Restriction map for
pF1KB3078
(Linear) (Six-base) MAPSORT of: KIBB3078.seq Check: 6092 from: 1 to: 2823
KIBB3078.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 864 2823
Size: 864 1959
AccI GT'mk_AC
Cuts at: 0 2087 2823
Size: 2087 736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 748 2823
Size: 580 168 2075
Fragments arranged by size:
2075 580 168
AflIII A'CryG_T
Cuts at: 0 812 2784 2823
Size: 812 1972 39
Fragments arranged by size:
1972 812 39
AleI CACnn'nnGTG
Cuts at: 0 2335 2823
Size: 2335 488
AlwNI CAG_nnn'CTG
Cuts at: 0 1670 1788 2750 2823
Size: 1670 118 962 73
Fragments arranged by size:
1670 962 118 73
ApaI G_GGCC'C
Cuts at: 0 1052 2823
Size: 1052 1771
ApaLI G'TGCA_C
Cuts at: 0 877 2823
Size: 877 1946
ApoI r'AATT_y
Cuts at: 0 1279 2823
Size: 1279 1544
AvaI C'yCGr_G
Cuts at: 0 28 109 1471 2138 2814 2823
Size: 28 81 1362 667 676 9
Fragments arranged by size:
1362 676 667 81 28 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1812 1845 2823
Size: 1812 33 978
Fragments arranged by size:
1812 978 33
BamHI G'GATC_C
Cuts at: 0 1151 1378 1621 2823
Size: 1151 227 243 1202
Fragments arranged by size:
1202 1151 243 227
BanI G'GyrC_C
Cuts at: 0 105 133 1142 2823
Size: 105 28 1009 1681
Fragments arranged by size:
1681 1009 105 28
BanII G_rGCy'C
Cuts at: 0 19 962 970 1052 2501 2612 2823
Size: 19 943 8 82 1449 111 211
Fragments arranged by size:
1449 943 211 111 82 19 8
BbeI G_GCGC'C
Cuts at: 0 109 137 2823
Size: 109 28 2686
Fragments arranged by size:
2686 109 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2055 2089 2823
Size: 2055 34 734
Fragments arranged by size:
2055 734 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1840 2823
Size: 1840 983
BclI T'GATC_A
Cuts at: 0 1478 2823
Size: 1478 1345
BglI GCCn_nnn'nGGC
Cuts at: 0 2366 2823
Size: 2366 457
BglII A'GATC_T
Cuts at: 0 1427 2823
Size: 1427 1396
Bme1580I G_kGCm'C
Cuts at: 0 598 881 1052 2823
Size: 598 283 171 1771
Fragments arranged by size:
1771 598 283 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 711 1600 2823
Size: 711 889 1223
Fragments arranged by size:
1223 889 711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1366 1432 1746 1954 2383 2636 2665
Size: 668 698 66 314 208 429 253 29
Cuts at: 2665 2823
Size: 158
Fragments arranged by size:
698 668 429 314 253 208 158 66
29
Bpu10I CC'TnA_GC
Cuts at: 0 964 2823
Size: 964 1859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 2823
Size: 553 2270
BsaI GGTCTCn'nnnn_
Cuts at: 0 1207 2023 2817 2823
Size: 1207 816 794 6
Fragments arranged by size:
1207 816 794 6
BsaBI GATnn'nnATC
Cuts at: 0 2579 2823
Size: 2579 244
BsaHI Gr'CG_yC
Cuts at: 0 106 134 398 2732 2823
Size: 106 28 264 2334 91
Fragments arranged by size:
2334 264 106 91 28
BsaWI w'CCGG_w
Cuts at: 0 908 1250 1517 2823
Size: 908 342 267 1306
Fragments arranged by size:
1306 908 342 267
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 799 829 2823
Size: 799 30 1994
Fragments arranged by size:
1994 799 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 820 2823
Size: 820 2003
BseYI C'CCAG_C
Cuts at: 0 122 151 1051 1182 1899 2585 2823
Size: 122 29 900 131 717 686 238
Fragments arranged by size:
900 717 686 238 131 122 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 2176 2823
Size: 475 1701 647
Fragments arranged by size:
1701 647 475
BsiEI CG_ry'CG
Cuts at: 0 145 1697 2823
Size: 145 1552 1126
Fragments arranged by size:
1552 1126 145
BsiHKAI G_wGCw'C
Cuts at: 0 19 233 881 970 1940 2501 2539 2823
Size: 19 214 648 89 970 561 38 284
Fragments arranged by size:
970 648 561 284 214 89 38 19
Bsp1286I G_dGCh'C
Cuts at: 0 19 233 598 881 962 970 1052 1940
Size: 19 214 365 283 81 8 82 888
Cuts at: 1940 2501 2539 2612 2823
Size: 561 38 73 211
Fragments arranged by size:
888 561 365 283 214 211 82 81
73 38 19 8
BspEI T'CCGG_A
Cuts at: 0 908 1250 2823
Size: 908 342 1573
Fragments arranged by size:
1573 908 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 308 864 2823
Size: 308 556 1959
Fragments arranged by size:
1959 556 308
BsrBI CCG'CTC
Cuts at: 0 165 189 2823
Size: 165 24 2634
Fragments arranged by size:
2634 165 24
BsrDI GCAATG_nn'
Cuts at: 0 422 2823
Size: 422 2401
BsrFI r'CCGG_y
Cuts at: 0 1139 2823
Size: 1139 1684
BssHII G'CGCG_C
Cuts at: 0 61 2823
Size: 61 2762
BstEII G'GTnAC_C
Cuts at: 0 2328 2433 2823
Size: 2328 105 390
Fragments arranged by size:
2328 390 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 1410 2823
Size: 201 1209 1413
Fragments arranged by size:
1413 1209 201
BstYI r'GATC_y
Cuts at: 0 260 1151 1378 1427 1621 2628 2823
Size: 260 891 227 49 194 1007 195
Fragments arranged by size:
1007 891 260 227 195 194 49
BtgI C'CryG_G
Cuts at: 0 10 239 929 1062 1115 1682 2823
Size: 10 229 690 133 53 567 1141
Fragments arranged by size:
1141 690 567 229 133 53 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1077 2371 2823
Size: 1077 1294 452
Fragments arranged by size:
1294 1077 452
BtsI GCAGTG_nn'
Cuts at: 0 1102 1123 2823
Size: 1102 21 1700
Fragments arranged by size:
1700 1102 21
DraI TTT'AAA
Cuts at: 0 2561 2823
Size: 2561 262
DraIII CAC_nnn'GTG
Cuts at: 0 320 2823
Size: 320 2503
EaeI y'GGCC_r
Cuts at: 0 142 203 248 756 1334 1484 2823
Size: 142 61 45 508 578 150 1339
Fragments arranged by size:
1339 578 508 150 142 61 45
EagI C'GGCC_G
Cuts at: 0 142 2823
Size: 142 2681
EarI CTCTTCn'nnn_
Cuts at: 0 1504 2423 2823
Size: 1504 919 400
Fragments arranged by size:
1504 919 400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1912 2823
Size: 1912 911
EcoICRI GAG'CTC
Cuts at: 0 17 968 2499 2823
Size: 17 951 1531 324
Fragments arranged by size:
1531 951 324 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 668 748 1366 1432 1746 1954 2383
Size: 580 88 80 618 66 314 208 429
Cuts at: 2383 2636 2665 2823
Size: 253 29 158
Fragments arranged by size:
618 580 429 314 253 208 158 88
80 66 29
EcoO109I rG'GnC_Cy
Cuts at: 0 113 1048 1667 2367 2823
Size: 113 935 619 700 456
Fragments arranged by size:
935 700 619 456 113
EcoRV GAT'ATC
Cuts at: 0 1914 2823
Size: 1914 909
HaeII r_GCGC'y
Cuts at: 0 109 137 2823
Size: 109 28 2686
Fragments arranged by size:
2686 109 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1530 1562 1613 1645 1898 1930 2632 2664
Size: 1530 32 51 32 253 32 702 32
Cuts at: 2664 2795 2823
Size: 131 28
Fragments arranged by size:
1530 702 253 131 51 32 32 32
32 28
KasI G'GCGC_C
Cuts at: 0 105 133 2823
Size: 105 28 2690
Fragments arranged by size:
2690 105 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 2823
Size: 722 2101
MscI TGG'CCA
Cuts at: 0 205 250 758 1486 2823
Size: 205 45 508 728 1337
Fragments arranged by size:
1337 728 508 205 45
MslI CAynn'nnrTG
Cuts at: 0 946 1537 1954 2077 2335 2783 2823
Size: 946 591 417 123 258 448 40
Fragments arranged by size:
946 591 448 417 258 123 40
MspA1I CmG'CkG
Cuts at: 0 12 90 902 1149 1419 2122 2445 2738
Size: 12 78 812 247 270 703 323 293
Cuts at: 2738 2823
Size: 85
Fragments arranged by size:
812 703 323 293 270 247 85 78
12
NaeI GCC'GGC
Cuts at: 0 1141 2823
Size: 1141 1682
NarI GG'CG_CC
Cuts at: 0 106 134 2823
Size: 106 28 2689
Fragments arranged by size:
2689 106 28
NcoI C'CATG_G
Cuts at: 0 929 1115 2823
Size: 929 186 1708
Fragments arranged by size:
1708 929 186
NdeI CA'TA_TG
Cuts at: 0 1955 2823
Size: 1955 868
NgoMIV G'CCGG_C
Cuts at: 0 1139 2823
Size: 1139 1684
NspI r_CATG'y
Cuts at: 0 816 1132 1812 2624 2788 2823
Size: 816 316 680 812 164 35
Fragments arranged by size:
816 812 680 316 164 35
PciI A'CATG_T
Cuts at: 0 812 2784 2823
Size: 812 1972 39
Fragments arranged by size:
1972 812 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 774 1182 2823
Size: 384 390 408 1641
Fragments arranged by size:
1641 408 390 384
PfoI T'CCnGG_A
Cuts at: 0 1154 1409 1931 2823
Size: 1154 255 522 892
Fragments arranged by size:
1154 892 522 255
PpuMI rG'GwC_Cy
Cuts at: 0 1667 2823
Size: 1667 1156
PspOMI G'GGCC_C
Cuts at: 0 1048 2823
Size: 1048 1775
PstI C_TGCA'G
Cuts at: 0 1109 2194 2450 2823
Size: 1109 1085 256 373
Fragments arranged by size:
1109 1085 373 256
PvuII CAG'CTG
Cuts at: 0 1419 2445 2738 2823
Size: 1419 1026 293 85
Fragments arranged by size:
1419 1026 293 85
RsrII CG'GwC_CG
Cuts at: 0 521 2823
Size: 521 2302
SacI G_AGCT'C
Cuts at: 0 19 970 2501 2823
Size: 19 951 1531 322
Fragments arranged by size:
1531 951 322 19
SacII CC_GC'GG
Cuts at: 0 13 2823
Size: 13 2810
SfcI C'TryA_G
Cuts at: 0 1105 1443 2190 2371 2446 2590 2633 2823
Size: 1105 338 747 181 75 144 43 190
Fragments arranged by size:
1105 747 338 190 181 144 75 43
SfoI GGC'GCC
Cuts at: 0 107 135 2823
Size: 107 28 2688
Fragments arranged by size:
2688 107 28
SmaI CCC'GGG
Cuts at: 0 30 111 2140 2823
Size: 30 81 2029 683
Fragments arranged by size:
2029 683 81 30
SmlI C'TyrA_G
Cuts at: 0 568 2823
Size: 568 2255
SphI G_CATG'C
Cuts at: 0 1132 1812 2823
Size: 1132 680 1011
Fragments arranged by size:
1132 1011 680
SrfI GCCC'GGGC
Cuts at: 0 30 2823
Size: 30 2793
StyI C'CwwG_G
Cuts at: 0 194 206 438 753 929 1115 1374 1572
Size: 194 12 232 315 176 186 259 198
Cuts at: 1572 2823
Size: 1251
Fragments arranged by size:
1251 315 259 232 198 194 186 176
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1966 2316 2823
Size: 1966 350 507
Fragments arranged by size:
1966 507 350
TatI w'GTAC_w
Cuts at: 0 1022 1322 1880 2069 2823
Size: 1022 300 558 189 754
Fragments arranged by size:
1022 754 558 300 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2783 2815 2823
Size: 2783 32 8
Fragments arranged by size:
2783 32 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2724 2823
Size: 2724 99
XmaI C'CCGG_G
Cuts at: 0 28 109 2138 2823
Size: 28 81 2029 685
Fragments arranged by size:
2029 685 81 28
XmnI GAAnn'nnTTC
Cuts at: 0 346 2823
Size: 346 2477
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BcgI
BciVI BclI BglI BglII Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SacI
SacII SfcI SfoI SmaI SmlI SphI SrfI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BbsI BbvCI BfrBI
BlpI BmgBI BmrI BmtI BsaAI BsiWI BsmI BsmBI
BspHI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DrdI EcoNI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI
StuI SwaI Tth111I XbaI XhoI ZraI