(Linear) (Six-base) MAPSORT of: KIBB1488.seq Check: 5892 from: 1 to: 5232 KIBB1488.orf With 182 enzymes: SgfI * October 24, 2006 15:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2510 2646 5232 Size: 2510 136 2586 Fragments arranged by size: 2586 2510 136 AatII G_ACGT'C Cuts at: 0 4463 5232 Size: 4463 769 Acc65I G'GTAC_C Cuts at: 0 470 5232 Size: 470 4762 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1268 1382 1915 2752 4153 4775 5232 Size: 1268 114 533 837 1401 622 457 Fragments arranged by size: 1401 1268 837 622 533 457 114 AfeI AGC'GCT Cuts at: 0 2101 5232 Size: 2101 3131 AflIII A'CryG_T Cuts at: 0 2122 2603 5232 Size: 2122 481 2629 Fragments arranged by size: 2629 2122 481 AhdI GACnn_n'nnGTC Cuts at: 0 360 5232 Size: 360 4872 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1738 1770 5232 Size: 1738 32 3462 Fragments arranged by size: 3462 1738 32 AlwNI CAG_nnn'CTG Cuts at: 0 1005 1195 1345 2661 3227 3700 5103 5232 Size: 1005 190 150 1316 566 473 1403 129 Fragments arranged by size: 1403 1316 1005 566 473 190 150 129 ApaI G_GGCC'C Cuts at: 0 1146 4559 5232 Size: 1146 3413 673 Fragments arranged by size: 3413 1146 673 ApaLI G'TGCA_C Cuts at: 0 4396 5232 Size: 4396 836 ApoI r'AATT_y Cuts at: 0 2758 5232 Size: 2758 2474 AseI AT'TA_AT Cuts at: 0 2060 5232 Size: 2060 3172 AvaI C'yCGr_G Cuts at: 0 140 504 1408 1532 2744 3020 3115 4595 Size: 140 364 904 124 1212 276 95 1480 Cuts at: 4595 4684 5083 5232 Size: 89 399 149 Fragments arranged by size: 1480 1212 904 399 364 276 149 140 124 95 89 BamHI G'GATC_C Cuts at: 0 3164 5232 Size: 3164 2068 BanI G'GyrC_C Cuts at: 0 264 416 470 762 1611 1729 2664 2703 Size: 264 152 54 292 849 118 935 39 Cuts at: 2703 3565 3612 4362 4713 5222 5232 Size: 862 47 750 351 509 10 Fragments arranged by size: 935 862 849 750 509 351 292 264 152 118 54 47 39 10 BanII G_rGCy'C Cuts at: 0 857 1070 1146 1565 1718 2192 2271 2677 Size: 857 213 76 419 153 474 79 406 Cuts at: 2677 3560 3694 4559 5232 Size: 883 134 865 673 Fragments arranged by size: 883 865 857 673 474 419 406 213 153 134 79 76 BbeI G_GCGC'C Cuts at: 0 3616 4366 5232 Size: 3616 750 866 Fragments arranged by size: 3616 866 750 BbsI GAAGACnn'nnnn_ Cuts at: 0 883 1220 2023 3456 4907 5232 Size: 883 337 803 1433 1451 325 Fragments arranged by size: 1451 1433 883 803 337 325 BbvCI CC'TCA_GC Cuts at: 0 4772 5232 Size: 4772 460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4434 4468 4997 5031 5232 Size: 4434 34 529 34 201 Fragments arranged by size: 4434 529 201 34 34 BclI T'GATC_A Cuts at: 0 5051 5232 Size: 5051 181 BglI GCCn_nnn'nGGC Cuts at: 0 1795 2255 3586 4638 5232 Size: 1795 460 1331 1052 594 Fragments arranged by size: 1795 1331 1052 594 460 BglII A'GATC_T Cuts at: 0 641 5232 Size: 641 4591 BlpI GC'TnA_GC Cuts at: 0 1717 5232 Size: 1717 3515 Bme1580I G_kGCm'C Cuts at: 0 140 269 419 1146 1929 2708 3570 4400 Size: 140 129 150 727 783 779 862 830 Cuts at: 4400 4559 4716 4786 5184 5232 Size: 159 157 70 398 48 Fragments arranged by size: 862 830 783 779 727 398 159 157 150 140 129 70 48 BmgBI CAC'GTC Cuts at: 0 2125 4218 4563 5232 Size: 2125 2093 345 669 Fragments arranged by size: 2125 2093 669 345 BmrI ACTGGGnnnn_n' Cuts at: 0 681 1651 2464 2605 3696 5091 5232 Size: 681 970 813 141 1091 1395 141 Fragments arranged by size: 1395 1091 970 813 681 141 141 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3035 5154 5232 Size: 3035 2119 78 Fragments arranged by size: 3035 2119 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 244 916 982 988 1025 1732 2293 Size: 112 132 672 66 6 37 707 561 Cuts at: 2293 2370 2566 3692 4081 4522 4825 5185 5232 Size: 77 196 1126 389 441 303 360 47 Fragments arranged by size: 1126 707 672 561 441 389 360 303 196 132 112 77 66 47 37 6 Bpu10I CC'TnA_GC Cuts at: 0 226 2431 4772 5232 Size: 226 2205 2341 460 Fragments arranged by size: 2341 2205 460 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3030 5232 Size: 3030 2202 BsaI GGTCTCn'nnnn_ Cuts at: 0 1590 1643 1964 3112 3982 4540 4924 5232 Size: 1590 53 321 1148 870 558 384 308 Fragments arranged by size: 1590 1148 870 558 384 321 308 53 BsaAI yAC'GTr Cuts at: 0 2414 2604 4533 5232 Size: 2414 190 1929 699 Fragments arranged by size: 2414 1929 699 190 BsaHI Gr'CG_yC Cuts at: 0 962 3613 4363 4460 5232 Size: 962 2651 750 97 772 Fragments arranged by size: 2651 962 772 750 97 BsaWI w'CCGG_w Cuts at: 0 1742 5232 Size: 1742 3490 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2378 2408 2608 2638 2762 2792 3096 3126 Size: 2378 30 200 30 124 30 304 30 Cuts at: 3126 3720 3750 4060 4090 4372 4402 5232 Size: 594 30 310 30 282 30 830 Fragments arranged by size: 2378 830 594 310 304 282 200 124 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 241 295 393 1072 1436 2302 2839 3728 Size: 241 54 98 679 364 866 537 889 Cuts at: 3728 3982 4078 4081 4393 5143 5232 Size: 254 96 3 312 750 89 Fragments arranged by size: 889 866 750 679 537 364 312 254 241 98 96 89 54 3 BseYI C'CCAG_C Cuts at: 0 1188 1690 1800 2632 2651 3326 3553 3771 Size: 1188 502 110 832 19 675 227 218 Cuts at: 3771 5183 5232 Size: 1412 49 Fragments arranged by size: 1412 1188 832 675 502 227 218 110 49 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 847 2188 2488 3500 3793 4642 4666 Size: 142 705 1341 300 1012 293 849 24 Cuts at: 4666 4936 5232 Size: 270 296 Fragments arranged by size: 1341 1012 849 705 300 296 293 270 142 24 BsiEI CG_ry'CG Cuts at: 0 1432 5232 Size: 1432 3800 BsiHKAI G_wGCw'C Cuts at: 0 511 1070 1718 3106 4400 5161 5232 Size: 511 559 648 1388 1294 761 71 Fragments arranged by size: 1388 1294 761 648 559 511 71 BsmI GAATG_Cn' Cuts at: 0 1199 1295 5232 Size: 1199 96 3937 Fragments arranged by size: 3937 1199 96 Bsp1286I G_dGCh'C Cuts at: 0 140 269 419 511 857 1070 1146 1565 Size: 140 129 150 92 346 213 76 419 Cuts at: 1565 1718 1929 2192 2271 2677 2708 3106 3560 Size: 153 211 263 79 406 31 398 454 Cuts at: 3560 3570 3694 4400 4559 4716 4786 5161 5184 Size: 10 124 706 159 157 70 375 23 Cuts at: 5184 5232 Size: 48 Fragments arranged by size: 706 454 419 406 398 375 346 263 213 211 159 157 153 150 140 129 124 92 79 76 70 48 31 23 10 BspHI T'CATG_A Cuts at: 0 1550 5232 Size: 1550 3682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 401 2510 2646 3264 3632 4186 5111 5232 Size: 401 2109 136 618 368 554 925 121 Fragments arranged by size: 2109 925 618 554 401 368 136 121 BsrBI CCG'CTC Cuts at: 0 1283 1428 4809 5232 Size: 1283 145 3381 423 Fragments arranged by size: 3381 1283 423 145 BsrDI GCAATG_nn' Cuts at: 0 3408 3662 3720 5232 Size: 3408 254 58 1512 Fragments arranged by size: 3408 1512 254 58 BsrFI r'CCGG_y Cuts at: 0 4632 4650 5232 Size: 4632 18 582 Fragments arranged by size: 4632 582 18 BsrGI T'GTAC_A Cuts at: 0 572 5232 Size: 572 4660 BssSI C'ACGA_G Cuts at: 0 1448 3823 5232 Size: 1448 2375 1409 Fragments arranged by size: 2375 1448 1409 BstAPI GCAn_nnn'nTGC Cuts at: 0 3816 4629 5179 5232 Size: 3816 813 550 53 Fragments arranged by size: 3816 813 550 53 BstBI TT'CG_AA Cuts at: 0 2756 5232 Size: 2756 2476 BstXI CCAn_nnnn'nTGG Cuts at: 0 635 2342 3670 3779 4374 5209 5232 Size: 635 1707 1328 109 595 835 23 Fragments arranged by size: 1707 1328 835 635 595 109 23 BstYI r'GATC_y Cuts at: 0 641 1107 2276 2420 2949 3138 3164 5232 Size: 641 466 1169 144 529 189 26 2068 Fragments arranged by size: 2068 1169 641 529 466 189 144 26 Bsu36I CC'TnA_GG Cuts at: 0 317 5232 Size: 317 4915 BtgI C'CryG_G Cuts at: 0 343 1220 1484 1644 2424 2708 3134 5232 Size: 343 877 264 160 780 284 426 2098 Fragments arranged by size: 2098 877 780 426 343 284 264 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3423 4437 5232 Size: 3423 1014 795 Fragments arranged by size: 3423 1014 795 BtsI GCAGTG_nn' Cuts at: 0 1497 3103 3106 5232 Size: 1497 1606 3 2126 Fragments arranged by size: 2126 1606 1497 3 DraIII CAC_nnn'GTG Cuts at: 0 1134 1659 3135 4409 4745 5232 Size: 1134 525 1476 1274 336 487 Fragments arranged by size: 1476 1274 1134 525 487 336 DrdI GACnn_nn'nnGTC Cuts at: 0 2003 5232 Size: 2003 3229 EaeI y'GGCC_r Cuts at: 0 456 1284 1429 1661 2462 3458 4448 4652 Size: 456 828 145 232 801 996 990 204 Cuts at: 4652 4736 5232 Size: 84 496 Fragments arranged by size: 996 990 828 801 496 456 232 204 145 84 EagI C'GGCC_G Cuts at: 0 1429 5232 Size: 1429 3803 EarI CTCTTCn'nnn_ Cuts at: 0 277 403 2227 5232 Size: 277 126 1824 3005 Fragments arranged by size: 3005 1824 277 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2500 2518 2654 3199 4434 4909 5232 Size: 2500 18 136 545 1235 475 323 Fragments arranged by size: 2500 1235 545 475 323 136 18 EcoICRI GAG'CTC Cuts at: 0 1068 1716 5232 Size: 1068 648 3516 Fragments arranged by size: 3516 1068 648 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 244 916 982 988 1025 1268 1382 Size: 112 132 672 66 6 37 243 114 Cuts at: 1382 1732 1915 2293 2370 2566 2752 3692 4081 Size: 350 183 378 77 196 186 940 389 Cuts at: 4081 4153 4522 4775 4825 5185 5232 Size: 72 369 253 50 360 47 Fragments arranged by size: 940 672 389 378 369 360 350 253 243 196 186 183 132 114 112 77 72 66 50 47 37 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 862 943 1156 1400 2284 4063 5232 Size: 862 81 213 244 884 1779 1169 Fragments arranged by size: 1779 1169 884 862 244 213 81 EcoO109I rG'GnC_Cy Cuts at: 0 102 313 632 1142 1143 1405 1571 1694 Size: 102 211 319 510 1 262 166 123 Cuts at: 1694 2292 2750 2978 3252 3354 3768 4137 4185 Size: 598 458 228 274 102 414 369 48 Cuts at: 4185 4555 4556 5232 Size: 370 1 676 Fragments arranged by size: 676 598 510 458 414 370 369 319 274 262 228 211 166 123 102 102 48 1 1 EcoRI G'AATT_C Cuts at: 0 2758 5232 Size: 2758 2474 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 234 900 1785 5232 Size: 234 666 885 3447 Fragments arranged by size: 3447 885 666 234 FspI TGC'GCA Cuts at: 0 2227 2323 4993 5232 Size: 2227 96 2670 239 Fragments arranged by size: 2670 2227 239 96 HaeII r_GCGC'y Cuts at: 0 1801 2103 3616 4366 5232 Size: 1801 302 1513 750 866 Fragments arranged by size: 1801 1513 866 750 302 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 346 378 1057 1089 1526 1558 1667 1699 Size: 346 32 679 32 437 32 109 32 Cuts at: 1699 2764 2796 3919 3951 4372 4404 5043 5075 Size: 1065 32 1123 32 421 32 639 32 Cuts at: 5075 5232 Size: 157 Fragments arranged by size: 1123 1065 679 639 437 421 346 157 109 32 32 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 3612 4362 5232 Size: 3612 750 870 Fragments arranged by size: 3612 870 750 KpnI G_GTAC'C Cuts at: 0 474 5232 Size: 474 4758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 698 2286 3082 3776 4318 5232 Size: 698 1588 796 694 542 914 Fragments arranged by size: 1588 914 796 698 694 542 MscI TGG'CCA Cuts at: 0 458 1663 2464 4450 4738 5232 Size: 458 1205 801 1986 288 494 Fragments arranged by size: 1986 1205 801 494 458 288 MslI CAynn'nnrTG Cuts at: 0 526 776 2317 3655 5232 Size: 526 250 1541 1338 1577 Fragments arranged by size: 1577 1541 1338 526 250 MspA1I CmG'CkG Cuts at: 0 327 462 1948 2154 2544 2722 3577 4035 Size: 327 135 1486 206 390 178 855 458 Cuts at: 4035 4326 4978 5169 5232 Size: 291 652 191 63 Fragments arranged by size: 1486 855 652 458 390 327 291 206 191 178 135 63 NaeI GCC'GGC Cuts at: 0 4652 5232 Size: 4652 580 NarI GG'CG_CC Cuts at: 0 3613 4363 5232 Size: 3613 750 869 Fragments arranged by size: 3613 869 750 NcoI C'CATG_G Cuts at: 0 343 1644 2424 5232 Size: 343 1301 780 2808 Fragments arranged by size: 2808 1301 780 343 NdeI CA'TA_TG Cuts at: 0 840 5232 Size: 840 4392 NgoMIV G'CCGG_C Cuts at: 0 4650 5232 Size: 4650 582 NspI r_CATG'y Cuts at: 0 579 3162 3384 5232 Size: 579 2583 222 1848 Fragments arranged by size: 2583 1848 579 222 PflMI CCAn_nnn'nTGG Cuts at: 0 330 1195 1608 1659 3135 5232 Size: 330 865 413 51 1476 2097 Fragments arranged by size: 2097 1476 865 413 330 51 PfoI T'CCnGG_A Cuts at: 0 128 593 956 1253 3350 3467 4157 5201 Size: 128 465 363 297 2097 117 690 1044 Cuts at: 5201 5232 Size: 31 Fragments arranged by size: 2097 1044 690 465 363 297 128 117 31 PmlI CAC'GTG Cuts at: 0 2414 2604 5232 Size: 2414 190 2628 Fragments arranged by size: 2628 2414 190 PpuMI rG'GwC_Cy Cuts at: 0 102 632 1571 2292 2750 3252 3354 4137 Size: 102 530 939 721 458 502 102 783 Cuts at: 4137 4185 5232 Size: 48 1047 Fragments arranged by size: 1047 939 783 721 530 502 458 102 102 48 PshAI GACnn'nnGTC Cuts at: 0 1582 3359 5232 Size: 1582 1777 1873 Fragments arranged by size: 1873 1777 1582 PspOMI G'GGCC_C Cuts at: 0 1142 4555 5232 Size: 1142 3413 677 Fragments arranged by size: 3413 1142 677 PspXI vC'TCGA_Gb Cuts at: 0 2744 3020 5083 5232 Size: 2744 276 2063 149 Fragments arranged by size: 2744 2063 276 149 PstI C_TGCA'G Cuts at: 0 56 1026 1160 1334 1350 1833 2285 2795 Size: 56 970 134 174 16 483 452 510 Cuts at: 2795 3302 4034 4076 4307 4325 4820 5232 Size: 507 732 42 231 18 495 412 Fragments arranged by size: 970 732 510 507 495 483 452 412 231 174 134 56 42 18 16 PvuII CAG'CTG Cuts at: 0 327 2544 3577 4035 4326 5232 Size: 327 2217 1033 458 291 906 Fragments arranged by size: 2217 1033 906 458 327 291 SacI G_AGCT'C Cuts at: 0 1070 1718 5232 Size: 1070 648 3514 Fragments arranged by size: 3514 1070 648 SanDI GG'GwC_CC Cuts at: 0 102 5232 Size: 102 5130 SapI GCTCTTCn'nnn_ Cuts at: 0 277 403 2227 5232 Size: 277 126 1824 3005 Fragments arranged by size: 3005 1824 277 126 SbfI CC_TGCA'GG Cuts at: 0 2285 5232 Size: 2285 2947 SfcI C'TryA_G Cuts at: 0 52 1022 1156 1330 1346 1829 2281 2791 Size: 52 970 134 174 16 483 452 510 Cuts at: 2791 3298 4030 4072 4189 4303 4321 4816 4833 Size: 507 732 42 117 114 18 495 17 Cuts at: 4833 5232 Size: 399 Fragments arranged by size: 970 732 510 507 495 483 452 399 174 134 117 114 52 42 18 17 16 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2255 5232 Size: 2255 2977 SfoI GGC'GCC Cuts at: 0 3614 4364 5232 Size: 3614 750 868 Fragments arranged by size: 3614 868 750 SmaI CCC'GGG Cuts at: 0 1410 4597 5232 Size: 1410 3187 635 Fragments arranged by size: 3187 1410 635 SmlI C'TyrA_G Cuts at: 0 2744 3009 3020 5083 5232 Size: 2744 265 11 2063 149 Fragments arranged by size: 2744 2063 265 149 11 SphI G_CATG'C Cuts at: 0 3384 5232 Size: 3384 1848 SspI AAT'ATT Cuts at: 0 2381 5047 5232 Size: 2381 2666 185 Fragments arranged by size: 2666 2381 185 StuI AGG'CCT Cuts at: 0 1162 2453 5232 Size: 1162 1291 2779 Fragments arranged by size: 2779 1291 1162 StyI C'CwwG_G Cuts at: 0 343 1103 1163 1644 1808 2009 2424 2783 Size: 343 760 60 481 164 201 415 359 Cuts at: 2783 2849 2870 3122 3608 3885 4082 4280 4367 Size: 66 21 252 486 277 197 198 87 Cuts at: 4367 4961 5232 Size: 594 271 Fragments arranged by size: 760 594 486 481 415 359 343 277 271 252 201 198 197 164 87 66 60 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 700 1572 1670 3553 4564 4572 4957 5232 Size: 700 872 98 1883 1011 8 385 275 Fragments arranged by size: 1883 1011 872 700 385 275 98 8 TatI w'GTAC_w Cuts at: 0 572 3683 4223 5232 Size: 572 3111 540 1009 Fragments arranged by size: 3111 1009 572 540 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 137 169 2378 2410 2608 2640 2762 2794 Size: 137 32 2209 32 198 32 122 32 Cuts at: 2794 3096 3128 3720 3752 5232 Size: 302 32 592 32 1480 Fragments arranged by size: 2209 1480 592 302 198 137 122 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 4582 5232 Size: 4582 650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 525 1380 3048 3615 5232 Size: 525 855 1668 567 1617 Fragments arranged by size: 1668 1617 855 567 525 XhoI C'TCGA_G Cuts at: 0 2744 3020 5083 5232 Size: 2744 276 2063 149 Fragments arranged by size: 2744 2063 276 149 XmaI C'CCGG_G Cuts at: 0 1408 4595 5232 Size: 1408 3187 637 Fragments arranged by size: 3187 1408 637 XmnI GAAnn'nnTTC Cuts at: 0 880 4012 5232 Size: 880 3132 1220 Fragments arranged by size: 3132 1220 880 ZraI GAC'GTC Cuts at: 0 4461 5232 Size: 4461 771 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AflIII AhdI AloI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI SfcI SfiI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI AclI AflII AgeI AleI AlfI AscI AsiSI AvrII BaeI BciVI BfrBI BmtI BsaBI BsiWI BsmBI BspEI BssHII BstEII BstZ17I ClaI CspCI DraI EcoRV FseI FspAI HincII HindIII HpaI MfeI MluI NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PsiI PsrI PvuI RsrII SacII SalI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI