Restriction map for
pF1KB3015
(Linear) (Six-base) MAPSORT of: KIBB3015.seq Check: 6705 from: 1 to: 2208
KIBB3015.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 810 1260 2208
Size: 810 450 948
Fragments arranged by size:
948 810 450
AatII G_ACGT'C
Cuts at: 0 1067 2208
Size: 1067 1141
AccI GT'mk_AC
Cuts at: 0 1743 2208
Size: 1743 465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1427 2208
Size: 884 543 781
Fragments arranged by size:
884 781 543
AfeI AGC'GCT
Cuts at: 0 1717 2208
Size: 1717 491
AgeI A'CCGG_T
Cuts at: 0 143 2208
Size: 143 2065
AleI CACnn'nnGTG
Cuts at: 0 1030 2208
Size: 1030 1178
AlwNI CAG_nnn'CTG
Cuts at: 0 375 2208
Size: 375 1833
ApoI r'AATT_y
Cuts at: 0 379 514 685 1005 2208
Size: 379 135 171 320 1203
Fragments arranged by size:
1203 379 320 171 135
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1049 1082 2208
Size: 1049 33 1126
Fragments arranged by size:
1126 1049 33
BanI G'GyrC_C
Cuts at: 0 1120 2208
Size: 1120 1088
BanII G_rGCy'C
Cuts at: 0 1336 2208
Size: 1336 872
BciVI GTATCCnnnnn_n'
Cuts at: 0 1681 2208
Size: 1681 527
BglII A'GATC_T
Cuts at: 0 1280 2208
Size: 1280 928
Bme1580I G_kGCm'C
Cuts at: 0 898 1123 2208
Size: 898 225 1085
Fragments arranged by size:
1085 898 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 731 2208
Size: 731 1477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1224 1637 2012 2208
Size: 1224 413 375 196
Fragments arranged by size:
1224 413 375 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 2208
Size: 165 2043
BsaHI Gr'CG_yC
Cuts at: 0 1064 2208
Size: 1064 1144
BsaWI w'CCGG_w
Cuts at: 0 143 2208
Size: 143 2065
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1212 1242 1780 1810 2208
Size: 1212 30 538 30 398
Fragments arranged by size:
1212 538 398 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 533 581 809 2208
Size: 533 48 228 1399
Fragments arranged by size:
1399 533 228 48
BseYI C'CCAG_C
Cuts at: 0 1460 2093 2125 2208
Size: 1460 633 32 83
Fragments arranged by size:
1460 633 83 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1694 2208
Size: 1694 514
BsmI GAATG_Cn'
Cuts at: 0 421 790 2208
Size: 421 369 1418
Fragments arranged by size:
1418 421 369
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1496 1507 2208
Size: 1496 11 701
Fragments arranged by size:
1496 701 11
Bsp1286I G_dGCh'C
Cuts at: 0 898 1123 1336 2208
Size: 898 225 213 872
Fragments arranged by size:
898 872 225 213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 551 810 1260 2208
Size: 551 259 450 948
Fragments arranged by size:
948 551 450 259
BsrDI GCAATG_nn'
Cuts at: 0 860 2208
Size: 860 1348
BsrFI r'CCGG_y
Cuts at: 0 143 2021 2208
Size: 143 1878 187
Fragments arranged by size:
1878 187 143
BsrGI T'GTAC_A
Cuts at: 0 342 2208
Size: 342 1866
BstBI TT'CG_AA
Cuts at: 0 1683 2208
Size: 1683 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 202 2208
Size: 117 85 2006
Fragments arranged by size:
2006 117 85
BstYI r'GATC_y
Cuts at: 0 489 879 1280 2159 2208
Size: 489 390 401 879 49
Fragments arranged by size:
879 489 401 390 49
BstZ17I GTA'TAC
Cuts at: 0 1744 2208
Size: 1744 464
BtgI C'CryG_G
Cuts at: 0 1306 2208
Size: 1306 902
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 2208
Size: 116 2092
BtsI GCAGTG_nn'
Cuts at: 0 275 398 2039 2208
Size: 275 123 1641 169
Fragments arranged by size:
1641 275 169 123
DrdI GACnn_nn'nnGTC
Cuts at: 0 621 2208
Size: 621 1587
EaeI y'GGCC_r
Cuts at: 0 1259 2208
Size: 1259 949
EarI CTCTTCn'nnn_
Cuts at: 0 224 227 2208
Size: 224 3 1981
Fragments arranged by size:
1981 224 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1224 1427 1637 2012 2208
Size: 884 340 203 210 375 196
Fragments arranged by size:
884 375 340 210 203 196
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1052 2208
Size: 1052 1156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1809 2208
Size: 1809 399
HaeII r_GCGC'y
Cuts at: 0 1719 2208
Size: 1719 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 1609 1641 2208
Size: 1522 32 55 32 567
Fragments arranged by size:
1522 567 55 32 32
HincII GTy'rAC
Cuts at: 0 1037 1377 1823 2208
Size: 1037 340 446 385
Fragments arranged by size:
1037 446 385 340
HindIII A'AGCT_T
Cuts at: 0 1645 2208
Size: 1645 563
HpaI GTT'AAC
Cuts at: 0 1037 2208
Size: 1037 1171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 2208
Size: 1136 1072
MscI TGG'CCA
Cuts at: 0 1261 2208
Size: 1261 947
MslI CAynn'nnrTG
Cuts at: 0 115 983 1030 2208
Size: 115 868 47 1178
Fragments arranged by size:
1178 868 115 47
MspA1I CmG'CkG
Cuts at: 0 81 129 1662 1927 2208
Size: 81 48 1533 265 281
Fragments arranged by size:
1533 281 265 81 48
NdeI CA'TA_TG
Cuts at: 0 618 984 1479 2208
Size: 618 366 495 729
Fragments arranged by size:
729 618 495 366
NspI r_CATG'y
Cuts at: 0 164 2208
Size: 164 2044
PfoI T'CCnGG_A
Cuts at: 0 800 2208
Size: 800 1408
PsiI TTA'TAA
Cuts at: 0 1390 2208
Size: 1390 818
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1979 2011 2208
Size: 1979 32 197
Fragments arranged by size:
1979 197 32
PstI C_TGCA'G
Cuts at: 0 405 1932 2208
Size: 405 1527 276
Fragments arranged by size:
1527 405 276
PvuII CAG'CTG
Cuts at: 0 1662 1927 2208
Size: 1662 265 281
Fragments arranged by size:
1662 281 265
RsrII CG'GwC_CG
Cuts at: 0 146 2208
Size: 146 2062
ScaI AGT'ACT
Cuts at: 0 1395 1404 1921 1974 2208
Size: 1395 9 517 53 234
Fragments arranged by size:
1395 517 234 53 9
SexAI A'CCwGG_T
Cuts at: 0 1341 2208
Size: 1341 867
SfcI C'TryA_G
Cuts at: 0 401 1928 2208
Size: 401 1527 280
Fragments arranged by size:
1527 401 280
SmlI C'TyrA_G
Cuts at: 0 180 2208
Size: 180 2028
SphI G_CATG'C
Cuts at: 0 164 2208
Size: 164 2044
SspI AAT'ATT
Cuts at: 0 351 2208
Size: 351 1857
TatI w'GTAC_w
Cuts at: 0 342 1393 1402 1919 1972 2194 2208
Size: 342 1051 9 517 53 222 14
Fragments arranged by size:
1051 517 342 222 53 14 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 922 954 1212 1244 2208
Size: 301 32 589 32 258 32 964
Fragments arranged by size:
964 589 301 258 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 712 2208
Size: 712 1496
XbaI T'CTAG_A
Cuts at: 0 492 2208
Size: 492 1716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 697 2208
Size: 697 1511
ZraI GAC'GTC
Cuts at: 0 1065 2208
Size: 1065 1143
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AgeI AleI AlwNI
ApoI BaeI BanI BanII BciVI BglII Bme1580I BmrI
BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DrdI EaeI
EarI Eco57MI EcoNI FalI HaeII Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NdeI NspI PfoI
PsiI PsrI PstI PvuII RsrII ScaI SexAI SfcI
SmlI SphI SspI TatI TstI Tth111I XbaI XcmI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiEI BsiHKAI
BsiWI BspEI BspHI BsrBI BssHII BssSI BstAPI BstEII
Bsu36I ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FseI FspI FspAI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PvuI SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI StyI SwaI TaqII
XhoI XmaI XmnI