Restriction map for
pF1KB3061
(Linear) (Six-base) MAPSORT of: KIBB3061.seq Check: 1357 from: 1 to: 1665
KIBB3061.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 675 1011 1095 1179 1347 1431 1515
Size: 613 62 336 84 84 168 84 84
Cuts at: 1515 1665
Size: 150
Fragments arranged by size:
613 336 168 150 84 84 84 84
62
AflII C'TTAA_G
Cuts at: 0 522 1665
Size: 522 1143
AflIII A'CryG_T
Cuts at: 0 581 1085 1665
Size: 581 504 580
Fragments arranged by size:
581 580 504
AleI CACnn'nnGTG
Cuts at: 0 734 1406 1665
Size: 734 672 259
Fragments arranged by size:
734 672 259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 1665
Size: 976 689
AlwNI CAG_nnn'CTG
Cuts at: 0 264 1665
Size: 264 1401
ApaLI G'TGCA_C
Cuts at: 0 1147 1367 1425 1471 1651 1665
Size: 1147 220 58 46 180 14
Fragments arranged by size:
1147 220 180 58 46 14
ApoI r'AATT_y
Cuts at: 0 725 1665
Size: 725 940
AseI AT'TA_AT
Cuts at: 0 397 1665
Size: 397 1268
BamHI G'GATC_C
Cuts at: 0 178 893 1061 1229 1565 1665
Size: 178 715 168 168 336 100
Fragments arranged by size:
715 336 178 168 168 100
BanII G_rGCy'C
Cuts at: 0 703 787 1665
Size: 703 84 878
Fragments arranged by size:
878 703 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 1195 1665
Size: 1195 470
BciVI GTATCCnnnnn_n'
Cuts at: 0 652 1665
Size: 652 1013
BclI T'GATC_A
Cuts at: 0 447 1665
Size: 447 1218
BfrBI ATG'CAT
Cuts at: 0 1255 1665
Size: 1255 410
BglII A'GATC_T
Cuts at: 0 789 1665
Size: 789 876
BlpI GC'TnA_GC
Cuts at: 0 797 1665
Size: 797 868
Bme1580I G_kGCm'C
Cuts at: 0 106 1151 1358 1371 1429 1475 1655 1665
Size: 106 1045 207 13 58 46 180 10
Fragments arranged by size:
1045 207 180 106 58 46 13 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 995 1331 1665
Size: 995 336 334
Fragments arranged by size:
995 336 334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 671 1665
Size: 226 445 994
Fragments arranged by size:
994 445 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 862 1665
Size: 402 460 803
Fragments arranged by size:
803 460 402
BsaI GGTCTCn'nnnn_
Cuts at: 0 1382 1665
Size: 1382 283
BsaAI yAC'GTr
Cuts at: 0 1088 1665
Size: 1088 577
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 1665
Size: 181 30 1454
Fragments arranged by size:
1454 181 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 1665
Size: 64 1601
BseYI C'CCAG_C
Cuts at: 0 200 1665
Size: 200 1465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 1110 1194 1665
Size: 690 420 84 471
Fragments arranged by size:
690 471 420 84
BsiEI CG_ry'CG
Cuts at: 0 1119 1665
Size: 1119 546
BsiHKAI G_wGCw'C
Cuts at: 0 74 703 787 1151 1371 1429 1475 1550
Size: 74 629 84 364 220 58 46 75
Cuts at: 1550 1655 1665
Size: 105 10
Fragments arranged by size:
629 364 220 105 84 75 74 58
46 10
Bsp1286I G_dGCh'C
Cuts at: 0 74 106 703 787 1151 1358 1371 1429
Size: 74 32 597 84 364 207 13 58
Cuts at: 1429 1475 1550 1655 1665
Size: 46 75 105 10
Fragments arranged by size:
597 364 207 105 84 75 74 58
46 32 13 10
BsrBI CCG'CTC
Cuts at: 0 1121 1205 1227 1665
Size: 1121 84 22 438
Fragments arranged by size:
1121 438 84 22
BsrDI GCAATG_nn'
Cuts at: 0 1014 1665
Size: 1014 651
BsrFI r'CCGG_y
Cuts at: 0 27 1373 1665
Size: 27 1346 292
Fragments arranged by size:
1346 292 27
BsrGI T'GTAC_A
Cuts at: 0 313 1665
Size: 313 1352
BssSI C'ACGA_G
Cuts at: 0 1222 1665
Size: 1222 443
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1517 1665
Size: 1517 148
BstYI r'GATC_y
Cuts at: 0 178 219 789 893 1061 1229 1565 1665
Size: 178 41 570 104 168 168 336 100
Fragments arranged by size:
570 336 178 168 168 104 100 41
Bsu36I CC'TnA_GG
Cuts at: 0 1477 1665
Size: 1477 188
BtgI C'CryG_G
Cuts at: 0 340 1665
Size: 340 1325
BtsI GCAGTG_nn'
Cuts at: 0 563 678 1042 1182 1665
Size: 563 115 364 140 483
Fragments arranged by size:
563 483 364 140 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 996 1031 1665
Size: 996 35 634
Fragments arranged by size:
996 634 35
DraIII CAC_nnn'GTG
Cuts at: 0 1146 1665
Size: 1146 519
DrdI GACnn_nn'nnGTC
Cuts at: 0 1123 1665
Size: 1123 542
EaeI y'GGCC_r
Cuts at: 0 609 1626 1665
Size: 609 1017 39
Fragments arranged by size:
1017 609 39
EarI CTCTTCn'nnn_
Cuts at: 0 247 1030 1665
Size: 247 783 635
Fragments arranged by size:
783 635 247
EcoICRI GAG'CTC
Cuts at: 0 701 785 1665
Size: 701 84 880
Fragments arranged by size:
880 701 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 613 671 675 1011 1095 1179 1347
Size: 226 387 58 4 336 84 84 168
Cuts at: 1347 1431 1515 1665
Size: 84 84 150
Fragments arranged by size:
387 336 226 168 150 84 84 84
84 58 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 438 1665
Size: 79 359 1227
Fragments arranged by size:
1227 359 79
EcoO109I rG'GnC_Cy
Cuts at: 0 34 117 228 261 575 1481 1665
Size: 34 83 111 33 314 906 184
Fragments arranged by size:
906 314 184 111 83 34 33
EcoRI G'AATT_C
Cuts at: 0 725 1665
Size: 725 940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 69 492 1665
Size: 69 423 1173
Fragments arranged by size:
1173 423 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 316 348 432 464 774 806
Size: 181 32 103 32 84 32 310 32
Cuts at: 806 1665
Size: 859
Fragments arranged by size:
859 310 181 103 84 32 32 32
32
HincII GTy'rAC
Cuts at: 0 501 1665
Size: 501 1164
HindIII A'AGCT_T
Cuts at: 0 474 1665
Size: 474 1191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1665
Size: 829 836
MscI TGG'CCA
Cuts at: 0 1628 1665
Size: 1628 37
MslI CAynn'nnrTG
Cuts at: 0 345 734 918 1213 1406 1506 1665
Size: 345 389 184 295 193 100 159
Fragments arranged by size:
389 345 295 193 184 159 100
MspA1I CmG'CkG
Cuts at: 0 204 891 1563 1665
Size: 204 687 672 102
Fragments arranged by size:
687 672 204 102
NaeI GCC'GGC
Cuts at: 0 29 1665
Size: 29 1636
NcoI C'CATG_G
Cuts at: 0 340 1665
Size: 340 1325
NdeI CA'TA_TG
Cuts at: 0 915 1503 1665
Size: 915 588 162
Fragments arranged by size:
915 588 162
NgoMIV G'CCGG_C
Cuts at: 0 27 1665
Size: 27 1638
NsiI A_TGCA'T
Cuts at: 0 1257 1665
Size: 1257 408
NspI r_CATG'y
Cuts at: 0 537 585 1259 1665
Size: 537 48 674 406
Fragments arranged by size:
674 537 406 48
PasI CC'CwG_GG
Cuts at: 0 224 1665
Size: 224 1441
PciI A'CATG_T
Cuts at: 0 581 1665
Size: 581 1084
PfoI T'CCnGG_A
Cuts at: 0 174 230 1665
Size: 174 56 1435
Fragments arranged by size:
1435 174 56
PmlI CAC'GTG
Cuts at: 0 1088 1665
Size: 1088 577
PpuMI rG'GwC_Cy
Cuts at: 0 34 228 1481 1665
Size: 34 194 1253 184
Fragments arranged by size:
1253 194 184 34
PshAI GACnn'nnGTC
Cuts at: 0 383 1665
Size: 383 1282
PstI C_TGCA'G
Cuts at: 0 1052 1665
Size: 1052 613
PvuII CAG'CTG
Cuts at: 0 204 1665
Size: 204 1461
SacI G_AGCT'C
Cuts at: 0 703 787 1665
Size: 703 84 878
Fragments arranged by size:
878 703 84
SapI GCTCTTCn'nnn_
Cuts at: 0 247 1665
Size: 247 1418
ScaI AGT'ACT
Cuts at: 0 1540 1665
Size: 1540 125
SfcI C'TryA_G
Cuts at: 0 123 305 1048 1665
Size: 123 182 743 617
Fragments arranged by size:
743 617 182 123
SmlI C'TyrA_G
Cuts at: 0 381 522 841 1665
Size: 381 141 319 824
Fragments arranged by size:
824 381 319 141
SphI G_CATG'C
Cuts at: 0 537 1259 1665
Size: 537 722 406
Fragments arranged by size:
722 537 406
StuI AGG'CCT
Cuts at: 0 688 1108 1276 1528 1665
Size: 688 420 168 252 137
Fragments arranged by size:
688 420 252 168 137
StyI C'CwwG_G
Cuts at: 0 340 356 434 1665
Size: 340 16 78 1231
Fragments arranged by size:
1231 340 78 16
TatI w'GTAC_w
Cuts at: 0 313 1538 1665
Size: 313 1225 127
Fragments arranged by size:
1225 313 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 1283 1315 1665
Size: 85 32 1166 32 350
Fragments arranged by size:
1166 350 85 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 688 856 940 1024 1108 1192 1276 1528
Size: 688 168 84 84 84 84 84 252
Cuts at: 1528 1665
Size: 137
Fragments arranged by size:
688 252 168 137 84 84 84 84
84
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI AlwNI ApaLI ApoI
AseI BamHI BanII BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI
CspCI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PfoI PmlI PpuMI PshAI PstI PvuII
SacI SapI ScaI SfcI SmlI SphI StuI StyI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI ApaI AscI AsiSI AvaI AvrII BaeI BanI
BbeI BbvCI BcgI BglI BmgBI BmtI BplI Bpu10I
BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BspMI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI
DraI EagI EciI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NarI NheI NotI
NruI PacI PflMI PmeI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI