Restriction map for
pF1KB2497
(Linear) (Six-base) MAPSORT of: KIBB2497.seq Check: 4876 from: 1 to: 2409
KIBB2497.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 881 980 1340 1622 2409
Size: 881 99 360 282 787
Fragments arranged by size:
881 787 360 282 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 2409
Size: 883 1526
AflII C'TTAA_G
Cuts at: 0 1413 2409
Size: 1413 996
AhdI GACnn_n'nnGTC
Cuts at: 0 1986 2409
Size: 1986 423
AleI CACnn'nnGTG
Cuts at: 0 662 1039 2409
Size: 662 377 1370
Fragments arranged by size:
1370 662 377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1961 2409
Size: 1961 448
AlwNI CAG_nnn'CTG
Cuts at: 0 343 525 1266 2409
Size: 343 182 741 1143
Fragments arranged by size:
1143 741 343 182
ApaI G_GGCC'C
Cuts at: 0 148 164 205 373 416 754 772 2409
Size: 148 16 41 168 43 338 18 1637
Fragments arranged by size:
1637 338 168 148 43 41 18 16
ApaLI G'TGCA_C
Cuts at: 0 868 2409
Size: 868 1541
ApoI r'AATT_y
Cuts at: 0 1325 1755 2409
Size: 1325 430 654
Fragments arranged by size:
1325 654 430
AvaI C'yCGr_G
Cuts at: 0 163 372 613 660 820 2409
Size: 163 209 241 47 160 1589
Fragments arranged by size:
1589 241 209 163 160 47
BanI G'GyrC_C
Cuts at: 0 222 729 968 1937 2409
Size: 222 507 239 969 472
Fragments arranged by size:
969 507 472 239 222
BanII G_rGCy'C
Cuts at: 0 148 164 205 325 373 380 416 754
Size: 148 16 41 120 48 7 36 338
Cuts at: 754 772 790 1924 2074 2409
Size: 18 18 1134 150 335
Fragments arranged by size:
1134 338 335 150 148 120 48 41
36 18 18 16 7
BbeI G_GCGC'C
Cuts at: 0 226 733 2409
Size: 226 507 1676
Fragments arranged by size:
1676 507 226
BbsI GAAGACnn'nnnn_
Cuts at: 0 1210 1699 2409
Size: 1210 489 710
Fragments arranged by size:
1210 710 489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1140 1174 2409
Size: 1140 34 1235
Fragments arranged by size:
1235 1140 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 70 2019 2409
Size: 70 1949 390
Fragments arranged by size:
1949 390 70
BglI GCCn_nnn'nGGC
Cuts at: 0 168 190 2409
Size: 168 22 2219
Fragments arranged by size:
2219 168 22
BglII A'GATC_T
Cuts at: 0 2373 2409
Size: 2373 36
BlpI GC'TnA_GC
Cuts at: 0 701 1739 2409
Size: 701 1038 670
Fragments arranged by size:
1038 701 670
Bme1580I G_kGCm'C
Cuts at: 0 148 164 205 373 416 502 754 772
Size: 148 16 41 168 43 86 252 18
Cuts at: 772 820 872 1459 1531 1940 2409
Size: 48 52 587 72 409 469
Fragments arranged by size:
587 469 409 252 168 148 86 72
52 48 43 41 18 16
BmgBI CAC'GTC
Cuts at: 0 309 2409
Size: 309 2100
BmrI ACTGGGnnnn_n'
Cuts at: 0 2316 2409
Size: 2316 93
BmtI G_CTAG'C
Cuts at: 0 700 2409
Size: 700 1709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1943 2409
Size: 1943 466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 2133 2409
Size: 364 1769 276
Fragments arranged by size:
1769 364 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1036 2079 2409
Size: 946 90 1043 330
Fragments arranged by size:
1043 946 330 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 1347 2097 2409
Size: 307 1040 750 312
Fragments arranged by size:
1040 750 312 307
BsaHI Gr'CG_yC
Cuts at: 0 223 548 730 2409
Size: 223 325 182 1679
Fragments arranged by size:
1679 325 223 182
BsaWI w'CCGG_w
Cuts at: 0 325 1442 2409
Size: 325 1117 967
Fragments arranged by size:
1117 967 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1585 1615 2409
Size: 1585 30 794
Fragments arranged by size:
1585 794 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 370 379 751 754 1123 1644 2003
Size: 193 177 9 372 3 369 521 359
Cuts at: 2003 2409
Size: 406
Fragments arranged by size:
521 406 372 369 359 193 177 9
3
BseYI C'CCAG_C
Cuts at: 0 605 637 783 1768 2176 2409
Size: 605 32 146 985 408 233
Fragments arranged by size:
985 605 408 233 146 32
BsiEI CG_ry'CG
Cuts at: 0 78 710 758 2409
Size: 78 632 48 1651
Fragments arranged by size:
1651 632 78 48
BsiHKAI G_wGCw'C
Cuts at: 0 872 1227 1924 2074 2224 2409
Size: 872 355 697 150 150 185
Fragments arranged by size:
872 697 355 185 150 150
Bsp1286I G_dGCh'C
Cuts at: 0 148 164 205 325 373 380 416 502
Size: 148 16 41 120 48 7 36 86
Cuts at: 502 754 772 790 820 872 1227 1459 1531
Size: 252 18 18 30 52 355 232 72
Cuts at: 1531 1924 1940 2074 2224 2409
Size: 393 16 134 150 185
Fragments arranged by size:
393 355 252 232 185 150 148 134
120 86 72 52 48 41 36 30
18 18 16 16 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 533 881 980 1340 1622 2409
Size: 533 348 99 360 282 787
Fragments arranged by size:
787 533 360 348 282 99
BsrBI CCG'CTC
Cuts at: 0 395 598 2409
Size: 395 203 1811
Fragments arranged by size:
1811 395 203
BsrDI GCAATG_nn'
Cuts at: 0 1861 2409
Size: 1861 548
BsrFI r'CCGG_y
Cuts at: 0 102 705 1345 2409
Size: 102 603 640 1064
Fragments arranged by size:
1064 640 603 102
BsrGI T'GTAC_A
Cuts at: 0 1043 2409
Size: 1043 1366
BssSI C'ACGA_G
Cuts at: 0 1485 2409
Size: 1485 924
BstBI TT'CG_AA
Cuts at: 0 1160 2409
Size: 1160 1249
BstXI CCAn_nnnn'nTGG
Cuts at: 0 581 2409
Size: 581 1828
BstYI r'GATC_y
Cuts at: 0 1728 2119 2373 2409
Size: 1728 391 254 36
Fragments arranged by size:
1728 391 254 36
Bsu36I CC'TnA_GG
Cuts at: 0 734 2162 2409
Size: 734 1428 247
Fragments arranged by size:
1428 734 247
BtgI C'CryG_G
Cuts at: 0 65 317 407 443 485 774 2352 2409
Size: 65 252 90 36 42 289 1578 57
Fragments arranged by size:
1578 289 252 90 65 57 42 36
BtsI GCAGTG_nn'
Cuts at: 0 416 835 1779 2409
Size: 416 419 944 630
Fragments arranged by size:
944 630 419 416
EaeI y'GGCC_r
Cuts at: 0 75 108 560 707 755 1997 2051 2135
Size: 75 33 452 147 48 1242 54 84
Cuts at: 2135 2409
Size: 274
Fragments arranged by size:
1242 452 274 147 84 75 54 48
33
EagI C'GGCC_G
Cuts at: 0 75 707 755 2409
Size: 75 632 48 1654
Fragments arranged by size:
1654 632 75 48
EarI CTCTTCn'nnn_
Cuts at: 0 1675 1834 2409
Size: 1675 159 575
Fragments arranged by size:
1675 575 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 459 760 1507 2409
Size: 459 301 747 902
Fragments arranged by size:
902 747 459 301
EcoICRI GAG'CTC
Cuts at: 0 1922 2072 2409
Size: 1922 150 337
Fragments arranged by size:
1922 337 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 883 2133 2409
Size: 364 519 1250 276
Fragments arranged by size:
1250 519 364 276
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1463 1553 2409
Size: 1463 90 856
Fragments arranged by size:
1463 856 90
EcoO109I rG'GnC_Cy
Cuts at: 0 91 114 144 145 201 202 340 369
Size: 91 23 30 1 56 1 138 29
Cuts at: 369 412 427 522 750 769 905 2409
Size: 43 15 95 228 19 136 1504
Fragments arranged by size:
1504 228 138 136 95 91 56 43
30 29 23 19 15 1 1
HaeII r_GCGC'y
Cuts at: 0 226 733 1142 2409
Size: 226 507 409 1267
Fragments arranged by size:
1267 507 409 226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 1807 1809 1839 1841 1972 2004
Size: 219 32 1556 2 30 2 131 32
Cuts at: 2004 2077 2109 2409
Size: 73 32 300
Fragments arranged by size:
1556 300 219 131 73 32 32 32
30 2 2
KasI G'GCGC_C
Cuts at: 0 222 729 2409
Size: 222 507 1680
Fragments arranged by size:
1680 507 222
MscI TGG'CCA
Cuts at: 0 110 562 1999 2137 2409
Size: 110 452 1437 138 272
Fragments arranged by size:
1437 452 272 138 110
MslI CAynn'nnrTG
Cuts at: 0 662 1039 1092 1399 1436 2409
Size: 662 377 53 307 37 973
Fragments arranged by size:
973 662 377 307 53 37
MspA1I CmG'CkG
Cuts at: 0 67 319 409 434 445 487 776 783
Size: 67 252 90 25 11 42 289 7
Cuts at: 783 861 2409
Size: 78 1548
Fragments arranged by size:
1548 289 252 90 78 67 42 25
11 7
NaeI GCC'GGC
Cuts at: 0 104 707 2409
Size: 104 603 1702
Fragments arranged by size:
1702 603 104
NarI GG'CG_CC
Cuts at: 0 223 730 2409
Size: 223 507 1679
Fragments arranged by size:
1679 507 223
NcoI C'CATG_G
Cuts at: 0 2352 2409
Size: 2352 57
NgoMIV G'CCGG_C
Cuts at: 0 102 705 2409
Size: 102 603 1704
Fragments arranged by size:
1704 603 102
NheI G'CTAG_C
Cuts at: 0 696 2409
Size: 696 1713
NspI r_CATG'y
Cuts at: 0 652 1404 2409
Size: 652 752 1005
Fragments arranged by size:
1005 752 652
PasI CC'CwG_GG
Cuts at: 0 765 921 2409
Size: 765 156 1488
Fragments arranged by size:
1488 765 156
PpuMI rG'GwC_Cy
Cuts at: 0 114 522 2409
Size: 114 408 1887
Fragments arranged by size:
1887 408 114
PspOMI G'GGCC_C
Cuts at: 0 144 160 201 369 412 750 768 2409
Size: 144 16 41 168 43 338 18 1641
Fragments arranged by size:
1641 338 168 144 43 41 18 16
PstI C_TGCA'G
Cuts at: 0 977 1294 1619 2409
Size: 977 317 325 790
Fragments arranged by size:
977 790 325 317
PvuII CAG'CTG
Cuts at: 0 861 2409
Size: 861 1548
RsrII CG'GwC_CG
Cuts at: 0 778 2409
Size: 778 1631
SacI G_AGCT'C
Cuts at: 0 1924 2074 2409
Size: 1924 150 335
Fragments arranged by size:
1924 335 150
SacII CC_GC'GG
Cuts at: 0 68 320 410 446 488 777 2409
Size: 68 252 90 36 42 289 1632
Fragments arranged by size:
1632 289 252 90 68 42 36
SbfI CC_TGCA'GG
Cuts at: 0 1619 2409
Size: 1619 790
ScaI AGT'ACT
Cuts at: 0 1851 2409
Size: 1851 558
SfcI C'TryA_G
Cuts at: 0 37 973 1290 1615 2409
Size: 37 936 317 325 794
Fragments arranged by size:
936 794 325 317 37
SfoI GGC'GCC
Cuts at: 0 224 731 2409
Size: 224 507 1678
Fragments arranged by size:
1678 507 224
SgrAI Cr'CCGG_yG
Cuts at: 0 1345 2409
Size: 1345 1064
SmaI CCC'GGG
Cuts at: 0 165 374 615 662 2409
Size: 165 209 241 47 1747
Fragments arranged by size:
1747 241 209 165 47
SmlI C'TyrA_G
Cuts at: 0 961 1051 1413 2094 2409
Size: 961 90 362 681 315
Fragments arranged by size:
961 681 362 315 90
SphI G_CATG'C
Cuts at: 0 652 2409
Size: 652 1757
SrfI GCCC'GGGC
Cuts at: 0 165 2409
Size: 165 2244
StuI AGG'CCT
Cuts at: 0 1173 2409
Size: 1173 1236
StyI C'CwwG_G
Cuts at: 0 2352 2409
Size: 2352 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 289 913 1782 2190 2409
Size: 247 42 624 869 408 219
Fragments arranged by size:
869 624 408 247 219 42
TatI w'GTAC_w
Cuts at: 0 809 1043 1849 2409
Size: 809 234 806 560
Fragments arranged by size:
809 806 560 234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 578 2409
Size: 578 1831
XmaI C'CCGG_G
Cuts at: 0 163 372 613 660 2409
Size: 163 209 241 47 1749
Fragments arranged by size:
1749 241 209 163 47
XmnI GAAnn'nnTTC
Cuts at: 0 1087 1480 2409
Size: 1087 393 929
Fragments arranged by size:
1087 929 393
Enzymes that do cut:
AarI AcuI AflII AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstBI BstXI BstYI Bsu36I BtgI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PpuMI PspOMI PstI
PvuII RsrII SacI SacII SbfI ScaI SfcI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BclI
BfrBI Bpu10I BsaAI BsaBI BsgI BsiWI BsmI BsmBI
BspEI BspHI BssHII BstAPI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI SalI SanDI SapI SexAI SfiI SgfI SnaBI
SpeI SspI SwaI TstI Tth111I XbaI XhoI ZraI