Restriction map for
pF1KSDB1510
(Linear) (Six-base) MAPSORT of: KIBB1510.seq Check: 1097 from: 1 to: 1743
KIBB1510.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AccI GT'mk_AC
Cuts at: 0 878 1743
Size: 878 865
Acc65I G'GTAC_C
Cuts at: 0 589 1743
Size: 589 1154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1182 1576 1585 1743
Size: 935 247 394 9 158
Fragments arranged by size:
935 394 247 158 9
AfeI AGC'GCT
Cuts at: 0 47 1743
Size: 47 1696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 583 1201 1743
Size: 583 618 542
Fragments arranged by size:
618 583 542
AlwNI CAG_nnn'CTG
Cuts at: 0 18 543 720 1038 1205 1743
Size: 18 525 177 318 167 538
Fragments arranged by size:
538 525 318 177 167 18
ApaI G_GGCC'C
Cuts at: 0 106 283 633 873 1743
Size: 106 177 350 240 870
Fragments arranged by size:
870 350 240 177 106
ApaLI G'TGCA_C
Cuts at: 0 596 1743
Size: 596 1147
AvaI C'yCGr_G
Cuts at: 0 181 1423 1499 1743
Size: 181 1242 76 244
Fragments arranged by size:
1242 244 181 76
BanI G'GyrC_C
Cuts at: 0 589 1743
Size: 589 1154
BanII G_rGCy'C
Cuts at: 0 106 182 188 283 624 633 873 1743
Size: 106 76 6 95 341 9 240 870
Fragments arranged by size:
870 341 240 106 95 76 9 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 22 56 423 433 457 467 1743
Size: 22 34 367 10 24 10 1276
Fragments arranged by size:
1276 367 34 24 22 10 10
BglI GCCn_nnn'nGGC
Cuts at: 0 229 287 1743
Size: 229 58 1456
Fragments arranged by size:
1456 229 58
BlpI GC'TnA_GC
Cuts at: 0 1406 1743
Size: 1406 337
Bme1580I G_kGCm'C
Cuts at: 0 106 283 600 633 873 1464 1743
Size: 106 177 317 33 240 591 279
Fragments arranged by size:
591 317 279 240 177 106 33
BmgBI CAC'GTC
Cuts at: 0 1443 1743
Size: 1443 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 565 669 1421 1453 1705 1743
Size: 478 87 104 752 32 252 38
Fragments arranged by size:
752 478 252 104 87 38 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 369 1076 1482 1743
Size: 369 707 406 261
Fragments arranged by size:
707 406 369 261
BsaAI yAC'GTr
Cuts at: 0 1212 1743
Size: 1212 531
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 1582 1705 1708 1743
Size: 475 1107 123 3 35
Fragments arranged by size:
1107 475 123 35 3
BseYI C'CCAG_C
Cuts at: 0 18 36 140 321 726 748 1000 1743
Size: 18 18 104 181 405 22 252 743
Fragments arranged by size:
743 405 252 181 104 22 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1743
Size: 1400 343
BsiEI CG_ry'CG
Cuts at: 0 78 100 291 1607 1743
Size: 78 22 191 1316 136
Fragments arranged by size:
1316 191 136 78 22
BsiHKAI G_wGCw'C
Cuts at: 0 562 600 624 1743
Size: 562 38 24 1119
Fragments arranged by size:
1119 562 38 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 202 429 1743
Size: 202 227 1314
Fragments arranged by size:
1314 227 202
Bsp1286I G_dGCh'C
Cuts at: 0 106 182 188 283 562 600 624 633
Size: 106 76 6 95 279 38 24 9
Cuts at: 633 873 1464 1743
Size: 240 591 279
Fragments arranged by size:
591 279 279 240 106 95 76 38
24 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 667 1422 1743
Size: 667 755 321
Fragments arranged by size:
755 667 321
BsrDI GCAATG_nn'
Cuts at: 0 529 1743
Size: 529 1214
BsrFI r'CCGG_y
Cuts at: 0 353 1743
Size: 353 1390
BssSI C'ACGA_G
Cuts at: 0 432 1743
Size: 432 1311
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1617 1743
Size: 1617 126
BstYI r'GATC_y
Cuts at: 0 380 1743
Size: 380 1363
BtgI C'CryG_G
Cuts at: 0 24 123 927 1256 1743
Size: 24 99 804 329 487
Fragments arranged by size:
804 487 329 99 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1056 1308 1743
Size: 1056 252 435
Fragments arranged by size:
1056 435 252
BtsI GCAGTG_nn'
Cuts at: 0 504 1286 1319 1417 1462 1743
Size: 504 782 33 98 45 281
Fragments arranged by size:
782 504 281 98 45 33
EaeI y'GGCC_r
Cuts at: 0 75 97 221 288 647 930 1362 1604
Size: 75 22 124 67 359 283 432 242
Cuts at: 1604 1619 1743
Size: 15 124
Fragments arranged by size:
432 359 283 242 124 124 75 67
22 15
EagI C'GGCC_G
Cuts at: 0 75 97 288 1604 1743
Size: 75 22 191 1316 139
Fragments arranged by size:
1316 191 139 75 22
EarI CTCTTCn'nnn_
Cuts at: 0 852 1743
Size: 852 891
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 361 1238 1743
Size: 199 162 877 505
Fragments arranged by size:
877 505 199 162
EcoICRI GAG'CTC
Cuts at: 0 622 1743
Size: 622 1121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 565 669 935 1182 1421 1453 1576
Size: 478 87 104 266 247 239 32 123
Cuts at: 1576 1585 1705 1743
Size: 9 120 38
Fragments arranged by size:
478 266 247 239 123 120 104 87
38 32 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 1743
Size: 331 1412
EcoO109I rG'GnC_Cy
Cuts at: 0 280 415 629 630 790 869 870 1743
Size: 280 135 214 1 160 79 1 873
Fragments arranged by size:
873 280 214 160 135 79 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1686 1743
Size: 1686 57
HaeII r_GCGC'y
Cuts at: 0 19 49 1743
Size: 19 30 1694
Fragments arranged by size:
1694 30 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1075 1107 1743
Size: 1075 32 636
Fragments arranged by size:
1075 636 32
HincII GTy'rAC
Cuts at: 0 879 1743
Size: 879 864
KpnI G_GTAC'C
Cuts at: 0 593 1743
Size: 593 1150
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 1118 1620 1743
Size: 407 711 502 123
Fragments arranged by size:
711 502 407 123
MscI TGG'CCA
Cuts at: 0 649 1621 1743
Size: 649 972 122
Fragments arranged by size:
972 649 122
MslI CAynn'nnrTG
Cuts at: 0 655 1105 1743
Size: 655 450 638
Fragments arranged by size:
655 638 450
MspA1I CmG'CkG
Cuts at: 0 26 36 125 246 858 1035 1205 1743
Size: 26 10 89 121 612 177 170 538
Fragments arranged by size:
612 538 177 170 121 89 26 10
NaeI GCC'GGC
Cuts at: 0 355 1743
Size: 355 1388
NcoI C'CATG_G
Cuts at: 0 927 1743
Size: 927 816
NgoMIV G'CCGG_C
Cuts at: 0 353 1743
Size: 353 1390
PasI CC'CwG_GG
Cuts at: 0 1274 1743
Size: 1274 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 543 720 1743
Size: 219 324 177 1023
Fragments arranged by size:
1023 324 219 177
PpuMI rG'GwC_Cy
Cuts at: 0 790 1743
Size: 790 953
PspOMI G'GGCC_C
Cuts at: 0 102 279 629 869 1743
Size: 102 177 350 240 874
Fragments arranged by size:
874 350 240 177 102
PstI C_TGCA'G
Cuts at: 0 35 499 1034 1190 1743
Size: 35 464 535 156 553
Fragments arranged by size:
553 535 464 156 35
PvuII CAG'CTG
Cuts at: 0 36 1035 1205 1743
Size: 36 999 170 538
Fragments arranged by size:
999 538 170 36
SacI G_AGCT'C
Cuts at: 0 624 1743
Size: 624 1119
SacII CC_GC'GG
Cuts at: 0 27 126 1743
Size: 27 99 1617
Fragments arranged by size:
1617 99 27
SalI G'TCGA_C
Cuts at: 0 877 1743
Size: 877 866
SfcI C'TryA_G
Cuts at: 0 31 495 1030 1186 1290 1743
Size: 31 464 535 156 104 453
Fragments arranged by size:
535 464 453 156 104 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 287 1743
Size: 287 1456
StuI AGG'CCT
Cuts at: 0 920 1743
Size: 920 823
StyI C'CwwG_G
Cuts at: 0 644 743 927 1743
Size: 644 99 184 816
Fragments arranged by size:
816 644 184 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1014 1743
Size: 1014 729
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1248 1280 1743
Size: 1248 32 463
Fragments arranged by size:
1248 463 32
XmnI GAAnn'nnTTC
Cuts at: 0 1544 1743
Size: 1544 199
Enzymes that do cut:
AccI Acc65I AcuI AfeI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BcgI BglI BlpI Bme1580I BmgBI
BpmI BsaI BsaAI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI
BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI
PflMI PpuMI PspOMI PstI PvuII SacI SacII SalI
SfcI SfiI StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmrI
BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI MfeI MluI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI ZraI