Restriction map for
pF1KB3037
(Linear) (Six-base) MAPSORT of: KIBB3037.seq Check: 3836 from: 1 to: 3450
KIBB3037.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 638 1940 2165 2449 2921 3145 3450
Size: 266 372 1302 225 284 472 224 305
Fragments arranged by size:
1302 472 372 305 284 266 225 224
AgeI A'CCGG_T
Cuts at: 0 1639 3260 3450
Size: 1639 1621 190
Fragments arranged by size:
1639 1621 190
AleI CACnn'nnGTG
Cuts at: 0 146 3450
Size: 146 3304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2641 3450
Size: 2641 809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 577 609 3450
Size: 499 32 46 32 2841
Fragments arranged by size:
2841 499 46 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1027 3450
Size: 1027 2423
ApoI r'AATT_y
Cuts at: 0 376 883 1122 1348 1471 1503 1868 2398
Size: 376 507 239 226 123 32 365 530
Cuts at: 2398 2518 2716 3370 3450
Size: 120 198 654 80
Fragments arranged by size:
654 530 507 376 365 239 226 198
123 120 80 32
BanII G_rGCy'C
Cuts at: 0 1767 3044 3248 3450
Size: 1767 1277 204 202
Fragments arranged by size:
1767 1277 204 202
BbsI GAAGACnn'nnnn_
Cuts at: 0 629 1454 1802 2326 2401 2912 3450
Size: 629 825 348 524 75 511 538
Fragments arranged by size:
825 629 538 524 511 348 75
BbvCI CC'TCA_GC
Cuts at: 0 3272 3450
Size: 3272 178
BciVI GTATCCnnnnn_n'
Cuts at: 0 573 2472 3450
Size: 573 1899 978
Fragments arranged by size:
1899 978 573
BclI T'GATC_A
Cuts at: 0 929 1988 2736 3450
Size: 929 1059 748 714
Fragments arranged by size:
1059 929 748 714
BfrBI ATG'CAT
Cuts at: 0 758 3450
Size: 758 2692
BglII A'GATC_T
Cuts at: 0 198 1656 2575 3120 3450
Size: 198 1458 919 545 330
Fragments arranged by size:
1458 919 545 330 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 964 3264 3450
Size: 964 2300 186
Fragments arranged by size:
2300 964 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1964 2024 2498 3010 3193 3450
Size: 1964 60 474 512 183 257
Fragments arranged by size:
1964 512 474 257 183 60
Bpu10I CC'TnA_GC
Cuts at: 0 3242 3272 3450
Size: 3242 30 178
Fragments arranged by size:
3242 178 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1681 1790 3450
Size: 1681 109 1660
Fragments arranged by size:
1681 1660 109
BsaI GGTCTCn'nnnn_
Cuts at: 0 1478 1564 2497 3450
Size: 1478 86 933 953
Fragments arranged by size:
1478 953 933 86
BsaBI GATnn'nnATC
Cuts at: 0 197 2735 3450
Size: 197 2538 715
Fragments arranged by size:
2538 715 197
BsaWI w'CCGG_w
Cuts at: 0 1639 3260 3450
Size: 1639 1621 190
Fragments arranged by size:
1639 1621 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2954 2984 3450
Size: 2954 30 466
Fragments arranged by size:
2954 466 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3019 3289 3450
Size: 3019 270 161
Fragments arranged by size:
3019 270 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 2846 3153 3450
Size: 176 2670 307 297
Fragments arranged by size:
2670 307 297 176
BsmI GAATG_Cn'
Cuts at: 0 1825 2109 3450
Size: 1825 284 1341
Fragments arranged by size:
1825 1341 284
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2957 3277 3450
Size: 2957 320 173
Fragments arranged by size:
2957 320 173
Bsp1286I G_dGCh'C
Cuts at: 0 1767 3044 3248 3450
Size: 1767 1277 204 202
Fragments arranged by size:
1767 1277 204 202
BspHI T'CATG_A
Cuts at: 0 1314 2172 2382 3450
Size: 1314 858 210 1068
Fragments arranged by size:
1314 1068 858 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 109 3363 3450
Size: 109 3254 87
Fragments arranged by size:
3254 109 87
BsrDI GCAATG_nn'
Cuts at: 0 2348 3450
Size: 2348 1102
BsrFI r'CCGG_y
Cuts at: 0 62 1639 3260 3450
Size: 62 1577 1621 190
Fragments arranged by size:
1621 1577 190 62
BsrGI T'GTAC_A
Cuts at: 0 1095 3450
Size: 1095 2355
BstXI CCAn_nnnn'nTGG
Cuts at: 0 229 3450
Size: 229 3221
BstYI r'GATC_y
Cuts at: 0 198 298 1656 2575 3120 3201 3450
Size: 198 100 1358 919 545 81 249
Fragments arranged by size:
1358 919 545 249 198 100 81
Bsu36I CC'TnA_GG
Cuts at: 0 1442 3450
Size: 1442 2008
BtgI C'CryG_G
Cuts at: 0 2128 2540 3029 3450
Size: 2128 412 489 421
Fragments arranged by size:
2128 489 421 412
BtsI GCAGTG_nn'
Cuts at: 0 3016 3450
Size: 3016 434
DraI TTT'AAA
Cuts at: 0 728 1619 3450
Size: 728 891 1831
Fragments arranged by size:
1831 891 728
DrdI GACnn_nn'nnGTC
Cuts at: 0 2340 3450
Size: 2340 1110
EaeI y'GGCC_r
Cuts at: 0 486 3450
Size: 486 2964
EarI CTCTTCn'nnn_
Cuts at: 0 1250 1472 1514 1686 1748 2645 3121 3450
Size: 1250 222 42 172 62 897 476 329
Fragments arranged by size:
1250 897 476 329 222 172 62 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 638 1940 1964 2024 2165 2449 2498
Size: 266 372 1302 24 60 141 284 49
Cuts at: 2498 2921 3010 3145 3193 3450
Size: 423 89 135 48 257
Fragments arranged by size:
1302 423 372 284 266 257 141 135
89 60 49 48 24
EcoRI G'AATT_C
Cuts at: 0 883 2518 3450
Size: 883 1635 932
Fragments arranged by size:
1635 932 883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 711 3450
Size: 711 2739
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 332 364 1441 1473 1534 1566
Size: 190 32 110 32 1077 32 61 32
Cuts at: 1566 1654 1686 2377 2409 2668 2700 3450
Size: 88 32 691 32 259 32 750
Fragments arranged by size:
1077 750 691 259 190 110 88 61
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1637 2571 3450
Size: 1637 934 879
Fragments arranged by size:
1637 934 879
HindIII A'AGCT_T
Cuts at: 0 3427 3450
Size: 3427 23
MscI TGG'CCA
Cuts at: 0 488 3450
Size: 488 2962
MslI CAynn'nnrTG
Cuts at: 0 146 175 227 688 1390 2353 2384 2387
Size: 146 29 52 461 702 963 31 3
Cuts at: 2387 2699 2953 3450
Size: 312 254 497
Fragments arranged by size:
963 702 497 461 312 254 146 52
31 29 3
MspA1I CmG'CkG
Cuts at: 0 2945 3094 3271 3450
Size: 2945 149 177 179
Fragments arranged by size:
2945 179 177 149
NcoI C'CATG_G
Cuts at: 0 3029 3450
Size: 3029 421
NsiI A_TGCA'T
Cuts at: 0 760 3450
Size: 760 2690
NspI r_CATG'y
Cuts at: 0 1363 3450
Size: 1363 2087
PasI CC'CwG_GG
Cuts at: 0 284 3450
Size: 284 3166
PflMI CCAn_nnn'nTGG
Cuts at: 0 520 976 3450
Size: 520 456 2474
Fragments arranged by size:
2474 520 456
PfoI T'CCnGG_A
Cuts at: 0 1781 3320 3450
Size: 1781 1539 130
Fragments arranged by size:
1781 1539 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2923 2955 3450
Size: 2923 32 495
Fragments arranged by size:
2923 495 32
PsiI TTA'TAA
Cuts at: 0 494 3450
Size: 494 2956
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 65 97 3450
Size: 65 32 3353
Fragments arranged by size:
3353 65 32
PstI C_TGCA'G
Cuts at: 0 2310 2431 3450
Size: 2310 121 1019
Fragments arranged by size:
2310 1019 121
PvuII CAG'CTG
Cuts at: 0 3094 3450
Size: 3094 356
ScaI AGT'ACT
Cuts at: 0 30 2995 3450
Size: 30 2965 455
Fragments arranged by size:
2965 455 30
SexAI A'CCwGG_T
Cuts at: 0 847 3450
Size: 847 2603
SfcI C'TryA_G
Cuts at: 0 42 1873 1917 2163 2306 2427 3450
Size: 42 1831 44 246 143 121 1023
Fragments arranged by size:
1831 1023 246 143 121 44 42
SmlI C'TyrA_G
Cuts at: 0 1660 1805 3450
Size: 1660 145 1645
Fragments arranged by size:
1660 1645 145
SpeI A'CTAG_T
Cuts at: 0 1812 3450
Size: 1812 1638
SspI AAT'ATT
Cuts at: 0 2558 3395 3450
Size: 2558 837 55
Fragments arranged by size:
2558 837 55
StuI AGG'CCT
Cuts at: 0 268 3450
Size: 268 3182
StyI C'CwwG_G
Cuts at: 0 3029 3450
Size: 3029 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1701 3450
Size: 1701 1749
TatI w'GTAC_w
Cuts at: 0 28 1095 2970 2993 3450
Size: 28 1067 1875 23 457
Fragments arranged by size:
1875 1067 457 28 23
Tth111I GACn'n_nGTC
Cuts at: 0 1310 3450
Size: 1310 2140
XbaI T'CTAG_A
Cuts at: 0 912 2436 3450
Size: 912 1524 1014
Fragments arranged by size:
1524 1014 912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2985 3450
Size: 2985 465
Enzymes that do cut:
AcuI AgeI AleI AlfI AloI AlwNI ApoI BanII
BbsI BbvCI BciVI BclI BfrBI BglII BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsgI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI
EarI Eco57MI EcoRI FalI Hin4I HincII HindIII MscI
MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI
PpiI PsiI PsrI PstI PvuII ScaI SexAI SfcI
SmlI SpeI SspI StuI StyI TaqII TatI Tth111I
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BglI BlpI Bme1580I
BmgBI BmtI BplI BsaAI BsaHI BseYI BsiEI BsiHKAI
BsiWI BspEI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraIII EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PmlI
PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI TstI XhoI XmaI
XmnI ZraI