Restriction map for
pF1KB3063
(Linear) (Six-base) MAPSORT of: KIBB3063.seq Check: 2649 from: 1 to: 2145
KIBB3063.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 929 2145
Size: 929 1216
AclI AA'CG_TT
Cuts at: 0 301 2145
Size: 301 1844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 551 952 1570 2145
Size: 226 325 401 618 575
Fragments arranged by size:
618 575 401 325 226
AfeI AGC'GCT
Cuts at: 0 1070 1238 2145
Size: 1070 168 907
Fragments arranged by size:
1070 907 168
AflIII A'CryG_T
Cuts at: 0 85 1661 2145
Size: 85 1576 484
Fragments arranged by size:
1576 484 85
AleI CACnn'nnGTG
Cuts at: 0 2017 2145
Size: 2017 128
AlwNI CAG_nnn'CTG
Cuts at: 0 631 2145
Size: 631 1514
ApaLI G'TGCA_C
Cuts at: 0 854 2145
Size: 854 1291
ApoI r'AATT_y
Cuts at: 0 130 258 818 1909 2145
Size: 130 128 560 1091 236
Fragments arranged by size:
1091 560 236 130 128
AseI AT'TA_AT
Cuts at: 0 52 323 2145
Size: 52 271 1822
Fragments arranged by size:
1822 271 52
AvaI C'yCGr_G
Cuts at: 0 263 1072 1949 2145
Size: 263 809 877 196
Fragments arranged by size:
877 809 263 196
BanI G'GyrC_C
Cuts at: 0 697 1308 2145
Size: 697 611 837
Fragments arranged by size:
837 697 611
BanII G_rGCy'C
Cuts at: 0 689 1602 1743 2145
Size: 689 913 141 402
Fragments arranged by size:
913 689 402 141
BbeI G_GCGC'C
Cuts at: 0 1312 2145
Size: 1312 833
BbsI GAAGACnn'nnnn_
Cuts at: 0 1550 1581 2145
Size: 1550 31 564
Fragments arranged by size:
1550 564 31
BbvCI CC'TCA_GC
Cuts at: 0 664 2145
Size: 664 1481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 857 1948 1982 2145
Size: 823 34 1091 34 163
Fragments arranged by size:
1091 823 163 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2075 2145
Size: 2075 70
Bme1580I G_kGCm'C
Cuts at: 0 536 700 858 2145
Size: 536 164 158 1287
Fragments arranged by size:
1287 536 164 158
BmgBI CAC'GTC
Cuts at: 0 908 1662 1962 2145
Size: 908 754 300 183
Fragments arranged by size:
908 754 300 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 16 1294 2145
Size: 16 1278 851
Fragments arranged by size:
1278 851 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1433 1555 2080 2094 2109 2124 2145
Size: 781 652 122 525 14 15 15 21
Fragments arranged by size:
781 652 525 122 21 15 15 14
Bpu10I CC'TnA_GC
Cuts at: 0 664 683 1030 1123 2145
Size: 664 19 347 93 1022
Fragments arranged by size:
1022 664 347 93 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 2145
Size: 622 1523
BsaI GGTCTCn'nnnn_
Cuts at: 0 796 1215 1558 1986 2145
Size: 796 419 343 428 159
Fragments arranged by size:
796 428 419 343 159
BsaAI yAC'GTr
Cuts at: 0 1026 2145
Size: 1026 1119
BsaHI Gr'CG_yC
Cuts at: 0 1309 2145
Size: 1309 836
BsaWI w'CCGG_w
Cuts at: 0 1517 2145
Size: 1517 628
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1087 1117 1212 1242 1459 1489 1841 1871
Size: 1087 30 95 30 217 30 352 30
Cuts at: 1871 2113 2143 2145
Size: 242 30 2
Fragments arranged by size:
1087 352 242 217 95 30 30 30
30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 947 1543 2097 2145
Size: 947 596 554 48
Fragments arranged by size:
947 596 554 48
BseYI C'CCAG_C
Cuts at: 0 673 1035 1800 2084 2145
Size: 673 362 765 284 61
Fragments arranged by size:
765 673 362 284 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1795 1879 2145
Size: 25 1770 84 266
Fragments arranged by size:
1770 266 84 25
BsiHKAI G_wGCw'C
Cuts at: 0 858 1031 1331 2145
Size: 858 173 300 814
Fragments arranged by size:
858 814 300 173
Bsp1286I G_dGCh'C
Cuts at: 0 536 689 700 858 1031 1331 1602 1743
Size: 536 153 11 158 173 300 271 141
Cuts at: 1743 2145
Size: 402
Fragments arranged by size:
536 402 300 271 173 158 153 141
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1186 1968 2145
Size: 1186 782 177
Fragments arranged by size:
1186 782 177
BsrDI GCAATG_nn'
Cuts at: 0 330 449 2145
Size: 330 119 1696
Fragments arranged by size:
1696 330 119
BsrGI T'GTAC_A
Cuts at: 0 32 2145
Size: 32 2113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1059 1411 2145
Size: 1059 352 734
Fragments arranged by size:
1059 734 352
Bsu36I CC'TnA_GG
Cuts at: 0 959 2145
Size: 959 1186
BtgI C'CryG_G
Cuts at: 0 608 689 2145
Size: 608 81 1456
Fragments arranged by size:
1456 608 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1143 2145
Size: 1143 1002
BtsI GCAGTG_nn'
Cuts at: 0 120 1909 2145
Size: 120 1789 236
Fragments arranged by size:
1789 236 120
EaeI y'GGCC_r
Cuts at: 0 611 1882 2145
Size: 611 1271 263
Fragments arranged by size:
1271 611 263
EarI CTCTTCn'nnn_
Cuts at: 0 570 1130 1989 2145
Size: 570 560 859 156
Fragments arranged by size:
859 570 560 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 536 2145
Size: 536 1609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 551 781 952 1433 1555 1570 2080
Size: 226 325 230 171 481 122 15 510
Cuts at: 2080 2094 2109 2124 2145
Size: 14 15 15 21
Fragments arranged by size:
510 481 325 230 226 171 122 21
15 15 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 69 640 2145
Size: 69 571 1505
Fragments arranged by size:
1505 571 69
EcoO109I rG'GnC_Cy
Cuts at: 0 963 1408 2145
Size: 963 445 737
Fragments arranged by size:
963 737 445
EcoRI G'AATT_C
Cuts at: 0 130 258 2145
Size: 130 128 1887
Fragments arranged by size:
1887 130 128
HaeII r_GCGC'y
Cuts at: 0 174 1072 1240 1312 2145
Size: 174 898 168 72 833
Fragments arranged by size:
898 833 174 168 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 879 911 942 974 1085 1117
Size: 388 32 459 32 31 32 111 32
Cuts at: 1117 1621 1653 2145
Size: 504 32 492
Fragments arranged by size:
504 492 459 388 111 32 32 32
32 32 31
KasI G'GCGC_C
Cuts at: 0 1308 2145
Size: 1308 837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 2145
Size: 520 1625
MslI CAynn'nnrTG
Cuts at: 0 1255 2017 2145
Size: 1255 762 128
Fragments arranged by size:
1255 762 128
MspA1I CmG'CkG
Cuts at: 0 759 1800 2075 2088 2145
Size: 759 1041 275 13 57
Fragments arranged by size:
1041 759 275 57 13
NarI GG'CG_CC
Cuts at: 0 1309 2145
Size: 1309 836
NcoI C'CATG_G
Cuts at: 0 608 2145
Size: 608 1537
NspI r_CATG'y
Cuts at: 0 89 1209 1476 1923 2145
Size: 89 1120 267 447 222
Fragments arranged by size:
1120 447 267 222 89
PasI CC'CwG_GG
Cuts at: 0 1313 1734 2145
Size: 1313 421 411
Fragments arranged by size:
1313 421 411
PciI A'CATG_T
Cuts at: 0 85 2145
Size: 85 2060
PflMI CCAn_nnn'nTGG
Cuts at: 0 1492 2145
Size: 1492 653
PfoI T'CCnGG_A
Cuts at: 0 797 1410 2145
Size: 797 613 735
Fragments arranged by size:
797 735 613
PpuMI rG'GwC_Cy
Cuts at: 0 963 1408 2145
Size: 963 445 737
Fragments arranged by size:
963 737 445
PsiI TTA'TAA
Cuts at: 0 293 2145
Size: 293 1852
PstI C_TGCA'G
Cuts at: 0 764 1527 1904 1979 2145
Size: 764 763 377 75 166
Fragments arranged by size:
764 763 377 166 75
PvuII CAG'CTG
Cuts at: 0 1800 2088 2145
Size: 1800 288 57
Fragments arranged by size:
1800 288 57
SbfI CC_TGCA'GG
Cuts at: 0 1979 2145
Size: 1979 166
SexAI A'CCwGG_T
Cuts at: 0 1224 1876 2145
Size: 1224 652 269
Fragments arranged by size:
1224 652 269
SfcI C'TryA_G
Cuts at: 0 760 1523 1812 1900 1975 2145
Size: 760 763 289 88 75 170
Fragments arranged by size:
763 760 289 170 88 75
SfoI GGC'GCC
Cuts at: 0 1310 2145
Size: 1310 835
SmaI CCC'GGG
Cuts at: 0 1951 2145
Size: 1951 194
SmlI C'TyrA_G
Cuts at: 0 637 2145
Size: 637 1508
SphI G_CATG'C
Cuts at: 0 1923 2145
Size: 1923 222
SspI AAT'ATT
Cuts at: 0 870 2145
Size: 870 1275
StuI AGG'CCT
Cuts at: 0 1418 2145
Size: 1418 727
StyI C'CwwG_G
Cuts at: 0 608 2145
Size: 608 1537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 210 1734 2145
Size: 210 1524 411
Fragments arranged by size:
1524 411 210
TatI w'GTAC_w
Cuts at: 0 32 424 2006 2145
Size: 32 392 1582 139
Fragments arranged by size:
1582 392 139 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1212 1244 1459 1491 1841 1873 2113 2145
Size: 1212 32 215 32 350 32 240 32
Fragments arranged by size:
1212 350 240 215 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1789 2145
Size: 1789 356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2055 2145
Size: 2055 90
XmaI C'CCGG_G
Cuts at: 0 1949 2145
Size: 1949 196
XmnI GAAnn'nnTTC
Cuts at: 0 130 2145
Size: 130 2015
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AleI AlwNI ApaLI
ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstXI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I KasI MmeI MslI MspA1I NarI
NcoI NspI PasI PciI PflMI PfoI PpuMI PsiI
PstI PvuII SbfI SexAI SfcI SfoI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AlfI AloI
ApaI AscI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BglII BlpI BmtI BplI BsaBI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI
ZraI