Restriction map for
pF1KSDB2506
(Linear) (Six-base) MAPSORT of: KIBB2506.seq Check: 4580 from: 1 to: 1209
KIBB2506.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AfeI AGC'GCT
Cuts at: 0 1104 1209
Size: 1104 105
AflIII A'CryG_T
Cuts at: 0 134 926 1121 1209
Size: 134 792 195 88
Fragments arranged by size:
792 195 134 88
AleI CACnn'nnGTG
Cuts at: 0 73 1209
Size: 73 1136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 809 904 1209
Size: 809 95 305
Fragments arranged by size:
809 305 95
AlwNI CAG_nnn'CTG
Cuts at: 0 839 1209
Size: 839 370
ApaI G_GGCC'C
Cuts at: 0 469 1209
Size: 469 740
AvaI C'yCGr_G
Cuts at: 0 101 1209
Size: 101 1108
BanI G'GyrC_C
Cuts at: 0 196 1209
Size: 196 1013
BanII G_rGCy'C
Cuts at: 0 33 274 469 590 1209
Size: 33 241 195 121 619
Fragments arranged by size:
619 241 195 121 33
BbeI G_GCGC'C
Cuts at: 0 200 1209
Size: 200 1009
BbsI GAAGACnn'nnnn_
Cuts at: 0 837 1203 1209
Size: 837 366 6
Fragments arranged by size:
837 366 6
BclI T'GATC_A
Cuts at: 0 65 203 1209
Size: 65 138 1006
Fragments arranged by size:
1006 138 65
BglI GCCn_nnn'nGGC
Cuts at: 0 278 473 1209
Size: 278 195 736
Fragments arranged by size:
736 278 195
BlpI GC'TnA_GC
Cuts at: 0 421 1209
Size: 421 788
Bme1580I G_kGCm'C
Cuts at: 0 469 1209
Size: 469 740
BmgBI CAC'GTC
Cuts at: 0 929 969 1209
Size: 929 40 240
Fragments arranged by size:
929 240 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 1070 1209
Size: 1070 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 1209
Size: 349 860
BsaAI yAC'GTr
Cuts at: 0 135 1209
Size: 135 1074
BsaBI GATnn'nnATC
Cuts at: 0 223 1209
Size: 223 986
BsaHI Gr'CG_yC
Cuts at: 0 155 197 389 1209
Size: 155 42 192 820
Fragments arranged by size:
820 192 155 42
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 697 976 1209
Size: 112 585 279 233
Fragments arranged by size:
585 279 233 112
BseYI C'CCAG_C
Cuts at: 0 839 1099 1209
Size: 839 260 110
Fragments arranged by size:
839 260 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 991 1209
Size: 864 127 218
Fragments arranged by size:
864 218 127
BsiHKAI G_wGCw'C
Cuts at: 0 33 44 548 734 1154 1209
Size: 33 11 504 186 420 55
Fragments arranged by size:
504 420 186 55 33 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 943 1209
Size: 943 266
Bsp1286I G_dGCh'C
Cuts at: 0 33 44 274 469 548 590 734 1154
Size: 33 11 230 195 79 42 144 420
Cuts at: 1154 1209
Size: 55
Fragments arranged by size:
420 230 195 144 79 55 42 33
11
BsrDI GCAATG_nn'
Cuts at: 0 777 1209
Size: 777 432
BstEII G'GTnAC_C
Cuts at: 0 636 1209
Size: 636 573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1209
Size: 825 384
BstYI r'GATC_y
Cuts at: 0 872 1209
Size: 872 337
BtgI C'CryG_G
Cuts at: 0 470 590 979 1010 1209
Size: 470 120 389 31 199
Fragments arranged by size:
470 389 199 120 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 510 1209
Size: 510 699
BtsI GCAGTG_nn'
Cuts at: 0 208 876 1209
Size: 208 668 333
Fragments arranged by size:
668 333 208
DraIII CAC_nnn'GTG
Cuts at: 0 39 665 1209
Size: 39 626 544
Fragments arranged by size:
626 544 39
EaeI y'GGCC_r
Cuts at: 0 185 815 830 1142 1209
Size: 185 630 15 312 67
Fragments arranged by size:
630 312 185 67 15
EarI CTCTTCn'nnn_
Cuts at: 0 64 262 661 769 1209
Size: 64 198 399 108 440
Fragments arranged by size:
440 399 198 108 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 361 1169 1209
Size: 361 808 40
Fragments arranged by size:
808 361 40
EcoICRI GAG'CTC
Cuts at: 0 31 1209
Size: 31 1178
EcoO109I rG'GnC_Cy
Cuts at: 0 305 466 698 1209
Size: 305 161 232 511
Fragments arranged by size:
511 305 232 161
HaeII r_GCGC'y
Cuts at: 0 200 499 1106 1209
Size: 200 299 607 103
Fragments arranged by size:
607 299 200 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 1209
Size: 15 32 1162
Fragments arranged by size:
1162 32 15
KasI G'GCGC_C
Cuts at: 0 196 1209
Size: 196 1013
MscI TGG'CCA
Cuts at: 0 817 1209
Size: 817 392
MslI CAynn'nnrTG
Cuts at: 0 73 172 529 739 1209
Size: 73 99 357 210 470
Fragments arranged by size:
470 357 210 99 73
MspA1I CmG'CkG
Cuts at: 0 472 483 836 975 981 1209
Size: 472 11 353 139 6 228
Fragments arranged by size:
472 353 228 139 11 6
NarI GG'CG_CC
Cuts at: 0 197 1209
Size: 197 1012
NcoI C'CATG_G
Cuts at: 0 590 1010 1209
Size: 590 420 199
Fragments arranged by size:
590 420 199
PasI CC'CwG_GG
Cuts at: 0 309 642 1209
Size: 309 333 567
Fragments arranged by size:
567 333 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 839 1209
Size: 839 370
PfoI T'CCnGG_A
Cuts at: 0 344 1209
Size: 344 865
PpuMI rG'GwC_Cy
Cuts at: 0 305 1209
Size: 305 904
PspOMI G'GGCC_C
Cuts at: 0 465 1209
Size: 465 744
PstI C_TGCA'G
Cuts at: 0 785 902 1209
Size: 785 117 307
Fragments arranged by size:
785 307 117
PvuII CAG'CTG
Cuts at: 0 483 975 1209
Size: 483 492 234
Fragments arranged by size:
492 483 234
SacI G_AGCT'C
Cuts at: 0 33 1209
Size: 33 1176
SacII CC_GC'GG
Cuts at: 0 473 982 1209
Size: 473 509 227
Fragments arranged by size:
509 473 227
SanDI GG'GwC_CC
Cuts at: 0 305 1209
Size: 305 904
SapI GCTCTTCn'nnn_
Cuts at: 0 64 262 1209
Size: 64 198 947
Fragments arranged by size:
947 198 64
SbfI CC_TGCA'GG
Cuts at: 0 902 1209
Size: 902 307
SexAI A'CCwGG_T
Cuts at: 0 35 1209
Size: 35 1174
SfcI C'TryA_G
Cuts at: 0 781 898 1209
Size: 781 117 311
Fragments arranged by size:
781 311 117
SfoI GGC'GCC
Cuts at: 0 198 1209
Size: 198 1011
StuI AGG'CCT
Cuts at: 0 1003 1209
Size: 1003 206
StyI C'CwwG_G
Cuts at: 0 590 812 1010 1209
Size: 590 222 198 199
Fragments arranged by size:
590 222 199 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 729 1209
Size: 729 480
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1059 1083 1209
Size: 1059 24 126
Fragments arranged by size:
1059 126 24
Enzymes that do cut:
AfeI AflIII AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BclI BglI BlpI Bme1580I BmgBI
BmrI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BsrDI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI EcoO109I
HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SapI SbfI SexAI SfcI SfoI StuI
StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BmtI
BplI BpmI Bpu10I BpuEI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI