(Linear) (Six-base) MAPSORT of: KIBB3077.seq  Check: 6451  from: 1  to: 786



KIBB3077.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0     764     786 
   Size:        764      22
 
AflIII A'CryG_T

Cuts at:      0     530     786 
   Size:        530     256
 
ApaLI G'TGCA_C

Cuts at:      0     473     786 
   Size:        473     313
 
BanI G'GyrC_C

Cuts at:      0     237     786 
   Size:        237     549
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     786 
   Size:         10     776
 
BglI GCCn_nnn'nGGC

Cuts at:      0     641     786 
   Size:        641     145
 
Bme1580I G_kGCm'C

Cuts at:      0     477     786 
   Size:        477     309
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     204     786 
   Size:        204     582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     584     786 
   Size:         80     504     202

  Fragments arranged by size: 

                 504     202      80
 
BsaAI yAC'GTr

Cuts at:      0     472     786 
   Size:        472     314
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     611     641     786 
   Size:        611      30     145

  Fragments arranged by size: 

                 611     145      30
 
BseYI C'CCAG_C

Cuts at:      0     140     786 
   Size:        140     646
 
BsiHKAI G_wGCw'C

Cuts at:      0     477     786 
   Size:        477     309
 
Bsp1286I G_dGCh'C

Cuts at:      0     477     786 
   Size:        477     309
 
BspHI T'CATG_A

Cuts at:      0     482     786 
   Size:        482     304
 
BsrGI T'GTAC_A

Cuts at:      0     527     786 
   Size:        527     259
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      55     786 
   Size:         55     731
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     386     786 
   Size:        386     400
 
BtsI GCAGTG_nn'

Cuts at:      0     120     413     786 
   Size:        120     293     373

  Fragments arranged by size: 

                 373     293     120
 
EaeI y'GGCC_r

Cuts at:      0      27     786 
   Size:         27     759
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     786 
   Size:         79     707
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     165     786 
   Size:        165     621
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     584     786 
   Size:         80     504     202

  Fragments arranged by size: 

                 504     202      80
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     583     786 
   Size:        583     203
 
MscI TGG'CCA

Cuts at:      0      29     786 
   Size:         29     757
 
MslI CAynn'nnrTG

Cuts at:      0     758     786 
   Size:        758      28
 
MspA1I CmG'CkG

Cuts at:      0      33     786 
   Size:         33     753
 
NdeI CA'TA_TG

Cuts at:      0     416     786 
   Size:        416     370
 
NspI r_CATG'y

Cuts at:      0     534     786 
   Size:        534     252
 
PciI A'CATG_T

Cuts at:      0     530     786 
   Size:        530     256
 
PmlI CAC'GTG

Cuts at:      0     472     786 
   Size:        472     314
 
PsiI TTA'TAA

Cuts at:      0     265     786 
   Size:        265     521
 
PstI C_TGCA'G

Cuts at:      0     169     786 
   Size:        169     617
 
SexAI A'CCwGG_T

Cuts at:      0     233     786 
   Size:        233     553
 
SfcI C'TryA_G

Cuts at:      0     165     786 
   Size:        165     621
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     170     786 
   Size:        170     616
 
TatI w'GTAC_w

Cuts at:      0     527     786 
   Size:        527     259
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     611     643     786 
   Size:        611      32     143

  Fragments arranged by size: 

                 611     143      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     520     786 
   Size:        520     266
 

 Enzymes that do cut:

     AccI   AflIII    ApaLI     BanI     BbsI     BglI Bme1580I     BmrI
     BpmI    BsaAI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspHI    BsrGI
   BstAPI    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI     FalI
     MscI     MslI   MspA1I     NdeI     NspI     PciI     PmlI     PsiI
     PstI    SexAI     SfcI    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI