Restriction map for
pF1KB3002
(Linear) (Six-base) MAPSORT of: KIBB3002.seq Check: 3788 from: 1 to: 1725
KIBB3002.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 1283 1725
Size: 1283 442
Acc65I G'GTAC_C
Cuts at: 0 1055 1725
Size: 1055 670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 469 1150 1314 1662 1725
Size: 340 129 681 164 348 63
Fragments arranged by size:
681 348 340 164 129 63
AleI CACnn'nnGTG
Cuts at: 0 1603 1725
Size: 1603 122
AlwNI CAG_nnn'CTG
Cuts at: 0 697 1725
Size: 697 1028
ApaI G_GGCC'C
Cuts at: 0 138 232 828 1725
Size: 138 94 596 897
Fragments arranged by size:
897 596 138 94
ApaLI G'TGCA_C
Cuts at: 0 722 1725
Size: 722 1003
ApoI r'AATT_y
Cuts at: 0 44 1725
Size: 44 1681
AvaI C'yCGr_G
Cuts at: 0 167 312 1725
Size: 167 145 1413
Fragments arranged by size:
1413 167 145
AvrII C'CTAG_G
Cuts at: 0 85 1725
Size: 85 1640
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1308 1341 1725
Size: 1308 33 384
Fragments arranged by size:
1308 384 33
BamHI G'GATC_C
Cuts at: 0 3 1725
Size: 3 1722
BanI G'GyrC_C
Cuts at: 0 466 552 1055 1454 1460 1479 1725
Size: 466 86 503 399 6 19 246
Fragments arranged by size:
503 466 399 246 86 19 6
BanII G_rGCy'C
Cuts at: 0 127 138 168 232 282 828 1010 1554
Size: 127 11 30 64 50 546 182 544
Cuts at: 1554 1725
Size: 171
Fragments arranged by size:
546 544 182 171 127 64 50 30
11
BbeI G_GCGC'C
Cuts at: 0 1464 1725
Size: 1464 261
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 1342 1668 1725
Size: 1088 254 326 57
Fragments arranged by size:
1088 326 254 57
BbvCI CC'TCA_GC
Cuts at: 0 1594 1725
Size: 1594 131
BciVI GTATCCnnnnn_n'
Cuts at: 0 913 1725
Size: 913 812
BfrBI ATG'CAT
Cuts at: 0 58 1725
Size: 58 1667
BglI GCCn_nnn'nGGC
Cuts at: 0 1449 1725
Size: 1449 276
BlpI GC'TnA_GC
Cuts at: 0 121 320 343 1712 1725
Size: 121 199 23 1369 13
Fragments arranged by size:
1369 199 121 23 13
Bme1580I G_kGCm'C
Cuts at: 0 138 232 726 828 1484 1506 1725
Size: 138 94 494 102 656 22 219
Fragments arranged by size:
656 494 219 138 102 94 22
BmgBI CAC'GTC
Cuts at: 0 1287 1725
Size: 1287 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 398 1725
Size: 398 1327
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1029 1725
Size: 1029 696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1725
Size: 970 755
Bpu10I CC'TnA_GC
Cuts at: 0 1594 1725
Size: 1594 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 717 841 1725
Size: 688 29 124 884
Fragments arranged by size:
884 688 124 29
BsaHI Gr'CG_yC
Cuts at: 0 1461 1725
Size: 1461 264
BsaWI w'CCGG_w
Cuts at: 0 1610 1725
Size: 1610 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 646 676 1725
Size: 646 30 1049
Fragments arranged by size:
1049 646 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 378 491 494 497 500 521 1183 1408
Size: 378 113 3 3 3 21 662 225
Cuts at: 1408 1725
Size: 317
Fragments arranged by size:
662 378 317 225 113 21 3 3
3
BseYI C'CCAG_C
Cuts at: 0 302 430 569 690 1030 1112 1725
Size: 302 128 139 121 340 82 613
Fragments arranged by size:
613 340 302 139 128 121 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1511 1725
Size: 641 870 214
Fragments arranged by size:
870 641 214
BsiHKAI G_wGCw'C
Cuts at: 0 127 321 726 1010 1292 1725
Size: 127 194 405 284 282 433
Fragments arranged by size:
433 405 284 282 194 127
Bsp1286I G_dGCh'C
Cuts at: 0 127 138 168 232 282 321 726 828
Size: 127 11 30 64 50 39 405 102
Cuts at: 828 1010 1292 1484 1506 1554 1725
Size: 182 282 192 22 48 171
Fragments arranged by size:
405 282 192 182 171 127 102 64
50 48 39 30 22 11
BspEI T'CCGG_A
Cuts at: 0 1610 1725
Size: 1610 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1716 1725
Size: 1716 9
BsrBI CCG'CTC
Cuts at: 0 956 1541 1716 1725
Size: 956 585 175 9
Fragments arranged by size:
956 585 175 9
BsrFI r'CCGG_y
Cuts at: 0 1310 1430 1457 1519 1725
Size: 1310 120 27 62 206
Fragments arranged by size:
1310 206 120 62 27
BstYI r'GATC_y
Cuts at: 0 3 944 1725
Size: 3 941 781
Fragments arranged by size:
941 781 3
BtgI C'CryG_G
Cuts at: 0 1620 1725
Size: 1620 105
BtsI GCAGTG_nn'
Cuts at: 0 1598 1725
Size: 1598 127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 648 683 1725
Size: 648 35 1042
Fragments arranged by size:
1042 648 35
DraIII CAC_nnn'GTG
Cuts at: 0 249 1725
Size: 249 1476
EaeI y'GGCC_r
Cuts at: 0 207 648 788 1308 1556 1641 1725
Size: 207 441 140 520 248 85 84
Fragments arranged by size:
520 441 248 207 140 85 84
EarI CTCTTCn'nnn_
Cuts at: 0 89 107 1015 1725
Size: 89 18 908 710
Fragments arranged by size:
908 710 89 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 1725
Size: 274 1451
EcoICRI GAG'CTC
Cuts at: 0 125 1008 1725
Size: 125 883 717
Fragments arranged by size:
883 717 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 469 970 1150 1314 1662 1725
Size: 340 129 501 180 164 348 63
Fragments arranged by size:
501 348 340 180 164 129 63
EcoO109I rG'GnC_Cy
Cuts at: 0 228 477 824 825 1636 1725
Size: 228 249 347 1 811 89
Fragments arranged by size:
811 347 249 228 89 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1692 1725
Size: 1692 33
FspI TGC'GCA
Cuts at: 0 148 1725
Size: 148 1577
FspAI rTGC'GCAy
Cuts at: 0 148 1725
Size: 148 1577
HaeII r_GCGC'y
Cuts at: 0 1464 1725
Size: 1464 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 377 409 1297 1329 1725
Size: 46 32 299 32 888 32 396
Fragments arranged by size:
888 396 299 46 32 32 32
HincII GTy'rAC
Cuts at: 0 1284 1725
Size: 1284 441
KasI G'GCGC_C
Cuts at: 0 1460 1725
Size: 1460 265
KpnI G_GTAC'C
Cuts at: 0 1059 1725
Size: 1059 666
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1725
Size: 1040 685
MscI TGG'CCA
Cuts at: 0 650 790 1558 1725
Size: 650 140 768 167
Fragments arranged by size:
768 650 167 140
MslI CAynn'nnrTG
Cuts at: 0 53 250 983 1139 1304 1474 1477 1603
Size: 53 197 733 156 165 170 3 126
Cuts at: 1603 1725
Size: 122
Fragments arranged by size:
733 197 170 165 156 126 122 53
3
MspA1I CmG'CkG
Cuts at: 0 306 324 625 1725
Size: 306 18 301 1100
Fragments arranged by size:
1100 306 301 18
NaeI GCC'GGC
Cuts at: 0 1432 1725
Size: 1432 293
NarI GG'CG_CC
Cuts at: 0 1461 1725
Size: 1461 264
NcoI C'CATG_G
Cuts at: 0 1620 1725
Size: 1620 105
NgoMIV G'CCGG_C
Cuts at: 0 1430 1725
Size: 1430 295
NsiI A_TGCA'T
Cuts at: 0 60 1725
Size: 60 1665
NspI r_CATG'y
Cuts at: 0 1244 1591 1725
Size: 1244 347 134
Fragments arranged by size:
1244 347 134
PasI CC'CwG_GG
Cuts at: 0 8 225 828 907 1163 1438 1725
Size: 8 217 603 79 256 275 287
Fragments arranged by size:
603 287 275 256 217 79 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 1022 1725
Size: 1022 703
PfoI T'CCnGG_A
Cuts at: 0 704 947 1148 1725
Size: 704 243 201 577
Fragments arranged by size:
704 577 243 201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1577 1609 1725
Size: 1577 32 116
Fragments arranged by size:
1577 116 32
PspOMI G'GGCC_C
Cuts at: 0 134 228 824 1725
Size: 134 94 596 901
Fragments arranged by size:
901 596 134 94
PstI C_TGCA'G
Cuts at: 0 901 1725
Size: 901 824
PvuII CAG'CTG
Cuts at: 0 306 324 625 1725
Size: 306 18 301 1100
Fragments arranged by size:
1100 306 301 18
SacI G_AGCT'C
Cuts at: 0 127 1010 1725
Size: 127 883 715
Fragments arranged by size:
883 715 127
SalI G'TCGA_C
Cuts at: 0 1282 1725
Size: 1282 443
SapI GCTCTTCn'nnn_
Cuts at: 0 1015 1725
Size: 1015 710
ScaI AGT'ACT
Cuts at: 0 71 1417 1725
Size: 71 1346 308
Fragments arranged by size:
1346 308 71
SfcI C'TryA_G
Cuts at: 0 798 897 1175 1485 1725
Size: 798 99 278 310 240
Fragments arranged by size:
798 310 278 240 99
SfoI GGC'GCC
Cuts at: 0 1462 1725
Size: 1462 263
SgrAI Cr'CCGG_yG
Cuts at: 0 1457 1725
Size: 1457 268
SmaI CCC'GGG
Cuts at: 0 169 314 1725
Size: 169 145 1411
Fragments arranged by size:
1411 169 145
SmlI C'TyrA_G
Cuts at: 0 667 732 856 1725
Size: 667 65 124 869
Fragments arranged by size:
869 667 124 65
SrfI GCCC'GGGC
Cuts at: 0 169 1725
Size: 169 1556
StuI AGG'CCT
Cuts at: 0 522 895 1099 1213 1691 1725
Size: 522 373 204 114 478 34
Fragments arranged by size:
522 478 373 204 114 34
StyI C'CwwG_G
Cuts at: 0 85 839 1565 1620 1725
Size: 85 754 726 55 105
Fragments arranged by size:
754 726 105 85 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 484 577 1316 1633 1725
Size: 484 93 739 317 92
Fragments arranged by size:
739 484 317 93 92
TatI w'GTAC_w
Cuts at: 0 69 1415 1725
Size: 69 1346 310
Fragments arranged by size:
1346 310 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 1396 1551 1725
Size: 411 985 155 174
Fragments arranged by size:
985 411 174 155
XmaI C'CCGG_G
Cuts at: 0 167 312 1725
Size: 167 145 1413
Fragments arranged by size:
1413 167 145
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApaI ApaLI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BstYI BtgI BtsI CspCI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PpiI PspOMI PstI PvuII SacI SalI SapI ScaI
SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI BcgI BclI BglII
BmtI BsaI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI
EcoNI EcoRI EcoRV FseI HindIII HpaI MfeI MluI
NdeI NheI NotI NruI PacI PciI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SanDI SbfI SexAI SfiI SgfI SnaBI SpeI SphI
SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI