Restriction map for
pF1KB3072
(Linear) (Six-base) MAPSORT of: KIBB3072.seq Check: 5502 from: 1 to: 1866
KIBB3072.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1507 1738 1866
Size: 1507 231 128
Fragments arranged by size:
1507 231 128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 958 1866
Size: 628 330 908
Fragments arranged by size:
908 628 330
AgeI A'CCGG_T
Cuts at: 0 1796 1866
Size: 1796 70
AhdI GACnn_n'nnGTC
Cuts at: 0 996 1559 1866
Size: 996 563 307
Fragments arranged by size:
996 563 307
AleI CACnn'nnGTG
Cuts at: 0 55 1866
Size: 55 1811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 970 1537 1866
Size: 970 567 329
Fragments arranged by size:
970 567 329
AlwNI CAG_nnn'CTG
Cuts at: 0 124 365 456 498 612 795 876 1080
Size: 124 241 91 42 114 183 81 204
Cuts at: 1080 1452 1671 1866
Size: 372 219 195
Fragments arranged by size:
372 241 219 204 195 183 124 114
91 81 42
ApaLI G'TGCA_C
Cuts at: 0 1189 1866
Size: 1189 677
ApoI r'AATT_y
Cuts at: 0 201 318 1866
Size: 201 117 1548
Fragments arranged by size:
1548 201 117
AvaI C'yCGr_G
Cuts at: 0 155 904 1008 1246 1640 1866
Size: 155 749 104 238 394 226
Fragments arranged by size:
749 394 238 226 155 104
BanI G'GyrC_C
Cuts at: 0 49 333 1866
Size: 49 284 1533
Fragments arranged by size:
1533 284 49
BanII G_rGCy'C
Cuts at: 0 16 118 214 449 866 1621 1866
Size: 16 102 96 235 417 755 245
Fragments arranged by size:
755 417 245 235 102 96 16
BbvCI CC'TCA_GC
Cuts at: 0 137 1866
Size: 137 1729
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1485 1519 1866
Size: 1485 34 347
Fragments arranged by size:
1485 347 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 791 1472 1866
Size: 791 681 394
Fragments arranged by size:
791 681 394
BglI GCCn_nnn'nGGC
Cuts at: 0 31 977 1866
Size: 31 946 889
Fragments arranged by size:
946 889 31
BlpI GC'TnA_GC
Cuts at: 0 117 1213 1438 1866
Size: 117 1096 225 428
Fragments arranged by size:
1096 428 225 117
Bme1580I G_kGCm'C
Cuts at: 0 959 1193 1759 1866
Size: 959 234 566 107
Fragments arranged by size:
959 566 234 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 808 1866
Size: 808 1058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 300 1034 1866
Size: 300 734 832
Fragments arranged by size:
832 734 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 173 215 433 906 1195 1219 1338
Size: 164 9 42 218 473 289 24 119
Cuts at: 1338 1474 1866
Size: 136 392
Fragments arranged by size:
473 392 289 218 164 136 119 42
24 9
Bpu10I CC'TnA_GC
Cuts at: 0 137 1866
Size: 137 1729
BsaI GGTCTCn'nnnn_
Cuts at: 0 140 255 1866
Size: 140 115 1611
Fragments arranged by size:
1611 140 115
BsaAI yAC'GTr
Cuts at: 0 1584 1866
Size: 1584 282
BsaHI Gr'CG_yC
Cuts at: 0 1560 1866
Size: 1560 306
BsaWI w'CCGG_w
Cuts at: 0 1796 1866
Size: 1796 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 94 1611 1759 1866
Size: 24 70 1517 148 107
Fragments arranged by size:
1517 148 107 70 24
BseYI C'CCAG_C
Cuts at: 0 26 150 687 734 869 967 1158 1866
Size: 26 124 537 47 135 98 191 708
Fragments arranged by size:
708 537 191 135 124 98 47 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 781 1066 1630 1866
Size: 48 733 285 564 236
Fragments arranged by size:
733 564 285 236 48
BsiHKAI G_wGCw'C
Cuts at: 0 449 1193 1621 1682 1859 1866
Size: 449 744 428 61 177 7
Fragments arranged by size:
744 449 428 177 61 7
Bsp1286I G_dGCh'C
Cuts at: 0 16 118 214 449 866 959 1193 1621
Size: 16 102 96 235 417 93 234 428
Cuts at: 1621 1682 1759 1859 1866
Size: 61 77 100 7
Fragments arranged by size:
428 417 235 234 102 100 96 93
77 61 16 7
BspHI T'CATG_A
Cuts at: 0 461 680 1866
Size: 461 219 1186
Fragments arranged by size:
1186 461 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 651 1001 1507 1738 1866
Size: 651 350 506 231 128
Fragments arranged by size:
651 506 350 231 128
BsrBI CCG'CTC
Cuts at: 0 1170 1314 1866
Size: 1170 144 552
Fragments arranged by size:
1170 552 144
BsrFI r'CCGG_y
Cuts at: 0 1442 1796 1866
Size: 1442 354 70
Fragments arranged by size:
1442 354 70
BssSI C'ACGA_G
Cuts at: 0 100 1866
Size: 100 1766
BstAPI GCAn_nnn'nTGC
Cuts at: 0 795 1080 1866
Size: 795 285 786
Fragments arranged by size:
795 786 285
BstYI r'GATC_y
Cuts at: 0 653 1322 1367 1375 1866
Size: 653 669 45 8 491
Fragments arranged by size:
669 653 491 45 8
BtgI C'CryG_G
Cuts at: 0 53 836 972 1483 1866
Size: 53 783 136 511 383
Fragments arranged by size:
783 511 383 136 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1853 1866
Size: 1853 13
BtsI GCAGTG_nn'
Cuts at: 0 122 1866
Size: 122 1744
DraIII CAC_nnn'GTG
Cuts at: 0 1776 1866
Size: 1776 90
EaeI y'GGCC_r
Cuts at: 0 839 1602 1866
Size: 839 763 264
Fragments arranged by size:
839 763 264
EarI CTCTTCn'nnn_
Cuts at: 0 817 1823 1828 1866
Size: 817 1006 5 38
Fragments arranged by size:
1006 817 38 5
EcoICRI GAG'CTC
Cuts at: 0 447 1619 1866
Size: 447 1172 247
Fragments arranged by size:
1172 447 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 173 215 433 628 906 958 1195
Size: 164 9 42 218 195 278 52 237
Cuts at: 1195 1219 1338 1474 1866
Size: 24 119 136 392
Fragments arranged by size:
392 278 237 218 195 164 136 119
52 42 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1741 1866
Size: 1741 125
EcoO109I rG'GnC_Cy
Cuts at: 0 105 245 419 900 979 990 1097 1134
Size: 105 140 174 481 79 11 107 37
Cuts at: 1134 1542 1553 1866
Size: 408 11 313
Fragments arranged by size:
481 408 313 174 140 107 105 79
37 11 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 324 1866
Size: 324 1542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 982 1014 1545 1577 1756 1788
Size: 211 32 739 32 531 32 179 32
Cuts at: 1788 1801 1833 1866
Size: 13 32 33
Fragments arranged by size:
739 531 211 179 33 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 666 1725 1866
Size: 666 1059 141
Fragments arranged by size:
1059 666 141
HpaI GTT'AAC
Cuts at: 0 666 1866
Size: 666 1200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1866
Size: 254 1612
MscI TGG'CCA
Cuts at: 0 841 1604 1866
Size: 841 763 262
Fragments arranged by size:
841 763 262
MslI CAynn'nnrTG
Cuts at: 0 55 1714 1866
Size: 55 1659 152
Fragments arranged by size:
1659 152 55
MspA1I CmG'CkG
Cuts at: 0 30 130 474 612 738 786 792 950
Size: 30 100 344 138 126 48 6 158
Cuts at: 950 1077 1485 1659 1866
Size: 127 408 174 207
Fragments arranged by size:
408 344 207 174 158 138 127 126
100 48 30 6
NaeI GCC'GGC
Cuts at: 0 1444 1866
Size: 1444 422
NcoI C'CATG_G
Cuts at: 0 53 836 972 1866
Size: 53 783 136 894
Fragments arranged by size:
894 783 136 53
NgoMIV G'CCGG_C
Cuts at: 0 1442 1866
Size: 1442 424
NspI r_CATG'y
Cuts at: 0 672 1347 1656 1713 1866
Size: 672 675 309 57 153
Fragments arranged by size:
675 672 309 153 57
PasI CC'CwG_GG
Cuts at: 0 111 195 1866
Size: 111 84 1671
Fragments arranged by size:
1671 111 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 365 456 1866
Size: 365 91 1410
Fragments arranged by size:
1410 365 91
PfoI T'CCnGG_A
Cuts at: 0 1061 1866
Size: 1061 805
PpuMI rG'GwC_Cy
Cuts at: 0 245 419 990 1134 1553 1866
Size: 245 174 571 144 419 313
Fragments arranged by size:
571 419 313 245 174 144
PstI C_TGCA'G
Cuts at: 0 791 860 998 1735 1866
Size: 791 69 138 737 131
Fragments arranged by size:
791 737 138 131 69
PvuII CAG'CTG
Cuts at: 0 30 474 612 738 792 950 1077 1866
Size: 30 444 138 126 54 158 127 789
Fragments arranged by size:
789 444 158 138 127 126 54 30
SacI G_AGCT'C
Cuts at: 0 449 1621 1866
Size: 449 1172 245
Fragments arranged by size:
1172 449 245
SacII CC_GC'GG
Cuts at: 0 1486 1866
Size: 1486 380
SanDI GG'GwC_CC
Cuts at: 0 245 419 1866
Size: 245 174 1447
Fragments arranged by size:
1447 245 174
SapI GCTCTTCn'nnn_
Cuts at: 0 1828 1866
Size: 1828 38
SbfI CC_TGCA'GG
Cuts at: 0 860 1866
Size: 860 1006
SfcI C'TryA_G
Cuts at: 0 222 787 856 994 1731 1866
Size: 222 565 69 138 737 135
Fragments arranged by size:
737 565 222 138 135 69
SmaI CCC'GGG
Cuts at: 0 157 1866
Size: 157 1709
SphI G_CATG'C
Cuts at: 0 1656 1866
Size: 1656 210
SrfI GCCC'GGGC
Cuts at: 0 157 1866
Size: 157 1709
StuI AGG'CCT
Cuts at: 0 828 1866
Size: 828 1038
StyI C'CwwG_G
Cuts at: 0 53 836 896 972 1029 1100 1163 1304
Size: 53 783 60 76 57 71 63 141
Cuts at: 1304 1371 1866
Size: 67 495
Fragments arranged by size:
783 495 141 76 71 67 63 60
57 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 859 1208 1866
Size: 859 349 658
Fragments arranged by size:
859 658 349
TatI w'GTAC_w
Cuts at: 0 1475 1535 1866
Size: 1475 60 331
Fragments arranged by size:
1475 331 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 460 492 1866
Size: 460 32 1374
Fragments arranged by size:
1374 460 32
Tth111I GACn'n_nGTC
Cuts at: 0 1045 1866
Size: 1045 821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1170 1866
Size: 1170 696
XmaI C'CCGG_G
Cuts at: 0 155 1866
Size: 155 1711
XmnI GAAnn'nnTTC
Cuts at: 0 1288 1866
Size: 1288 578
Enzymes that do cut:
AarI AcuI AgeI AhdI AleI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbvCI BcgI BciVI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrFI BssSI BstAPI BstYI BtgI BtgZI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I HincII HpaI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI
PstI PvuII SacI SacII SanDI SapI SbfI SfcI
SmaI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EciI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SspI SwaI XbaI XhoI ZraI