Restriction map for
pF1KB3029
(Linear) (Six-base) MAPSORT of: KIBB3029.seq Check: 5536 from: 1 to: 2031
KIBB3029.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 584 2031
Size: 584 1447
AccI GT'mk_AC
Cuts at: 0 1789 2031
Size: 1789 242
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1307 2031
Size: 708 599 724
Fragments arranged by size:
724 708 599
AfeI AGC'GCT
Cuts at: 0 695 2031
Size: 695 1336
AhdI GACnn_n'nnGTC
Cuts at: 0 1065 2031
Size: 1065 966
AlwNI CAG_nnn'CTG
Cuts at: 0 255 2031
Size: 255 1776
ApaI G_GGCC'C
Cuts at: 0 1898 2031
Size: 1898 133
ApaLI G'TGCA_C
Cuts at: 0 72 2031
Size: 72 1959
ApoI r'AATT_y
Cuts at: 0 583 796 2031
Size: 583 213 1235
Fragments arranged by size:
1235 583 213
AvaI C'yCGr_G
Cuts at: 0 675 1195 1681 2031
Size: 675 520 486 350
Fragments arranged by size:
675 520 486 350
BanI G'GyrC_C
Cuts at: 0 269 359 668 1162 2031
Size: 269 90 309 494 869
Fragments arranged by size:
869 494 309 269 90
BanII G_rGCy'C
Cuts at: 0 521 1154 1493 1898 1961 2031
Size: 521 633 339 405 63 70
Fragments arranged by size:
633 521 405 339 70 63
BbeI G_GCGC'C
Cuts at: 0 273 672 2031
Size: 273 399 1359
Fragments arranged by size:
1359 399 273
BbsI GAAGACnn'nnnn_
Cuts at: 0 809 2031
Size: 809 1222
BbvCI CC'TCA_GC
Cuts at: 0 454 1636 2031
Size: 454 1182 395
Fragments arranged by size:
1182 454 395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 21 55 2031
Size: 21 34 1976
Fragments arranged by size:
1976 34 21
BfrBI ATG'CAT
Cuts at: 0 717 1289 2031
Size: 717 572 742
Fragments arranged by size:
742 717 572
BglI GCCn_nnn'nGGC
Cuts at: 0 677 1036 1567 1866 2031
Size: 677 359 531 299 165
Fragments arranged by size:
677 531 359 299 165
BglII A'GATC_T
Cuts at: 0 1598 2031
Size: 1598 433
BlpI GC'TnA_GC
Cuts at: 0 1856 2031
Size: 1856 175
Bme1580I G_kGCm'C
Cuts at: 0 76 433 1167 1898 1974 2031
Size: 76 357 734 731 76 57
Fragments arranged by size:
734 731 357 76 76 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 712 2031
Size: 712 1319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 451 874 961 1384 1587 1606 2031
Size: 115 336 423 87 423 203 19 425
Fragments arranged by size:
425 423 423 336 203 115 87 19
Bpu10I CC'TnA_GC
Cuts at: 0 454 1636 2031
Size: 454 1182 395
Fragments arranged by size:
1182 454 395
BsaAI yAC'GTr
Cuts at: 0 222 390 483 2031
Size: 222 168 93 1548
Fragments arranged by size:
1548 222 168 93
BsaHI Gr'CG_yC
Cuts at: 0 270 669 1723 2031
Size: 270 399 1054 308
Fragments arranged by size:
1054 399 308 270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 847 877 2031
Size: 847 30 1154
Fragments arranged by size:
1154 847 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 871 874 958 1099 1117 1284 1933
Size: 141 730 3 84 141 18 167 649
Cuts at: 1933 1945 1948 1960 2031
Size: 12 3 12 71
Fragments arranged by size:
730 649 167 141 141 84 71 18
12 12 3 3
BseYI C'CCAG_C
Cuts at: 0 886 2031
Size: 886 1145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 2031
Size: 142 1889
BsiEI CG_ry'CG
Cuts at: 0 158 375 2031
Size: 158 217 1656
Fragments arranged by size:
1656 217 158
BsiHKAI G_wGCw'C
Cuts at: 0 76 521 1154 1493 1628 2031
Size: 76 445 633 339 135 403
Fragments arranged by size:
633 445 403 339 135 76
BsiWI C'GTAC_G
Cuts at: 0 421 2031
Size: 421 1610
BsmI GAATG_Cn'
Cuts at: 0 719 1291 2031
Size: 719 572 740
Fragments arranged by size:
740 719 572
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1322 2031
Size: 1322 709
Bsp1286I G_dGCh'C
Cuts at: 0 76 433 521 1154 1167 1493 1628 1898
Size: 76 357 88 633 13 326 135 270
Cuts at: 1898 1961 1974 2031
Size: 63 13 57
Fragments arranged by size:
633 357 326 270 135 88 76 63
57 13 13
BspHI T'CATG_A
Cuts at: 0 839 2031
Size: 839 1192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 584 1451 2031
Size: 470 114 867 580
Fragments arranged by size:
867 580 470 114
BsrBI CCG'CTC
Cuts at: 0 1892 2031
Size: 1892 139
BsrFI r'CCGG_y
Cuts at: 0 534 665 1004 1645 1725 2031
Size: 534 131 339 641 80 306
Fragments arranged by size:
641 534 339 306 131 80
BssHII G'CGCG_C
Cuts at: 0 55 2031
Size: 55 1976
BssSI C'ACGA_G
Cuts at: 0 601 2031
Size: 601 1430
BstAPI GCAn_nnn'nTGC
Cuts at: 0 80 255 2031
Size: 80 175 1776
Fragments arranged by size:
1776 175 80
BstYI r'GATC_y
Cuts at: 0 1598 2031
Size: 1598 433
Bsu36I CC'TnA_GG
Cuts at: 0 622 2031
Size: 622 1409
BtgI C'CryG_G
Cuts at: 0 18 158 369 1115 1814 2031
Size: 18 140 211 746 699 217
Fragments arranged by size:
746 699 217 211 140 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2027 2031
Size: 2027 4
BtsI GCAGTG_nn'
Cuts at: 0 312 1247 2031
Size: 312 935 784
Fragments arranged by size:
935 784 312
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1790 1825 2031
Size: 1790 35 206
Fragments arranged by size:
1790 206 35
DraIII CAC_nnn'GTG
Cuts at: 0 606 2031
Size: 606 1425
EaeI y'GGCC_r
Cuts at: 0 155 347 372 536 1006 1028 1118 1727
Size: 155 192 25 164 470 22 90 609
Cuts at: 1727 1811 2031
Size: 84 220
Fragments arranged by size:
609 470 220 192 164 155 90 84
25 22
EagI C'GGCC_G
Cuts at: 0 155 372 2031
Size: 155 217 1659
Fragments arranged by size:
1659 217 155
EarI CTCTTCn'nnn_
Cuts at: 0 778 2031
Size: 778 1253
EcoICRI GAG'CTC
Cuts at: 0 519 1152 1491 2031
Size: 519 633 339 540
Fragments arranged by size:
633 540 519 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 451 708 874 961 1307 1384 1587
Size: 115 336 257 166 87 346 77 203
Cuts at: 1587 1606 2031
Size: 19 425
Fragments arranged by size:
425 346 336 257 203 166 115 87
77 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 856 2031
Size: 466 390 1175
Fragments arranged by size:
1175 466 390
EcoO109I rG'GnC_Cy
Cuts at: 0 1023 1226 1632 1868 2031
Size: 1023 203 406 236 163
Fragments arranged by size:
1023 406 236 203 163
EcoRI G'AATT_C
Cuts at: 0 583 2031
Size: 583 1448
FspI TGC'GCA
Cuts at: 0 572 2031
Size: 572 1459
HaeII r_GCGC'y
Cuts at: 0 273 672 697 1646 1694 2031
Size: 273 399 25 949 48 337
Fragments arranged by size:
949 399 337 273 48 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 859 891 1051 1083 2031
Size: 859 32 160 32 948
Fragments arranged by size:
948 859 160 32 32
HincII GTy'rAC
Cuts at: 0 824 2031
Size: 824 1207
KasI G'GCGC_C
Cuts at: 0 269 668 2031
Size: 269 399 1363
Fragments arranged by size:
1363 399 269
MfeI C'AATT_G
Cuts at: 0 980 2031
Size: 980 1051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1373 2031
Size: 1373 658
MscI TGG'CCA
Cuts at: 0 1030 1813 2031
Size: 1030 783 218
Fragments arranged by size:
1030 783 218
MslI CAynn'nnrTG
Cuts at: 0 109 751 2031
Size: 109 642 1280
Fragments arranged by size:
1280 642 109
MspA1I CmG'CkG
Cuts at: 0 20 140 297 303 371 886 1117 2031
Size: 20 120 157 6 68 515 231 914
Fragments arranged by size:
914 515 231 157 120 68 20 6
NaeI GCC'GGC
Cuts at: 0 536 667 1006 1647 1727 2031
Size: 536 131 339 641 80 304
Fragments arranged by size:
641 536 339 304 131 80
NarI GG'CG_CC
Cuts at: 0 270 669 2031
Size: 270 399 1362
Fragments arranged by size:
1362 399 270
NgoMIV G'CCGG_C
Cuts at: 0 534 665 1004 1645 1725 2031
Size: 534 131 339 641 80 306
Fragments arranged by size:
641 534 339 306 131 80
NsiI A_TGCA'T
Cuts at: 0 719 1291 2031
Size: 719 572 740
Fragments arranged by size:
740 719 572
NspI r_CATG'y
Cuts at: 0 444 2031
Size: 444 1587
PasI CC'CwG_GG
Cuts at: 0 1221 2031
Size: 1221 810
PflMI CCAn_nnn'nTGG
Cuts at: 0 285 2031
Size: 285 1746
PfoI T'CCnGG_A
Cuts at: 0 446 2031
Size: 446 1585
PmlI CAC'GTG
Cuts at: 0 222 390 2031
Size: 222 168 1641
Fragments arranged by size:
1641 222 168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 2031
Size: 109 32 1890
Fragments arranged by size:
1890 109 32
PpuMI rG'GwC_Cy
Cuts at: 0 1226 2031
Size: 1226 805
PspOMI G'GGCC_C
Cuts at: 0 1894 2031
Size: 1894 137
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1423 1455 2031
Size: 1423 32 576
Fragments arranged by size:
1423 576 32
PstI C_TGCA'G
Cuts at: 0 260 302 1254 1979 2031
Size: 260 42 952 725 52
Fragments arranged by size:
952 725 260 52 42
PvuII CAG'CTG
Cuts at: 0 297 303 886 2031
Size: 297 6 583 1145
Fragments arranged by size:
1145 583 297 6
SacI G_AGCT'C
Cuts at: 0 521 1154 1493 2031
Size: 521 633 339 538
Fragments arranged by size:
633 538 521 339
SacII CC_GC'GG
Cuts at: 0 21 372 1118 2031
Size: 21 351 746 913
Fragments arranged by size:
913 746 351 21
SanDI GG'GwC_CC
Cuts at: 0 1226 2031
Size: 1226 805
SexAI A'CCwGG_T
Cuts at: 0 410 2031
Size: 410 1621
SfcI C'TryA_G
Cuts at: 0 256 298 631 1250 1975 2031
Size: 256 42 333 619 725 56
Fragments arranged by size:
725 619 333 256 56 42
SfoI GGC'GCC
Cuts at: 0 271 670 2031
Size: 271 399 1361
Fragments arranged by size:
1361 399 271
SgrAI Cr'CCGG_yG
Cuts at: 0 1645 2031
Size: 1645 386
StuI AGG'CCT
Cuts at: 0 1779 2031
Size: 1779 252
StyI C'CwwG_G
Cuts at: 0 1016 1155 2031
Size: 1016 139 876
Fragments arranged by size:
1016 876 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 196 2031
Size: 196 1835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 592 624 847 879 2031
Size: 55 32 505 32 223 32 1152
Fragments arranged by size:
1152 505 223 55 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1023 2031
Size: 1023 1008
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BfrBI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuII SacI SacII SanDI SexAI SfcI
SfoI SgrAI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaWI
BspEI BsrDI BsrGI BstBI BstEII BstXI BstZ17I ClaI
DraI DrdI EciI EcoRV FalI FseI FspAI HindIII
HpaI KpnI MluI NcoI NdeI NheI NotI NruI
PacI PciI PmeI PshAI PsiI PspXI PvuI RsrII
SalI SapI SbfI ScaI SfiI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI