Restriction map for
pF1KB3051
(Linear) (Six-base) MAPSORT of: KIBB3051.seq Check: 2709 from: 1 to: 3645
KIBB3051.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1409 2307 3645
Size: 1409 898 1338
Fragments arranged by size:
1409 1338 898
AatII G_ACGT'C
Cuts at: 0 323 691 3645
Size: 323 368 2954
Fragments arranged by size:
2954 368 323
AccI GT'mk_AC
Cuts at: 0 1151 2626 3572 3645
Size: 1151 1475 946 73
Fragments arranged by size:
1475 1151 946 73
Acc65I G'GTAC_C
Cuts at: 0 1165 2075 3645
Size: 1165 910 1570
Fragments arranged by size:
1570 1165 910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 544 583 1012 1738 3282 3294 3645
Size: 160 384 39 429 726 1544 12 351
Fragments arranged by size:
1544 726 429 384 351 160 39 12
AfeI AGC'GCT
Cuts at: 0 1791 3645
Size: 1791 1854
AhdI GACnn_n'nnGTC
Cuts at: 0 1902 3645
Size: 1902 1743
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 3645
Size: 640 32 2973
Fragments arranged by size:
2973 640 32
AlwNI CAG_nnn'CTG
Cuts at: 0 138 1044 2208 3645
Size: 138 906 1164 1437
Fragments arranged by size:
1437 1164 906 138
ApaI G_GGCC'C
Cuts at: 0 1080 3645
Size: 1080 2565
ApaLI G'TGCA_C
Cuts at: 0 1394 3645
Size: 1394 2251
ApoI r'AATT_y
Cuts at: 0 2967 3645
Size: 2967 678
AscI GG'CGCG_CC
Cuts at: 0 2912 3645
Size: 2912 733
AvaI C'yCGr_G
Cuts at: 0 532 589 2088 3645
Size: 532 57 1499 1557
Fragments arranged by size:
1557 1499 532 57
BanI G'GyrC_C
Cuts at: 0 313 1165 2000 2075 2195 3645
Size: 313 852 835 75 120 1450
Fragments arranged by size:
1450 852 835 313 120 75
BanII G_rGCy'C
Cuts at: 0 785 1080 2056 2403 2687 3083 3130 3397
Size: 785 295 976 347 284 396 47 267
Cuts at: 3397 3645
Size: 248
Fragments arranged by size:
976 785 396 347 295 284 267 248
47
BbeI G_GCGC'C
Cuts at: 0 2004 3645
Size: 2004 1641
BbsI GAAGACnn'nnnn_
Cuts at: 0 1000 1923 3645
Size: 1000 923 1722
Fragments arranged by size:
1722 1000 923
BbvCI CC'TCA_GC
Cuts at: 0 2603 3645
Size: 2603 1042
BciVI GTATCCnnnnn_n'
Cuts at: 0 199 3645
Size: 199 3446
BclI T'GATC_A
Cuts at: 0 503 3329 3645
Size: 503 2826 316
Fragments arranged by size:
2826 503 316
BglI GCCn_nnn'nGGC
Cuts at: 0 1624 1828 3112 3645
Size: 1624 204 1284 533
Fragments arranged by size:
1624 1284 533 204
BglII A'GATC_T
Cuts at: 0 3446 3645
Size: 3446 199
BlpI GC'TnA_GC
Cuts at: 0 3082 3645
Size: 3082 563
Bme1580I G_kGCm'C
Cuts at: 0 1080 1291 1398 3645
Size: 1080 211 107 2247
Fragments arranged by size:
2247 1080 211 107
BmgBI CAC'GTC
Cuts at: 0 809 1329 1767 3645
Size: 809 520 438 1878
Fragments arranged by size:
1878 809 520 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 714 1668 1810 3186 3645
Size: 714 954 142 1376 459
Fragments arranged by size:
1376 954 714 459 142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1908 3645
Size: 1908 1737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1303 1717 2200 2230 2855 3064 3391
Size: 595 708 414 483 30 625 209 327
Cuts at: 3391 3645
Size: 254
Fragments arranged by size:
708 625 595 483 414 327 254 209
30
Bpu10I CC'TnA_GC
Cuts at: 0 1637 2427 2603 3645
Size: 1637 790 176 1042
Fragments arranged by size:
1637 1042 790 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 3052 3220 3405 3645
Size: 378 2674 168 185 240
Fragments arranged by size:
2674 378 240 185 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 428 2467 3645
Size: 428 2039 1178
Fragments arranged by size:
2039 1178 428
BsaAI yAC'GTr
Cuts at: 0 1149 3645
Size: 1149 2496
BsaBI GATnn'nnATC
Cuts at: 0 931 1123 3645
Size: 931 192 2522
Fragments arranged by size:
2522 931 192
BsaHI Gr'CG_yC
Cuts at: 0 320 688 1714 1871 1953 2001 2729 3146
Size: 320 368 1026 157 82 48 728 417
Cuts at: 3146 3176 3353 3645
Size: 30 177 292
Fragments arranged by size:
1026 728 417 368 320 292 177 157
82 48 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1699 1729 3496 3526 3645
Size: 1699 30 1767 30 119
Fragments arranged by size:
1767 1699 119 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 814 1795 1954 1957 2197 2200 2238 2350
Size: 814 981 159 3 240 3 38 112
Cuts at: 2350 2767 2845 2896 2935 2938 2947 3120 3645
Size: 417 78 51 39 3 9 173 525
Fragments arranged by size:
981 814 525 417 240 173 159 112
78 51 39 38 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 218 367 762 1418 1649 1801 2312 2359
Size: 218 149 395 656 231 152 511 47
Cuts at: 2359 3645
Size: 1286
Fragments arranged by size:
1286 656 511 395 231 218 152 149
47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1588 1702 1723 2134 2923 3289 3603
Size: 1033 555 114 21 411 789 366 314
Cuts at: 3603 3645
Size: 42
Fragments arranged by size:
1033 789 555 411 366 314 114 42
21
BsiEI CG_ry'CG
Cuts at: 0 33 1969 3121 3645
Size: 33 1936 1152 524
Fragments arranged by size:
1936 1152 524 33
BsiHKAI G_wGCw'C
Cuts at: 0 741 860 1398 2056 2102 2687 3083 3397
Size: 741 119 538 658 46 585 396 314
Cuts at: 3397 3645
Size: 248
Fragments arranged by size:
741 658 585 538 396 314 248 119
46
BsmI GAATG_Cn'
Cuts at: 0 2355 3413 3645
Size: 2355 1058 232
Fragments arranged by size:
2355 1058 232
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3183 3645
Size: 3183 462
Bsp1286I G_dGCh'C
Cuts at: 0 741 785 860 1080 1291 1398 2056 2102
Size: 741 44 75 220 211 107 658 46
Cuts at: 2102 2403 2687 3083 3130 3397 3645
Size: 301 284 396 47 267 248
Fragments arranged by size:
741 658 396 301 284 267 248 220
211 107 75 47 46 44
BspHI T'CATG_A
Cuts at: 0 452 3645
Size: 452 3193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1094 1409 2307 3645
Size: 1094 315 898 1338
Fragments arranged by size:
1338 1094 898 315
BsrBI CCG'CTC
Cuts at: 0 669 1710 2190 2835 3512 3645
Size: 669 1041 480 645 677 133
Fragments arranged by size:
1041 677 669 645 480 133
BsrDI GCAATG_nn'
Cuts at: 0 983 3254 3645
Size: 983 2271 391
Fragments arranged by size:
2271 983 391
BsrFI r'CCGG_y
Cuts at: 0 1664 1964 2198 2898 3645
Size: 1664 300 234 700 747
Fragments arranged by size:
1664 747 700 300 234
BsrGI T'GTAC_A
Cuts at: 0 509 3503 3645
Size: 509 2994 142
Fragments arranged by size:
2994 509 142
BssHII G'CGCG_C
Cuts at: 0 2912 3645
Size: 2912 733
BssSI C'ACGA_G
Cuts at: 0 1936 3283 3645
Size: 1936 1347 362
Fragments arranged by size:
1936 1347 362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 438 3042 3645
Size: 438 2604 603
Fragments arranged by size:
2604 603 438
BstYI r'GATC_y
Cuts at: 0 344 986 1430 1460 3446 3645
Size: 344 642 444 30 1986 199
Fragments arranged by size:
1986 642 444 344 199 30
BtgI C'CryG_G
Cuts at: 0 66 757 2108 2282 2504 2675 3645
Size: 66 691 1351 174 222 171 970
Fragments arranged by size:
1351 970 691 222 174 171 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 138 1293 2045 2286 3645
Size: 138 1155 752 241 1359
Fragments arranged by size:
1359 1155 752 241 138
BtsI GCAGTG_nn'
Cuts at: 0 860 1045 3591 3645
Size: 860 185 2546 54
Fragments arranged by size:
2546 860 185 54
DrdI GACnn_nn'nnGTC
Cuts at: 0 402 462 2128 3022 3645
Size: 402 60 1666 894 623
Fragments arranged by size:
1666 894 623 402 60
EaeI y'GGCC_r
Cuts at: 0 629 1299 1331 1406 1966 3032 3118 3635
Size: 629 670 32 75 560 1066 86 517
Cuts at: 3635 3645
Size: 10
Fragments arranged by size:
1066 670 629 560 517 86 75 32
10
EagI C'GGCC_G
Cuts at: 0 1966 3118 3645
Size: 1966 1152 527
Fragments arranged by size:
1966 1152 527
EarI CTCTTCn'nnn_
Cuts at: 0 1982 2512 3611 3645
Size: 1982 530 1099 34
Fragments arranged by size:
1982 1099 530 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1993 3645
Size: 1993 1652
EcoICRI GAG'CTC
Cuts at: 0 2054 2685 3081 3395 3645
Size: 2054 631 396 314 250
Fragments arranged by size:
2054 631 396 314 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 544 583 595 1012 1303 1717 1738
Size: 160 384 39 12 417 291 414 21
Cuts at: 1738 2200 2230 2855 3064 3282 3294 3391 3645
Size: 462 30 625 209 218 12 97 254
Fragments arranged by size:
625 462 417 414 384 291 254 218
209 160 97 39 30 21 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 483 3645
Size: 483 3162
EcoO109I rG'GnC_Cy
Cuts at: 0 579 708 1026 1077 2607 3242 3645
Size: 579 129 318 51 1530 635 403
Fragments arranged by size:
1530 635 579 403 318 129 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 3312 3645
Size: 282 3030 333
Fragments arranged by size:
3030 333 282
HaeII r_GCGC'y
Cuts at: 0 896 1637 1793 2004 2265 3645
Size: 896 741 156 211 261 1380
Fragments arranged by size:
1380 896 741 261 211 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 197 229 324 356 1699 1731 1888 1920
Size: 197 32 95 32 1343 32 157 32
Cuts at: 1920 2773 2805 3321 3353 3645
Size: 853 32 516 32 292
Fragments arranged by size:
1343 853 516 292 197 157 95 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3573 3645
Size: 3573 72
HindIII A'AGCT_T
Cuts at: 0 1750 3645
Size: 1750 1895
KasI G'GCGC_C
Cuts at: 0 2000 3645
Size: 2000 1645
KpnI G_GTAC'C
Cuts at: 0 1169 2079 3645
Size: 1169 910 1566
Fragments arranged by size:
1566 1169 910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 1952 3206 3645
Size: 1244 708 1254 439
Fragments arranged by size:
1254 1244 708 439
MscI TGG'CCA
Cuts at: 0 631 1333 1408 3034 3637 3645
Size: 631 702 75 1626 603 8
Fragments arranged by size:
1626 702 631 603 75 8
MslI CAynn'nnrTG
Cuts at: 0 157 241 1264 1339 2107 3220 3418 3645
Size: 157 84 1023 75 768 1113 198 227
Fragments arranged by size:
1113 1023 768 227 198 157 84 75
MspA1I CmG'CkG
Cuts at: 0 68 218 759 1422 1731 1950 2890 3165
Size: 68 150 541 663 309 219 940 275
Cuts at: 3165 3314 3645
Size: 149 331
Fragments arranged by size:
940 663 541 331 309 275 219 150
149 68
NarI GG'CG_CC
Cuts at: 0 2001 3645
Size: 2001 1644
NcoI C'CATG_G
Cuts at: 0 2504 3645
Size: 2504 1141
NspI r_CATG'y
Cuts at: 0 39 882 3645
Size: 39 843 2763
Fragments arranged by size:
2763 843 39
PasI CC'CwG_GG
Cuts at: 0 1922 3645
Size: 1922 1723
PflMI CCAn_nnn'nTGG
Cuts at: 0 704 1323 1506 1656 2319 3645
Size: 704 619 183 150 663 1326
Fragments arranged by size:
1326 704 663 619 183 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3550 3582 3645
Size: 3550 32 63
Fragments arranged by size:
3550 63 32
PpuMI rG'GwC_Cy
Cuts at: 0 579 708 1026 2607 3242 3645
Size: 579 129 318 1581 635 403
Fragments arranged by size:
1581 635 579 403 318 129
PspOMI G'GGCC_C
Cuts at: 0 1076 3645
Size: 1076 2569
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 502 534 943 975 3645
Size: 502 32 409 32 2670
Fragments arranged by size:
2670 502 409 32 32
PstI C_TGCA'G
Cuts at: 0 56 1040 1213 1490 1604 1730 3011 3319
Size: 56 984 173 277 114 126 1281 308
Cuts at: 3319 3645
Size: 326
Fragments arranged by size:
1281 984 326 308 277 173 126 114
56
PvuII CAG'CTG
Cuts at: 0 218 1731 3165 3314 3645
Size: 218 1513 1434 149 331
Fragments arranged by size:
1513 1434 331 218 149
SacI G_AGCT'C
Cuts at: 0 2056 2687 3083 3397 3645
Size: 2056 631 396 314 248
Fragments arranged by size:
2056 631 396 314 248
SacII CC_GC'GG
Cuts at: 0 69 760 3645
Size: 69 691 2885
Fragments arranged by size:
2885 691 69
SalI G'TCGA_C
Cuts at: 0 3571 3645
Size: 3571 74
SanDI GG'GwC_CC
Cuts at: 0 3242 3645
Size: 3242 403
SapI GCTCTTCn'nnn_
Cuts at: 0 1982 2512 3645
Size: 1982 530 1133
Fragments arranged by size:
1982 1133 530
SfcI C'TryA_G
Cuts at: 0 52 1036 1209 1486 1600 1726 2394 3007
Size: 52 984 173 277 114 126 668 613
Cuts at: 3007 3315 3345 3645
Size: 308 30 300
Fragments arranged by size:
984 668 613 308 300 277 173 126
114 52 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1828 3645
Size: 1828 1817
SfoI GGC'GCC
Cuts at: 0 2002 3645
Size: 2002 1643
SmaI CCC'GGG
Cuts at: 0 591 2090 3645
Size: 591 1499 1555
Fragments arranged by size:
1555 1499 591
SmlI C'TyrA_G
Cuts at: 0 7 357 3067 3235 3384 3645
Size: 7 350 2710 168 149 261
Fragments arranged by size:
2710 350 261 168 149 7
SphI G_CATG'C
Cuts at: 0 882 3645
Size: 882 2763
StuI AGG'CCT
Cuts at: 0 1859 3645
Size: 1859 1786
StyI C'CwwG_G
Cuts at: 0 1760 1826 2504 3645
Size: 1760 66 678 1141
Fragments arranged by size:
1760 1141 678 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1432 1663 3645
Size: 1432 231 1982
Fragments arranged by size:
1982 1432 231
TatI w'GTAC_w
Cuts at: 0 509 1229 3503 3645
Size: 509 720 2274 142
Fragments arranged by size:
2274 720 509 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 991 1023 3451 3483 3570 3602 3645
Size: 991 32 2428 32 87 32 43
Fragments arranged by size:
2428 991 87 43 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 61 2326 3246 3645
Size: 61 2265 920 399
Fragments arranged by size:
2265 920 399 61
XmaI C'CCGG_G
Cuts at: 0 589 2088 3645
Size: 589 1499 1557
Fragments arranged by size:
1557 1499 589
ZraI GAC'GTC
Cuts at: 0 321 689 3645
Size: 321 368 2956
Fragments arranged by size:
2956 368 321
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AhdI AloI
AlwNI ApaI ApaLI ApoI AscI AvaI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstXI BstYI BtgI BtgZI
BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PpiI PpuMI PspOMI PsrI PstI PvuII
SacI SacII SalI SanDI SapI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XmaI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AleI AlfI AseI AsiSI
AvrII BaeI BamHI BcgI BfrBI BmtI BsaWI BsiWI
BspEI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII EcoRI EcoRV FseI FspI FspAI HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PshAI PsiI
PspXI PvuI RsrII SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI XcmI XhoI
XmnI