Restriction map for
pF1KB3011
(Linear) (Six-base) MAPSORT of: KIBB3011.seq Check: 6737 from: 1 to: 2163
KIBB3011.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 688 2163
Size: 688 1475
AccI GT'mk_AC
Cuts at: 0 113 2163
Size: 113 2050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 587 2163
Size: 520 67 1576
Fragments arranged by size:
1576 520 67
AfeI AGC'GCT
Cuts at: 0 1517 2163
Size: 1517 646
AflII C'TTAA_G
Cuts at: 0 2068 2163
Size: 2068 95
AflIII A'CryG_T
Cuts at: 0 641 2163
Size: 641 1522
AlwNI CAG_nnn'CTG
Cuts at: 0 1539 2163
Size: 1539 624
ApaI G_GGCC'C
Cuts at: 0 367 2163
Size: 367 1796
ApaLI G'TGCA_C
Cuts at: 0 701 2163
Size: 701 1462
ApoI r'AATT_y
Cuts at: 0 10 884 2120 2163
Size: 10 874 1236 43
Fragments arranged by size:
1236 874 43 10
AseI AT'TA_AT
Cuts at: 0 479 2163
Size: 479 1684
AvaI C'yCGr_G
Cuts at: 0 2020 2163
Size: 2020 143
BamHI G'GATC_C
Cuts at: 0 534 1233 2163
Size: 534 699 930
Fragments arranged by size:
930 699 534
BanII G_rGCy'C
Cuts at: 0 289 367 419 1152 2163
Size: 289 78 52 733 1011
Fragments arranged by size:
1011 733 289 78 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 494 1763 2163
Size: 216 278 1269 400
Fragments arranged by size:
1269 400 278 216
BglI GCCn_nnn'nGGC
Cuts at: 0 1272 2163
Size: 1272 891
BglII A'GATC_T
Cuts at: 0 453 592 1422 2163
Size: 453 139 830 741
Fragments arranged by size:
830 741 453 139
Bme1580I G_kGCm'C
Cuts at: 0 367 705 2163
Size: 367 338 1458
Fragments arranged by size:
1458 367 338
BmgBI CAC'GTC
Cuts at: 0 601 2163
Size: 601 1562
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 1068 2163
Size: 251 817 1095
Fragments arranged by size:
1095 817 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1249 1333 2163
Size: 376 873 84 830
Fragments arranged by size:
873 830 376 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 2163
Size: 403 1760
BsaAI yAC'GTr
Cuts at: 0 190 2163
Size: 190 1973
BsaBI GATnn'nnATC
Cuts at: 0 1211 2163
Size: 1211 952
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1368 1398 2163
Size: 1368 30 765
Fragments arranged by size:
1368 765 30
BseYI C'CCAG_C
Cuts at: 0 1616 2163
Size: 1616 547
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 2163
Size: 430 1733
BsiHKAI G_wGCw'C
Cuts at: 0 705 1152 2163
Size: 705 447 1011
Fragments arranged by size:
1011 705 447
BsmI GAATG_Cn'
Cuts at: 0 2144 2163
Size: 2144 19
Bsp1286I G_dGCh'C
Cuts at: 0 289 367 419 705 1152 2163
Size: 289 78 52 286 447 1011
Fragments arranged by size:
1011 447 289 286 78 52
BspHI T'CATG_A
Cuts at: 0 23 1071 1290 2163
Size: 23 1048 219 873
Fragments arranged by size:
1048 873 219 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 688 2163
Size: 688 1475
BsrDI GCAATG_nn'
Cuts at: 0 96 2163
Size: 96 2067
BsrGI T'GTAC_A
Cuts at: 0 750 1104 2163
Size: 750 354 1059
Fragments arranged by size:
1059 750 354
BstEII G'GTnAC_C
Cuts at: 0 2060 2163
Size: 2060 103
BstYI r'GATC_y
Cuts at: 0 453 534 592 1233 1422 2163
Size: 453 81 58 641 189 741
Fragments arranged by size:
741 641 453 189 81 58
BtgI C'CryG_G
Cuts at: 0 902 2163
Size: 902 1261
EarI CTCTTCn'nnn_
Cuts at: 0 371 2163
Size: 371 1792
EcoICRI GAG'CTC
Cuts at: 0 1150 2163
Size: 1150 1013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 520 587 1249 1333 2163
Size: 376 144 67 662 84 830
Fragments arranged by size:
830 662 376 144 84 67
EcoO109I rG'GnC_Cy
Cuts at: 0 363 364 2163
Size: 363 1 1799
Fragments arranged by size:
1799 363 1
EcoRV GAT'ATC
Cuts at: 0 1697 2163
Size: 1697 466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1007 1153 2163
Size: 1007 146 1010
Fragments arranged by size:
1010 1007 146
HaeII r_GCGC'y
Cuts at: 0 1519 2163
Size: 1519 644
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 125 157 237 269 271 303 519 551
Size: 125 32 80 32 2 32 216 32
Cuts at: 551 1513 1545 2163
Size: 962 32 618
Fragments arranged by size:
962 618 216 125 80 32 32 32
32 32 2
HincII GTy'rAC
Cuts at: 0 914 1392 2163
Size: 914 478 771
Fragments arranged by size:
914 771 478
HindIII A'AGCT_T
Cuts at: 0 1411 1951 2163
Size: 1411 540 212
Fragments arranged by size:
1411 540 212
HpaI GTT'AAC
Cuts at: 0 914 1392 2163
Size: 914 478 771
Fragments arranged by size:
914 771 478
MfeI C'AATT_G
Cuts at: 0 1431 2163
Size: 1431 732
MslI CAynn'nnrTG
Cuts at: 0 640 1400 2163
Size: 640 760 763
Fragments arranged by size:
763 760 640
MspA1I CmG'CkG
Cuts at: 0 405 726 840 2163
Size: 405 321 114 1323
Fragments arranged by size:
1323 405 321 114
NcoI C'CATG_G
Cuts at: 0 902 2163
Size: 902 1261
NspI r_CATG'y
Cuts at: 0 184 385 645 2163
Size: 184 201 260 1518
Fragments arranged by size:
1518 260 201 184
PciI A'CATG_T
Cuts at: 0 641 2163
Size: 641 1522
PfoI T'CCnGG_A
Cuts at: 0 846 875 2163
Size: 846 29 1288
Fragments arranged by size:
1288 846 29
PshAI GACnn'nnGTC
Cuts at: 0 515 2163
Size: 515 1648
PsiI TTA'TAA
Cuts at: 0 1055 2163
Size: 1055 1108
PspOMI G'GGCC_C
Cuts at: 0 363 2163
Size: 363 1800
PspXI vC'TCGA_Gb
Cuts at: 0 2020 2163
Size: 2020 143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 295 327 2163
Size: 295 32 1836
Fragments arranged by size:
1836 295 32
PstI C_TGCA'G
Cuts at: 0 654 2163
Size: 654 1509
PvuII CAG'CTG
Cuts at: 0 405 726 840 2163
Size: 405 321 114 1323
Fragments arranged by size:
1323 405 321 114
SacI G_AGCT'C
Cuts at: 0 1152 2163
Size: 1152 1011
SapI GCTCTTCn'nnn_
Cuts at: 0 371 2163
Size: 371 1792
SfcI C'TryA_G
Cuts at: 0 110 650 2163
Size: 110 540 1513
Fragments arranged by size:
1513 540 110
SmlI C'TyrA_G
Cuts at: 0 418 2020 2068 2163
Size: 418 1602 48 95
Fragments arranged by size:
1602 418 95 48
SpeI A'CTAG_T
Cuts at: 0 1876 2163
Size: 1876 287
SphI G_CATG'C
Cuts at: 0 385 2163
Size: 385 1778
SspI AAT'ATT
Cuts at: 0 1201 1303 2163
Size: 1201 102 860
Fragments arranged by size:
1201 860 102
StyI C'CwwG_G
Cuts at: 0 902 2163
Size: 902 1261
TatI w'GTAC_w
Cuts at: 0 326 441 750 1104 1179 2163
Size: 326 115 309 354 75 984
Fragments arranged by size:
984 354 326 309 115 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 857 889 1368 1400 2163
Size: 857 32 479 32 763
Fragments arranged by size:
857 763 479 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1792 2163
Size: 1792 371
XhoI C'TCGA_G
Cuts at: 0 2020 2163
Size: 2020 143
XmnI GAAnn'nnTTC
Cuts at: 0 740 1979 2163
Size: 740 1239 184
Fragments arranged by size:
1239 740 184
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AlwNI ApaI
ApaLI ApoI AseI AvaI BamHI BanII BbsI BglI
BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaBI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstEII BstYI BtgI EarI EcoICRI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MfeI MslI MspA1I NcoI NspI PciI PfoI PshAI
PsiI PspOMI PspXI PsrI PstI PvuII SacI SapI
SfcI SmlI SpeI SphI SspI StyI TatI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AleI AlfI AloI
AscI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BlpI BmtI BplI Bpu10I BsaI
BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoRI FseI FspI FspAI KasI
KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI StuI SwaI TaqII Tth111I XbaI XmaI
ZraI