Restriction map for
pF1KB3086
(Linear) (Six-base) MAPSORT of: KIBB3086.seq Check: 7270 from: 1 to: 1740
KIBB3086.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 850 1740
Size: 850 890
AclI AA'CG_TT
Cuts at: 0 1670 1740
Size: 1670 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 673 757 1109 1417 1705 1740
Size: 262 411 84 352 308 288 35
Fragments arranged by size:
411 352 308 288 262 84 35
AleI CACnn'nnGTG
Cuts at: 0 451 1740
Size: 451 1289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 286 318 1660 1692 1740
Size: 286 32 1342 32 48
Fragments arranged by size:
1342 286 48 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 696 801 1416 1740
Size: 132 564 105 615 324
Fragments arranged by size:
615 564 324 132 105
ApaI G_GGCC'C
Cuts at: 0 208 1363 1740
Size: 208 1155 377
Fragments arranged by size:
1155 377 208
ApaLI G'TGCA_C
Cuts at: 0 1735 1740
Size: 1735 5
ApoI r'AATT_y
Cuts at: 0 439 627 1740
Size: 439 188 1113
Fragments arranged by size:
1113 439 188
AvaI C'yCGr_G
Cuts at: 0 60 1663 1740
Size: 60 1603 77
Fragments arranged by size:
1603 77 60
BanI G'GyrC_C
Cuts at: 0 153 1740
Size: 153 1587
BanII G_rGCy'C
Cuts at: 0 208 299 1363 1740
Size: 208 91 1064 377
Fragments arranged by size:
1064 377 208 91
BclI T'GATC_A
Cuts at: 0 716 729 1740
Size: 716 13 1011
Fragments arranged by size:
1011 716 13
BglII A'GATC_T
Cuts at: 0 1072 1092 1740
Size: 1072 20 648
Fragments arranged by size:
1072 648 20
BlpI GC'TnA_GC
Cuts at: 0 46 1740
Size: 46 1694
Bme1580I G_kGCm'C
Cuts at: 0 208 1050 1363 1739 1740
Size: 208 842 313 376 1
Fragments arranged by size:
842 376 313 208 1
BmgBI CAC'GTC
Cuts at: 0 607 1740
Size: 607 1133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 1740
Size: 906 834
Bpu10I CC'TnA_GC
Cuts at: 0 1364 1740
Size: 1364 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 204 283 1740
Size: 130 74 79 1457
Fragments arranged by size:
1457 130 79 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 970 1740
Size: 970 770
BsaHI Gr'CG_yC
Cuts at: 0 95 1740
Size: 95 1645
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 286 316 1740
Size: 286 30 1424
Fragments arranged by size:
1424 286 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 611 1740
Size: 611 1129
BseYI C'CCAG_C
Cuts at: 0 539 1740
Size: 539 1201
BsiHKAI G_wGCw'C
Cuts at: 0 299 1739 1740
Size: 299 1440 1
Fragments arranged by size:
1440 299 1
BsmI GAATG_Cn'
Cuts at: 0 324 767 1014 1740
Size: 324 443 247 726
Fragments arranged by size:
726 443 324 247
Bsp1286I G_dGCh'C
Cuts at: 0 208 299 1050 1363 1739 1740
Size: 208 91 751 313 376 1
Fragments arranged by size:
751 376 313 208 91 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 850 1740
Size: 850 890
BsrBI CCG'CTC
Cuts at: 0 116 1740
Size: 116 1624
BsrDI GCAATG_nn'
Cuts at: 0 840 1740
Size: 840 900
BsrGI T'GTAC_A
Cuts at: 0 929 1740
Size: 929 811
BssSI C'ACGA_G
Cuts at: 0 1282 1740
Size: 1282 458
BstAPI GCAn_nnn'nTGC
Cuts at: 0 23 1740
Size: 23 1717
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1713 1740
Size: 1713 27
BstYI r'GATC_y
Cuts at: 0 680 1072 1092 1740
Size: 680 392 20 648
Fragments arranged by size:
680 648 392 20
Bsu36I CC'TnA_GG
Cuts at: 0 200 1740
Size: 200 1540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 52 1740
Size: 52 1688
BtsI GCAGTG_nn'
Cuts at: 0 664 1740
Size: 664 1076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1014 1049 1740
Size: 1014 35 691
Fragments arranged by size:
1014 691 35
DraIII CAC_nnn'GTG
Cuts at: 0 23 1740
Size: 23 1717
EaeI y'GGCC_r
Cuts at: 0 2 756 1703 1740
Size: 2 754 947 37
Fragments arranged by size:
947 754 37 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1261 1740
Size: 1261 479
EcoICRI GAG'CTC
Cuts at: 0 297 1740
Size: 297 1443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 673 757 906 1109 1417 1705 1740
Size: 262 411 84 149 203 308 288 35
Fragments arranged by size:
411 308 288 262 203 149 84 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1465 1740
Size: 1465 275
EcoO109I rG'GnC_Cy
Cuts at: 0 204 1359 1360 1740
Size: 204 1155 1 380
Fragments arranged by size:
1155 380 204 1
FspI TGC'GCA
Cuts at: 0 16 1740
Size: 16 1724
FspAI rTGC'GCAy
Cuts at: 0 16 1740
Size: 16 1724
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1265 1297 1740
Size: 1265 32 443
Fragments arranged by size:
1265 443 32
HindIII A'AGCT_T
Cuts at: 0 419 637 1687 1740
Size: 419 218 1050 53
Fragments arranged by size:
1050 419 218 53
MscI TGG'CCA
Cuts at: 0 1705 1740
Size: 1705 35
MslI CAynn'nnrTG
Cuts at: 0 451 772 1429 1699 1711 1740
Size: 451 321 657 270 12 29
Fragments arranged by size:
657 451 321 270 29 12
MspA1I CmG'CkG
Cuts at: 0 273 1683 1740
Size: 273 1410 57
Fragments arranged by size:
1410 273 57
NspI r_CATG'y
Cuts at: 0 215 930 1740
Size: 215 715 810
Fragments arranged by size:
810 715 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 776 1740
Size: 776 964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 1740
Size: 286 32 1422
Fragments arranged by size:
1422 286 32
PspOMI G'GGCC_C
Cuts at: 0 204 1359 1740
Size: 204 1155 381
Fragments arranged by size:
1155 381 204
PstI C_TGCA'G
Cuts at: 0 137 1231 1740
Size: 137 1094 509
Fragments arranged by size:
1094 509 137
PvuII CAG'CTG
Cuts at: 0 273 1740
Size: 273 1467
SacI G_AGCT'C
Cuts at: 0 299 1740
Size: 299 1441
ScaI AGT'ACT
Cuts at: 0 883 1740
Size: 883 857
SfcI C'TryA_G
Cuts at: 0 133 1203 1227 1740
Size: 133 1070 24 513
Fragments arranged by size:
1070 513 133 24
SmaI CCC'GGG
Cuts at: 0 62 1740
Size: 62 1678
SmlI C'TyrA_G
Cuts at: 0 145 219 298 1740
Size: 145 74 79 1442
Fragments arranged by size:
1442 145 79 74
StuI AGG'CCT
Cuts at: 0 340 557 1740
Size: 340 217 1183
Fragments arranged by size:
1183 340 217
StyI C'CwwG_G
Cuts at: 0 572 1740
Size: 572 1168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 424 1740
Size: 141 283 1316
Fragments arranged by size:
1316 283 141
TatI w'GTAC_w
Cuts at: 0 881 929 1740
Size: 881 48 811
Fragments arranged by size:
881 811 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 698 730 1740
Size: 698 32 1010
Fragments arranged by size:
1010 698 32
Tth111I GACn'n_nGTC
Cuts at: 0 496 1740
Size: 496 1244
XmaI C'CCGG_G
Cuts at: 0 60 1740
Size: 60 1680
XmnI GAAnn'nnTTC
Cuts at: 0 896 1172 1189 1740
Size: 896 276 17 551
Fragments arranged by size:
896 551 276 17
Enzymes that do cut:
AarI AclI AcuI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BclI BglII BlpI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgZI BtsI CspCI
DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
FspAI Hin4I HindIII MscI MslI MspA1I NspI PflMI
PpiI PspOMI PstI PvuII SacI ScaI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BmrI BmtI
BplI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrFI BssHII BstBI BstEII BstZ17I BtgI
ClaI DraI DrdI EagI EarI EcoRI EcoRV FalI
FseI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XcmI XhoI ZraI