Restriction map for
pF1KB3009
(Linear) (Six-base) MAPSORT of: KIBB3009.seq Check: 5713 from: 1 to: 1944
KIBB3009.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1089 1944
Size: 1089 855
Acc65I G'GTAC_C
Cuts at: 0 1652 1944
Size: 1652 292
AclI AA'CG_TT
Cuts at: 0 712 1944
Size: 712 1232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1463 1536 1620 1788 1872 1944
Size: 1463 73 84 168 84 72
Fragments arranged by size:
1463 168 84 84 73 72
AlwNI CAG_nnn'CTG
Cuts at: 0 97 222 600 705 1944
Size: 97 125 378 105 1239
Fragments arranged by size:
1239 378 125 105 97
ApoI r'AATT_y
Cuts at: 0 50 1040 1380 1944
Size: 50 990 340 564
Fragments arranged by size:
990 564 340 50
AvaI C'yCGr_G
Cuts at: 0 511 625 1944
Size: 511 114 1319
Fragments arranged by size:
1319 511 114
BamHI G'GATC_C
Cuts at: 0 489 1944
Size: 489 1455
BanI G'GyrC_C
Cuts at: 0 555 1325 1652 1944
Size: 555 770 327 292
Fragments arranged by size:
770 555 327 292
BanII G_rGCy'C
Cuts at: 0 544 593 658 1944
Size: 544 49 65 1286
Fragments arranged by size:
1286 544 65 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 812 1675 1944
Size: 812 863 269
Fragments arranged by size:
863 812 269
BbvCI CC'TCA_GC
Cuts at: 0 1682 1944
Size: 1682 262
BglII A'GATC_T
Cuts at: 0 546 857 993 1944
Size: 546 311 136 951
Fragments arranged by size:
951 546 311 136
Bme1580I G_kGCm'C
Cuts at: 0 558 626 1870 1944
Size: 558 68 1244 74
Fragments arranged by size:
1244 558 74 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 613 1856 1944
Size: 613 1243 88
Fragments arranged by size:
1243 613 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 205 672 1944
Size: 205 467 1272
Fragments arranged by size:
1272 467 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 577 680 1003 1037 1532 1944
Size: 317 260 103 323 34 495 412
Fragments arranged by size:
495 412 323 317 260 103 34
Bpu10I CC'TnA_GC
Cuts at: 0 1682 1944
Size: 1682 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1109 1770 1944
Size: 902 207 661 174
Fragments arranged by size:
902 661 207 174
BsaI GGTCTCn'nnnn_
Cuts at: 0 219 381 1384 1944
Size: 219 162 1003 560
Fragments arranged by size:
1003 560 219 162
BsaAI yAC'GTr
Cuts at: 0 1865 1944
Size: 1865 79
BsaWI w'CCGG_w
Cuts at: 0 1373 1664 1944
Size: 1373 291 280
Fragments arranged by size:
1373 291 280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 779 809 1944
Size: 454 30 295 30 1135
Fragments arranged by size:
1135 454 295 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 454 601 666 1944
Size: 358 96 147 65 1278
Fragments arranged by size:
1278 358 147 96 65
BseYI C'CCAG_C
Cuts at: 0 311 1944
Size: 311 1633
BsiHKAI G_wGCw'C
Cuts at: 0 593 658 1944
Size: 593 65 1286
Fragments arranged by size:
1286 593 65
BsmBI CGTCTCn'nnnn_
Cuts at: 0 436 1944
Size: 436 1508
Bsp1286I G_dGCh'C
Cuts at: 0 544 558 593 626 658 1870 1944
Size: 544 14 35 33 32 1212 74
Fragments arranged by size:
1212 544 74 35 33 32 14
BspHI T'CATG_A
Cuts at: 0 1188 1944
Size: 1188 756
BsrBI CCG'CTC
Cuts at: 0 460 1944
Size: 460 1484
BsrFI r'CCGG_y
Cuts at: 0 558 1944
Size: 558 1386
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1706 1944
Size: 1706 238
BstXI CCAn_nnnn'nTGG
Cuts at: 0 398 1944
Size: 398 1546
BstYI r'GATC_y
Cuts at: 0 30 251 489 546 857 993 1944
Size: 30 221 238 57 311 136 951
Fragments arranged by size:
951 311 238 221 136 57 30
Bsu36I CC'TnA_GG
Cuts at: 0 524 908 1246 1944
Size: 524 384 338 698
Fragments arranged by size:
698 524 384 338
BtgI C'CryG_G
Cuts at: 0 11 1944
Size: 11 1933
BtsI GCAGTG_nn'
Cuts at: 0 356 1944
Size: 356 1588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1773 1808 1944
Size: 1773 35 136
Fragments arranged by size:
1773 136 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1822 1944
Size: 1822 122
EaeI y'GGCC_r
Cuts at: 0 88 328 420 725 1944
Size: 88 240 92 305 1219
Fragments arranged by size:
1219 305 240 92 88
EarI CTCTTCn'nnn_
Cuts at: 0 38 578 686 872 968 1382 1792 1944
Size: 38 540 108 186 96 414 410 152
Fragments arranged by size:
540 414 410 186 152 108 96 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 1944
Size: 252 1692
EcoICRI GAG'CTC
Cuts at: 0 591 656 1944
Size: 591 65 1288
Fragments arranged by size:
1288 591 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 577 680 1003 1037 1463 1532 1536
Size: 317 260 103 323 34 426 69 4
Cuts at: 1536 1620 1788 1872 1944
Size: 84 168 84 72
Fragments arranged by size:
426 323 317 260 168 103 84 84
72 69 34 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 436 1767 1944
Size: 436 1331 177
Fragments arranged by size:
1331 436 177
EcoO109I rG'GnC_Cy
Cuts at: 0 615 630 888 1772 1922 1944
Size: 615 15 258 884 150 22
Fragments arranged by size:
884 615 258 150 22 15
EcoRI G'AATT_C
Cuts at: 0 50 1944
Size: 50 1894
EcoRV GAT'ATC
Cuts at: 0 897 1900 1944
Size: 897 1003 44
Fragments arranged by size:
1003 897 44
HaeII r_GCGC'y
Cuts at: 0 1716 1944
Size: 1716 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 531 563 663 695 779 811
Size: 454 32 45 32 100 32 84 32
Cuts at: 811 844 876 1791 1823 1944
Size: 33 32 915 32 121
Fragments arranged by size:
915 454 121 100 84 45 33 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1571 1944
Size: 1571 373
HindIII A'AGCT_T
Cuts at: 0 1548 1884 1944
Size: 1548 336 60
Fragments arranged by size:
1548 336 60
HpaI GTT'AAC
Cuts at: 0 1571 1944
Size: 1571 373
KpnI G_GTAC'C
Cuts at: 0 1656 1944
Size: 1656 288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1944
Size: 171 1773
MslI CAynn'nnrTG
Cuts at: 0 1863 1944
Size: 1863 81
MspA1I CmG'CkG
Cuts at: 0 1472 1944
Size: 1472 472
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 1944
Size: 337 1607
PmlI CAC'GTG
Cuts at: 0 1865 1944
Size: 1865 79
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1679 1711 1944
Size: 1679 32 233
Fragments arranged by size:
1679 233 32
PpuMI rG'GwC_Cy
Cuts at: 0 615 630 888 1922 1944
Size: 615 15 258 1034 22
Fragments arranged by size:
1034 615 258 22 15
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1339 1371 1944
Size: 1339 32 573
Fragments arranged by size:
1339 573 32
PstI C_TGCA'G
Cuts at: 0 488 605 648 1702 1944
Size: 488 117 43 1054 242
Fragments arranged by size:
1054 488 242 117 43
SacI G_AGCT'C
Cuts at: 0 593 658 1944
Size: 593 65 1286
Fragments arranged by size:
1286 593 65
SanDI GG'GwC_CC
Cuts at: 0 888 1944
Size: 888 1056
SapI GCTCTTCn'nnn_
Cuts at: 0 686 872 1792 1944
Size: 686 186 920 152
Fragments arranged by size:
920 686 186 152
SbfI CC_TGCA'GG
Cuts at: 0 605 1944
Size: 605 1339
SfcI C'TryA_G
Cuts at: 0 5 304 484 601 644 1440 1698 1944
Size: 5 299 180 117 43 796 258 246
Fragments arranged by size:
796 299 258 246 180 117 43 5
SmlI C'TyrA_G
Cuts at: 0 917 1124 1749 1944
Size: 917 207 625 195
Fragments arranged by size:
917 625 207 195
SspI AAT'ATT
Cuts at: 0 1119 1944
Size: 1119 825
StuI AGG'CCT
Cuts at: 0 1020 1944
Size: 1020 924
StyI C'CwwG_G
Cuts at: 0 391 883 1944
Size: 391 492 1061
Fragments arranged by size:
1061 492 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 390 1738 1944
Size: 303 87 1348 206
Fragments arranged by size:
1348 303 206 87
TatI w'GTAC_w
Cuts at: 0 1531 1944
Size: 1531 413
Tth111I GACn'n_nGTC
Cuts at: 0 1486 1944
Size: 1486 458
XmnI GAAnn'nnTTC
Cuts at: 0 278 778 1801 1858 1944
Size: 278 500 1023 57 86
Fragments arranged by size:
1023 500 278 86 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII HindIII HpaI KpnI MmeI MslI
MspA1I PflMI PmlI PpiI PpuMI PsrI PstI SacI
SanDI SapI SbfI SfcI SmlI SspI StuI StyI
TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BsaBI BsaHI BsgI BsiEI BsiWI BsmI
BspEI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI DraI DraIII EagI FalI FseI
FspI FspAI KasI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PshAI PsiI PspOMI
PspXI PvuI PvuII RsrII SacII SalI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TstI XbaI XcmI XhoI XmaI ZraI