Restriction map for
pF1KB2323
(Linear) (Six-base) MAPSORT of: KIBB2323.seq Check: 3724 from: 1 to: 3063
KIBB2323.orf
With 182 enzymes: SgfI *
July 25, 2014 11:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1161 2651 3063
Size: 1161 1490 412
Fragments arranged by size:
1490 1161 412
AatII G_ACGT'C
Cuts at: 0 281 3063
Size: 281 2782
AccI GT'mk_AC
Cuts at: 0 281 637 1246 2356 3063
Size: 281 356 609 1110 707
Fragments arranged by size:
1110 707 609 356 281
Acc65I G'GTAC_C
Cuts at: 0 551 1428 3063
Size: 551 877 1635
Fragments arranged by size:
1635 877 551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 905 1030 1162 2104 2701 2804 3063
Size: 122 783 125 132 942 597 103 259
Fragments arranged by size:
942 783 597 259 132 125 122 103
AflIII A'CryG_T
Cuts at: 0 2439 3063
Size: 2439 624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1430 3063
Size: 1430 1633
AlwNI CAG_nnn'CTG
Cuts at: 0 1597 2846 3063
Size: 1597 1249 217
Fragments arranged by size:
1597 1249 217
ApaI G_GGCC'C
Cuts at: 0 632 1105 3063
Size: 632 473 1958
Fragments arranged by size:
1958 632 473
ApaLI G'TGCA_C
Cuts at: 0 1207 2587 3063
Size: 1207 1380 476
Fragments arranged by size:
1380 1207 476
ApoI r'AATT_y
Cuts at: 0 1812 1941 2034 2248 3063
Size: 1812 129 93 214 815
Fragments arranged by size:
1812 815 214 129 93
AseI AT'TA_AT
Cuts at: 0 2771 3063
Size: 2771 292
AvaI C'yCGr_G
Cuts at: 0 1369 1965 3063
Size: 1369 596 1098
Fragments arranged by size:
1369 1098 596
BamHI G'GATC_C
Cuts at: 0 768 827 3063
Size: 768 59 2236
Fragments arranged by size:
2236 768 59
BanI G'GyrC_C
Cuts at: 0 551 1117 1414 1428 1716 2639 3063
Size: 551 566 297 14 288 923 424
Fragments arranged by size:
923 566 551 424 297 288 14
BanII G_rGCy'C
Cuts at: 0 632 1049 1105 2060 2633 2828 3063
Size: 632 417 56 955 573 195 235
Fragments arranged by size:
955 632 573 417 235 195 56
BbeI G_GCGC'C
Cuts at: 0 1121 3063
Size: 1121 1942
BbsI GAAGACnn'nnnn_
Cuts at: 0 1843 2312 3044 3063
Size: 1843 469 732 19
Fragments arranged by size:
1843 732 469 19
BglII A'GATC_T
Cuts at: 0 1088 1566 3063
Size: 1088 478 1497
Fragments arranged by size:
1497 1088 478
BlpI GC'TnA_GC
Cuts at: 0 49 2523 3063
Size: 49 2474 540
Fragments arranged by size:
2474 540 49
Bme1580I G_kGCm'C
Cuts at: 0 632 1105 1211 2591 3063
Size: 632 473 106 1380 472
Fragments arranged by size:
1380 632 473 472 106
BmgBI CAC'GTC
Cuts at: 0 1733 3063
Size: 1733 1330
BmrI ACTGGGnnnn_n'
Cuts at: 0 106 688 1427 1479 2222 3063
Size: 106 582 739 52 743 841
Fragments arranged by size:
841 743 739 582 106 52
BmtI G_CTAG'C
Cuts at: 0 1754 3063
Size: 1754 1309
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1303 1595 3063
Size: 1303 292 1468
Fragments arranged by size:
1468 1303 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 700 1033 1477 1507 1630 2203 2527
Size: 316 384 333 444 30 123 573 324
Cuts at: 2527 2632 3032 3063
Size: 105 400 31
Fragments arranged by size:
573 444 400 384 333 324 316 123
105 31 30
Bpu10I CC'TnA_GC
Cuts at: 0 2632 3063
Size: 2632 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1696 2401 3063
Size: 1069 627 705 662
Fragments arranged by size:
1069 705 662 627
BsaAI yAC'GTr
Cuts at: 0 285 1206 2440 3063
Size: 285 921 1234 623
Fragments arranged by size:
1234 921 623 285
BsaBI GATnn'nnATC
Cuts at: 0 790 3063
Size: 790 2273
BsaHI Gr'CG_yC
Cuts at: 0 278 1118 3063
Size: 278 840 1945
Fragments arranged by size:
1945 840 278
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 584 2841 2890 2911 3058 3063
Size: 584 2257 49 21 147 5
Fragments arranged by size:
2257 584 147 49 21 5
BseYI C'CCAG_C
Cuts at: 0 1410 3063
Size: 1410 1653
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1426 1551 2272 2629 3063
Size: 1426 125 721 357 434
Fragments arranged by size:
1426 721 434 357 125
BsiEI CG_ry'CG
Cuts at: 0 2316 3063
Size: 2316 747
BsiHKAI G_wGCw'C
Cuts at: 0 1049 1211 2060 2480 2591 2807 3063
Size: 1049 162 849 420 111 216 256
Fragments arranged by size:
1049 849 420 256 216 162 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 269 3063
Size: 269 2794
Bsp1286I G_dGCh'C
Cuts at: 0 632 1049 1105 1211 2060 2480 2591 2633
Size: 632 417 56 106 849 420 111 42
Cuts at: 2633 2807 2828 3063
Size: 174 21 235
Fragments arranged by size:
849 632 420 417 235 174 111 106
56 42 21
BspHI T'CATG_A
Cuts at: 0 1145 3063
Size: 1145 1918
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 1161 2651 3063
Size: 191 970 1490 412
Fragments arranged by size:
1490 970 412 191
BsrBI CCG'CTC
Cuts at: 0 645 3063
Size: 645 2418
BsrDI GCAATG_nn'
Cuts at: 0 135 3063
Size: 135 2928
BsrFI r'CCGG_y
Cuts at: 0 1696 3063
Size: 1696 1367
BstAPI GCAn_nnn'nTGC
Cuts at: 0 125 264 3063
Size: 125 139 2799
Fragments arranged by size:
2799 139 125
BstBI TT'CG_AA
Cuts at: 0 1779 3063
Size: 1779 1284
BstEII G'GTnAC_C
Cuts at: 0 106 3063
Size: 106 2957
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2765 3063
Size: 2765 298
BstYI r'GATC_y
Cuts at: 0 768 827 1088 1566 2177 2843 3063
Size: 768 59 261 478 611 666 220
Fragments arranged by size:
768 666 611 478 261 220 59
BstZ17I GTA'TAC
Cuts at: 0 638 3063
Size: 638 2425
Bsu36I CC'TnA_GG
Cuts at: 0 1213 3063
Size: 1213 1850
BtgI C'CryG_G
Cuts at: 0 1351 1440 2748 3063
Size: 1351 89 1308 315
Fragments arranged by size:
1351 1308 315 89
BtsI GCAGTG_nn'
Cuts at: 0 845 930 1499 2956 3063
Size: 845 85 569 1457 107
Fragments arranged by size:
1457 845 569 107 85
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1217 1252 1287 3063
Size: 1217 35 35 1776
Fragments arranged by size:
1776 1217 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1845 3063
Size: 1845 1218
EaeI y'GGCC_r
Cuts at: 0 57 138 927 2489 3063
Size: 57 81 789 1562 574
Fragments arranged by size:
1562 789 574 81 57
EarI CTCTTCn'nnn_
Cuts at: 0 1892 2618 3063
Size: 1892 726 445
Fragments arranged by size:
1892 726 445
EcoICRI GAG'CTC
Cuts at: 0 1047 2058 3063
Size: 1047 1011 1005
Fragments arranged by size:
1047 1011 1005
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 316 700 905 1030 1033 1162 1477
Size: 122 194 384 205 125 3 129 315
Cuts at: 1477 1507 1630 2104 2203 2527 2632 2701 2804
Size: 30 123 474 99 324 105 69 103
Cuts at: 2804 3032 3063
Size: 228 31
Fragments arranged by size:
474 384 324 315 228 205 194 129
125 123 122 105 103 99 69 31
30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 3063
Size: 559 2504
EcoO109I rG'GnC_Cy
Cuts at: 0 148 629 682 1066 3063
Size: 148 481 53 384 1997
Fragments arranged by size:
1997 481 384 148 53
EcoRI G'AATT_C
Cuts at: 0 2034 3063
Size: 2034 1029
HaeII r_GCGC'y
Cuts at: 0 1121 3063
Size: 1121 1942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 582 614 753 785 1020 1052
Size: 424 32 126 32 139 32 235 32
Cuts at: 1052 1080 1112 1626 1658 3063
Size: 28 32 514 32 1405
Fragments arranged by size:
1405 514 424 235 139 126 32 32
32 32 32 32 28
HincII GTy'rAC
Cuts at: 0 858 1661 2954 3063
Size: 858 803 1293 109
Fragments arranged by size:
1293 858 803 109
HindIII A'AGCT_T
Cuts at: 0 2377 2683 3063
Size: 2377 306 380
Fragments arranged by size:
2377 380 306
HpaI GTT'AAC
Cuts at: 0 1661 3063
Size: 1661 1402
KasI G'GCGC_C
Cuts at: 0 1117 3063
Size: 1117 1946
KpnI G_GTAC'C
Cuts at: 0 555 1432 3063
Size: 555 877 1631
Fragments arranged by size:
1631 877 555
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2096 2990 3063
Size: 2096 894 73
Fragments arranged by size:
2096 894 73
MscI TGG'CCA
Cuts at: 0 59 140 2491 3063
Size: 59 81 2351 572
Fragments arranged by size:
2351 572 81 59
MslI CAynn'nnrTG
Cuts at: 0 986 1527 3063
Size: 986 541 1536
Fragments arranged by size:
1536 986 541
MspA1I CmG'CkG
Cuts at: 0 261 738 868 1410 1485 2286 2498 2796
Size: 261 477 130 542 75 801 212 298
Cuts at: 2796 3063
Size: 267
Fragments arranged by size:
801 542 477 298 267 261 212 130
75
NarI GG'CG_CC
Cuts at: 0 1118 3063
Size: 1118 1945
NcoI C'CATG_G
Cuts at: 0 1440 2748 3063
Size: 1440 1308 315
Fragments arranged by size:
1440 1308 315
NdeI CA'TA_TG
Cuts at: 0 15 1524 3063
Size: 15 1509 1539
Fragments arranged by size:
1539 1509 15
NheI G'CTAG_C
Cuts at: 0 1750 3063
Size: 1750 1313
PasI CC'CwG_GG
Cuts at: 0 625 3063
Size: 625 2438
PflMI CCAn_nnn'nTGG
Cuts at: 0 2135 3063
Size: 2135 928
PfoI T'CCnGG_A
Cuts at: 0 2180 2839 3063
Size: 2180 659 224
Fragments arranged by size:
2180 659 224
PmlI CAC'GTG
Cuts at: 0 1206 3063
Size: 1206 1857
PpuMI rG'GwC_Cy
Cuts at: 0 148 682 3063
Size: 148 534 2381
Fragments arranged by size:
2381 534 148
PsiI TTA'TAA
Cuts at: 0 2206 3063
Size: 2206 857
PspOMI G'GGCC_C
Cuts at: 0 628 1101 3063
Size: 628 473 1962
Fragments arranged by size:
1962 628 473
PstI C_TGCA'G
Cuts at: 0 2503 3063
Size: 2503 560
PvuI CG_AT'CG
Cuts at: 0 2316 3063
Size: 2316 747
PvuII CAG'CTG
Cuts at: 0 738 868 1410 1485 2286 3063
Size: 738 130 542 75 801 777
Fragments arranged by size:
801 777 738 542 130 75
SacI G_AGCT'C
Cuts at: 0 1049 2060 3063
Size: 1049 1011 1003
Fragments arranged by size:
1049 1011 1003
SanDI GG'GwC_CC
Cuts at: 0 682 3063
Size: 682 2381
SexAI A'CCwGG_T
Cuts at: 0 547 3063
Size: 547 2516
SfcI C'TryA_G
Cuts at: 0 1122 2499 2666 3063
Size: 1122 1377 167 397
Fragments arranged by size:
1377 1122 397 167
SfoI GGC'GCC
Cuts at: 0 1119 3063
Size: 1119 1944
SmlI C'TyrA_G
Cuts at: 0 1084 1711 2416 3063
Size: 1084 627 705 647
Fragments arranged by size:
1084 705 647 627
StyI C'CwwG_G
Cuts at: 0 224 1070 1440 2748 3063
Size: 224 846 370 1308 315
Fragments arranged by size:
1308 846 370 315 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 823 1402 1801 2003 2476 2771 2982 3063
Size: 823 579 399 202 473 295 211 81
Fragments arranged by size:
823 579 473 399 295 211 202 81
TatI w'GTAC_w
Cuts at: 0 2453 2534 2717 3063
Size: 2453 81 183 346
Fragments arranged by size:
2453 346 183 81
Tth111I GACn'n_nGTC
Cuts at: 0 2674 3063
Size: 2674 389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 779 1482 3063
Size: 779 703 1581
Fragments arranged by size:
1581 779 703
XmnI GAAnn'nnTTC
Cuts at: 0 761 3063
Size: 761 2302
ZraI GAC'GTC
Cuts at: 0 279 3063
Size: 279 2784
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI CspCI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PstI PvuI PvuII SacI SanDI
SexAI SfcI SfoI SmlI StyI TaqII TatI Tth111I
XcmI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AloI AscI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglI BsaI BsaWI BsaXI BsiWI BsmI BspEI BsrGI
BssHII BssSI BtgZI ClaI DraI DraIII EagI EciI
EcoRV FalI FseI FspI FspAI MfeI MluI NaeI
NgoMIV NotI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PspXI PsrI RsrII SacII SalI SapI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XhoI
XmaI