Restriction map for
pF1KB3044
(Linear) (Six-base) MAPSORT of: KIBB3044.seq Check: 4909 from: 1 to: 1872
KIBB3044.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 384 516 1286 1872
Size: 384 132 770 586
Fragments arranged by size:
770 586 384 132
AclI AA'CG_TT
Cuts at: 0 1821 1872
Size: 1821 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1872
Size: 191 1681
AfeI AGC'GCT
Cuts at: 0 76 1872
Size: 76 1796
AflII C'TTAA_G
Cuts at: 0 892 1872
Size: 892 980
AflIII A'CryG_T
Cuts at: 0 628 687 1872
Size: 628 59 1185
Fragments arranged by size:
1185 628 59
AhdI GACnn_n'nnGTC
Cuts at: 0 1369 1872
Size: 1369 503
ApoI r'AATT_y
Cuts at: 0 1810 1872
Size: 1810 62
AseI AT'TA_AT
Cuts at: 0 707 950 1872
Size: 707 243 922
Fragments arranged by size:
922 707 243
BamHI G'GATC_C
Cuts at: 0 42 199 300 1323 1872
Size: 42 157 101 1023 549
Fragments arranged by size:
1023 549 157 101 42
BanI G'GyrC_C
Cuts at: 0 1764 1872
Size: 1764 108
BanII G_rGCy'C
Cuts at: 0 34 1872
Size: 34 1838
BbsI GAAGACnn'nnnn_
Cuts at: 0 1127 1872
Size: 1127 745
BciVI GTATCCnnnnn_n'
Cuts at: 0 1814 1872
Size: 1814 58
Bme1580I G_kGCm'C
Cuts at: 0 1767 1872
Size: 1767 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 1419 1872
Size: 1419 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1085 1775 1872
Size: 434 651 690 97
Fragments arranged by size:
690 651 434 97
Bpu10I CC'TnA_GC
Cuts at: 0 296 1872
Size: 296 1576
BsaAI yAC'GTr
Cuts at: 0 472 1872
Size: 472 1400
BsaBI GATnn'nnATC
Cuts at: 0 305 363 369 1872
Size: 305 58 6 1503
Fragments arranged by size:
1503 305 58 6
BsaHI Gr'CG_yC
Cuts at: 0 1355 1872
Size: 1355 517
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 1604 1872
Size: 52 1552 268
Fragments arranged by size:
1552 268 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1872
Size: 1222 650
BsiHKAI G_wGCw'C
Cuts at: 0 297 1614 1872
Size: 297 1317 258
Fragments arranged by size:
1317 297 258
BsmBI CGTCTCn'nnnn_
Cuts at: 0 43 1505 1872
Size: 43 1462 367
Fragments arranged by size:
1462 367 43
Bsp1286I G_dGCh'C
Cuts at: 0 34 297 1614 1767 1872
Size: 34 263 1317 153 105
Fragments arranged by size:
1317 263 153 105 34
BspHI T'CATG_A
Cuts at: 0 1602 1872
Size: 1602 270
BsrDI GCAATG_nn'
Cuts at: 0 325 887 1410 1872
Size: 325 562 523 462
Fragments arranged by size:
562 523 462 325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 754 1872
Size: 754 1118
BstBI TT'CG_AA
Cuts at: 0 1536 1872
Size: 1536 336
BstYI r'GATC_y
Cuts at: 0 42 199 300 364 1323 1872
Size: 42 157 101 64 959 549
Fragments arranged by size:
959 549 157 101 64 42
BstZ17I GTA'TAC
Cuts at: 0 1287 1872
Size: 1287 585
BtsI GCAGTG_nn'
Cuts at: 0 1793 1872
Size: 1793 79
DraI TTT'AAA
Cuts at: 0 723 744 1331 1872
Size: 723 21 587 541
Fragments arranged by size:
723 587 541 21
DraIII CAC_nnn'GTG
Cuts at: 0 1024 1872
Size: 1024 848
EarI CTCTTCn'nnn_
Cuts at: 0 450 817 953 1128 1187 1287 1872
Size: 450 367 136 175 59 100 585
Fragments arranged by size:
585 450 367 175 136 100 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1253 1872
Size: 1253 619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 434 1085 1775 1872
Size: 191 243 651 690 97
Fragments arranged by size:
690 651 243 191 97
EcoO109I rG'GnC_Cy
Cuts at: 0 84 934 1872
Size: 84 850 938
Fragments arranged by size:
938 850 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1003 1872
Size: 1003 869
HaeII r_GCGC'y
Cuts at: 0 78 1872
Size: 78 1794
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 188 220 550 582 1355 1387
Size: 40 32 116 32 330 32 773 32
Cuts at: 1387 1872
Size: 485
Fragments arranged by size:
773 485 330 116 40 32 32 32
32
HincII GTy'rAC
Cuts at: 0 385 525 1395 1872
Size: 385 140 870 477
Fragments arranged by size:
870 477 385 140
MfeI C'AATT_G
Cuts at: 0 1248 1872
Size: 1248 624
MluI A'CGCG_T
Cuts at: 0 628 1872
Size: 628 1244
MslI CAynn'nnrTG
Cuts at: 0 1607 1872
Size: 1607 265
MspA1I CmG'CkG
Cuts at: 0 815 1872
Size: 815 1057
NdeI CA'TA_TG
Cuts at: 0 1734 1872
Size: 1734 138
NspI r_CATG'y
Cuts at: 0 691 1872
Size: 691 1181
PciI A'CATG_T
Cuts at: 0 687 1872
Size: 687 1185
PpuMI rG'GwC_Cy
Cuts at: 0 84 1872
Size: 84 1788
PstI C_TGCA'G
Cuts at: 0 196 1854 1872
Size: 196 1658 18
Fragments arranged by size:
1658 196 18
PvuII CAG'CTG
Cuts at: 0 815 1872
Size: 815 1057
SalI G'TCGA_C
Cuts at: 0 383 1872
Size: 383 1489
SapI GCTCTTCn'nnn_
Cuts at: 0 1128 1872
Size: 1128 744
ScaI AGT'ACT
Cuts at: 0 541 1872
Size: 541 1331
SfcI C'TryA_G
Cuts at: 0 192 532 1113 1421 1850 1872
Size: 192 340 581 308 429 22
Fragments arranged by size:
581 429 340 308 192 22
SmlI C'TyrA_G
Cuts at: 0 892 1872
Size: 892 980
StyI C'CwwG_G
Cuts at: 0 1670 1872
Size: 1670 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 104 1872
Size: 104 1768
TatI w'GTAC_w
Cuts at: 0 539 1872
Size: 539 1333
Tth111I GACn'n_nGTC
Cuts at: 0 54 1872
Size: 54 1818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1254 1872
Size: 1254 618
XmnI GAAnn'nnTTC
Cuts at: 0 1430 1872
Size: 1430 442
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AhdI ApoI
AseI BamHI BanI BanII BbsI BciVI Bme1580I BmrI
BpmI Bpu10I BsaAI BsaBI BsaHI BseRI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BsrDI BstAPI BstBI BstYI BstZ17I
BtsI DraI DraIII EarI EciI Eco57MI EcoO109I FalI
HaeII Hin4I HincII MfeI MluI MslI MspA1I NdeI
NspI PciI PpuMI PstI PvuII SalI SapI ScaI
SfcI SmlI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpuEI BsaI BsaWI BsaXI BseYI BsiEI
BsiWI BsmI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstEII BstXI Bsu36I BtgI BtgZI ClaI CspCI
DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MmeI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XhoI XmaI ZraI