(Linear) (Six-base) MAPSORT of: KIBB2507.seq  Check: 841  from: 1  to: 1182



KIBB2507.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     561     593    1182 
   Size:        561      32     589

  Fragments arranged by size: 

                 589     561      32
 
AseI AT'TA_AT

Cuts at:      0     362     374    1182 
   Size:        362      12     808

  Fragments arranged by size: 

                 808     362      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      30    1182 
   Size:         30    1152
 
BclI T'GATC_A

Cuts at:      0     818    1182 
   Size:        818     364
 
BfrBI ATG'CAT

Cuts at:      0      75    1182 
   Size:         75    1107
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1143    1182 
   Size:       1143      39
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746    1182 
   Size:        746     436
 
BsaAI yAC'GTr

Cuts at:      0     519    1182 
   Size:        519     663
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1116    1146    1182 
   Size:       1116      30      36

  Fragments arranged by size: 

                1116      36      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     251    1182 
   Size:        251     931
 
BsmI GAATG_Cn'

Cuts at:      0     889    1182 
   Size:        889     293
 
Bsp1286I G_dGCh'C

Cuts at:      0     251    1182 
   Size:        251     931
 
BspHI T'CATG_A

Cuts at:      0     267    1182 
   Size:        267     915
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     546    1182 
   Size:        546     636
 
BsrDI GCAATG_nn'

Cuts at:      0     695     758     776     963    1182 
   Size:        695      63      18     187     219

  Fragments arranged by size: 

                 695     219     187      63      18
 
BsrGI T'GTAC_A

Cuts at:      0     258    1182 
   Size:        258     924
 
BstYI r'GATC_y

Cuts at:      0     186    1182 
   Size:        186     996
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     747     782    1182 
   Size:        747      35     400

  Fragments arranged by size: 

                 747     400      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     927    1025    1182 
   Size:        927      98     157

  Fragments arranged by size: 

                 927     157      98
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     630    1182 
   Size:        630     552
 
EaeI y'GGCC_r

Cuts at:      0      32     716    1182 
   Size:         32     684     466

  Fragments arranged by size: 

                 684     466      32
 
EarI CTCTTCn'nnn_

Cuts at:      0      58     167    1182 
   Size:         58     109    1015

  Fragments arranged by size: 

                1015     109      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1143    1182 
   Size:       1143      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1107    1182 
   Size:       1107      75
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     268    1182 
   Size:        268     914
 
HaeII r_GCGC'y

Cuts at:      0       7    1182 
   Size:          7    1175
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      92     124     575     607    1116    1148    1182 
   Size:         92      32     451      32     509      32      34

  Fragments arranged by size: 

                 509     451      92      34      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     298    1182 
   Size:        298     884
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     638     721    1182 
   Size:        638      83     461

  Fragments arranged by size: 

                 638     461      83
 
MscI TGG'CCA

Cuts at:      0     718    1182 
   Size:        718     464
 
NsiI A_TGCA'T

Cuts at:      0      77    1182 
   Size:         77    1105
 
NspI r_CATG'y

Cuts at:      0     849    1182 
   Size:        849     333
 
PpuMI rG'GwC_Cy

Cuts at:      0    1107    1182 
   Size:       1107      75
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     485     517    1182 
   Size:        485      32     665

  Fragments arranged by size: 

                 665     485      32
 
SanDI GG'GwC_CC

Cuts at:      0    1107    1182 
   Size:       1107      75
 
SfcI C'TryA_G

Cuts at:      0    1137    1182 
   Size:       1137      45
 
SmlI C'TyrA_G

Cuts at:      0     761    1182 
   Size:        761     421
 
SnaBI TAC'GTA

Cuts at:      0     519    1182 
   Size:        519     663
 
StyI C'CwwG_G

Cuts at:      0      20    1182 
   Size:         20    1162
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     749    1182 
   Size:        749     433
 
TatI w'GTAC_w

Cuts at:      0     258     692    1182 
   Size:        258     434     490

  Fragments arranged by size: 

                 490     434     258
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1118    1182 
   Size:       1118      64
 

 Enzymes that do cut:

     AloI     AseI     BbsI     BclI    BfrBI     BpmI    BpuEI    BsaAI
    BsaXI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI
    BstYI    CspCI   DraIII     DrdI     EaeI     EarI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I  HindIII     MmeI     MscI     NsiI     NspI
    PpuMI     PsrI    SanDI     SfcI     SmlI    SnaBI     StyI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI