Restriction map for
pF1KB3030
(Linear) (Six-base) MAPSORT of: KIBB3030.seq Check: 1141 from: 1 to: 2751
KIBB3030.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 1235 1247 1278 2751
Size: 1235 12 31 1473
Fragments arranged by size:
1473 1235 31 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1930 2751
Size: 1930 821
AflIII A'CryG_T
Cuts at: 0 1544 2751
Size: 1544 1207
AgeI A'CCGG_T
Cuts at: 0 991 2751
Size: 991 1760
AleI CACnn'nnGTG
Cuts at: 0 199 668 1543 2473 2716 2751
Size: 199 469 875 930 243 35
Fragments arranged by size:
930 875 469 243 199 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 35 1896 2751
Size: 35 1861 855
Fragments arranged by size:
1861 855 35
AlwNI CAG_nnn'CTG
Cuts at: 0 1452 2751
Size: 1452 1299
ApaLI G'TGCA_C
Cuts at: 0 2466 2751
Size: 2466 285
ApoI r'AATT_y
Cuts at: 0 1608 2170 2751
Size: 1608 562 581
Fragments arranged by size:
1608 581 562
AvaI C'yCGr_G
Cuts at: 0 861 1756 2205 2751
Size: 861 895 449 546
Fragments arranged by size:
895 861 546 449
BanI G'GyrC_C
Cuts at: 0 367 1302 1705 2751
Size: 367 935 403 1046
Fragments arranged by size:
1046 935 403 367
BanII G_rGCy'C
Cuts at: 0 41 305 853 1721 2751
Size: 41 264 548 868 1030
Fragments arranged by size:
1030 868 548 264 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 1420 2009 2751
Size: 1420 589 742
Fragments arranged by size:
1420 742 589
BciVI GTATCCnnnnn_n'
Cuts at: 0 2525 2751
Size: 2525 226
BclI T'GATC_A
Cuts at: 0 887 2231 2751
Size: 887 1344 520
Fragments arranged by size:
1344 887 520
BfrBI ATG'CAT
Cuts at: 0 2625 2751
Size: 2625 126
BglI GCCn_nnn'nGGC
Cuts at: 0 1168 2485 2751
Size: 1168 1317 266
Fragments arranged by size:
1317 1168 266
BglII A'GATC_T
Cuts at: 0 113 329 1673 2751
Size: 113 216 1344 1078
Fragments arranged by size:
1344 1078 216 113
BlpI GC'TnA_GC
Cuts at: 0 970 1435 2751
Size: 970 465 1316
Fragments arranged by size:
1316 970 465
Bme1580I G_kGCm'C
Cuts at: 0 694 1307 1407 1521 2470 2751
Size: 694 613 100 114 949 281
Fragments arranged by size:
949 694 613 281 114 100
BmgBI CAC'GTC
Cuts at: 0 1896 2751
Size: 1896 855
BmrI ACTGGGnnnn_n'
Cuts at: 0 457 2751
Size: 457 2294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 227 2751
Size: 227 2524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 289 1474 2751
Size: 197 92 1185 1277
Fragments arranged by size:
1277 1185 197 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1834 2311 2446 2751
Size: 1834 477 135 305
Fragments arranged by size:
1834 477 305 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 510 685 997 2751
Size: 94 416 175 312 1754
Fragments arranged by size:
1754 416 312 175 94
BsaAI yAC'GTr
Cuts at: 0 1545 2751
Size: 1545 1206
BsaBI GATnn'nnATC
Cuts at: 0 766 2751
Size: 766 1985
BsaWI w'CCGG_w
Cuts at: 0 566 991 1046 1115 2751
Size: 566 425 55 69 1636
Fragments arranged by size:
1636 566 425 69 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 597 961 1222 1479 1837 1948 2077 2751
Size: 597 364 261 257 358 111 129 674
Fragments arranged by size:
674 597 364 358 261 257 129 111
BseYI C'CCAG_C
Cuts at: 0 371 2492 2751
Size: 371 2121 259
Fragments arranged by size:
2121 371 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1376 2751
Size: 76 1300 1375
Fragments arranged by size:
1375 1300 76
BsiEI CG_ry'CG
Cuts at: 0 996 2751
Size: 996 1755
BsiHKAI G_wGCw'C
Cuts at: 0 41 1721 2434 2470 2751
Size: 41 1680 713 36 281
Fragments arranged by size:
1680 713 281 41 36
BsmI GAATG_Cn'
Cuts at: 0 400 2751
Size: 400 2351
BsmBI CGTCTCn'nnnn_
Cuts at: 0 542 2751
Size: 542 2209
Bsp1286I G_dGCh'C
Cuts at: 0 41 305 694 853 1307 1407 1521 1721
Size: 41 264 389 159 454 100 114 200
Cuts at: 1721 2434 2470 2751
Size: 713 36 281
Fragments arranged by size:
713 454 389 281 264 200 159 114
100 41 36
BspEI T'CCGG_A
Cuts at: 0 566 1046 2751
Size: 566 480 1705
Fragments arranged by size:
1705 566 480
BsrBI CCG'CTC
Cuts at: 0 1212 1331 2322 2751
Size: 1212 119 991 429
Fragments arranged by size:
1212 991 429 119
BsrDI GCAATG_nn'
Cuts at: 0 386 2052 2079 2751
Size: 386 1666 27 672
Fragments arranged by size:
1666 672 386 27
BsrFI r'CCGG_y
Cuts at: 0 991 1382 1925 2751
Size: 991 391 543 826
Fragments arranged by size:
991 826 543 391
BsrGI T'GTAC_A
Cuts at: 0 899 2751
Size: 899 1852
BstAPI GCAn_nnn'nTGC
Cuts at: 0 707 2051 2751
Size: 707 1344 700
Fragments arranged by size:
1344 707 700
BstEII G'GTnAC_C
Cuts at: 0 1899 2751
Size: 1899 852
BstXI CCAn_nnnn'nTGG
Cuts at: 0 537 2500 2751
Size: 537 1963 251
Fragments arranged by size:
1963 537 251
BstYI r'GATC_y
Cuts at: 0 113 249 329 1118 1673 2751
Size: 113 136 80 789 555 1078
Fragments arranged by size:
1078 789 555 136 113 80
Bsu36I CC'TnA_GG
Cuts at: 0 344 2751
Size: 344 2407
BtgI C'CryG_G
Cuts at: 0 1361 2544 2751
Size: 1361 1183 207
Fragments arranged by size:
1361 1183 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 764 1328 1728 2751
Size: 764 564 400 1023
Fragments arranged by size:
1023 764 564 400
BtsI GCAGTG_nn'
Cuts at: 0 185 369 1350 1379 2711 2751
Size: 185 184 981 29 1332 40
Fragments arranged by size:
1332 981 185 184 40 29
ClaI AT'CG_AT
Cuts at: 0 2276 2751
Size: 2276 475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 513 548 2751
Size: 513 35 2203
Fragments arranged by size:
2203 513 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1243 1732 2751
Size: 1243 489 1019
Fragments arranged by size:
1243 1019 489
EaeI y'GGCC_r
Cuts at: 0 938 1106 1169 1388 2513 2751
Size: 938 168 63 219 1125 238
Fragments arranged by size:
1125 938 238 219 168 63
EarI CTCTTCn'nnn_
Cuts at: 0 713 2090 2305 2751
Size: 713 1377 215 446
Fragments arranged by size:
1377 713 446 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 774 1134 2118 2751
Size: 774 360 984 633
Fragments arranged by size:
984 774 633 360
EcoICRI GAG'CTC
Cuts at: 0 39 1719 2751
Size: 39 1680 1032
Fragments arranged by size:
1680 1032 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 289 1474 1930 2751
Size: 197 92 1185 456 821
Fragments arranged by size:
1185 821 456 197 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 201 1909 2751
Size: 201 1708 842
Fragments arranged by size:
1708 842 201
EcoO109I rG'GnC_Cy
Cuts at: 0 785 1062 1617 2129 2585 2640 2751
Size: 785 277 555 512 456 55 111
Fragments arranged by size:
785 555 512 456 277 111 55
EcoRV GAT'ATC
Cuts at: 0 600 2751
Size: 600 2151
FspI TGC'GCA
Cuts at: 0 1158 2071 2751
Size: 1158 913 680
Fragments arranged by size:
1158 913 680
HaeII r_GCGC'y
Cuts at: 0 2707 2751
Size: 2707 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 309 341 787 819 835 867 2297 2329
Size: 309 32 446 32 16 32 1430 32
Cuts at: 2329 2751
Size: 422
Fragments arranged by size:
1430 446 422 309 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 66 1236 2751
Size: 66 1170 1515
Fragments arranged by size:
1515 1170 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1956 2751
Size: 1956 795
MscI TGG'CCA
Cuts at: 0 940 1108 2751
Size: 940 168 1643
Fragments arranged by size:
1643 940 168
MslI CAynn'nnrTG
Cuts at: 0 199 668 736 1514 1543 1768 2473 2716
Size: 199 469 68 778 29 225 705 243
Cuts at: 2716 2751
Size: 35
Fragments arranged by size:
778 705 469 243 225 199 68 35
29
MspA1I CmG'CkG
Cuts at: 0 375 1434 1449 2518 2690 2751
Size: 375 1059 15 1069 172 61
Fragments arranged by size:
1069 1059 375 172 61 15
NcoI C'CATG_G
Cuts at: 0 1361 2751
Size: 1361 1390
NsiI A_TGCA'T
Cuts at: 0 2627 2751
Size: 2627 124
NspI r_CATG'y
Cuts at: 0 87 2751
Size: 87 2664
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 1452 2751
Size: 312 1140 1299
Fragments arranged by size:
1299 1140 312
PpuMI rG'GwC_Cy
Cuts at: 0 1062 1617 2585 2640 2751
Size: 1062 555 968 55 111
Fragments arranged by size:
1062 968 555 111 55
PspXI vC'TCGA_Gb
Cuts at: 0 1756 2751
Size: 1756 995
PstI C_TGCA'G
Cuts at: 0 177 1184 1433 2675 2751
Size: 177 1007 249 1242 76
Fragments arranged by size:
1242 1007 249 177 76
PvuII CAG'CTG
Cuts at: 0 375 1434 1449 2751
Size: 375 1059 15 1302
Fragments arranged by size:
1302 1059 375 15
SacI G_AGCT'C
Cuts at: 0 41 1721 2751
Size: 41 1680 1030
Fragments arranged by size:
1680 1030 41
SalI G'TCGA_C
Cuts at: 0 1234 2751
Size: 1234 1517
SanDI GG'GwC_CC
Cuts at: 0 2585 2751
Size: 2585 166
SapI GCTCTTCn'nnn_
Cuts at: 0 2305 2751
Size: 2305 446
SfcI C'TryA_G
Cuts at: 0 173 1180 1429 2671 2751
Size: 173 1007 249 1242 80
Fragments arranged by size:
1242 1007 249 173 80
SgrAI Cr'CCGG_yG
Cuts at: 0 1925 2751
Size: 1925 826
SmaI CCC'GGG
Cuts at: 0 863 2207 2751
Size: 863 1344 544
Fragments arranged by size:
1344 863 544
SmlI C'TyrA_G
Cuts at: 0 1756 1849 2326 2425 2751
Size: 1756 93 477 99 326
Fragments arranged by size:
1756 477 326 99 93
StyI C'CwwG_G
Cuts at: 0 515 941 1361 1589 1596 2751
Size: 515 426 420 228 7 1155
Fragments arranged by size:
1155 515 426 420 228 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 270 385 2239 2563 2751
Size: 270 115 1854 324 188
Fragments arranged by size:
1854 324 270 188 115
TatI w'GTAC_w
Cuts at: 0 899 1760 2751
Size: 899 861 991
Fragments arranged by size:
991 899 861
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1041 1073 1635 1667 2751
Size: 1041 32 562 32 1084
Fragments arranged by size:
1084 1041 562 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1972 2751
Size: 1972 779
XhoI C'TCGA_G
Cuts at: 0 1756 2751
Size: 1756 995
XmaI C'CCGG_G
Cuts at: 0 861 2205 2751
Size: 861 1344 546
Fragments arranged by size:
1344 861 546
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NcoI NsiI NspI
PflMI PpuMI PspXI PstI PvuII SacI SalI SanDI
SapI SfcI SgrAI SmaI SmlI StyI TaqII TatI
TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BmtI Bpu10I BsaHI BsaXI BsiWI BspHI
BspMI BssHII BssSI BstBI BstZ17I DraI DraIII EagI
EcoRI FalI FseI FspAI HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XcmI XmnI ZraI