Restriction map for
pF1KB3043
(Linear) (Six-base) MAPSORT of: KIBB3043.seq Check: 308 from: 1 to: 3456
KIBB3043.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 1796 2654 2774 3456
Size: 1796 858 120 682
Fragments arranged by size:
1796 858 682 120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 599 982 1047 2334 3456
Size: 106 493 383 65 1287 1122
Fragments arranged by size:
1287 1122 493 383 106 65
AfeI AGC'GCT
Cuts at: 0 1068 2512 3007 3456
Size: 1068 1444 495 449
Fragments arranged by size:
1444 1068 495 449
AflIII A'CryG_T
Cuts at: 0 257 347 932 3456
Size: 257 90 585 2524
Fragments arranged by size:
2524 585 257 90
AhdI GACnn_n'nnGTC
Cuts at: 0 38 3456
Size: 38 3418
AleI CACnn'nnGTG
Cuts at: 0 352 2560 3456
Size: 352 2208 896
Fragments arranged by size:
2208 896 352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1532 3456
Size: 1532 1924
AlwNI CAG_nnn'CTG
Cuts at: 0 306 1681 1758 1958 2413 3456
Size: 306 1375 77 200 455 1043
Fragments arranged by size:
1375 1043 455 306 200 77
ApaI G_GGCC'C
Cuts at: 0 1437 1626 2390 3456
Size: 1437 189 764 1066
Fragments arranged by size:
1437 1066 764 189
ApaLI G'TGCA_C
Cuts at: 0 325 3456
Size: 325 3131
ApoI r'AATT_y
Cuts at: 0 583 2740 3124 3456
Size: 583 2157 384 332
Fragments arranged by size:
2157 583 384 332
AvaI C'yCGr_G
Cuts at: 0 1457 2390 2982 3438 3456
Size: 1457 933 592 456 18
Fragments arranged by size:
1457 933 592 456 18
BamHI G'GATC_C
Cuts at: 0 127 379 3456
Size: 127 252 3077
Fragments arranged by size:
3077 252 127
BanI G'GyrC_C
Cuts at: 0 1266 1348 1360 2528 2916 3456
Size: 1266 82 12 1168 388 540
Fragments arranged by size:
1266 1168 540 388 82 12
BanII G_rGCy'C
Cuts at: 0 150 224 365 735 1437 1503 1626 1815
Size: 150 74 141 370 702 66 123 189
Cuts at: 1815 2182 2390 3456
Size: 367 208 1066
Fragments arranged by size:
1066 702 370 367 208 189 150 141
123 74 66
BbeI G_GCGC'C
Cuts at: 0 1364 2532 3456
Size: 1364 1168 924
Fragments arranged by size:
1364 1168 924
BbsI GAAGACnn'nnnn_
Cuts at: 0 970 2061 3456
Size: 970 1091 1395
Fragments arranged by size:
1395 1091 970
BbvCI CC'TCA_GC
Cuts at: 0 299 2338 3456
Size: 299 2039 1118
Fragments arranged by size:
2039 1118 299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1338 1372 3321 3355 3456
Size: 1338 34 1949 34 101
Fragments arranged by size:
1949 1338 101 34 34
BclI T'GATC_A
Cuts at: 0 927 1917 3456
Size: 927 990 1539
Fragments arranged by size:
1539 990 927
BglI GCCn_nnn'nGGC
Cuts at: 0 1621 2925 3456
Size: 1621 1304 531
Fragments arranged by size:
1621 1304 531
BglII A'GATC_T
Cuts at: 0 992 3212 3456
Size: 992 2220 244
Fragments arranged by size:
2220 992 244
BlpI GC'TnA_GC
Cuts at: 0 1096 3035 3456
Size: 1096 1939 421
Fragments arranged by size:
1939 1096 421
Bme1580I G_kGCm'C
Cuts at: 0 329 1269 1351 1437 1483 1626 1823 2390
Size: 329 940 82 86 46 143 197 567
Cuts at: 2390 2921 3456
Size: 531 535
Fragments arranged by size:
940 567 535 531 329 197 143 86
82 46
BmgBI CAC'GTC
Cuts at: 0 1395 1611 3456
Size: 1395 216 1845
Fragments arranged by size:
1845 1395 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 1128 2167 3456
Size: 1128 1039 1289
Fragments arranged by size:
1289 1128 1039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 534 1978 2565 2997 3456
Size: 534 1444 587 432 459
Fragments arranged by size:
1444 587 534 459 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1723 2485 2770 3456
Size: 1723 762 285 686
Fragments arranged by size:
1723 762 686 285
Bpu10I CC'TnA_GC
Cuts at: 0 299 1834 2338 3456
Size: 299 1535 504 1118
Fragments arranged by size:
1535 1118 504 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 2674 3456
Size: 419 2255 782
Fragments arranged by size:
2255 782 419
BsaI GGTCTCn'nnnn_
Cuts at: 0 2128 2805 3274 3456
Size: 2128 677 469 182
Fragments arranged by size:
2128 677 469 182
BsaAI yAC'GTr
Cuts at: 0 350 933 1824 2990 3456
Size: 350 583 891 1166 466
Fragments arranged by size:
1166 891 583 466 350
BsaHI Gr'CG_yC
Cuts at: 0 1361 2529 3456
Size: 1361 1168 927
Fragments arranged by size:
1361 1168 927
BsaWI w'CCGG_w
Cuts at: 0 587 2483 3456
Size: 587 1896 973
Fragments arranged by size:
1896 973 587
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3338 3368 3456
Size: 3338 30 88
Fragments arranged by size:
3338 88 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1648 2541 2677 2901 3456
Size: 1648 893 136 224 555
Fragments arranged by size:
1648 893 555 224 136
BseYI C'CCAG_C
Cuts at: 0 1578 1841 3456
Size: 1578 263 1615
Fragments arranged by size:
1615 1578 263
BsiEI CG_ry'CG
Cuts at: 0 1004 2049 3456
Size: 1004 1045 1407
Fragments arranged by size:
1407 1045 1004
BsiHKAI G_wGCw'C
Cuts at: 0 329 1102 1981 2584 3205 3456
Size: 329 773 879 603 621 251
Fragments arranged by size:
879 773 621 603 329 251
BsmI GAATG_Cn'
Cuts at: 0 747 3456
Size: 747 2709
Bsp1286I G_dGCh'C
Cuts at: 0 150 224 329 365 735 1102 1269 1351
Size: 150 74 105 36 370 367 167 82
Cuts at: 1351 1437 1483 1503 1626 1815 1823 1981 2182
Size: 86 46 20 123 189 8 158 201
Cuts at: 2182 2390 2584 2921 3205 3456
Size: 208 194 337 284 251
Fragments arranged by size:
370 367 337 284 251 208 201 194
189 167 158 150 123 105 86 82
74 46 36 20 8
BspEI T'CCGG_A
Cuts at: 0 587 3456
Size: 587 2869
BspHI T'CATG_A
Cuts at: 0 2357 3456
Size: 2357 1099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 225 3456
Size: 225 3231
BsrBI CCG'CTC
Cuts at: 0 3321 3456
Size: 3321 135
BsrDI GCAATG_nn'
Cuts at: 0 77 449 2319 3456
Size: 77 372 1870 1137
Fragments arranged by size:
1870 1137 372 77
BsrFI r'CCGG_y
Cuts at: 0 2757 3456
Size: 2757 699
BsrGI T'GTAC_A
Cuts at: 0 3380 3456
Size: 3380 76
BssSI C'ACGA_G
Cuts at: 0 923 2374 3456
Size: 923 1451 1082
Fragments arranged by size:
1451 1082 923
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1827 2588 3456
Size: 1827 761 868
Fragments arranged by size:
1827 868 761
BstEII G'GTnAC_C
Cuts at: 0 2451 2628 2784 2961 3456
Size: 2451 177 156 177 495
Fragments arranged by size:
2451 495 177 177 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 1747 1890 2904 3456
Size: 138 1609 143 1014 552
Fragments arranged by size:
1609 1014 552 143 138
BstYI r'GATC_y
Cuts at: 0 127 379 752 992 1678 2006 3212 3456
Size: 127 252 373 240 686 328 1206 244
Fragments arranged by size:
1206 686 373 328 252 244 240 127
Bsu36I CC'TnA_GG
Cuts at: 0 818 1674 3456
Size: 818 856 1782
Fragments arranged by size:
1782 856 818
BtgI C'CryG_G
Cuts at: 0 242 683 1388 2793 3053 3456
Size: 242 441 705 1405 260 403
Fragments arranged by size:
1405 705 441 403 260 242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 891 1312 3054 3456
Size: 891 421 1742 402
Fragments arranged by size:
1742 891 421 402
BtsI GCAGTG_nn'
Cuts at: 0 345 882 1516 3456
Size: 345 537 634 1940
Fragments arranged by size:
1940 634 537 345
DraIII CAC_nnn'GTG
Cuts at: 0 1762 3456
Size: 1762 1694
EaeI y'GGCC_r
Cuts at: 0 680 1613 2046 2245 3456
Size: 680 933 433 199 1211
Fragments arranged by size:
1211 933 680 433 199
EagI C'GGCC_G
Cuts at: 0 2046 3456
Size: 2046 1410
EarI CTCTTCn'nnn_
Cuts at: 0 1969 2281 2572 3456
Size: 1969 312 291 884
Fragments arranged by size:
1969 884 312 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 217 516 684 2272 3456
Size: 217 299 168 1588 1184
Fragments arranged by size:
1588 1184 299 217 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 599 982 1047 1723 2334 2485 2770
Size: 106 493 383 65 676 611 151 285
Cuts at: 2770 3456
Size: 686
Fragments arranged by size:
686 676 611 493 383 285 151 106
65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1488 3456
Size: 1488 1968
EcoO109I rG'GnC_Cy
Cuts at: 0 237 417 1373 1433 1434 1622 1623 1773
Size: 237 180 956 60 1 188 1 150
Cuts at: 1773 1838 2169 2387 2570 3048 3269 3303 3433
Size: 65 331 218 183 478 221 34 130
Cuts at: 3433 3456
Size: 23
Fragments arranged by size:
956 478 331 237 221 218 188 183
180 150 130 65 60 34 23 1
1
EcoRI G'AATT_C
Cuts at: 0 583 2740 3124 3456
Size: 583 2157 384 332
Fragments arranged by size:
2157 583 384 332
EcoRV GAT'ATC
Cuts at: 0 1276 3456
Size: 1276 2180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 762 3024 3408 3456
Size: 762 2262 384 48
Fragments arranged by size:
2262 762 384 48
HaeII r_GCGC'y
Cuts at: 0 1070 1364 2514 2532 3009 3456
Size: 1070 294 1150 18 477 447
Fragments arranged by size:
1150 1070 477 447 294 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 821 853 1670 1702 2641 2673
Size: 24 32 765 32 817 32 939 32
Cuts at: 2673 3338 3370 3456
Size: 665 32 86
Fragments arranged by size:
939 817 765 665 86 32 32 32
32 32 24
HincII GTy'rAC
Cuts at: 0 23 3456
Size: 23 3433
HpaI GTT'AAC
Cuts at: 0 23 3456
Size: 23 3433
KasI G'GCGC_C
Cuts at: 0 1360 2528 3456
Size: 1360 1168 928
Fragments arranged by size:
1360 1168 928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 407 1355 2769 3047 3456
Size: 123 284 948 1414 278 409
Fragments arranged by size:
1414 948 409 284 278 123
MscI TGG'CCA
Cuts at: 0 1615 3456
Size: 1615 1841
MslI CAynn'nnrTG
Cuts at: 0 352 856 1171 1318 2560 3456
Size: 352 504 315 147 1242 896
Fragments arranged by size:
1242 896 504 352 315 147
MspA1I CmG'CkG
Cuts at: 0 303 660 1707 2588 3208 3456
Size: 303 357 1047 881 620 248
Fragments arranged by size:
1047 881 620 357 303 248
NarI GG'CG_CC
Cuts at: 0 1361 2529 3456
Size: 1361 1168 927
Fragments arranged by size:
1361 1168 927
NcoI C'CATG_G
Cuts at: 0 2793 3456
Size: 2793 663
NdeI CA'TA_TG
Cuts at: 0 2944 3456
Size: 2944 512
NspI r_CATG'y
Cuts at: 0 261 1323 2721 3456
Size: 261 1062 1398 735
Fragments arranged by size:
1398 1062 735 261
PasI CC'CwG_GG
Cuts at: 0 264 1490 1547 1815 1883 3456
Size: 264 1226 57 268 68 1573
Fragments arranged by size:
1573 1226 268 264 68 57
PciI A'CATG_T
Cuts at: 0 257 3456
Size: 257 3199
PfoI T'CCnGG_A
Cuts at: 0 2612 3116 3456
Size: 2612 504 340
Fragments arranged by size:
2612 504 340
PmlI CAC'GTG
Cuts at: 0 933 1824 3456
Size: 933 891 1632
Fragments arranged by size:
1632 933 891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2497 2529 3456
Size: 2497 32 927
Fragments arranged by size:
2497 927 32
PpuMI rG'GwC_Cy
Cuts at: 0 237 1773 1838 2169 3269 3303 3433 3456
Size: 237 1536 65 331 1100 34 130 23
Fragments arranged by size:
1536 1100 331 237 130 65 34 23
PshAI GACnn'nnGTC
Cuts at: 0 2107 2449 3456
Size: 2107 342 1007
Fragments arranged by size:
2107 1007 342
PspOMI G'GGCC_C
Cuts at: 0 1433 1622 2386 3456
Size: 1433 189 764 1070
Fragments arranged by size:
1433 1070 764 189
PstI C_TGCA'G
Cuts at: 0 236 2593 3456
Size: 236 2357 863
Fragments arranged by size:
2357 863 236
PvuI CG_AT'CG
Cuts at: 0 1004 3456
Size: 1004 2452
PvuII CAG'CTG
Cuts at: 0 303 660 2588 3208 3456
Size: 303 357 1928 620 248
Fragments arranged by size:
1928 620 357 303 248
SanDI GG'GwC_CC
Cuts at: 0 3433 3456
Size: 3433 23
SapI GCTCTTCn'nnn_
Cuts at: 0 1969 2572 3456
Size: 1969 603 884
Fragments arranged by size:
1969 884 603
SbfI CC_TGCA'GG
Cuts at: 0 236 3456
Size: 236 3220
ScaI AGT'ACT
Cuts at: 0 574 1850 3456
Size: 574 1276 1606
Fragments arranged by size:
1606 1276 574
SexAI A'CCwGG_T
Cuts at: 0 1418 3456
Size: 1418 2038
SfcI C'TryA_G
Cuts at: 0 201 232 1805 2146 2439 2589 3456
Size: 201 31 1573 341 293 150 867
Fragments arranged by size:
1573 867 341 293 201 150 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1621 3456
Size: 1621 1835
SfoI GGC'GCC
Cuts at: 0 1362 2530 3456
Size: 1362 1168 926
Fragments arranged by size:
1362 1168 926
SmaI CCC'GGG
Cuts at: 0 2392 3440 3456
Size: 2392 1048 16
Fragments arranged by size:
2392 1048 16
SmlI C'TyrA_G
Cuts at: 0 434 2689 2982 3456
Size: 434 2255 293 474
Fragments arranged by size:
2255 474 434 293
StyI C'CwwG_G
Cuts at: 0 794 2670 2793 2999 3456
Size: 794 1876 123 206 457
Fragments arranged by size:
1876 794 457 206 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1366 2371 3456
Size: 1366 1005 1085
Fragments arranged by size:
1366 1085 1005
TatI w'GTAC_w
Cuts at: 0 572 1848 3380 3456
Size: 572 1276 1532 76
Fragments arranged by size:
1532 1276 572 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 119 151 3456
Size: 119 32 3305
Fragments arranged by size:
3305 119 32
Tth111I GACn'n_nGTC
Cuts at: 0 1420 3456
Size: 1420 2036
XhoI C'TCGA_G
Cuts at: 0 2982 3456
Size: 2982 474
XmaI C'CCGG_G
Cuts at: 0 2390 3438 3456
Size: 2390 1048 18
Fragments arranged by size:
2390 1048 18
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI
KasI MmeI MscI MslI MspA1I NarI NcoI NdeI
NspI PasI PciI PfoI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI StyI TaqII
TatI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AloI AscI
AseI AsiSI AvrII BaeI BciVI BfrBI BmtI BsaBI
BsgI BsiWI BsmBI BssHII BstBI BstZ17I ClaI CspCI
DraI DrdI EcoICRI FseI FspI FspAI HindIII KpnI
MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PsiI PspXI PsrI RsrII SacI
SacII SalI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XcmI XmnI ZraI