Restriction map for
pF1KB3017
(Linear) (Six-base) MAPSORT of: KIBB3017.seq Check: 707 from: 1 to: 2394
KIBB3017.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AccI GT'mk_AC
Cuts at: 0 1064 2394
Size: 1064 1330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 845 1953 2394
Size: 794 51 1108 441
Fragments arranged by size:
1108 794 441 51
AfeI AGC'GCT
Cuts at: 0 664 2394
Size: 664 1730
AflIII A'CryG_T
Cuts at: 0 507 2394
Size: 507 1887
AleI CACnn'nnGTG
Cuts at: 0 1463 2128 2394
Size: 1463 665 266
Fragments arranged by size:
1463 665 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 1806 2146 2394
Size: 928 878 340 248
Fragments arranged by size:
928 878 340 248
AlwNI CAG_nnn'CTG
Cuts at: 0 1074 2394
Size: 1074 1320
ApaI G_GGCC'C
Cuts at: 0 1377 1604 2394
Size: 1377 227 790
Fragments arranged by size:
1377 790 227
AvaI C'yCGr_G
Cuts at: 0 263 520 1822 2394
Size: 263 257 1302 572
Fragments arranged by size:
1302 572 263 257
AvrII C'CTAG_G
Cuts at: 0 1518 2394
Size: 1518 876
BanI G'GyrC_C
Cuts at: 0 340 355 369 740 1577 2394
Size: 340 15 14 371 837 817
Fragments arranged by size:
837 817 371 340 15 14
BanII G_rGCy'C
Cuts at: 0 296 410 1355 1377 1587 1604 1663 1835
Size: 296 114 945 22 210 17 59 172
Cuts at: 1835 2394
Size: 559
Fragments arranged by size:
945 559 296 210 172 114 59 22
17
BbeI G_GCGC'C
Cuts at: 0 344 373 744 2394
Size: 344 29 371 1650
Fragments arranged by size:
1650 371 344 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1160 2394
Size: 1160 1234
BbvCI CC'TCA_GC
Cuts at: 0 440 1265 2394
Size: 440 825 1129
Fragments arranged by size:
1129 825 440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1262 1296 2394
Size: 1262 34 1098
Fragments arranged by size:
1262 1098 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 439 2394
Size: 439 1955
BlpI GC'TnA_GC
Cuts at: 0 331 2394
Size: 331 2063
Bme1580I G_kGCm'C
Cuts at: 0 527 929 1377 1509 1604 2013 2394
Size: 527 402 448 132 95 409 381
Fragments arranged by size:
527 448 409 402 381 132 95
BmgBI CAC'GTC
Cuts at: 0 1290 2394
Size: 1290 1104
BmrI ACTGGGnnnn_n'
Cuts at: 0 911 1224 2394
Size: 911 313 1170
Fragments arranged by size:
1170 911 313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1572 2394
Size: 1572 822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 307 403 679 849 1107 2394
Size: 298 9 96 276 170 258 1287
Fragments arranged by size:
1287 298 276 258 170 96 9
Bpu10I CC'TnA_GC
Cuts at: 0 40 440 478 1265 2394
Size: 40 400 38 787 1129
Fragments arranged by size:
1129 787 400 40 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 2136 2394
Size: 2136 258
BsaHI Gr'CG_yC
Cuts at: 0 65 341 370 741 2114 2394
Size: 65 276 29 371 1373 280
Fragments arranged by size:
1373 371 280 276 65 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 295 304 1405 2394
Size: 265 30 9 1101 989
Fragments arranged by size:
1101 989 265 30 9
BseYI C'CCAG_C
Cuts at: 0 123 175 362 410 883 1442 2184 2217
Size: 123 52 187 48 473 559 742 33
Cuts at: 2217 2394
Size: 177
Fragments arranged by size:
742 559 473 187 177 123 52 48
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1629 1973 2394
Size: 788 841 344 421
Fragments arranged by size:
841 788 421 344
BsiEI CG_ry'CG
Cuts at: 0 2129 2394
Size: 2129 265
BsiHKAI G_wGCw'C
Cuts at: 0 619 1540 1663 1899 2135 2394
Size: 619 921 123 236 236 259
Fragments arranged by size:
921 619 259 236 236 123
BsmI GAATG_Cn'
Cuts at: 0 1795 2394
Size: 1795 599
Bsp1286I G_dGCh'C
Cuts at: 0 296 410 527 619 929 1355 1377 1509
Size: 296 114 117 92 310 426 22 132
Cuts at: 1509 1540 1587 1604 1663 1835 1899 2013 2135
Size: 31 47 17 59 172 64 114 122
Cuts at: 2135 2394
Size: 259
Fragments arranged by size:
426 310 296 259 172 132 122 117
114 114 92 64 59 47 31 22
17
BsrBI CCG'CTC
Cuts at: 0 330 729 2070 2394
Size: 330 399 1341 324
Fragments arranged by size:
1341 399 330 324
BsrDI GCAATG_nn'
Cuts at: 0 951 2394
Size: 951 1443
BsrFI r'CCGG_y
Cuts at: 0 2237 2394
Size: 2237 157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 312 531 1718 1784 1868 2394
Size: 312 219 1187 66 84 526
Fragments arranged by size:
1187 526 312 219 84 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 366 657 883 1131 2184 2394
Size: 366 291 226 248 1053 210
Fragments arranged by size:
1053 366 291 248 226 210
BstYI r'GATC_y
Cuts at: 0 71 2394
Size: 71 2323
Bsu36I CC'TnA_GG
Cuts at: 0 1363 2394
Size: 1363 1031
BtgI C'CryG_G
Cuts at: 0 297 345 2394
Size: 297 48 2049
Fragments arranged by size:
2049 297 48
BtsI GCAGTG_nn'
Cuts at: 0 927 1054 1338 1440 1458 1470 1617 1656
Size: 927 127 284 102 18 12 147 39
Cuts at: 1656 1800 1845 2216 2394
Size: 144 45 371 178
Fragments arranged by size:
927 371 284 178 147 144 127 102
45 39 18 12
DraIII CAC_nnn'GTG
Cuts at: 0 693 1418 2394
Size: 693 725 976
Fragments arranged by size:
976 725 693
EaeI y'GGCC_r
Cuts at: 0 300 1470 1512 1985 2394
Size: 300 1170 42 473 409
Fragments arranged by size:
1170 473 409 300 42
EarI CTCTTCn'nnn_
Cuts at: 0 1197 1243 2394
Size: 1197 46 1151
Fragments arranged by size:
1197 1151 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 117 238 2394
Size: 117 121 2156
Fragments arranged by size:
2156 121 117
EcoICRI GAG'CTC
Cuts at: 0 1661 2394
Size: 1661 733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 307 403 679 794 845 849 1107
Size: 298 9 96 276 115 51 4 258
Cuts at: 1107 1953 2394
Size: 846 441
Fragments arranged by size:
846 441 298 276 258 115 96 51
9 4
EcoO109I rG'GnC_Cy
Cuts at: 0 128 1005 1240 1374 1600 1601 1964 2394
Size: 128 877 235 134 226 1 363 430
Fragments arranged by size:
877 430 363 235 226 134 128 1
FspI TGC'GCA
Cuts at: 0 568 2394
Size: 568 1826
FspAI rTGC'GCAy
Cuts at: 0 568 2394
Size: 568 1826
HaeII r_GCGC'y
Cuts at: 0 237 344 373 666 744 2394
Size: 237 107 29 293 78 1650
Fragments arranged by size:
1650 293 237 107 78 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1555 1587 2111 2143 2394
Size: 1555 32 524 32 251
Fragments arranged by size:
1555 524 251 32 32
KasI G'GCGC_C
Cuts at: 0 340 369 740 2394
Size: 340 29 371 1654
Fragments arranged by size:
1654 371 340 29
MluI A'CGCG_T
Cuts at: 0 507 2394
Size: 507 1887
MscI TGG'CCA
Cuts at: 0 1472 1987 2394
Size: 1472 515 407
Fragments arranged by size:
1472 515 407
MslI CAynn'nnrTG
Cuts at: 0 910 1463 1760 1788 2018 2128 2394
Size: 910 553 297 28 230 110 266
Fragments arranged by size:
910 553 297 266 230 110 28
MspA1I CmG'CkG
Cuts at: 0 179 276 299 347 366 378 883 1502
Size: 179 97 23 48 19 12 505 619
Cuts at: 1502 1646 1694 1868 2088 2394
Size: 144 48 174 220 306
Fragments arranged by size:
619 505 306 220 179 174 144 97
48 48 23 19 12
NarI GG'CG_CC
Cuts at: 0 341 370 741 2394
Size: 341 29 371 1653
Fragments arranged by size:
1653 371 341 29
NspI r_CATG'y
Cuts at: 0 1787 2394
Size: 1787 607
PflMI CCAn_nnn'nTGG
Cuts at: 0 98 1878 2394
Size: 98 1780 516
Fragments arranged by size:
1780 516 98
PpuMI rG'GwC_Cy
Cuts at: 0 128 1240 2394
Size: 128 1112 1154
Fragments arranged by size:
1154 1112 128
PsiI TTA'TAA
Cuts at: 0 1157 2394
Size: 1157 1237
PspOMI G'GGCC_C
Cuts at: 0 1373 1600 2394
Size: 1373 227 794
Fragments arranged by size:
1373 794 227
PspXI vC'TCGA_Gb
Cuts at: 0 263 2394
Size: 263 2131
PstI C_TGCA'G
Cuts at: 0 320 548 800 1017 1101 1465 1645 1651
Size: 320 228 252 217 84 364 180 6
Cuts at: 1651 1840 1999 2394
Size: 189 159 395
Fragments arranged by size:
395 364 320 252 228 217 189 180
159 84 6
PvuII CAG'CTG
Cuts at: 0 179 366 883 1502 1646 1694 2088 2394
Size: 179 187 517 619 144 48 394 306
Fragments arranged by size:
619 517 394 306 187 179 144 48
SacI G_AGCT'C
Cuts at: 0 1663 2394
Size: 1663 731
SacII CC_GC'GG
Cuts at: 0 300 348 2394
Size: 300 48 2046
Fragments arranged by size:
2046 300 48
SfcI C'TryA_G
Cuts at: 0 316 544 796 954 1013 1097 1461 1641
Size: 316 228 252 158 59 84 364 180
Cuts at: 1641 1647 1836 1995 2394
Size: 6 189 159 399
Fragments arranged by size:
399 364 316 252 228 189 180 159
158 84 59 6
SfoI GGC'GCC
Cuts at: 0 342 371 742 2394
Size: 342 29 371 1652
Fragments arranged by size:
1652 371 342 29
SmlI C'TyrA_G
Cuts at: 0 263 2394
Size: 263 2131
SphI G_CATG'C
Cuts at: 0 1787 2394
Size: 1787 607
StyI C'CwwG_G
Cuts at: 0 1377 1518 2033 2394
Size: 1377 141 515 361
Fragments arranged by size:
1377 515 361 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 376 571 1351 1456 1565 2394
Size: 376 195 780 105 109 829
Fragments arranged by size:
829 780 376 195 109 105
TatI w'GTAC_w
Cuts at: 0 461 1999 2394
Size: 461 1538 395
Fragments arranged by size:
1538 461 395
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 1561 1593 2343 2375 2394
Size: 437 32 1092 32 750 32 19
Fragments arranged by size:
1092 750 437 32 32 32 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 820 1465 2025 2394
Size: 820 645 560 369
Fragments arranged by size:
820 645 560 369
XhoI C'TCGA_G
Cuts at: 0 263 2394
Size: 263 2131
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI
BciVI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I KasI MluI MscI
MslI MspA1I NarI NspI PflMI PpuMI PsiI PspOMI
PspXI PstI PvuII SacI SacII SfcI SfoI SmlI
SphI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI BaeI BamHI BclI
BfrBI BglI BglII BmtI BpuEI BsaAI BsaBI BsaWI
BsaXI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoNI EcoRI EcoRV FalI FseI HincII
HindIII HpaI KpnI MfeI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsrI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XmaI XmnI ZraI