(Linear) (Six-base) MAPSORT of: KIBB3063.seq Check: 2649 from: 1 to: 2145 KIBB3063.orf With 182 enzymes: SgfI * July 18, 2007 13:36 .. AccI GT'mk_AC Cuts at: 0 929 2145 Size: 929 1216 AclI AA'CG_TT Cuts at: 0 301 2145 Size: 301 1844 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 551 952 1570 2145 Size: 226 325 401 618 575 Fragments arranged by size: 618 575 401 325 226 AfeI AGC'GCT Cuts at: 0 1070 1238 2145 Size: 1070 168 907 Fragments arranged by size: 1070 907 168 AflIII A'CryG_T Cuts at: 0 85 1661 2145 Size: 85 1576 484 Fragments arranged by size: 1576 484 85 AleI CACnn'nnGTG Cuts at: 0 2017 2145 Size: 2017 128 AlwNI CAG_nnn'CTG Cuts at: 0 631 2145 Size: 631 1514 ApaLI G'TGCA_C Cuts at: 0 854 2145 Size: 854 1291 ApoI r'AATT_y Cuts at: 0 130 258 818 1909 2145 Size: 130 128 560 1091 236 Fragments arranged by size: 1091 560 236 130 128 AseI AT'TA_AT Cuts at: 0 52 323 2145 Size: 52 271 1822 Fragments arranged by size: 1822 271 52 AvaI C'yCGr_G Cuts at: 0 263 1072 1949 2145 Size: 263 809 877 196 Fragments arranged by size: 877 809 263 196 BanI G'GyrC_C Cuts at: 0 697 1308 2145 Size: 697 611 837 Fragments arranged by size: 837 697 611 BanII G_rGCy'C Cuts at: 0 689 1602 1743 2145 Size: 689 913 141 402 Fragments arranged by size: 913 689 402 141 BbeI G_GCGC'C Cuts at: 0 1312 2145 Size: 1312 833 BbsI GAAGACnn'nnnn_ Cuts at: 0 1550 1581 2145 Size: 1550 31 564 Fragments arranged by size: 1550 564 31 BbvCI CC'TCA_GC Cuts at: 0 664 2145 Size: 664 1481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 823 857 1948 1982 2145 Size: 823 34 1091 34 163 Fragments arranged by size: 1091 823 163 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 2075 2145 Size: 2075 70 Bme1580I G_kGCm'C Cuts at: 0 536 700 858 2145 Size: 536 164 158 1287 Fragments arranged by size: 1287 536 164 158 BmgBI CAC'GTC Cuts at: 0 908 1662 1962 2145 Size: 908 754 300 183 Fragments arranged by size: 908 754 300 183 BmrI ACTGGGnnnn_n' Cuts at: 0 16 1294 2145 Size: 16 1278 851 Fragments arranged by size: 1278 851 16 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 781 1433 1555 2080 2094 2109 2124 2145 Size: 781 652 122 525 14 15 15 21 Fragments arranged by size: 781 652 525 122 21 15 15 14 Bpu10I CC'TnA_GC Cuts at: 0 664 683 1030 1123 2145 Size: 664 19 347 93 1022 Fragments arranged by size: 1022 664 347 93 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 2145 Size: 622 1523 BsaI GGTCTCn'nnnn_ Cuts at: 0 796 1215 1558 1986 2145 Size: 796 419 343 428 159 Fragments arranged by size: 796 428 419 343 159 BsaAI yAC'GTr Cuts at: 0 1026 2145 Size: 1026 1119 BsaHI Gr'CG_yC Cuts at: 0 1309 2145 Size: 1309 836 BsaWI w'CCGG_w Cuts at: 0 1517 2145 Size: 1517 628 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1087 1117 1212 1242 1459 1489 1841 1871 Size: 1087 30 95 30 217 30 352 30 Cuts at: 1871 2113 2143 2145 Size: 242 30 2 Fragments arranged by size: 1087 352 242 217 95 30 30 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 947 1543 2097 2145 Size: 947 596 554 48 Fragments arranged by size: 947 596 554 48 BseYI C'CCAG_C Cuts at: 0 673 1035 1800 2084 2145 Size: 673 362 765 284 61 Fragments arranged by size: 765 673 362 284 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 1795 1879 2145 Size: 25 1770 84 266 Fragments arranged by size: 1770 266 84 25 BsiHKAI G_wGCw'C Cuts at: 0 858 1031 1331 2145 Size: 858 173 300 814 Fragments arranged by size: 858 814 300 173 Bsp1286I G_dGCh'C Cuts at: 0 536 689 700 858 1031 1331 1602 1743 Size: 536 153 11 158 173 300 271 141 Cuts at: 1743 2145 Size: 402 Fragments arranged by size: 536 402 300 271 173 158 153 141 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1186 1968 2145 Size: 1186 782 177 Fragments arranged by size: 1186 782 177 BsrDI GCAATG_nn' Cuts at: 0 330 449 2145 Size: 330 119 1696 Fragments arranged by size: 1696 330 119 BsrGI T'GTAC_A Cuts at: 0 32 2145 Size: 32 2113 BstXI CCAn_nnnn'nTGG Cuts at: 0 1059 1411 2145 Size: 1059 352 734 Fragments arranged by size: 1059 734 352 Bsu36I CC'TnA_GG Cuts at: 0 959 2145 Size: 959 1186 BtgI C'CryG_G Cuts at: 0 608 689 2145 Size: 608 81 1456 Fragments arranged by size: 1456 608 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1143 2145 Size: 1143 1002 BtsI GCAGTG_nn' Cuts at: 0 120 1909 2145 Size: 120 1789 236 Fragments arranged by size: 1789 236 120 EaeI y'GGCC_r Cuts at: 0 611 1882 2145 Size: 611 1271 263 Fragments arranged by size: 1271 611 263 EarI CTCTTCn'nnn_ Cuts at: 0 570 1130 1989 2145 Size: 570 560 859 156 Fragments arranged by size: 859 570 560 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 536 2145 Size: 536 1609 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 551 781 952 1433 1555 1570 2080 Size: 226 325 230 171 481 122 15 510 Cuts at: 2080 2094 2109 2124 2145 Size: 14 15 15 21 Fragments arranged by size: 510 481 325 230 226 171 122 21 15 15 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 69 640 2145 Size: 69 571 1505 Fragments arranged by size: 1505 571 69 EcoO109I rG'GnC_Cy Cuts at: 0 963 1408 2145 Size: 963 445 737 Fragments arranged by size: 963 737 445 EcoRI G'AATT_C Cuts at: 0 130 258 2145 Size: 130 128 1887 Fragments arranged by size: 1887 130 128 HaeII r_GCGC'y Cuts at: 0 174 1072 1240 1312 2145 Size: 174 898 168 72 833 Fragments arranged by size: 898 833 174 168 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 388 420 879 911 942 974 1085 1117 Size: 388 32 459 32 31 32 111 32 Cuts at: 1117 1621 1653 2145 Size: 504 32 492 Fragments arranged by size: 504 492 459 388 111 32 32 32 32 32 31 KasI G'GCGC_C Cuts at: 0 1308 2145 Size: 1308 837 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 520 2145 Size: 520 1625 MslI CAynn'nnrTG Cuts at: 0 1255 2017 2145 Size: 1255 762 128 Fragments arranged by size: 1255 762 128 MspA1I CmG'CkG Cuts at: 0 759 1800 2075 2088 2145 Size: 759 1041 275 13 57 Fragments arranged by size: 1041 759 275 57 13 NarI GG'CG_CC Cuts at: 0 1309 2145 Size: 1309 836 NcoI C'CATG_G Cuts at: 0 608 2145 Size: 608 1537 NspI r_CATG'y Cuts at: 0 89 1209 1476 1923 2145 Size: 89 1120 267 447 222 Fragments arranged by size: 1120 447 267 222 89 PasI CC'CwG_GG Cuts at: 0 1313 1734 2145 Size: 1313 421 411 Fragments arranged by size: 1313 421 411 PciI A'CATG_T Cuts at: 0 85 2145 Size: 85 2060 PflMI CCAn_nnn'nTGG Cuts at: 0 1492 2145 Size: 1492 653 PfoI T'CCnGG_A Cuts at: 0 797 1410 2145 Size: 797 613 735 Fragments arranged by size: 797 735 613 PpuMI rG'GwC_Cy Cuts at: 0 963 1408 2145 Size: 963 445 737 Fragments arranged by size: 963 737 445 PsiI TTA'TAA Cuts at: 0 293 2145 Size: 293 1852 PstI C_TGCA'G Cuts at: 0 764 1527 1904 1979 2145 Size: 764 763 377 75 166 Fragments arranged by size: 764 763 377 166 75 PvuII CAG'CTG Cuts at: 0 1800 2088 2145 Size: 1800 288 57 Fragments arranged by size: 1800 288 57 SbfI CC_TGCA'GG Cuts at: 0 1979 2145 Size: 1979 166 SexAI A'CCwGG_T Cuts at: 0 1224 1876 2145 Size: 1224 652 269 Fragments arranged by size: 1224 652 269 SfcI C'TryA_G Cuts at: 0 760 1523 1812 1900 1975 2145 Size: 760 763 289 88 75 170 Fragments arranged by size: 763 760 289 170 88 75 SfoI GGC'GCC Cuts at: 0 1310 2145 Size: 1310 835 SmaI CCC'GGG Cuts at: 0 1951 2145 Size: 1951 194 SmlI C'TyrA_G Cuts at: 0 637 2145 Size: 637 1508 SphI G_CATG'C Cuts at: 0 1923 2145 Size: 1923 222 SspI AAT'ATT Cuts at: 0 870 2145 Size: 870 1275 StuI AGG'CCT Cuts at: 0 1418 2145 Size: 1418 727 StyI C'CwwG_G Cuts at: 0 608 2145 Size: 608 1537 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 210 1734 2145 Size: 210 1524 411 Fragments arranged by size: 1524 411 210 TatI w'GTAC_w Cuts at: 0 32 424 2006 2145 Size: 32 392 1582 139 Fragments arranged by size: 1582 392 139 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1212 1244 1459 1491 1841 1873 2113 2145 Size: 1212 32 215 32 350 32 240 32 Fragments arranged by size: 1212 350 240 215 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1789 2145 Size: 1789 356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2055 2145 Size: 2055 90 XmaI C'CCGG_G Cuts at: 0 1949 2145 Size: 1949 196 XmnI GAAnn'nnTTC Cuts at: 0 130 2145 Size: 130 2015 Enzymes that do cut: AccI AclI AcuI AfeI AflIII AleI AlwNI ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstXI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PpuMI PsiI PstI PvuII SbfI SexAI SfcI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AflII AgeI AhdI AlfI AloI ApaI AscI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI BmtI BplI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI ZraI