Restriction map for
pF1KB3013
(Linear) (Six-base) MAPSORT of: KIBB3013.seq Check: 2529 from: 1 to: 1368
KIBB3013.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 765 1217 1368
Size: 765 452 151
Fragments arranged by size:
765 452 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1368
Size: 820 548
AlwNI CAG_nnn'CTG
Cuts at: 0 798 1143 1293 1368
Size: 798 345 150 75
Fragments arranged by size:
798 345 150 75
ApoI r'AATT_y
Cuts at: 0 550 634 893 1368
Size: 550 84 259 475
Fragments arranged by size:
550 475 259 84
AseI AT'TA_AT
Cuts at: 0 370 1303 1368
Size: 370 933 65
Fragments arranged by size:
933 370 65
AvaI C'yCGr_G
Cuts at: 0 85 1368
Size: 85 1283
BamHI G'GATC_C
Cuts at: 0 712 1257 1368
Size: 712 545 111
Fragments arranged by size:
712 545 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 1368
Size: 120 1248
BmtI G_CTAG'C
Cuts at: 0 103 1368
Size: 103 1265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 504 1368
Size: 504 864
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1368
Size: 1018 350
BsaI GGTCTCn'nnnn_
Cuts at: 0 1242 1289 1368
Size: 1242 47 79
Fragments arranged by size:
1242 79 47
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1360 1368
Size: 1360 8
BsrBI CCG'CTC
Cuts at: 0 21 1368
Size: 21 1347
BstBI TT'CG_AA
Cuts at: 0 600 1368
Size: 600 768
BstEII G'GTnAC_C
Cuts at: 0 390 1368
Size: 390 978
BstYI r'GATC_y
Cuts at: 0 712 909 1146 1257 1368
Size: 712 197 237 111 111
Fragments arranged by size:
712 237 197 111 111
BstZ17I GTA'TAC
Cuts at: 0 766 1368
Size: 766 602
BtgI C'CryG_G
Cuts at: 0 65 1368
Size: 65 1303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 942 977 1368
Size: 942 35 391
Fragments arranged by size:
942 391 35
DraI TTT'AAA
Cuts at: 0 917 1368
Size: 917 451
EaeI y'GGCC_r
Cuts at: 0 1097 1368
Size: 1097 271
EarI CTCTTCn'nnn_
Cuts at: 0 796 1368
Size: 796 572
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 294 1368
Size: 19 275 1074
Fragments arranged by size:
1074 275 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1368
Size: 820 548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 133 1012 1221 1368
Size: 133 879 209 147
Fragments arranged by size:
879 209 147 133
HaeII r_GCGC'y
Cuts at: 0 65 81 1368
Size: 65 16 1287
Fragments arranged by size:
1287 65 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 166 198 1344 1368
Size: 30 136 32 1146 24
Fragments arranged by size:
1146 136 32 30 24
HincII GTy'rAC
Cuts at: 0 1194 1317 1368
Size: 1194 123 51
Fragments arranged by size:
1194 123 51
HindIII A'AGCT_T
Cuts at: 0 919 1368
Size: 919 449
HpaI GTT'AAC
Cuts at: 0 1194 1317 1368
Size: 1194 123 51
Fragments arranged by size:
1194 123 51
MscI TGG'CCA
Cuts at: 0 1099 1368
Size: 1099 269
MspA1I CmG'CkG
Cuts at: 0 696 1368
Size: 696 672
NheI G'CTAG_C
Cuts at: 0 99 1368
Size: 99 1269
PfoI T'CCnGG_A
Cuts at: 0 641 1368
Size: 641 727
PvuII CAG'CTG
Cuts at: 0 696 1368
Size: 696 672
ScaI AGT'ACT
Cuts at: 0 676 1368
Size: 676 692
SfcI C'TryA_G
Cuts at: 0 57 738 1028 1368
Size: 57 681 290 340
Fragments arranged by size:
681 340 290 57
SmlI C'TyrA_G
Cuts at: 0 997 1368
Size: 997 371
SspI AAT'ATT
Cuts at: 0 258 1086 1368
Size: 258 828 282
Fragments arranged by size:
828 282 258
StyI C'CwwG_G
Cuts at: 0 104 1253 1368
Size: 104 1149 115
Fragments arranged by size:
1149 115 104
TatI w'GTAC_w
Cuts at: 0 325 674 810 1368
Size: 325 349 136 558
Fragments arranged by size:
558 349 325 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 336 368 1368
Size: 336 32 1000
Fragments arranged by size:
1000 336 32
XmnI GAAnn'nnTTC
Cuts at: 0 137 893 990 1172 1368
Size: 137 756 97 182 196
Fragments arranged by size:
756 196 182 137 97
Enzymes that do cut:
AccI AcuI AlwNI ApoI AseI AvaI BamHI BbsI
BmtI BplI BpuEI BsaI BseRI BsrBI BstBI BstEII
BstYI BstZ17I BtgI CspCI DraI EaeI EarI EciI
Eco57MI FalI HaeII Hin4I HincII HindIII HpaI MscI
MspA1I NheI PfoI PvuII ScaI SfcI SmlI SspI
StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstXI Bsu36I BtgZI BtsI ClaI DraIII DrdI EagI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI