Restriction map for
pF1KB3028
(Linear) (Six-base) MAPSORT of: KIBB3028.seq Check: 6937 from: 1 to: 3552
KIBB3028.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1769 2344 3533 3552
Size: 1769 575 1189 19
Fragments arranged by size:
1769 1189 575 19
AccI GT'mk_AC
Cuts at: 0 921 3552
Size: 921 2631
Acc65I G'GTAC_C
Cuts at: 0 676 3552
Size: 676 2876
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 1182 2659 3083 3552
Size: 867 315 1477 424 469
Fragments arranged by size:
1477 867 469 424 315
AfeI AGC'GCT
Cuts at: 0 3370 3552
Size: 3370 182
AflIII A'CryG_T
Cuts at: 0 1229 3552
Size: 1229 2323
AleI CACnn'nnGTG
Cuts at: 0 2092 3552
Size: 2092 1460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2161 2585 3493 3552
Size: 2161 424 908 59
Fragments arranged by size:
2161 908 424 59
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 3552
Size: 823 32 2697
Fragments arranged by size:
2697 823 32
AlwNI CAG_nnn'CTG
Cuts at: 0 156 423 811 2793 2895 3552
Size: 156 267 388 1982 102 657
Fragments arranged by size:
1982 657 388 267 156 102
ApaI G_GGCC'C
Cuts at: 0 562 1798 2783 3552
Size: 562 1236 985 769
Fragments arranged by size:
1236 985 769 562
AseI AT'TA_AT
Cuts at: 0 1472 3552
Size: 1472 2080
AvaI C'yCGr_G
Cuts at: 0 1881 2060 2576 2596 3552
Size: 1881 179 516 20 956
Fragments arranged by size:
1881 956 516 179 20
BamHI G'GATC_C
Cuts at: 0 303 458 3057 3552
Size: 303 155 2599 495
Fragments arranged by size:
2599 495 303 155
BanI G'GyrC_C
Cuts at: 0 676 1767 3282 3545 3552
Size: 676 1091 1515 263 7
Fragments arranged by size:
1515 1091 676 263 7
BanII G_rGCy'C
Cuts at: 0 477 562 1798 1901 2067 2603 2783 2855
Size: 477 85 1236 103 166 536 180 72
Cuts at: 2855 3298 3343 3552
Size: 443 45 209
Fragments arranged by size:
1236 536 477 443 209 180 166 103
85 72 45
BglI GCCn_nnn'nGGC
Cuts at: 0 1802 2292 3172 3302 3400 3552
Size: 1802 490 880 130 98 152
Fragments arranged by size:
1802 880 490 152 130 98
BlpI GC'TnA_GC
Cuts at: 0 256 1177 1336 1708 3148 3292 3552
Size: 256 921 159 372 1440 144 260
Fragments arranged by size:
1440 921 372 260 256 159 144
Bme1580I G_kGCm'C
Cuts at: 0 562 1772 1798 1807 2783 3552
Size: 562 1210 26 9 976 769
Fragments arranged by size:
1210 976 769 562 26 9
BmgBI CAC'GTC
Cuts at: 0 1059 3552
Size: 1059 2493
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 521 1501 3552
Size: 166 355 980 2051
Fragments arranged by size:
2051 980 355 166
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 461 1918 3090 3357 3411 3552
Size: 459 2 1457 1172 267 54 141
Fragments arranged by size:
1457 1172 459 267 141 54 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1294 2038 2693 3038 3357 3370 3552
Size: 848 446 744 655 345 319 13 182
Fragments arranged by size:
848 744 655 446 345 319 182 13
Bpu10I CC'TnA_GC
Cuts at: 0 2341 3552
Size: 2341 1211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2154 3552
Size: 2154 1398
BsaI GGTCTCn'nnnn_
Cuts at: 0 562 2669 3552
Size: 562 2107 883
Fragments arranged by size:
2107 883 562
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 507 537 823 853 2251 2281 3552
Size: 507 30 286 30 1398 30 1271
Fragments arranged by size:
1398 1271 507 286 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 2706 2858 2863 3259 3552
Size: 181 2525 152 5 396 293
Fragments arranged by size:
2525 396 293 181 152 5
BseYI C'CCAG_C
Cuts at: 0 24 184 996 1073 1188 1797 2420 2603
Size: 24 160 812 77 115 609 623 183
Cuts at: 2603 2888 3121 3552
Size: 285 233 431
Fragments arranged by size:
812 623 609 431 285 233 183 160
115 77 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 755 3479 3552
Size: 490 265 2724 73
Fragments arranged by size:
2724 490 265 73
BsiHKAI G_wGCw'C
Cuts at: 0 477 536 1709 2342 2502 2855 3343 3552
Size: 477 59 1173 633 160 353 488 209
Fragments arranged by size:
1173 633 488 477 353 209 160 59
BsmI GAATG_Cn'
Cuts at: 0 1340 1749 2574 3552
Size: 1340 409 825 978
Fragments arranged by size:
1340 978 825 409
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2724 3552
Size: 2724 828
Bsp1286I G_dGCh'C
Cuts at: 0 477 536 562 1709 1772 1798 1807 1901
Size: 477 59 26 1147 63 26 9 94
Cuts at: 1901 2067 2342 2502 2603 2783 2855 3298 3343
Size: 166 275 160 101 180 72 443 45
Cuts at: 3343 3552
Size: 209
Fragments arranged by size:
1147 477 443 275 209 180 166 160
101 94 72 63 59 45 26 26
9
BspHI T'CATG_A
Cuts at: 0 1452 3552
Size: 1452 2100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1379 1769 2344 3533 3552
Size: 1379 390 575 1189 19
Fragments arranged by size:
1379 1189 575 390 19
BsrBI CCG'CTC
Cuts at: 0 2835 3552
Size: 2835 717
BsrDI GCAATG_nn'
Cuts at: 0 1002 1923 3552
Size: 1002 921 1629
Fragments arranged by size:
1629 1002 921
BsrFI r'CCGG_y
Cuts at: 0 1585 2502 3143 3552
Size: 1585 917 641 409
Fragments arranged by size:
1585 917 641 409
BsrGI T'GTAC_A
Cuts at: 0 2465 3552
Size: 2465 1087
BssSI C'ACGA_G
Cuts at: 0 2329 3306 3552
Size: 2329 977 246
Fragments arranged by size:
2329 977 246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 12 21 1629 1873 3545 3552
Size: 12 9 1608 244 1672 7
Fragments arranged by size:
1672 1608 244 12 9 7
BstEII G'GTnAC_C
Cuts at: 0 78 3552
Size: 78 3474
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 1791 3552
Size: 234 1557 1761
Fragments arranged by size:
1761 1557 234
BstYI r'GATC_y
Cuts at: 0 303 365 458 2789 3057 3552
Size: 303 62 93 2331 268 495
Fragments arranged by size:
2331 495 303 268 93 62
Bsu36I CC'TnA_GG
Cuts at: 0 215 2104 3465 3552
Size: 215 1889 1361 87
Fragments arranged by size:
1889 1361 215 87
BtgI C'CryG_G
Cuts at: 0 397 2843 3239 3383 3552
Size: 397 2446 396 144 169
Fragments arranged by size:
2446 397 396 169 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1727 2419 3552
Size: 1727 692 1133
Fragments arranged by size:
1727 1133 692
BtsI GCAGTG_nn'
Cuts at: 0 1167 1270 1550 2691 3343 3552
Size: 1167 103 280 1141 652 209
Fragments arranged by size:
1167 1141 652 280 209 103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1844 1879 3552
Size: 1844 35 1673
Fragments arranged by size:
1844 1673 35
DraIII CAC_nnn'GTG
Cuts at: 0 2331 3552
Size: 2331 1221
EaeI y'GGCC_r
Cuts at: 0 1583 1823 1840 2957 3314 3449 3552
Size: 1583 240 17 1117 357 135 103
Fragments arranged by size:
1583 1117 357 240 135 103 17
EarI CTCTTCn'nnn_
Cuts at: 0 1103 1433 1571 1942 3059 3092 3552
Size: 1103 330 138 371 1117 33 460
Fragments arranged by size:
1117 1103 460 371 330 138 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 189 2521 3552
Size: 189 2332 1031
Fragments arranged by size:
2332 1031 189
EcoICRI GAG'CTC
Cuts at: 0 475 2853 3341 3552
Size: 475 2378 488 211
Fragments arranged by size:
2378 488 475 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 867 1182 1294 2038 2659 2693 3038
Size: 848 19 315 112 744 621 34 345
Cuts at: 3038 3083 3357 3370 3552
Size: 45 274 13 182
Fragments arranged by size:
848 744 621 345 315 274 182 112
45 34 19 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 815 2612 3552
Size: 815 1797 940
Fragments arranged by size:
1797 940 815
EcoO109I rG'GnC_Cy
Cuts at: 0 493 559 820 975 982 2129 2243 2294
Size: 493 66 261 155 7 1147 114 51
Cuts at: 2294 2424 2439 2779 2780 3049 3069 3254 3431
Size: 130 15 340 1 269 20 185 177
Cuts at: 3431 3486 3552
Size: 55 66
Fragments arranged by size:
1147 493 340 269 261 185 177 155
130 114 66 66 55 51 20 15
7 1
HaeII r_GCGC'y
Cuts at: 0 223 443 3155 3372 3406 3552
Size: 223 220 2712 217 34 146
Fragments arranged by size:
2712 223 220 217 146 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1864 1896 2251 2283 2758 2790 3552
Size: 1864 32 355 32 475 32 762
Fragments arranged by size:
1864 762 475 355 32 32 32
HincII GTy'rAC
Cuts at: 0 641 696 922 2538 3552
Size: 641 55 226 1616 1014
Fragments arranged by size:
1616 1014 641 226 55
HpaI GTT'AAC
Cuts at: 0 641 3552
Size: 641 2911
KpnI G_GTAC'C
Cuts at: 0 680 3552
Size: 680 2872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1559 3552
Size: 1559 1993
MscI TGG'CCA
Cuts at: 0 1825 2959 3316 3451 3552
Size: 1825 1134 357 135 101
Fragments arranged by size:
1825 1134 357 135 101
MslI CAynn'nnrTG
Cuts at: 0 232 592 1228 2092 2143 2387 2747 3382
Size: 232 360 636 864 51 244 360 635
Cuts at: 3382 3475 3552
Size: 93 77
Fragments arranged by size:
864 636 635 360 360 244 232 93
77 51
MspA1I CmG'CkG
Cuts at: 0 210 288 1619 1801 1965 2892 3552
Size: 210 78 1331 182 164 927 660
Fragments arranged by size:
1331 927 660 210 182 164 78
NaeI GCC'GGC
Cuts at: 0 1587 3552
Size: 1587 1965
NgoMIV G'CCGG_C
Cuts at: 0 1585 3552
Size: 1585 1967
NspI r_CATG'y
Cuts at: 0 580 861 1233 2142 3381 3552
Size: 580 281 372 909 1239 171
Fragments arranged by size:
1239 909 580 372 281 171
PciI A'CATG_T
Cuts at: 0 1229 3552
Size: 1229 2323
PflMI CCAn_nnn'nTGG
Cuts at: 0 156 375 1188 1764 3552
Size: 156 219 813 576 1788
Fragments arranged by size:
1788 813 576 219 156
PfoI T'CCnGG_A
Cuts at: 0 1496 3552
Size: 1496 2056
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 3552
Size: 823 32 2697
Fragments arranged by size:
2697 823 32
PpuMI rG'GwC_Cy
Cuts at: 0 975 982 2424 3069 3254 3431 3486 3552
Size: 975 7 1442 645 185 177 55 66
Fragments arranged by size:
1442 975 645 185 177 66 55 7
PshAI GACnn'nnGTC
Cuts at: 0 980 3552
Size: 980 2572
PspOMI G'GGCC_C
Cuts at: 0 558 1794 2779 3552
Size: 558 1236 985 773
Fragments arranged by size:
1236 985 773 558
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 934 966 3552
Size: 934 32 2586
Fragments arranged by size:
2586 934 32
PstI C_TGCA'G
Cuts at: 0 2438 3237 3552
Size: 2438 799 315
Fragments arranged by size:
2438 799 315
PvuII CAG'CTG
Cuts at: 0 210 288 1965 2892 3552
Size: 210 78 1677 927 660
Fragments arranged by size:
1677 927 660 210 78
SacI G_AGCT'C
Cuts at: 0 477 2855 3343 3552
Size: 477 2378 488 209
Fragments arranged by size:
2378 488 477 209
SalI G'TCGA_C
Cuts at: 0 920 3552
Size: 920 2632
SanDI GG'GwC_CC
Cuts at: 0 982 2424 3486 3552
Size: 982 1442 1062 66
Fragments arranged by size:
1442 1062 982 66
SapI GCTCTTCn'nnn_
Cuts at: 0 1103 3092 3552
Size: 1103 1989 460
Fragments arranged by size:
1989 1103 460
SbfI CC_TGCA'GG
Cuts at: 0 2438 3552
Size: 2438 1114
ScaI AGT'ACT
Cuts at: 0 2953 3552
Size: 2953 599
SexAI A'CCwGG_T
Cuts at: 0 774 3552
Size: 774 2778
SfcI C'TryA_G
Cuts at: 0 1905 2434 3233 3552
Size: 1905 529 799 319
Fragments arranged by size:
1905 799 529 319
SgrAI Cr'CCGG_yG
Cuts at: 0 2502 3552
Size: 2502 1050
SmaI CCC'GGG
Cuts at: 0 2578 3552
Size: 2578 974
SmlI C'TyrA_G
Cuts at: 0 2133 3552
Size: 2133 1419
SphI G_CATG'C
Cuts at: 0 3381 3552
Size: 3381 171
StuI AGG'CCT
Cuts at: 0 2634 3552
Size: 2634 918
StyI C'CwwG_G
Cuts at: 0 542 816 1103 1302 3552
Size: 542 274 287 199 2250
Fragments arranged by size:
2250 542 287 274 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3408 3552
Size: 3408 144
TatI w'GTAC_w
Cuts at: 0 2169 2465 2951 3552
Size: 2169 296 486 601
Fragments arranged by size:
2169 601 486 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 507 539 740 772 3552
Size: 507 32 201 32 2780
Fragments arranged by size:
2780 507 201 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2533 3552
Size: 2533 1019
XbaI T'CTAG_A
Cuts at: 0 927 3552
Size: 927 2625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 823 831 1248 2664 3129 3381 3552
Size: 342 481 8 417 1416 465 252 171
Fragments arranged by size:
1416 481 465 417 342 252 171 8
XmaI C'CCGG_G
Cuts at: 0 2576 3552
Size: 2576 976
XmnI GAAnn'nnTTC
Cuts at: 0 1097 3552
Size: 1097 2455
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI AseI AvaI BamHI BanI BanII
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII HpaI KpnI MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI
PfoI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgrAI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI ApaLI ApoI AscI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmtI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BspEI BssHII BstBI BstZ17I ClaI DraI
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HindIII KasI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PmlI PsiI
PspXI PvuI RsrII SacII SfiI SfoI SgfI SnaBI
SpeI SrfI SspI SwaI XhoI ZraI