Restriction map for
pF1KB3004
(Linear) (Six-base) MAPSORT of: KIBB3004.seq Check: 14 from: 1 to: 3150
KIBB3004.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 2852 3150
Size: 2852 298
AclI AA'CG_TT
Cuts at: 0 298 2439 3150
Size: 298 2141 711
Fragments arranged by size:
2141 711 298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 148 1271 1770 2123 2212 2515 3150
Size: 106 42 1123 499 353 89 303 635
Fragments arranged by size:
1123 635 499 353 303 106 89 42
AleI CACnn'nnGTG
Cuts at: 0 2163 3150
Size: 2163 987
AlwNI CAG_nnn'CTG
Cuts at: 0 2190 2805 3028 3150
Size: 2190 615 223 122
Fragments arranged by size:
2190 615 223 122
ApoI r'AATT_y
Cuts at: 0 194 1225 1241 1738 2105 2983 3150
Size: 194 1031 16 497 367 878 167
Fragments arranged by size:
1031 878 497 367 194 167 16
AvaI C'yCGr_G
Cuts at: 0 593 3150
Size: 593 2557
BanI G'GyrC_C
Cuts at: 0 2349 2504 3150
Size: 2349 155 646
Fragments arranged by size:
2349 646 155
BanII G_rGCy'C
Cuts at: 0 720 2011 3150
Size: 720 1291 1139
Fragments arranged by size:
1291 1139 720
BbeI G_GCGC'C
Cuts at: 0 2353 3150
Size: 2353 797
BbsI GAAGACnn'nnnn_
Cuts at: 0 647 1671 1823 2027 2092 2243 2904 3150
Size: 647 1024 152 204 65 151 661 246
Fragments arranged by size:
1024 661 647 246 204 152 151 65
BbvCI CC'TCA_GC
Cuts at: 0 2779 3150
Size: 2779 371
BciVI GTATCCnnnnn_n'
Cuts at: 0 1863 3150
Size: 1863 1287
BclI T'GATC_A
Cuts at: 0 1395 2457 3150
Size: 1395 1062 693
Fragments arranged by size:
1395 1062 693
BglII A'GATC_T
Cuts at: 0 1926 3150
Size: 1926 1224
BlpI GC'TnA_GC
Cuts at: 0 2005 3150
Size: 2005 1145
BmrI ACTGGGnnnn_n'
Cuts at: 0 1007 1565 3150
Size: 1007 558 1585
Fragments arranged by size:
1585 1007 558
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 760 3150
Size: 760 2390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1706 3150
Size: 70 1636 1444
Fragments arranged by size:
1636 1444 70
Bpu10I CC'TnA_GC
Cuts at: 0 1096 2779 3150
Size: 1096 1683 371
Fragments arranged by size:
1683 1096 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 643 730 1779 2410 2667 3150
Size: 254 389 87 1049 631 257 483
Fragments arranged by size:
1049 631 483 389 257 254 87
BsaBI GATnn'nnATC
Cuts at: 0 2456 3150
Size: 2456 694
BsaHI Gr'CG_yC
Cuts at: 0 2350 3150
Size: 2350 800
BsaWI w'CCGG_w
Cuts at: 0 1259 1917 3150
Size: 1259 658 1233
Fragments arranged by size:
1259 1233 658
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 652 1183 2034 2076 3150
Size: 652 531 851 42 1074
Fragments arranged by size:
1074 851 652 531 42
BseYI C'CCAG_C
Cuts at: 0 572 3150
Size: 572 2578
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1325 2779 3150
Size: 1025 300 1454 371
Fragments arranged by size:
1454 1025 371 300
BsiHKAI G_wGCw'C
Cuts at: 0 30 132 720 744 3150
Size: 30 102 588 24 2406
Fragments arranged by size:
2406 588 102 30 24
BsmI GAATG_Cn'
Cuts at: 0 3009 3150
Size: 3009 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1402 3150
Size: 1402 1748
Bsp1286I G_dGCh'C
Cuts at: 0 30 132 720 744 2011 3150
Size: 30 102 588 24 1267 1139
Fragments arranged by size:
1267 1139 588 102 30 24
BspEI T'CCGG_A
Cuts at: 0 1259 3150
Size: 1259 1891
BspHI T'CATG_A
Cuts at: 0 1866 3150
Size: 1866 1284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 938 3150
Size: 938 2212
BsrDI GCAATG_nn'
Cuts at: 0 402 3150
Size: 402 2748
BsrGI T'GTAC_A
Cuts at: 0 84 413 2796 3150
Size: 84 329 2383 354
Fragments arranged by size:
2383 354 329 84
BssSI C'ACGA_G
Cuts at: 0 968 1646 3150
Size: 968 678 1504
Fragments arranged by size:
1504 968 678
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 439 3150
Size: 48 391 2711
Fragments arranged by size:
2711 391 48
BstYI r'GATC_y
Cuts at: 0 1128 1638 1887 1926 2535 2801 2819 3150
Size: 1128 510 249 39 609 266 18 331
Fragments arranged by size:
1128 609 510 331 266 249 39 18
Bsu36I CC'TnA_GG
Cuts at: 0 2752 3150
Size: 2752 398
BtgI C'CryG_G
Cuts at: 0 880 2161 2404 2637 3150
Size: 880 1281 243 233 513
Fragments arranged by size:
1281 880 513 243 233
DraI TTT'AAA
Cuts at: 0 2444 3150
Size: 2444 706
EarI CTCTTCn'nnn_
Cuts at: 0 167 629 1028 1306 1334 1400 3150
Size: 167 462 399 278 28 66 1750
Fragments arranged by size:
1750 462 399 278 167 66 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 120 3150
Size: 120 3030
EcoICRI GAG'CTC
Cuts at: 0 718 3150
Size: 718 2432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 106 148 1271 1706 1770 2123 2212
Size: 70 36 42 1123 435 64 353 89
Cuts at: 2212 2515 3150
Size: 303 635
Fragments arranged by size:
1123 635 435 353 303 89 70 64
42 36
EcoO109I rG'GnC_Cy
Cuts at: 0 931 950 1567 2924 3150
Size: 931 19 617 1357 226
Fragments arranged by size:
1357 931 617 226 19
EcoRI G'AATT_C
Cuts at: 0 1225 3150
Size: 1225 1925
HaeII r_GCGC'y
Cuts at: 0 2353 3150
Size: 2353 797
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 707 739 1861 1893 2804 2836 3150
Size: 707 32 1122 32 911 32 314
Fragments arranged by size:
1122 911 707 314 32 32 32
HincII GTy'rAC
Cuts at: 0 296 2748 3122 3150
Size: 296 2452 374 28
Fragments arranged by size:
2452 374 296 28
HindIII A'AGCT_T
Cuts at: 0 2714 3150
Size: 2714 436
HpaI GTT'AAC
Cuts at: 0 3122 3150
Size: 3122 28
KasI G'GCGC_C
Cuts at: 0 2349 3150
Size: 2349 801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2237 2568 3150
Size: 2237 331 582
Fragments arranged by size:
2237 582 331
MslI CAynn'nnrTG
Cuts at: 0 2163 3150
Size: 2163 987
MspA1I CmG'CkG
Cuts at: 0 248 1003 2471 2763 2783 3150
Size: 248 755 1468 292 20 367
Fragments arranged by size:
1468 755 367 292 248 20
NarI GG'CG_CC
Cuts at: 0 2350 3150
Size: 2350 800
NcoI C'CATG_G
Cuts at: 0 880 2161 2404 2637 3150
Size: 880 1281 243 233 513
Fragments arranged by size:
1281 880 513 243 233
NdeI CA'TA_TG
Cuts at: 0 1026 3150
Size: 1026 2124
PasI CC'CwG_GG
Cuts at: 0 902 3150
Size: 902 2248
PfoI T'CCnGG_A
Cuts at: 0 2804 3150
Size: 2804 346
PpuMI rG'GwC_Cy
Cuts at: 0 950 1567 2924 3150
Size: 950 617 1357 226
Fragments arranged by size:
1357 950 617 226
PshAI GACnn'nnGTC
Cuts at: 0 567 2648 3150
Size: 567 2081 502
Fragments arranged by size:
2081 567 502
PsiI TTA'TAA
Cuts at: 0 2591 3150
Size: 2591 559
PspXI vC'TCGA_Gb
Cuts at: 0 593 3150
Size: 593 2557
PstI C_TGCA'G
Cuts at: 0 1638 2223 2367 3150
Size: 1638 585 144 783
Fragments arranged by size:
1638 783 585 144
PvuII CAG'CTG
Cuts at: 0 248 2471 2763 3150
Size: 248 2223 292 387
Fragments arranged by size:
2223 387 292 248
SacI G_AGCT'C
Cuts at: 0 720 3150
Size: 720 2430
SexAI A'CCwGG_T
Cuts at: 0 2846 3150
Size: 2846 304
SfcI C'TryA_G
Cuts at: 0 314 565 848 869 1634 1977 2219 2363
Size: 314 251 283 21 765 343 242 144
Cuts at: 2363 2739 2905 3150
Size: 376 166 245
Fragments arranged by size:
765 376 343 314 283 251 245 242
166 144 21
SfoI GGC'GCC
Cuts at: 0 2351 3150
Size: 2351 799
SmlI C'TyrA_G
Cuts at: 0 269 593 622 745 1758 2425 2646 3150
Size: 269 324 29 123 1013 667 221 504
Fragments arranged by size:
1013 667 504 324 269 221 123 29
SpeI A'CTAG_T
Cuts at: 0 3124 3150
Size: 3124 26
SspI AAT'ATT
Cuts at: 0 2949 3150
Size: 2949 201
StuI AGG'CCT
Cuts at: 0 966 3150
Size: 966 2184
StyI C'CwwG_G
Cuts at: 0 432 880 934 953 2161 2404 2637 2653
Size: 432 448 54 19 1208 243 233 16
Cuts at: 2653 3150
Size: 497
Fragments arranged by size:
1208 497 448 432 243 233 54 19
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2334 3150
Size: 2334 816
TatI w'GTAC_w
Cuts at: 0 84 413 2796 3150
Size: 84 329 2383 354
Fragments arranged by size:
2383 354 329 84
XbaI T'CTAG_A
Cuts at: 0 1929 3150
Size: 1929 1221
XhoI C'TCGA_G
Cuts at: 0 593 3150
Size: 593 2557
XmnI GAAnn'nnTTC
Cuts at: 0 533 1241 2983 3150
Size: 533 708 1742 167
Fragments arranged by size:
1742 708 533 167
Enzymes that do cut:
AccI AclI AcuI AleI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrGI BssSI BstXI BstYI Bsu36I
BtgI DraI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI
MspA1I NarI NcoI NdeI PasI PfoI PpuMI PshAI
PsiI PspXI PstI PvuII SacI SexAI SfcI SfoI
SmlI SpeI SspI StuI StyI TaqII TatI XbaI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BfrBI BglI Bme1580I BmgBI BmtI
BsaI BsaAI BsaXI BsiEI BsiWI BsrBI BsrFI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EcoNI EcoRV FalI FseI
FspI FspAI KpnI MfeI MluI MscI NaeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PspOMI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI TstI Tth111I XcmI
XmaI ZraI