(Linear) (Six-base) MAPSORT of: KIBB3046.seq Check: 1740 from: 1 to: 4521 KIBB3046.orf With 182 enzymes: SgfI * July 18, 2007 13:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3607 4521 Size: 3607 914 AccI GT'mk_AC Cuts at: 0 689 1466 4521 Size: 689 777 3055 Fragments arranged by size: 3055 777 689 Acc65I G'GTAC_C Cuts at: 0 257 550 4521 Size: 257 293 3971 Fragments arranged by size: 3971 293 257 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 761 1613 1762 3337 4463 4521 Size: 82 679 852 149 1575 1126 58 Fragments arranged by size: 1575 1126 852 679 149 82 58 AflIII A'CryG_T Cuts at: 0 2566 2603 3603 4521 Size: 2566 37 1000 918 Fragments arranged by size: 2566 1000 918 37 AhdI GACnn_n'nnGTC Cuts at: 0 2604 4521 Size: 2604 1917 AleI CACnn'nnGTG Cuts at: 0 676 2000 3862 4240 4521 Size: 676 1324 1862 378 281 Fragments arranged by size: 1862 1324 676 378 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 4520 4521 Size: 4520 1 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2266 2298 2737 2769 4521 Size: 2266 32 439 32 1752 Fragments arranged by size: 2266 1752 439 32 32 ApaI G_GGCC'C Cuts at: 0 3540 4521 Size: 3540 981 ApaLI G'TGCA_C Cuts at: 0 745 4521 Size: 745 3776 ApoI r'AATT_y Cuts at: 0 109 571 615 1339 1877 2992 3370 3469 Size: 109 462 44 724 538 1115 378 99 Cuts at: 3469 3523 4351 4521 Size: 54 828 170 Fragments arranged by size: 1115 828 724 538 462 378 170 109 99 54 44 AvaI C'yCGr_G Cuts at: 0 1164 1274 3539 3627 4521 Size: 1164 110 2265 88 894 Fragments arranged by size: 2265 1164 894 110 88 AvrII C'CTAG_G Cuts at: 0 2247 4521 Size: 2247 2274 BamHI G'GATC_C Cuts at: 0 600 4521 Size: 600 3921 BanI G'GyrC_C Cuts at: 0 257 550 934 3330 4521 Size: 257 293 384 2396 1191 Fragments arranged by size: 2396 1191 384 293 257 BanII G_rGCy'C Cuts at: 0 339 2702 2789 3540 4521 Size: 339 2363 87 751 981 Fragments arranged by size: 2363 981 751 339 87 BbsI GAAGACnn'nnnn_ Cuts at: 0 70 1661 2296 3052 4521 Size: 70 1591 635 756 1469 Fragments arranged by size: 1591 1469 756 635 70 BbvCI CC'TCA_GC Cuts at: 0 626 1846 2812 3278 4521 Size: 626 1220 966 466 1243 Fragments arranged by size: 1243 1220 966 626 466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1706 1740 4441 4475 4521 Size: 1706 34 2701 34 46 Fragments arranged by size: 2701 1706 46 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1452 2292 3028 4521 Size: 1452 840 736 1493 Fragments arranged by size: 1493 1452 840 736 BfrBI ATG'CAT Cuts at: 0 1116 4521 Size: 1116 3405 BglI GCCn_nnn'nGGC Cuts at: 0 2093 4521 Size: 2093 2428 BglII A'GATC_T Cuts at: 0 2532 4521 Size: 2532 1989 Bme1580I G_kGCm'C Cuts at: 0 749 3253 3540 4521 Size: 749 2504 287 981 Fragments arranged by size: 2504 981 749 287 BmrI ACTGGGnnnn_n' Cuts at: 0 373 1762 1804 3502 4234 4521 Size: 373 1389 42 1698 732 287 Fragments arranged by size: 1698 1389 732 373 287 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1612 3303 3818 4521 Size: 1612 1691 515 703 Fragments arranged by size: 1691 1612 703 515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1355 4037 4521 Size: 1355 2682 484 Fragments arranged by size: 2682 1355 484 Bpu10I CC'TnA_GC Cuts at: 0 626 1809 1846 2812 3278 3326 4521 Size: 626 1183 37 966 466 48 1195 Fragments arranged by size: 1195 1183 966 626 466 48 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1343 1582 3813 4241 4521 Size: 1343 239 2231 428 280 Fragments arranged by size: 2231 1343 428 280 239 BsaBI GATnn'nnATC Cuts at: 0 799 4114 4521 Size: 799 3315 407 Fragments arranged by size: 3315 799 407 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 728 758 874 904 2737 2767 2800 2830 Size: 728 30 116 30 1833 30 33 30 Cuts at: 2830 3866 3896 4521 Size: 1036 30 625 Fragments arranged by size: 1833 1036 728 625 116 33 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 749 2452 4399 4521 Size: 749 1703 1947 122 Fragments arranged by size: 1947 1703 749 122 BseYI C'CCAG_C Cuts at: 0 822 2345 4273 4521 Size: 822 1523 1928 248 Fragments arranged by size: 1928 1523 822 248 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 1162 4471 4521 Size: 77 1085 3309 50 Fragments arranged by size: 3309 1085 77 50 BsiHKAI G_wGCw'C Cuts at: 0 339 749 1598 3085 3506 4521 Size: 339 410 849 1487 421 1015 Fragments arranged by size: 1487 1015 849 421 410 339 BsmI GAATG_Cn' Cuts at: 0 527 2113 2172 2224 4521 Size: 527 1586 59 52 2297 Fragments arranged by size: 2297 1586 527 59 52 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2055 2503 4259 4521 Size: 2055 448 1756 262 Fragments arranged by size: 2055 1756 448 262 Bsp1286I G_dGCh'C Cuts at: 0 339 749 1598 2702 2789 3085 3253 3506 Size: 339 410 849 1104 87 296 168 253 Cuts at: 3506 3540 4521 Size: 34 981 Fragments arranged by size: 1104 981 849 410 339 296 253 168 87 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3607 4521 Size: 3607 914 BsrDI GCAATG_nn' Cuts at: 0 478 1415 2228 2579 4073 4521 Size: 478 937 813 351 1494 448 Fragments arranged by size: 1494 937 813 478 448 351 BstAPI GCAn_nnn'nTGC Cuts at: 0 1267 2230 2242 4521 Size: 1267 963 12 2279 Fragments arranged by size: 2279 1267 963 12 BstEII G'GTnAC_C Cuts at: 0 1158 2328 4425 4521 Size: 1158 1170 2097 96 Fragments arranged by size: 2097 1170 1158 96 BstXI CCAn_nnnn'nTGG Cuts at: 0 678 1440 4242 4247 4521 Size: 678 762 2802 5 274 Fragments arranged by size: 2802 762 678 274 5 BstYI r'GATC_y Cuts at: 0 600 1354 2349 2532 4521 Size: 600 754 995 183 1989 Fragments arranged by size: 1989 995 754 600 183 BtgI C'CryG_G Cuts at: 0 956 1985 2940 4190 4521 Size: 956 1029 955 1250 331 Fragments arranged by size: 1250 1029 956 955 331 BtsI GCAGTG_nn' Cuts at: 0 1724 2991 3025 4521 Size: 1724 1267 34 1496 Fragments arranged by size: 1724 1496 1267 34 ClaI AT'CG_AT Cuts at: 0 1726 4521 Size: 1726 2795 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3365 3400 4521 Size: 3365 35 1121 Fragments arranged by size: 3365 1121 35 DraI TTT'AAA Cuts at: 0 2675 4521 Size: 2675 1846 DraIII CAC_nnn'GTG Cuts at: 0 1798 3510 4521 Size: 1798 1712 1011 Fragments arranged by size: 1798 1712 1011 DrdI GACnn_nn'nnGTC Cuts at: 0 1951 4521 Size: 1951 2570 EaeI y'GGCC_r Cuts at: 0 204 308 959 1548 2518 3854 4132 4249 Size: 204 104 651 589 970 1336 278 117 Cuts at: 4249 4521 Size: 272 Fragments arranged by size: 1336 970 651 589 278 272 204 117 104 EarI CTCTTCn'nnn_ Cuts at: 0 3113 3473 4287 4521 Size: 3113 360 814 234 Fragments arranged by size: 3113 814 360 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 706 1773 4521 Size: 706 1067 2748 Fragments arranged by size: 2748 1067 706 EcoICRI GAG'CTC Cuts at: 0 337 4521 Size: 337 4184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 761 1355 1613 1762 3337 4037 4463 Size: 82 679 594 258 149 1575 700 426 Cuts at: 4463 4521 Size: 58 Fragments arranged by size: 1575 700 679 594 426 258 149 82 58 EcoNI CCTnn'n_nnAGG Cuts at: 0 3478 4521 Size: 3478 1043 EcoO109I rG'GnC_Cy Cuts at: 0 3536 4521 Size: 3536 985 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 110 142 284 316 2590 2622 4521 Size: 110 32 142 32 2274 32 1899 Fragments arranged by size: 2274 1899 142 110 32 32 32 HincII GTy'rAC Cuts at: 0 1089 3034 3840 4521 Size: 1089 1945 806 681 Fragments arranged by size: 1945 1089 806 681 HindIII A'AGCT_T Cuts at: 0 2453 4521 Size: 2453 2068 KpnI G_GTAC'C Cuts at: 0 261 554 4521 Size: 261 293 3967 Fragments arranged by size: 3967 293 261 MfeI C'AATT_G Cuts at: 0 1947 4521 Size: 1947 2574 MluI A'CGCG_T Cuts at: 0 2566 4521 Size: 2566 1955 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1885 3168 3925 4521 Size: 1885 1283 757 596 Fragments arranged by size: 1885 1283 757 596 MscI TGG'CCA Cuts at: 0 206 310 961 1550 2520 3856 4521 Size: 206 104 651 589 970 1336 665 Fragments arranged by size: 1336 970 665 651 589 206 104 MslI CAynn'nnrTG Cuts at: 0 520 676 778 1034 2000 2161 3643 3850 Size: 520 156 102 256 966 161 1482 207 Cuts at: 3850 3862 4240 4521 Size: 12 378 281 Fragments arranged by size: 1482 966 520 378 281 256 207 161 156 102 12 MspA1I CmG'CkG Cuts at: 0 1665 2345 3301 4387 4521 Size: 1665 680 956 1086 134 Fragments arranged by size: 1665 1086 956 680 134 NcoI C'CATG_G Cuts at: 0 956 1985 2940 4521 Size: 956 1029 955 1581 Fragments arranged by size: 1581 1029 956 955 NsiI A_TGCA'T Cuts at: 0 1118 4521 Size: 1118 3403 NspI r_CATG'y Cuts at: 0 2607 3167 3607 4521 Size: 2607 560 440 914 Fragments arranged by size: 2607 914 560 440 PciI A'CATG_T Cuts at: 0 2603 3603 4521 Size: 2603 1000 918 Fragments arranged by size: 2603 1000 918 PflMI CCAn_nnn'nTGG Cuts at: 0 450 892 1881 4521 Size: 450 442 989 2640 Fragments arranged by size: 2640 989 450 442 PfoI T'CCnGG_A Cuts at: 0 167 2284 4521 Size: 167 2117 2237 Fragments arranged by size: 2237 2117 167 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2737 2769 4521 Size: 2737 32 1752 Fragments arranged by size: 2737 1752 32 PspOMI G'GGCC_C Cuts at: 0 3536 4521 Size: 3536 985 PstI C_TGCA'G Cuts at: 0 2868 3266 4521 Size: 2868 398 1255 Fragments arranged by size: 2868 1255 398 PvuII CAG'CTG Cuts at: 0 1665 3301 4387 4521 Size: 1665 1636 1086 134 Fragments arranged by size: 1665 1636 1086 134 SacI G_AGCT'C Cuts at: 0 339 4521 Size: 339 4182 SfcI C'TryA_G Cuts at: 0 478 686 2477 2864 3262 4491 4521 Size: 478 208 1791 387 398 1229 30 Fragments arranged by size: 1791 1229 478 398 387 208 30 SmaI CCC'GGG Cuts at: 0 1166 4521 Size: 1166 3355 SmlI C'TyrA_G Cuts at: 0 1358 1597 3792 4256 4521 Size: 1358 239 2195 464 265 Fragments arranged by size: 2195 1358 464 265 239 SpeI A'CTAG_T Cuts at: 0 1263 4521 Size: 1263 3258 SspI AAT'ATT Cuts at: 0 379 4521 Size: 379 4142 StuI AGG'CCT Cuts at: 0 1858 3408 4521 Size: 1858 1550 1113 Fragments arranged by size: 1858 1550 1113 StyI C'CwwG_G Cuts at: 0 638 956 1040 1985 2247 2585 2781 2836 Size: 638 318 84 945 262 338 196 55 Cuts at: 2836 2940 3128 3334 3669 3860 4143 4521 Size: 104 188 206 335 191 283 378 Fragments arranged by size: 945 638 378 338 335 318 283 262 206 196 191 188 104 84 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1166 1534 1786 1978 2092 2486 3521 4521 Size: 1166 368 252 192 114 394 1035 1000 Fragments arranged by size: 1166 1035 1000 394 368 252 192 114 TatI w'GTAC_w Cuts at: 0 901 3110 4499 4521 Size: 901 2209 1389 22 Fragments arranged by size: 2209 1389 901 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 149 181 728 760 2322 2354 2936 2968 Size: 149 32 547 32 1562 32 582 32 Cuts at: 2968 4521 Size: 1553 Fragments arranged by size: 1562 1553 582 547 149 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1878 4020 4521 Size: 1878 2142 501 Fragments arranged by size: 2142 1878 501 XmaI C'CCGG_G Cuts at: 0 1164 4521 Size: 1164 3357 XmnI GAAnn'nnTTC Cuts at: 0 491 1928 3469 4043 4521 Size: 491 1437 1541 574 478 Fragments arranged by size: 1541 1437 574 491 478 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI AloI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NsiI NspI PciI PflMI PfoI PpiI PspOMI PstI PvuII SacI SfcI SmaI SmlI SpeI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AlwNI AscI AseI AsiSI BaeI BbeI BclI BlpI BmgBI BmtI BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SwaI Tth111I XbaI XhoI ZraI