Restriction map for
pF1KB3065
(Linear) (Six-base) MAPSORT of: KIBB3065.seq Check: 1341 from: 1 to: 1416
KIBB3065.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 512 1416
Size: 512 904
AflIII A'CryG_T
Cuts at: 0 533 560 881 959 1397 1416
Size: 533 27 321 78 438 19
Fragments arranged by size:
533 438 321 78 27 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1359 1416
Size: 1359 57
AlwNI CAG_nnn'CTG
Cuts at: 0 348 1263 1416
Size: 348 915 153
Fragments arranged by size:
915 348 153
ApaI G_GGCC'C
Cuts at: 0 1072 1416
Size: 1072 344
ApoI r'AATT_y
Cuts at: 0 139 238 1416
Size: 139 99 1178
Fragments arranged by size:
1178 139 99
BanI G'GyrC_C
Cuts at: 0 379 934 1416
Size: 379 555 482
Fragments arranged by size:
555 482 379
BanII G_rGCy'C
Cuts at: 0 73 1072 1416
Size: 73 999 344
Fragments arranged by size:
999 344 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 1416
Size: 98 1318
BclI T'GATC_A
Cuts at: 0 1055 1416
Size: 1055 361
BglI GCCn_nnn'nGGC
Cuts at: 0 703 1416
Size: 703 713
Bme1580I G_kGCm'C
Cuts at: 0 382 1072 1416
Size: 382 690 344
Fragments arranged by size:
690 382 344
BmgBI CAC'GTC
Cuts at: 0 962 1400 1416
Size: 962 438 16
Fragments arranged by size:
962 438 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 586 1416
Size: 586 830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1416
Size: 415 1001
BsaAI yAC'GTr
Cuts at: 0 66 534 1416
Size: 66 468 882
Fragments arranged by size:
882 468 66
BsaWI w'CCGG_w
Cuts at: 0 1223 1416
Size: 1223 193
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 1212 1416
Size: 292 920 204
Fragments arranged by size:
920 292 204
BseYI C'CCAG_C
Cuts at: 0 203 213 337 375 1064 1416
Size: 203 10 124 38 689 352
Fragments arranged by size:
689 352 203 124 38 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1279 1416
Size: 1279 137
BsiHKAI G_wGCw'C
Cuts at: 0 1357 1416
Size: 1357 59
BsmI GAATG_Cn'
Cuts at: 0 1138 1416
Size: 1138 278
Bsp1286I G_dGCh'C
Cuts at: 0 73 382 1072 1357 1416
Size: 73 309 690 285 59
Fragments arranged by size:
690 309 285 73 59
BspEI T'CCGG_A
Cuts at: 0 1223 1416
Size: 1223 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1217 1416
Size: 1217 199
BsrDI GCAATG_nn'
Cuts at: 0 353 1416
Size: 353 1063
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1312 1416
Size: 1312 104
BstEII G'GTnAC_C
Cuts at: 0 843 984 1374 1416
Size: 843 141 390 42
Fragments arranged by size:
843 390 141 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 1032 1416
Size: 699 333 384
Fragments arranged by size:
699 384 333
BtgI C'CryG_G
Cuts at: 0 608 698 1416
Size: 608 90 718
Fragments arranged by size:
718 608 90
BtsI GCAGTG_nn'
Cuts at: 0 599 1250 1416
Size: 599 651 166
Fragments arranged by size:
651 599 166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 561 596 1416
Size: 561 35 820
Fragments arranged by size:
820 561 35
EaeI y'GGCC_r
Cuts at: 0 350 991 1416
Size: 350 641 425
Fragments arranged by size:
641 425 350
EarI CTCTTCn'nnn_
Cuts at: 0 937 1416
Size: 937 479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1416
Size: 415 1001
EcoNI CCTnn'n_nnAGG
Cuts at: 0 672 1416
Size: 672 744
EcoO109I rG'GnC_Cy
Cuts at: 0 659 1068 1069 1206 1416
Size: 659 409 1 137 210
Fragments arranged by size:
659 409 210 137 1
FspI TGC'GCA
Cuts at: 0 1256 1416
Size: 1256 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1416
Size: 97 32 1287
Fragments arranged by size:
1287 97 32
MscI TGG'CCA
Cuts at: 0 352 1416
Size: 352 1064
MslI CAynn'nnrTG
Cuts at: 0 812 1384 1416
Size: 812 572 32
Fragments arranged by size:
812 572 32
MspA1I CmG'CkG
Cuts at: 0 348 443 1416
Size: 348 95 973
Fragments arranged by size:
973 348 95
NspI r_CATG'y
Cuts at: 0 885 1233 1416
Size: 885 348 183
Fragments arranged by size:
885 348 183
PciI A'CATG_T
Cuts at: 0 881 1416
Size: 881 535
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 1416
Size: 348 1068
PfoI T'CCnGG_A
Cuts at: 0 392 1416
Size: 392 1024
PmlI CAC'GTG
Cuts at: 0 534 1416
Size: 534 882
PpuMI rG'GwC_Cy
Cuts at: 0 1206 1416
Size: 1206 210
PshAI GACnn'nnGTC
Cuts at: 0 1033 1416
Size: 1033 383
PspOMI G'GGCC_C
Cuts at: 0 1068 1416
Size: 1068 348
PstI C_TGCA'G
Cuts at: 0 448 1147 1416
Size: 448 699 269
Fragments arranged by size:
699 448 269
PvuII CAG'CTG
Cuts at: 0 348 1416
Size: 348 1068
SapI GCTCTTCn'nnn_
Cuts at: 0 937 1416
Size: 937 479
SexAI A'CCwGG_T
Cuts at: 0 221 1416
Size: 221 1195
SfcI C'TryA_G
Cuts at: 0 444 513 1143 1416
Size: 444 69 630 273
Fragments arranged by size:
630 444 273 69
StuI AGG'CCT
Cuts at: 0 450 1416
Size: 450 966
StyI C'CwwG_G
Cuts at: 0 692 1370 1416
Size: 692 678 46
Fragments arranged by size:
692 678 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 568 952 1416
Size: 568 384 464
Fragments arranged by size:
568 464 384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 301 333 1416
Size: 125 32 144 32 1083
Fragments arranged by size:
1083 144 125 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 699 1416
Size: 345 354 717
Fragments arranged by size:
717 354 345
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaI ApoI BanI BanII
BbsI BclI BglI Bme1580I BmgBI BmrI BpmI BsaAI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BstAPI BstEII BstXI BtgI BtsI CspCI
EaeI EarI Eco57MI EcoNI EcoO109I FspI Hin4I MscI
MslI MspA1I NspI PciI PflMI PfoI PmlI PpuMI
PshAI PspOMI PstI PvuII SapI SexAI SfcI StuI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI