Restriction map for
pF1KB1986
(Linear) (Six-base) MAPSORT of: KIBB1986.seq Check: 9200 from: 1 to: 3309
KIBB1986.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AccI GT'mk_AC
Cuts at: 0 1244 3309
Size: 1244 2065
AclI AA'CG_TT
Cuts at: 0 2219 3309
Size: 2219 1090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1943 2512 2726 3309
Size: 1288 655 569 214 583
Fragments arranged by size:
1288 655 583 569 214
AflII C'TTAA_G
Cuts at: 0 2320 3309
Size: 2320 989
AflIII A'CryG_T
Cuts at: 0 2718 3309
Size: 2718 591
AleI CACnn'nnGTG
Cuts at: 0 1400 3309
Size: 1400 1909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2675 3309
Size: 2675 634
AlwNI CAG_nnn'CTG
Cuts at: 0 2169 3309
Size: 2169 1140
ApoI r'AATT_y
Cuts at: 0 297 1075 1540 1661 2049 2141 2698 2750
Size: 297 778 465 121 388 92 557 52
Cuts at: 2750 2815 3309
Size: 65 494
Fragments arranged by size:
778 557 494 465 388 297 121 92
65 52
AvaI C'yCGr_G
Cuts at: 0 10 2882 3309
Size: 10 2872 427
Fragments arranged by size:
2872 427 10
BamHI G'GATC_C
Cuts at: 0 1410 2148 3309
Size: 1410 738 1161
Fragments arranged by size:
1410 1161 738
BanII G_rGCy'C
Cuts at: 0 1746 2377 3309
Size: 1746 631 932
Fragments arranged by size:
1746 932 631
BbsI GAAGACnn'nnnn_
Cuts at: 0 497 2012 2110 2213 2246 3079 3309
Size: 497 1515 98 103 33 833 230
Fragments arranged by size:
1515 833 497 230 103 98 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 1781 2419 3309
Size: 1781 638 890
Fragments arranged by size:
1781 890 638
BglI GCCn_nnn'nGGC
Cuts at: 0 2041 3309
Size: 2041 1268
BglII A'GATC_T
Cuts at: 0 1110 2025 3309
Size: 1110 915 1284
Fragments arranged by size:
1284 1110 915
BlpI GC'TnA_GC
Cuts at: 0 269 3309
Size: 269 3040
BmrI ACTGGGnnnn_n'
Cuts at: 0 2117 2568 3309
Size: 2117 451 741
Fragments arranged by size:
2117 741 451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2248 3309
Size: 2248 1061
Bpu10I CC'TnA_GC
Cuts at: 0 2288 3309
Size: 2288 1021
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1547 1632 2547 2740 3309
Size: 224 1323 85 915 193 569
Fragments arranged by size:
1323 915 569 224 193 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 3224 3309
Size: 3224 85
BsaAI yAC'GTr
Cuts at: 0 2721 3309
Size: 2721 588
BsaHI Gr'CG_yC
Cuts at: 0 22 3309
Size: 22 3287
BsaWI w'CCGG_w
Cuts at: 0 600 3309
Size: 600 2709
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1736 1745 1754 1820 1850 1865 3309
Size: 1736 9 9 66 30 15 1444
Fragments arranged by size:
1736 1444 66 30 15 9 9
BseYI C'CCAG_C
Cuts at: 0 608 3309
Size: 608 2701
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3239 3309
Size: 3239 70
BsiEI CG_ry'CG
Cuts at: 0 535 3309
Size: 535 2774
BsiHKAI G_wGCw'C
Cuts at: 0 1746 2377 3309
Size: 1746 631 932
Fragments arranged by size:
1746 932 631
BsmI GAATG_Cn'
Cuts at: 0 499 3223 3309
Size: 499 2724 86
Fragments arranged by size:
2724 499 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1207 2404 3309
Size: 1207 1197 905
Fragments arranged by size:
1207 1197 905
Bsp1286I G_dGCh'C
Cuts at: 0 1746 2377 3309
Size: 1746 631 932
Fragments arranged by size:
1746 932 631
BspHI T'CATG_A
Cuts at: 0 522 3309
Size: 522 2787
BsrBI CCG'CTC
Cuts at: 0 31 3309
Size: 31 3278
BsrDI GCAATG_nn'
Cuts at: 0 1687 3309
Size: 1687 1622
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3091 3309
Size: 3091 218
BstBI TT'CG_AA
Cuts at: 0 1056 1626 3309
Size: 1056 570 1683
Fragments arranged by size:
1683 1056 570
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2181 3309
Size: 2181 1128
BstYI r'GATC_y
Cuts at: 0 321 1110 1410 2025 2148 3309
Size: 321 789 300 615 123 1161
Fragments arranged by size:
1161 789 615 321 300 123
Bsu36I CC'TnA_GG
Cuts at: 0 577 3309
Size: 577 2732
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 262 3309
Size: 262 3047
BtsI GCAGTG_nn'
Cuts at: 0 434 1274 1395 1407 3041 3309
Size: 434 840 121 12 1634 268
Fragments arranged by size:
1634 840 434 268 121 12
ClaI AT'CG_AT
Cuts at: 0 1445 3309
Size: 1445 1864
EaeI y'GGCC_r
Cuts at: 0 624 3309
Size: 624 2685
EarI CTCTTCn'nnn_
Cuts at: 0 98 356 875 1754 1930 2981 3051 3062
Size: 98 258 519 879 176 1051 70 11
Cuts at: 3062 3309
Size: 247
Fragments arranged by size:
1051 879 519 258 247 176 98 70
11
EcoICRI GAG'CTC
Cuts at: 0 1744 2375 3309
Size: 1744 631 934
Fragments arranged by size:
1744 934 631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1943 2248 2512 2726 3309
Size: 1288 655 305 264 214 583
Fragments arranged by size:
1288 655 583 305 264 214
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1865 3309
Size: 1865 1444
EcoO109I rG'GnC_Cy
Cuts at: 0 1669 1825 3309
Size: 1669 156 1484
Fragments arranged by size:
1669 1484 156
EcoRI G'AATT_C
Cuts at: 0 1075 1540 1661 3309
Size: 1075 465 121 1648
Fragments arranged by size:
1648 1075 465 121
EcoRV GAT'ATC
Cuts at: 0 2691 3309
Size: 2691 618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1588 2613 3309
Size: 1588 1025 696
Fragments arranged by size:
1588 1025 696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 1427 1459 2576 2608 2640 3177
Size: 274 32 1121 32 1117 32 32 537
Cuts at: 3177 3209 3309
Size: 32 100
Fragments arranged by size:
1121 1117 537 274 100 32 32 32
32 32
HincII GTy'rAC
Cuts at: 0 615 3309
Size: 615 2694
HindIII A'AGCT_T
Cuts at: 0 664 2505 3309
Size: 664 1841 804
Fragments arranged by size:
1841 804 664
HpaI GTT'AAC
Cuts at: 0 615 3309
Size: 615 2694
MfeI C'AATT_G
Cuts at: 0 809 956 1944 3309
Size: 809 147 988 1365
Fragments arranged by size:
1365 988 809 147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 855 2520 3309
Size: 432 423 1665 789
Fragments arranged by size:
1665 789 432 423
MscI TGG'CCA
Cuts at: 0 626 3309
Size: 626 2683
MslI CAynn'nnrTG
Cuts at: 0 1400 3275 3309
Size: 1400 1875 34
Fragments arranged by size:
1875 1400 34
MspA1I CmG'CkG
Cuts at: 0 608 2629 3309
Size: 608 2021 680
Fragments arranged by size:
2021 680 608
NdeI CA'TA_TG
Cuts at: 0 2202 3309
Size: 2202 1107
NruI TCG'CGA
Cuts at: 0 247 3309
Size: 247 3062
NspI r_CATG'y
Cuts at: 0 1011 2199 2247 3309
Size: 1011 1188 48 1062
Fragments arranged by size:
1188 1062 1011 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 1846 2580 3309
Size: 1846 734 729
Fragments arranged by size:
1846 734 729
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2545 2577 3309
Size: 2545 32 732
Fragments arranged by size:
2545 732 32
PpuMI rG'GwC_Cy
Cuts at: 0 1669 1825 3309
Size: 1669 156 1484
Fragments arranged by size:
1669 1484 156
PsiI TTA'TAA
Cuts at: 0 1018 1358 3309
Size: 1018 340 1951
Fragments arranged by size:
1951 1018 340
PstI C_TGCA'G
Cuts at: 0 267 1402 1515 1680 3309
Size: 267 1135 113 165 1629
Fragments arranged by size:
1629 1135 267 165 113
PvuI CG_AT'CG
Cuts at: 0 535 3309
Size: 535 2774
PvuII CAG'CTG
Cuts at: 0 608 3309
Size: 608 2701
SacI G_AGCT'C
Cuts at: 0 1746 2377 3309
Size: 1746 631 932
Fragments arranged by size:
1746 932 631
ScaI AGT'ACT
Cuts at: 0 1643 3309
Size: 1643 1666
SfcI C'TryA_G
Cuts at: 0 263 446 1398 1511 1676 3309
Size: 263 183 952 113 165 1633
Fragments arranged by size:
1633 952 263 183 165 113
SmaI CCC'GGG
Cuts at: 0 12 3309
Size: 12 3297
SmlI C'TyrA_G
Cuts at: 0 239 1562 1647 2320 2562 2755 3309
Size: 239 1323 85 673 242 193 554
Fragments arranged by size:
1323 673 554 242 239 193 85
SphI G_CATG'C
Cuts at: 0 1011 2199 3309
Size: 1011 1188 1110
Fragments arranged by size:
1188 1110 1011
SspI AAT'ATT
Cuts at: 0 693 1354 3309
Size: 693 661 1955
Fragments arranged by size:
1955 693 661
StyI C'CwwG_G
Cuts at: 0 1845 3250 3309
Size: 1845 1405 59
Fragments arranged by size:
1845 1405 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 185 3309
Size: 185 3124
TatI w'GTAC_w
Cuts at: 0 1641 3309
Size: 1641 1668
XbaI T'CTAG_A
Cuts at: 0 891 3198 3309
Size: 891 2307 111
Fragments arranged by size:
2307 891 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 634 3309
Size: 408 226 2675
Fragments arranged by size:
2675 408 226
XmaI C'CCGG_G
Cuts at: 0 10 3309
Size: 10 3299
XmnI GAAnn'nnTTC
Cuts at: 0 518 665 1075 1984 3309
Size: 518 147 410 909 1325
Fragments arranged by size:
1325 909 518 410 147
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AleI AlfI AlwNI
ApoI AvaI BamHI BanII BbsI BciVI BglI BglII
BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrBI BsrDI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI ClaI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NdeI NruI
NspI PflMI PpiI PpuMI PsiI PstI PvuI PvuII
SacI ScaI SfcI SmaI SmlI SphI SspI StyI
TaqII TatI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbvCI BcgI BclI BfrBI Bme1580I BmgBI BmtI BplI
BsaBI BsaXI BsiWI BspEI BspMI BsrFI BsrGI BssHII
BssSI BstEII BstZ17I BtgI CspCI DraI DraIII DrdI
EagI EciI FseI FspI FspAI HaeII KasI KpnI
MluI NaeI NarI NcoI NgoMIV NheI NotI NsiI
PacI PasI PciI PfoI PmeI PmlI PshAI PspOMI
PspXI PsrI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
StuI SwaI TstI Tth111I XhoI ZraI