92 SapI GCTCTTCn'nnn_ Cuts at: 0 2890 3594 Size: 2890 704 ScaI AGT'ACT Cuts at: 0 1180 3594 Size: 1180 2414 SexAI A'CCwGG_T Cuts at: 0 737 3594 Size: 737 2857 SfcI C'TryA_G Cuts at: 0 286 307 544 1021 3040 3594 Size: 286 21 237 477 2019 554 Fragments arranged by size: 2019 554 477 286 237 21 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2404 3594 Size: 2404 1190 SfoI GGC'GCC Cuts at: 0 175 1542 2553 3594 Size: 175 1367 1011 1041 Fragments arranged by size: 1367 1041 1011 175 SmlI C'TyrA_G Cuts at: 0 700 949 1759 2134 2347 3130 3139 3451 Size: 700 249 810 375 213 783 9 312 Cuts at: 3451 3462 3594 Size: 11 132 Fragments arranged by size: 810 783 700 375 312 249 213 132 11 9 SphI G_CATG'C Cuts at: 0 999 3594 Size: 999 2595 StuI AGG'CCT Cuts at: 0 1405 2407 3263 3594 Size: 1405 1002 856 331 Fragments arranged by size: 1405 1002 856 331 StyI C'CwwG_G Cuts at: 0 57 932 1331 1466 1853 2372 2399 3594 Size: 57 875 399 135 387 519 27 1195 Fragments arranged by size: 1195 875 519 399 387 135 57 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 817 2589 3541 3594 Size: 817 1772 952 53 Fragments arranged by size: 1772 952 817 53 TatI w'GTAC_w Cuts at: 0 1178 1652 1661 1730 3594 Size: 1178 474 9 69 1864 Fragments arranged by size: 1864 1178 474 69 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 773 805 2113 2145 3594 Size: 773 32 1308 32 1449 Fragments arranged by size: 1449 1308 773 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 351 649 3576 3594 Size: 351 298 2927 18 Fragments arranged by size: 2927 351 298 18 XhoI C'TCGA_G Cuts at: 0 2134 3462 3594 Size: 2134 1328 132 Fragments arranged by size: 2134 1328 132 XmnI GAAnn'nnTTC Cuts at: 0 532 2791 3022 3301 3594 Size: 532 2259 231 279 293 Fragments arranged by size: 2259 532 293 279 231 ZraI GAC'GTC Cuts at: 0 1419 3587 3594 Size: 1419 2168 7 Fragments arranged by size: 2168 1419 7 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspXI PstI PvuII RsrII SacI SapI ScaI SexAI SfcI SfiI SfoI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BlpI BmtI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspOMI PsrI PvuI SacII SalI SanDI SbfI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI