Restriction map for
pF1KB1755
(Linear) (Six-base) MAPSORT of: KIBB1755.seq Check: 2441 from: 1 to: 4395
KIBB1755.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1040 4395
Size: 1040 3355
AatII G_ACGT'C
Cuts at: 0 184 833 1879 2102 4395
Size: 184 649 1046 223 2293
Fragments arranged by size:
2293 1046 649 223 184
Acc65I G'GTAC_C
Cuts at: 0 77 263 947 4395
Size: 77 186 684 3448
Fragments arranged by size:
3448 684 186 77
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 940 1008 2535 2637 3730 3733 4395
Size: 846 94 68 1527 102 1093 3 662
Fragments arranged by size:
1527 1093 846 662 102 94 68 3
AfeI AGC'GCT
Cuts at: 0 307 4395
Size: 307 4088
AflIII A'CryG_T
Cuts at: 0 2477 2699 2810 4395
Size: 2477 222 111 1585
Fragments arranged by size:
2477 1585 222 111
AgeI A'CCGG_T
Cuts at: 0 1305 1543 4395
Size: 1305 238 2852
Fragments arranged by size:
2852 1305 238
AhdI GACnn_n'nnGTC
Cuts at: 0 2967 4395
Size: 2967 1428
AleI CACnn'nnGTG
Cuts at: 0 1546 4395
Size: 1546 2849
AlwNI CAG_nnn'CTG
Cuts at: 0 209 273 4395
Size: 209 64 4122
Fragments arranged by size:
4122 209 64
ApaI G_GGCC'C
Cuts at: 0 1146 4395
Size: 1146 3249
ApaLI G'TGCA_C
Cuts at: 0 894 1151 1549 2011 4395
Size: 894 257 398 462 2384
Fragments arranged by size:
2384 894 462 398 257
ApoI r'AATT_y
Cuts at: 0 2082 2203 3367 3985 4395
Size: 2082 121 1164 618 410
Fragments arranged by size:
2082 1164 618 410 121
AseI AT'TA_AT
Cuts at: 0 3152 4395
Size: 3152 1243
AvaI C'yCGr_G
Cuts at: 0 1076 1701 3001 4236 4395
Size: 1076 625 1300 1235 159
Fragments arranged by size:
1300 1235 1076 625 159
BanI G'GyrC_C
Cuts at: 0 77 263 947 2734 4395
Size: 77 186 684 1787 1661
Fragments arranged by size:
1787 1661 684 186 77
BanII G_rGCy'C
Cuts at: 0 459 1146 1237 1973 2387 2438 3002 3554
Size: 459 687 91 736 414 51 564 552
Cuts at: 3554 4395
Size: 841
Fragments arranged by size:
841 736 687 564 552 459 414 91
51
BbsI GAAGACnn'nnnn_
Cuts at: 0 149 2643 3293 4084 4395
Size: 149 2494 650 791 311
Fragments arranged by size:
2494 791 650 311 149
BbvCI CC'TCA_GC
Cuts at: 0 313 1585 4380 4395
Size: 313 1272 2795 15
Fragments arranged by size:
2795 1272 313 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 774 808 3428 3462 4020 4054 4395
Size: 774 34 2620 34 558 34 341
Fragments arranged by size:
2620 774 558 341 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1987 4395
Size: 1987 2408
BfrBI ATG'CAT
Cuts at: 0 3325 4395
Size: 3325 1070
BglI GCCn_nnn'nGGC
Cuts at: 0 4384 4395
Size: 4384 11
BglII A'GATC_T
Cuts at: 0 3809 4395
Size: 3809 586
BlpI GC'TnA_GC
Cuts at: 0 3113 4159 4395
Size: 3113 1046 236
Fragments arranged by size:
3113 1046 236
Bme1580I G_kGCm'C
Cuts at: 0 898 1146 1155 1553 2015 4395
Size: 898 248 9 398 462 2380
Fragments arranged by size:
2380 898 462 398 248 9
BmgBI CAC'GTC
Cuts at: 0 960 2480 2702 4056 4290 4395
Size: 960 1520 222 1354 234 105
Fragments arranged by size:
1520 1354 960 234 222 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 2390 3420 4395
Size: 2390 1030 975
Fragments arranged by size:
2390 1030 975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4188 4395
Size: 4188 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 661 1389 2588 2958 3091 3553 4395
Size: 156 505 728 1199 370 133 462 842
Fragments arranged by size:
1199 842 728 505 462 370 156 133
Bpu10I CC'TnA_GC
Cuts at: 0 313 1585 1777 1904 4380 4395
Size: 313 1272 192 127 2476 15
Fragments arranged by size:
2476 1272 313 192 127 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 874 3475 4395
Size: 162 712 2601 920
Fragments arranged by size:
2601 920 712 162
BsaI GGTCTCn'nnnn_
Cuts at: 0 1416 2625 2721 4395
Size: 1416 1209 96 1674
Fragments arranged by size:
1674 1416 1209 96
BsaAI yAC'GTr
Cuts at: 0 1935 4395
Size: 1935 2460
BsaHI Gr'CG_yC
Cuts at: 0 181 830 910 1085 1178 1520 1876 2099
Size: 181 649 80 175 93 342 356 223
Cuts at: 2099 4395
Size: 2296
Fragments arranged by size:
2296 649 356 342 223 181 175 93
80
BsaWI w'CCGG_w
Cuts at: 0 1305 1543 2913 3955 4395
Size: 1305 238 1370 1042 440
Fragments arranged by size:
1370 1305 1042 440 238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 1783 1813 2158 2188 4395
Size: 490 30 1263 30 345 30 2207
Fragments arranged by size:
2207 1263 490 345 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 349 1927 2146 2572 2857 3544 4395
Size: 256 93 1578 219 426 285 687 851
Fragments arranged by size:
1578 851 687 426 285 256 219 93
BseYI C'CCAG_C
Cuts at: 0 1589 1923 2290 4343 4395
Size: 1589 334 367 2053 52
Fragments arranged by size:
2053 1589 367 334 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 949 1018 1309 1412 1909 2131 3289
Size: 574 375 69 291 103 497 222 1158
Cuts at: 3289 3708 4080 4395
Size: 419 372 315
Fragments arranged by size:
1158 574 497 419 375 372 315 291
222 103 69
BsiEI CG_ry'CG
Cuts at: 0 154 4395
Size: 154 4241
BsiHKAI G_wGCw'C
Cuts at: 0 229 898 1155 1553 2015 2405 3002 3119
Size: 229 669 257 398 462 390 597 117
Cuts at: 3119 3554 4169 4395
Size: 435 615 226
Fragments arranged by size:
669 615 597 462 435 398 390 257
229 226 117
BsmI GAATG_Cn'
Cuts at: 0 3325 4395
Size: 3325 1070
BsmBI CGTCTCn'nnnn_
Cuts at: 0 746 871 2819 4395
Size: 746 125 1948 1576
Fragments arranged by size:
1948 1576 746 125
Bsp1286I G_dGCh'C
Cuts at: 0 229 459 898 1146 1155 1237 1553 1973
Size: 229 230 439 248 9 82 316 420
Cuts at: 1973 2015 2387 2405 2438 3002 3119 3554 4169
Size: 42 372 18 33 564 117 435 615
Cuts at: 4169 4395
Size: 226
Fragments arranged by size:
615 564 439 435 420 372 316 248
230 229 226 117 82 42 33 18
9
BspEI T'CCGG_A
Cuts at: 0 2913 3955 4395
Size: 2913 1042 440
Fragments arranged by size:
2913 1042 440
BspHI T'CATG_A
Cuts at: 0 3276 3755 4395
Size: 3276 479 640
Fragments arranged by size:
3276 640 479
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 251 1040 1928 2636 4022 4102 4395
Size: 89 162 789 888 708 1386 80 293
Fragments arranged by size:
1386 888 789 708 293 162 89 80
BsrBI CCG'CTC
Cuts at: 0 444 1623 1658 4395
Size: 444 1179 35 2737
Fragments arranged by size:
2737 1179 444 35
BsrDI GCAATG_nn'
Cuts at: 0 1302 3030 3683 4395
Size: 1302 1728 653 712
Fragments arranged by size:
1728 1302 712 653
BsrFI r'CCGG_y
Cuts at: 0 1305 1543 1766 2371 4395
Size: 1305 238 223 605 2024
Fragments arranged by size:
2024 1305 605 238 223
BsrGI T'GTAC_A
Cuts at: 0 1055 4304 4395
Size: 1055 3249 91
Fragments arranged by size:
3249 1055 91
BssHII G'CGCG_C
Cuts at: 0 936 4395
Size: 936 3459
BssSI C'ACGA_G
Cuts at: 0 2272 4395
Size: 2272 2123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2671 4395
Size: 2671 1724
BstEII G'GTnAC_C
Cuts at: 0 855 4059 4395
Size: 855 3204 336
Fragments arranged by size:
3204 855 336
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 2143 4395
Size: 549 1594 2252
Fragments arranged by size:
2252 1594 549
BstYI r'GATC_y
Cuts at: 0 464 1696 2391 3809 4322 4395
Size: 464 1232 695 1418 513 73
Fragments arranged by size:
1418 1232 695 513 464 73
BtgI C'CryG_G
Cuts at: 0 548 702 1157 1608 1646 2024 2879 3582
Size: 548 154 455 451 38 378 855 703
Cuts at: 3582 4395
Size: 813
Fragments arranged by size:
855 813 703 548 455 451 378 154
38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 968 4395
Size: 968 3427
BtsI GCAGTG_nn'
Cuts at: 0 562 668 692 1174 1297 1382 1897 3520
Size: 562 106 24 482 123 85 515 1623
Cuts at: 3520 4395
Size: 875
Fragments arranged by size:
1623 875 562 515 482 123 106 85
24
ClaI AT'CG_AT
Cuts at: 0 805 3234 4395
Size: 805 2429 1161
Fragments arranged by size:
2429 1161 805
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2663 2698 4395
Size: 2663 35 1697
Fragments arranged by size:
2663 1697 35
DraI TTT'AAA
Cuts at: 0 2615 2868 4395
Size: 2615 253 1527
Fragments arranged by size:
2615 1527 253
DraIII CAC_nnn'GTG
Cuts at: 0 2828 4395
Size: 2828 1567
DrdI GACnn_nn'nnGTC
Cuts at: 0 1132 2811 3163 4395
Size: 1132 1679 352 1232
Fragments arranged by size:
1679 1232 1132 352
EaeI y'GGCC_r
Cuts at: 0 278 689 1949 2169 2369 4395
Size: 278 411 1260 220 200 2026
Fragments arranged by size:
2026 1260 411 278 220 200
EarI CTCTTCn'nnn_
Cuts at: 0 25 434 2990 3338 3842 4395
Size: 25 409 2556 348 504 553
Fragments arranged by size:
2556 553 504 409 348 25
EcoICRI GAG'CTC
Cuts at: 0 3000 3552 4395
Size: 3000 552 843
Fragments arranged by size:
3000 843 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 661 846 940 1008 1389 2535 2588
Size: 156 505 185 94 68 381 1146 53
Cuts at: 2588 2637 2958 3091 3553 3730 3733 4395
Size: 49 321 133 462 177 3 662
Fragments arranged by size:
1146 662 505 462 381 321 185 177
156 133 94 68 53 49 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 864 1036 4395
Size: 864 172 3359
Fragments arranged by size:
3359 864 172
EcoO109I rG'GnC_Cy
Cuts at: 0 1050 1142 1143 1534 1617 1917 2196 2502
Size: 1050 92 1 391 83 300 279 306
Cuts at: 2502 3257 3385 4395
Size: 755 128 1010
Fragments arranged by size:
1050 1010 755 391 306 300 279 128
92 83 1
EcoRI G'AATT_C
Cuts at: 0 3985 4395
Size: 3985 410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1011 2919 3317 3457 3618 4395
Size: 1011 1908 398 140 161 777
Fragments arranged by size:
1908 1011 777 398 161 140
FspI TGC'GCA
Cuts at: 0 1066 4395
Size: 1066 3329
HaeII r_GCGC'y
Cuts at: 0 309 1103 4395
Size: 309 794 3292
Fragments arranged by size:
3292 794 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 182 214 490 522 870 902 1783 1815
Size: 182 32 276 32 348 32 881 32
Cuts at: 1815 2158 2190 2561 2593 2953 2985 3106 3138
Size: 343 32 371 32 360 32 121 32
Cuts at: 3138 4071 4103 4395
Size: 933 32 292
Fragments arranged by size:
933 881 371 360 348 343 292 276
182 121 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1677 2364 4395
Size: 1677 687 2031
Fragments arranged by size:
2031 1677 687
HindIII A'AGCT_T
Cuts at: 0 3962 4395
Size: 3962 433
KpnI G_GTAC'C
Cuts at: 0 81 267 951 4395
Size: 81 186 684 3444
Fragments arranged by size:
3444 684 186 81
MluI A'CGCG_T
Cuts at: 0 2810 4395
Size: 2810 1585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2684 4395
Size: 2684 1711
MscI TGG'CCA
Cuts at: 0 691 4395
Size: 691 3704
MslI CAynn'nnrTG
Cuts at: 0 547 904 1546 2107 2141 3413 3800 4395
Size: 547 357 642 561 34 1272 387 595
Fragments arranged by size:
1272 642 595 561 547 387 357 34
MspA1I CmG'CkG
Cuts at: 0 150 1131 1589 1664 1690 4384 4395
Size: 150 981 458 75 26 2694 11
Fragments arranged by size:
2694 981 458 150 75 26 11
NaeI GCC'GGC
Cuts at: 0 2373 4395
Size: 2373 2022
NcoI C'CATG_G
Cuts at: 0 1157 4395
Size: 1157 3238
NdeI CA'TA_TG
Cuts at: 0 3827 4395
Size: 3827 568
NgoMIV G'CCGG_C
Cuts at: 0 2371 4395
Size: 2371 2024
NsiI A_TGCA'T
Cuts at: 0 3327 4395
Size: 3327 1068
PasI CC'CwG_GG
Cuts at: 0 186 4395
Size: 186 4209
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 840 1964 4395
Size: 273 567 1124 2431
Fragments arranged by size:
2431 1124 567 273
PfoI T'CCnGG_A
Cuts at: 0 4325 4395
Size: 4325 70
PpuMI rG'GwC_Cy
Cuts at: 0 1617 1917 2196 3257 4395
Size: 1617 300 279 1061 1138
Fragments arranged by size:
1617 1138 1061 300 279
PshAI GACnn'nnGTC
Cuts at: 0 711 1828 2647 4395
Size: 711 1117 819 1748
Fragments arranged by size:
1748 1117 819 711
PspOMI G'GGCC_C
Cuts at: 0 1142 4395
Size: 1142 3253
PspXI vC'TCGA_Gb
Cuts at: 0 3001 4395
Size: 3001 1394
PstI C_TGCA'G
Cuts at: 0 205 2633 3866 4395
Size: 205 2428 1233 529
Fragments arranged by size:
2428 1233 529 205
PvuII CAG'CTG
Cuts at: 0 1589 1664 1690 4395
Size: 1589 75 26 2705
Fragments arranged by size:
2705 1589 75 26
SacI G_AGCT'C
Cuts at: 0 3002 3554 4395
Size: 3002 552 841
Fragments arranged by size:
3002 841 552
SapI GCTCTTCn'nnn_
Cuts at: 0 434 2990 4395
Size: 434 2556 1405
Fragments arranged by size:
2556 1405 434
ScaI AGT'ACT
Cuts at: 0 3403 4395
Size: 3403 992
SexAI A'CCwGG_T
Cuts at: 0 2824 4395
Size: 2824 1571
SfcI C'TryA_G
Cuts at: 0 201 2629 3862 4395
Size: 201 2428 1233 533
Fragments arranged by size:
2428 1233 533 201
SgrAI Cr'CCGG_yG
Cuts at: 0 1305 1543 4395
Size: 1305 238 2852
Fragments arranged by size:
2852 1305 238
SmlI C'TyrA_G
Cuts at: 0 141 889 3001 3490 4395
Size: 141 748 2112 489 905
Fragments arranged by size:
2112 905 748 489 141
SspI AAT'ATT
Cuts at: 0 66 4395
Size: 66 4329
StuI AGG'CCT
Cuts at: 0 1012 2419 4395
Size: 1012 1407 1976
Fragments arranged by size:
1976 1407 1012
StyI C'CwwG_G
Cuts at: 0 1157 1439 2483 2738 4395
Size: 1157 282 1044 255 1657
Fragments arranged by size:
1657 1157 1044 282 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 1170 1274 1275 1614 1913 2304 4395
Size: 133 1037 104 1 339 299 391 2091
Fragments arranged by size:
2091 1037 391 339 299 133 104 1
TatI w'GTAC_w
Cuts at: 0 605 1055 1628 2036 2510 2900 3401 4244
Size: 605 450 573 408 474 390 501 843
Cuts at: 4244 4304 4395
Size: 60 91
Fragments arranged by size:
843 605 573 501 474 450 408 390
91 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1153 1185 2111 2143 2528 2560 4395
Size: 1153 32 926 32 385 32 1835
Fragments arranged by size:
1835 1153 926 385 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1192 4395
Size: 1192 3203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 726 1878 2982 4395
Size: 726 1152 1104 1413
Fragments arranged by size:
1413 1152 1104 726
XhoI C'TCGA_G
Cuts at: 0 3001 4395
Size: 3001 1394
XmnI GAAnn'nnTTC
Cuts at: 0 991 4395
Size: 991 3404
ZraI GAC'GTC
Cuts at: 0 182 831 1877 2100 4395
Size: 182 649 1046 223 2295
Fragments arranged by size:
2295 1046 649 223 182
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AvaI BanI
BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KpnI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NsiI PasI PflMI PfoI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SapI ScaI SexAI SfcI
SgrAI SmlI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AlfI AloI AscI AsiSI AvrII
BaeI BamHI BbeI BclI BmtI BsaBI BsiWI BstBI
BstZ17I Bsu36I EagI EciI EcoRV FseI FspAI HpaI
KasI MfeI NarI NheI NotI NruI NspI PacI
PciI PmeI PmlI PpiI PsiI PsrI PvuI RsrII
SacII SalI SanDI SbfI SfiI SfoI SgfI SmaI
SnaBI SpeI SphI SrfI SwaI XbaI XmaI