Restriction map for
pF1KB3027
(Linear) (Six-base) MAPSORT of: KIBB3027.seq Check: 1875 from: 1 to: 3642
KIBB3027.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1162 1697 2345 3642
Size: 1162 535 648 1297
Fragments arranged by size:
1297 1162 648 535
AccI GT'mk_AC
Cuts at: 0 92 2960 3642
Size: 92 2868 682
Fragments arranged by size:
2868 682 92
Acc65I G'GTAC_C
Cuts at: 0 1983 3642
Size: 1983 1659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1657 1941 2306 2904 3020 3642
Size: 1657 284 365 598 116 622
Fragments arranged by size:
1657 622 598 365 284 116
AfeI AGC'GCT
Cuts at: 0 3030 3642
Size: 3030 612
AflIII A'CryG_T
Cuts at: 0 881 2630 3642
Size: 881 1749 1012
Fragments arranged by size:
1749 1012 881
AgeI A'CCGG_T
Cuts at: 0 596 3642
Size: 596 3046
AhdI GACnn_n'nnGTC
Cuts at: 0 3003 3642
Size: 3003 639
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2192 3619 3642
Size: 2192 1427 23
Fragments arranged by size:
2192 1427 23
AlwNI CAG_nnn'CTG
Cuts at: 0 945 1525 1617 1628 2108 3001 3136 3468
Size: 945 580 92 11 480 893 135 332
Cuts at: 3468 3642
Size: 174
Fragments arranged by size:
945 893 580 480 332 174 135 92
11
ApaI G_GGCC'C
Cuts at: 0 1047 1749 2789 3642
Size: 1047 702 1040 853
Fragments arranged by size:
1047 1040 853 702
ApaLI G'TGCA_C
Cuts at: 0 898 3642
Size: 898 2744
ApoI r'AATT_y
Cuts at: 0 2064 3642
Size: 2064 1578
AvaI C'yCGr_G
Cuts at: 0 376 2517 3092 3326 3413 3642
Size: 376 2141 575 234 87 229
Fragments arranged by size:
2141 575 376 234 229 87
BamHI G'GATC_C
Cuts at: 0 1067 3642
Size: 1067 2575
BanI G'GyrC_C
Cuts at: 0 194 1012 1288 1297 1983 2198 3642
Size: 194 818 276 9 686 215 1444
Fragments arranged by size:
1444 818 686 276 215 194 9
BanII G_rGCy'C
Cuts at: 0 1047 1180 1438 1749 1803 2789 2842 2860
Size: 1047 133 258 311 54 986 53 18
Cuts at: 2860 3585 3642
Size: 725 57
Fragments arranged by size:
1047 986 725 311 258 133 57 54
53 18
BbeI G_GCGC'C
Cuts at: 0 198 1292 2202 3642
Size: 198 1094 910 1440
Fragments arranged by size:
1440 1094 910 198
BbsI GAAGACnn'nnnn_
Cuts at: 0 28 703 1089 2704 2894 3642
Size: 28 675 386 1615 190 748
Fragments arranged by size:
1615 748 675 386 190 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 595 629 781 815 2371 2405 3642
Size: 595 34 152 34 1556 34 1237
Fragments arranged by size:
1556 1237 595 152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3117 3642
Size: 3117 525
BclI T'GATC_A
Cuts at: 0 2432 3305 3642
Size: 2432 873 337
Fragments arranged by size:
2432 873 337
BfrBI ATG'CAT
Cuts at: 0 2241 3642
Size: 2241 1401
BglI GCCn_nnn'nGGC
Cuts at: 0 1246 2718 2727 2771 3642
Size: 1246 1472 9 44 871
Fragments arranged by size:
1472 1246 871 44 9
BglII A'GATC_T
Cuts at: 0 728 3642
Size: 728 2914
Bme1580I G_kGCm'C
Cuts at: 0 70 902 993 1000 1047 1541 1749 2789
Size: 70 832 91 7 47 494 208 1040
Cuts at: 2789 3100 3163 3368 3642
Size: 311 63 205 274
Fragments arranged by size:
1040 832 494 311 274 208 205 91
70 63 47 7
BmgBI CAC'GTC
Cuts at: 0 2491 3642
Size: 2491 1151
BmrI ACTGGGnnnn_n'
Cuts at: 0 1752 2843 3642
Size: 1752 1091 799
Fragments arranged by size:
1752 1091 799
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 533 3144 3168 3439 3642
Size: 533 2611 24 271 203
Fragments arranged by size:
2611 533 271 203 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1331 1452 2282 2341 2389 3394 3550
Size: 775 556 121 830 59 48 1005 156
Cuts at: 3550 3642
Size: 92
Fragments arranged by size:
1005 830 775 556 156 121 92 59
48
Bpu10I CC'TnA_GC
Cuts at: 0 1179 2501 3642
Size: 1179 1322 1141
Fragments arranged by size:
1322 1179 1141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1747 2098 3642
Size: 1747 351 1544
Fragments arranged by size:
1747 1544 351
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 246 3619 3642
Size: 70 176 3373 23
Fragments arranged by size:
3373 176 70 23
BsaHI Gr'CG_yC
Cuts at: 0 195 1289 2199 3642
Size: 195 1094 910 1443
Fragments arranged by size:
1443 1094 910 195
BsaWI w'CCGG_w
Cuts at: 0 596 3041 3642
Size: 596 2445 601
Fragments arranged by size:
2445 601 596
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 748 778 3642
Size: 748 30 2864
Fragments arranged by size:
2864 748 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 634 670 1408 1423 1426 2095 2602
Size: 394 240 36 738 15 3 669 507
Cuts at: 2602 3642
Size: 1040
Fragments arranged by size:
1040 738 669 507 394 240 36 15
3
BseYI C'CCAG_C
Cuts at: 0 207 226 284 1040 1241 2761 2792 3297
Size: 207 19 58 756 201 1520 31 505
Cuts at: 3297 3642
Size: 345
Fragments arranged by size:
1520 756 505 345 207 201 58 31
19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 3642
Size: 1603 2039
BsiHKAI G_wGCw'C
Cuts at: 0 514 902 1127 1438 1803 2186 2860 3642
Size: 514 388 225 311 365 383 674 782
Fragments arranged by size:
782 674 514 388 383 365 311 225
BsmI GAATG_Cn'
Cuts at: 0 2394 3642
Size: 2394 1248
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1843 2224 2476 3642
Size: 1843 381 252 1166
Fragments arranged by size:
1843 1166 381 252
Bsp1286I G_dGCh'C
Cuts at: 0 70 514 902 993 1000 1047 1127 1180
Size: 70 444 388 91 7 47 80 53
Cuts at: 1180 1438 1541 1749 1803 2186 2789 2842 2860
Size: 258 103 208 54 383 603 53 18
Cuts at: 2860 3100 3163 3368 3585 3642
Size: 240 63 205 217 57
Fragments arranged by size:
603 444 388 383 258 240 217 208
205 103 91 80 70 63 57 54
53 53 47 18 7
BspHI T'CATG_A
Cuts at: 0 680 2804 3642
Size: 680 2124 838
Fragments arranged by size:
2124 838 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 88 1162 1697 2345 3642
Size: 88 1074 535 648 1297
Fragments arranged by size:
1297 1074 648 535 88
BsrBI CCG'CTC
Cuts at: 0 2307 2373 2414 2505 3642
Size: 2307 66 41 91 1137
Fragments arranged by size:
2307 1137 91 66 41
BsrDI GCAATG_nn'
Cuts at: 0 843 867 1663 3642
Size: 843 24 796 1979
Fragments arranged by size:
1979 843 796 24
BsrFI r'CCGG_y
Cuts at: 0 596 1965 2726 3642
Size: 596 1369 761 916
Fragments arranged by size:
1369 916 761 596
BssSI C'ACGA_G
Cuts at: 0 974 1810 3262 3642
Size: 974 836 1452 380
Fragments arranged by size:
1452 974 836 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1196 1617 3468 3642
Size: 1196 421 1851 174
Fragments arranged by size:
1851 1196 421 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1142 1249 3642
Size: 1142 107 2393
Fragments arranged by size:
2393 1142 107
BstYI r'GATC_y
Cuts at: 0 728 1067 1496 3642
Size: 728 339 429 2146
Fragments arranged by size:
2146 728 429 339
BstZ17I GTA'TAC
Cuts at: 0 2961 3642
Size: 2961 681
Bsu36I CC'TnA_GG
Cuts at: 0 932 1073 3129 3642
Size: 932 141 2056 513
Fragments arranged by size:
2056 932 513 141
BtgI C'CryG_G
Cuts at: 0 1874 2015 2766 3155 3168 3345 3642
Size: 1874 141 751 389 13 177 297
Fragments arranged by size:
1874 751 389 297 177 141 13
BtsI GCAGTG_nn'
Cuts at: 0 2159 2452 3123 3469 3627 3642
Size: 2159 293 671 346 158 15
Fragments arranged by size:
2159 671 346 293 158 15
DraIII CAC_nnn'GTG
Cuts at: 0 2422 3642
Size: 2422 1220
EaeI y'GGCC_r
Cuts at: 0 312 580 893 3165 3642
Size: 312 268 313 2272 477
Fragments arranged by size:
2272 477 313 312 268
EarI CTCTTCn'nnn_
Cuts at: 0 877 1591 2297 2941 3190 3442 3642
Size: 877 714 706 644 249 252 200
Fragments arranged by size:
877 714 706 644 252 249 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2176 2475 3642
Size: 2176 299 1167
Fragments arranged by size:
2176 1167 299
EcoICRI GAG'CTC
Cuts at: 0 1436 1801 2858 3642
Size: 1436 365 1057 784
Fragments arranged by size:
1436 1057 784 365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1331 1452 1657 1941 2282 2306 2341
Size: 775 556 121 205 284 341 24 35
Cuts at: 2341 2389 2904 3020 3394 3550 3642
Size: 48 515 116 374 156 92
Fragments arranged by size:
775 556 515 374 341 284 205 156
121 116 92 48 35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3514 3576 3642
Size: 3514 62 66
Fragments arranged by size:
3514 66 62
EcoO109I rG'GnC_Cy
Cuts at: 0 384 1184 1659 1746 2623 2785 2786 3507
Size: 384 800 475 87 877 162 1 721
Cuts at: 3507 3642
Size: 135
Fragments arranged by size:
877 800 721 475 384 162 135 87
1
EcoRV GAT'ATC
Cuts at: 0 678 3642
Size: 678 2964
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2058 2970 3642
Size: 2058 912 672
Fragments arranged by size:
2058 912 672
FseI GG_CCGG'CC
Cuts at: 0 2730 3642
Size: 2730 912
HaeII r_GCGC'y
Cuts at: 0 198 1041 1292 2202 3032 3642
Size: 198 843 251 910 830 610
Fragments arranged by size:
910 843 830 610 251 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 748 780 2989 3021 3223 3255
Size: 340 32 376 32 2209 32 202 32
Cuts at: 3255 3297 3329 3642
Size: 42 32 313
Fragments arranged by size:
2209 376 340 313 202 42 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 2464 3642
Size: 2464 1178
KasI G'GCGC_C
Cuts at: 0 194 1288 2198 3642
Size: 194 1094 910 1444
Fragments arranged by size:
1444 1094 910 194
KpnI G_GTAC'C
Cuts at: 0 1987 3642
Size: 1987 1655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 673 3569 3642
Size: 503 170 2896 73
Fragments arranged by size:
2896 503 170 73
MslI CAynn'nnrTG
Cuts at: 0 63 497 1264 1879 3361 3642
Size: 63 434 767 615 1482 281
Fragments arranged by size:
1482 767 615 434 281 63
MspA1I CmG'CkG
Cuts at: 0 427 1776 1887 2704 2924 3170 3246 3642
Size: 427 1349 111 817 220 246 76 396
Fragments arranged by size:
1349 817 427 396 246 220 111 76
NaeI GCC'GGC
Cuts at: 0 2728 3642
Size: 2728 914
NarI GG'CG_CC
Cuts at: 0 195 1289 2199 3642
Size: 195 1094 910 1443
Fragments arranged by size:
1443 1094 910 195
NcoI C'CATG_G
Cuts at: 0 1874 2015 3345 3642
Size: 1874 141 1330 297
Fragments arranged by size:
1874 1330 297 141
NgoMIV G'CCGG_C
Cuts at: 0 2726 3642
Size: 2726 916
NsiI A_TGCA'T
Cuts at: 0 2243 3642
Size: 2243 1399
NspI r_CATG'y
Cuts at: 0 885 1238 2241 2634 3642
Size: 885 353 1003 393 1008
Fragments arranged by size:
1008 1003 885 393 353
PciI A'CATG_T
Cuts at: 0 881 2630 3642
Size: 881 1749 1012
Fragments arranged by size:
1749 1012 881
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 2256 2914 3186 3642
Size: 291 1965 658 272 456
Fragments arranged by size:
1965 658 456 291 272
PfoI T'CCnGG_A
Cuts at: 0 1468 3642
Size: 1468 2174
PpuMI rG'GwC_Cy
Cuts at: 0 1659 2623 3507 3642
Size: 1659 964 884 135
Fragments arranged by size:
1659 964 884 135
PspOMI G'GGCC_C
Cuts at: 0 1043 1745 2785 3642
Size: 1043 702 1040 857
Fragments arranged by size:
1043 1040 857 702
PspXI vC'TCGA_Gb
Cuts at: 0 2517 3642
Size: 2517 1125
PstI C_TGCA'G
Cuts at: 0 618 965 1682 2977 3590 3642
Size: 618 347 717 1295 613 52
Fragments arranged by size:
1295 717 618 613 347 52
PvuII CAG'CTG
Cuts at: 0 427 1887 2704 3642
Size: 427 1460 817 938
Fragments arranged by size:
1460 938 817 427
SacI G_AGCT'C
Cuts at: 0 1438 1803 2860 3642
Size: 1438 365 1057 782
Fragments arranged by size:
1438 1057 782 365
SacII CC_GC'GG
Cuts at: 0 3171 3642
Size: 3171 471
SanDI GG'GwC_CC
Cuts at: 0 1659 2623 3642
Size: 1659 964 1019
Fragments arranged by size:
1659 1019 964
SapI GCTCTTCn'nnn_
Cuts at: 0 1591 2297 3642
Size: 1591 706 1345
Fragments arranged by size:
1591 1345 706
SfcI C'TryA_G
Cuts at: 0 614 961 1678 2973 3586 3642
Size: 614 347 717 1295 613 56
Fragments arranged by size:
1295 717 614 613 347 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2727 3642
Size: 2727 915
SfoI GGC'GCC
Cuts at: 0 196 1290 2200 3642
Size: 196 1094 910 1442
Fragments arranged by size:
1442 1094 910 196
SmlI C'TyrA_G
Cuts at: 0 1762 2113 2517 3642
Size: 1762 351 404 1125
Fragments arranged by size:
1762 1125 404 351
SphI G_CATG'C
Cuts at: 0 2241 3642
Size: 2241 1401
StuI AGG'CCT
Cuts at: 0 2618 3148 3642
Size: 2618 530 494
Fragments arranged by size:
2618 530 494
StyI C'CwwG_G
Cuts at: 0 1457 1874 1919 2015 2126 3345 3642
Size: 1457 417 45 96 111 1219 297
Fragments arranged by size:
1457 1219 417 297 111 96 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 764 3484 3642
Size: 240 524 2720 158
Fragments arranged by size:
2720 524 240 158
TatI w'GTAC_w
Cuts at: 0 3561 3642
Size: 3561 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1481 1513 3250 3282 3642
Size: 1481 32 1737 32 360
Fragments arranged by size:
1737 1481 360 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1139 2257 3480 3642
Size: 1139 1118 1223 162
Fragments arranged by size:
1223 1139 1118 162
XhoI C'TCGA_G
Cuts at: 0 2517 3642
Size: 2517 1125
XmnI GAAnn'nnTTC
Cuts at: 0 25 2950 3642
Size: 25 2925 692
Fragments arranged by size:
2925 692 25
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FseI HaeII Hin4I HindIII KasI KpnI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SanDI SapI SfcI SfiI SfoI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AclI AflII AleI AloI AscI AseI AsiSI
AvrII BaeI BbvCI BlpI BmtI BsaAI BsaBI BsiEI
BsiWI BspEI BsrGI BssHII BstBI BstEII BtgZI ClaI
CspCI DraI DrdI EagI EcoRI FspI FspAI HincII
HpaI MfeI MluI MscI NdeI NheI NotI NruI
PacI PasI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XmaI ZraI