Restriction map for
pF1KB2048
(Linear) (Six-base) MAPSORT of: KIBB2048.seq Check: 218 from: 1 to: 3051
KIBB2048.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 2822 3051
Size: 2822 229
Acc65I G'GTAC_C
Cuts at: 0 2831 3051
Size: 2831 220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 2062 2182 2980 3051
Size: 31 2031 120 798 71
Fragments arranged by size:
2031 798 120 71 31
AflIII A'CryG_T
Cuts at: 0 940 3051
Size: 940 2111
AlwNI CAG_nnn'CTG
Cuts at: 0 1236 1503 1561 3051
Size: 1236 267 58 1490
Fragments arranged by size:
1490 1236 267 58
ApoI r'AATT_y
Cuts at: 0 494 823 3051
Size: 494 329 2228
Fragments arranged by size:
2228 494 329
AvaI C'yCGr_G
Cuts at: 0 1452 1811 2685 3051
Size: 1452 359 874 366
Fragments arranged by size:
1452 874 366 359
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1503 1536 3051
Size: 1503 33 1515
Fragments arranged by size:
1515 1503 33
BamHI G'GATC_C
Cuts at: 0 2210 3051
Size: 2210 841
BanI G'GyrC_C
Cuts at: 0 797 1468 1537 2831 3051
Size: 797 671 69 1294 220
Fragments arranged by size:
1294 797 671 220 69
BanII G_rGCy'C
Cuts at: 0 1273 1459 3051
Size: 1273 186 1592
Fragments arranged by size:
1592 1273 186
BbeI G_GCGC'C
Cuts at: 0 1541 3051
Size: 1541 1510
BbsI GAAGACnn'nnnn_
Cuts at: 0 781 3051
Size: 781 2270
BbvCI CC'TCA_GC
Cuts at: 0 1804 3051
Size: 1804 1247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 306 340 2855 2889 3051
Size: 306 34 2515 34 162
Fragments arranged by size:
2515 306 162 34 34
BglII A'GATC_T
Cuts at: 0 2477 3051
Size: 2477 574
BlpI GC'TnA_GC
Cuts at: 0 682 1272 2152 3051
Size: 682 590 880 899
Fragments arranged by size:
899 880 682 590
Bme1580I G_kGCm'C
Cuts at: 0 800 1471 3051
Size: 800 671 1580
Fragments arranged by size:
1580 800 671
BmgBI CAC'GTC
Cuts at: 0 2238 3051
Size: 2238 813
BmrI ACTGGGnnnn_n'
Cuts at: 0 1421 2497 2896 3051
Size: 1421 1076 399 155
Fragments arranged by size:
1421 1076 399 155
BmtI G_CTAG'C
Cuts at: 0 439 3051
Size: 439 2612
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 3051
Size: 1287 1764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 122 130 315 1500 1843 1967 2065
Size: 110 12 8 185 1185 343 124 98
Cuts at: 2065 3051
Size: 986
Fragments arranged by size:
1185 986 343 185 124 110 98 12
8
Bpu10I CC'TnA_GC
Cuts at: 0 1096 1804 1862 2646 3051
Size: 1096 708 58 784 405
Fragments arranged by size:
1096 784 708 405 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1189 1912 1918 3051
Size: 557 632 723 6 1133
Fragments arranged by size:
1133 723 632 557 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 422 619 1808 2731 3051
Size: 422 197 1189 923 320
Fragments arranged by size:
1189 923 422 320 197
BsaAI yAC'GTr
Cuts at: 0 302 3051
Size: 302 2749
BsaHI Gr'CG_yC
Cuts at: 0 1538 2633 3051
Size: 1538 1095 418
Fragments arranged by size:
1538 1095 418
BsaWI w'CCGG_w
Cuts at: 0 380 800 2892 3051
Size: 380 420 2092 159
Fragments arranged by size:
2092 420 380 159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1614 1644 1999 2029 3051
Size: 1614 30 355 30 1022
Fragments arranged by size:
1614 1022 355 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1117 1120 2290 2806 3051
Size: 1117 3 1170 516 245
Fragments arranged by size:
1170 1117 516 245 3
BseYI C'CCAG_C
Cuts at: 0 313 1020 1166 3051
Size: 313 707 146 1885
Fragments arranged by size:
1885 707 313 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2009 2621 2749 3051
Size: 2009 612 128 302
Fragments arranged by size:
2009 612 302 128
BsiEI CG_ry'CG
Cuts at: 0 955 3051
Size: 955 2096
BsiHKAI G_wGCw'C
Cuts at: 0 1737 2647 3051
Size: 1737 910 404
Fragments arranged by size:
1737 910 404
BsmI GAATG_Cn'
Cuts at: 0 480 1057 1118 1429 1623 2071 3051
Size: 480 577 61 311 194 448 980
Fragments arranged by size:
980 577 480 448 311 194 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1957 2185 2302 3051
Size: 1957 228 117 749
Fragments arranged by size:
1957 749 228 117
Bsp1286I G_dGCh'C
Cuts at: 0 800 1273 1459 1471 1737 2647 3051
Size: 800 473 186 12 266 910 404
Fragments arranged by size:
910 800 473 404 266 186 12
BspEI T'CCGG_A
Cuts at: 0 380 3051
Size: 380 2671
BspHI T'CATG_A
Cuts at: 0 1154 3051
Size: 1154 1897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1593 3051
Size: 1593 1458
BsrBI CCG'CTC
Cuts at: 0 920 1713 3051
Size: 920 793 1338
Fragments arranged by size:
1338 920 793
BsrDI GCAATG_nn'
Cuts at: 0 2340 3051
Size: 2340 711
BsrGI T'GTAC_A
Cuts at: 0 2105 3051
Size: 2105 946
BssSI C'ACGA_G
Cuts at: 0 2797 3051
Size: 2797 254
BstEII G'GTnAC_C
Cuts at: 0 689 3051
Size: 689 2362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1266 3051
Size: 1266 1785
BstYI r'GATC_y
Cuts at: 0 2210 2477 3051
Size: 2210 267 574
Fragments arranged by size:
2210 574 267
Bsu36I CC'TnA_GG
Cuts at: 0 3040 3051
Size: 3040 11
BtgI C'CryG_G
Cuts at: 0 1424 3051
Size: 1424 1627
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 410 1856 3051
Size: 410 1446 1195
Fragments arranged by size:
1446 1195 410
BtsI GCAGTG_nn'
Cuts at: 0 364 743 1839 2216 3051
Size: 364 379 1096 377 835
Fragments arranged by size:
1096 835 379 377 364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2915 2950 3051
Size: 2915 35 101
Fragments arranged by size:
2915 101 35
DraIII CAC_nnn'GTG
Cuts at: 0 1732 3017 3051
Size: 1732 1285 34
Fragments arranged by size:
1732 1285 34
EaeI y'GGCC_r
Cuts at: 0 952 1368 3051
Size: 952 416 1683
Fragments arranged by size:
1683 952 416
EagI C'GGCC_G
Cuts at: 0 952 3051
Size: 952 2099
EarI CTCTTCn'nnn_
Cuts at: 0 1105 1240 1485 2423 2713 2941 3013 3051
Size: 1105 135 245 938 290 228 72 38
Fragments arranged by size:
1105 938 290 245 228 135 72 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 110 122 130 315 1500 1843 1967
Size: 31 79 12 8 185 1185 343 124
Cuts at: 1967 2062 2065 2182 2980 3051
Size: 95 3 117 798 71
Fragments arranged by size:
1185 798 343 185 124 117 95 79
71 31 12 8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 1099 3051
Size: 148 951 1952
Fragments arranged by size:
1952 951 148
EcoO109I rG'GnC_Cy
Cuts at: 0 546 739 1808 3051
Size: 546 193 1069 1243
Fragments arranged by size:
1243 1069 546 193
EcoRV GAT'ATC
Cuts at: 0 1320 3051
Size: 1320 1731
HaeII r_GCGC'y
Cuts at: 0 1541 2368 3006 3051
Size: 1541 827 638 45
Fragments arranged by size:
1541 827 638 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 186 218 3051
Size: 186 32 2833
Fragments arranged by size:
2833 186 32
HincII GTy'rAC
Cuts at: 0 27 539 1299 1658 2927 3051
Size: 27 512 760 359 1269 124
Fragments arranged by size:
1269 760 512 359 124 27
HindIII A'AGCT_T
Cuts at: 0 340 3051
Size: 340 2711
HpaI GTT'AAC
Cuts at: 0 1658 3051
Size: 1658 1393
KasI G'GCGC_C
Cuts at: 0 1537 3051
Size: 1537 1514
KpnI G_GTAC'C
Cuts at: 0 2835 3051
Size: 2835 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1783 2705 3051
Size: 602 1181 922 346
Fragments arranged by size:
1181 922 602 346
MslI CAynn'nnrTG
Cuts at: 0 1960 2113 3051
Size: 1960 153 938
Fragments arranged by size:
1960 938 153
MspA1I CmG'CkG
Cuts at: 0 686 957 1236 1404 1765 1987 2231 2374
Size: 686 271 279 168 361 222 244 143
Cuts at: 2374 3051
Size: 677
Fragments arranged by size:
686 677 361 279 271 244 222 168
143
NarI GG'CG_CC
Cuts at: 0 1538 3051
Size: 1538 1513
NheI G'CTAG_C
Cuts at: 0 435 3051
Size: 435 2616
NspI r_CATG'y
Cuts at: 0 215 944 3051
Size: 215 729 2107
Fragments arranged by size:
2107 729 215
PciI A'CATG_T
Cuts at: 0 940 3051
Size: 940 2111
PfoI T'CCnGG_A
Cuts at: 0 146 1003 1820 3051
Size: 146 857 817 1231
Fragments arranged by size:
1231 857 817 146
PpuMI rG'GwC_Cy
Cuts at: 0 546 3051
Size: 546 2505
PshAI GACnn'nnGTC
Cuts at: 0 22 1949 3051
Size: 22 1927 1102
Fragments arranged by size:
1927 1102 22
PstI C_TGCA'G
Cuts at: 0 1590 2379 2789 3051
Size: 1590 789 410 262
Fragments arranged by size:
1590 789 410 262
PvuII CAG'CTG
Cuts at: 0 686 1236 1404 1765 1987 2231 3051
Size: 686 550 168 361 222 244 820
Fragments arranged by size:
820 686 550 361 244 222 168
SanDI GG'GwC_CC
Cuts at: 0 546 3051
Size: 546 2505
SapI GCTCTTCn'nnn_
Cuts at: 0 1105 2713 3051
Size: 1105 1608 338
Fragments arranged by size:
1608 1105 338
SbfI CC_TGCA'GG
Cuts at: 0 1590 3051
Size: 1590 1461
SexAI A'CCwGG_T
Cuts at: 0 707 1728 2564 2762 3051
Size: 707 1021 836 198 289
Fragments arranged by size:
1021 836 707 289 198
SfcI C'TryA_G
Cuts at: 0 1586 2375 2785 3051
Size: 1586 789 410 266
Fragments arranged by size:
1586 789 410 266
SfoI GGC'GCC
Cuts at: 0 1539 3051
Size: 1539 1512
SmlI C'TyrA_G
Cuts at: 0 572 1204 1891 1897 3051
Size: 572 632 687 6 1154
Fragments arranged by size:
1154 687 632 572 6
SnaBI TAC'GTA
Cuts at: 0 302 3051
Size: 302 2749
SspI AAT'ATT
Cuts at: 0 174 3051
Size: 174 2877
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1174 3051
Size: 1174 1877
TatI w'GTAC_w
Cuts at: 0 2105 3051
Size: 2105 946
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1999 2031 3051
Size: 1999 32 1020
Fragments arranged by size:
1999 1020 32
Tth111I GACn'n_nGTC
Cuts at: 0 1665 3051
Size: 1665 1386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1081 1132 2562 3051
Size: 1081 51 1430 489
Fragments arranged by size:
1430 1081 489 51
XmnI GAAnn'nnTTC
Cuts at: 0 173 3051
Size: 173 2878
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII
HindIII HpaI KasI KpnI MmeI MslI MspA1I NarI
NheI NspI PciI PfoI PpuMI PshAI PstI PvuII
SanDI SapI SbfI SexAI SfcI SfoI SmlI SnaBI
SspI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BciVI BclI BfrBI BglI BsaBI BsiWI BsrFI BssHII
BstAPI BstBI BstZ17I ClaI DraI DrdI EciI EcoICRI
EcoRI FalI FseI FspI FspAI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI ScaI SfiI
SgfI SgrAI SmaI SpeI SphI SrfI StuI StyI
SwaI XbaI XhoI XmaI ZraI